Method for establishing in-vitro regeneration system of rachises of heuchera micrantha

An in vitro regeneration, inflorescence rachis technology, applied in the direction of plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of difficulty in establishing sterile system, easy to form browning, and high pollution rate, and achieve a beneficial effect on microbial activity. , Improve the nutritional environment, the effect of good ventilation

Active Publication Date: 2019-08-16
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tissue culture rapid propagation system is limited by the explants of the female parent. The contamination rate of the explants is too high after simple disinfection, and browning is easy to occur after excessive disinfection. It is difficult to establish a sterile line

Method used

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  • Method for establishing in-vitro regeneration system of rachises of heuchera micrantha

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Explant Screening Example:

[0031] 1. Screening of the placement mode of explants in the culture medium

[0032] For the inflorescence axis of explants with similar size and growth state, the different placement methods also lead to different culture effects. The explants placed on an inclined plane germinated early, had a large number, and grew extremely fast, while the inflorescence axis placed on a flat surface had almost no bud point differentiation, and the explants placed more than 35 days tended to brown or dry up gradually.

[0033] Table 1

[0034]

[0035] 2. Examples of medium screening

[0036] During the establishment of the in vitro regeneration system of the callus pathway, the success of callus differentiation is the key step of the regeneration system. The rate of callus differentiation directly affects whether the regenerative tissue culture system is feasible. The combination of basic medium and hormone types and concentrations in the callus d...

Embodiment 2

[0040] 1. Selection and disinfection of explants: select the inflorescence rachis that has grown for about 20 days as the explants, wash them clean, and then disinfect them. The disinfection process is: rinse them with clean water, rinse them once with Amway detergent, and shake them on a shaker for 15 minutes. -20min; after rinsing with running water for 20-30min, disinfect with 75% ethanol for 10-20s on the aseptic operating table, immediately pour 0.1% mercuric chloride for disinfection for 12min, and finally wash 4 times with sterile water, shaking 3- After 5 minutes, the sterilized inflorescence rachis was cut into 2 cm long segments, and 50 inflorescence rachis segments were inserted in total.

[0041] 2. Induction of callus: take the explant fragments obtained in step (1), and inoculate them in the induction medium (invert the slant of the medium in advance) on a slant to induce callus. The culture temperature is 25±2°C, first cultured in the dark for 10 days, and then ...

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Abstract

The invention provides a method for establishing an in-vitro regeneration system of rachises of heuchera micrantha. The method is characterized by comprising steps as follows: selection and disinfection of an explant, induced differentiation of callus, proliferation of adventitious buds, root induction, seedling hardening and transplanting of tissue culture seedlings. By means of the method, a large number of aseptic seedlings of heuchera micrantha can be obtained, the problem of rapid propagation of the heuchera micrantha is solved, the heuchera micrantha is propagated by 300 times or more within one culture cycle, the survival rate also reaches 98.71%, increase of the proliferation rate and the transplanting survival rate greatly fills up the blank of the market, and the method has broadapplication prospects.

Description

technical field [0001] The present invention relates to alum root ( Heuchera micrantha ) tissue culture propagation method, which belongs to the technical field of seedling propagation of herbaceous ornamental plants, and specifically relates to a method for establishing an alum root inflorescence axis in vitro regeneration system. Background technique [0002] Heuchera micrantha (Heuchera micrantha), also known as coral bell, is a perennial herbaceous flower of the saxifrage family Heuchera micrantha. Shallow roots. The leaves are base-shaped, broad-hearted, 20-25cm long, dark purple, evergreen in warm areas, small, bell-shaped flowers, 0.6-1.2cm in diameter, red, symmetrical on both sides, prefer partial shade, and tolerate full light; it is often used in gardens It can be used in forest flower borders, ground cover, garden greening and so on. The flowering period is from April to October. It is an ideal perennial flower border material. It is also a perennial flower ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 束晓春王忠严冬琴李乃伟郑玉红庄维兵张凤姣吴尤宏
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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