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Preparation method of medium for rapid breeding of Photinia fraseri

A medium preparation and culture medium technology, which is applied in the field of preparation of a medium for rapid propagation of rhododendron, can solve the problems of low reproduction coefficient, time-consuming and labor-intensive, and great influence on the growth of the mother tree, so as to increase the number of reproductions, reduce the cost, and accelerate the reproduction. effect of speed

Inactive Publication Date: 2012-04-25
SHANXI TENGDA SEED
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Problems solved by technology

[0003] First, this method needs a large amount of propagation materials. Second, it has a great impact on the growth of the mother tree that is taken from the material, which is not conducive to the cultivation of the tree shape. Third, when cutting the cuttings, pay attention to keeping the upper incision at a distance of 1 cm from the top bud, which is time-consuming and labor-intensive. Fourth, The reproduction coefficient is low, generally 30% in foreign countries, but only 20% in China

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Embodiment Construction

[0014] The present invention can be better understood from the following examples. However, it is easily understood by those skilled in the art that the examples are only for illustrating the present invention, and should not and will not limit the present invention described in detail in the claims.

[0015] From April 2009 to December 2010, in the Tissue Culture Laboratory of the Institute of Crop Science, Shanxi Academy of Agricultural Sciences, after repeated experiments, researchers obtained the tissue culture and rapid propagation technology of Photinia fragrans, and successfully cultivated it into tissue culture seedlings. The main process is as follows: in the morning of a sunny summer day in 2009, collect semi-lignified new shoots as explants, remove excess stems and leaves, put them in a beaker with 0.1% detergent solution, soak for 15 minutes, stir constantly during the period, and put the beaker Cover the mouth with gauze and tie it tightly, rinse with tap water fo...

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Abstract

A preparation method of a medium for rapid breeding of Photinia fraseri belongs to the technical field of plant cultivation and comprises steps of initial medium preparation, enrichment medium preparation and rooting medium preparation. The initial medium is prepared by using MS as a basal medium with the addition of 2.0mg / L of 6-BA, 0.15mg / L of NAA, 0.1% of active carbon, 3% of cane sugar and 0.65% of an agar powder at pH 5.8. The enrichment medium is prepared by using half of MS as a basal medium with the addition of 2.5mg / L of 6-BA, 0.5mg / L of NAA, 3% of cane sugar and 0.65% of an agar powder at pH 5.8. The rooting medium is prepared by using half of MS as a basal medium with the addition of 1.5mg / L of IBA, 0.2mg / L of NAA, 1.5% of cane sugar and 0.65% of an agar powder at pH 5.8. The preparation method provided by the invention can be used to greatly increase the breeding quantity, accelerate the breeding speed and reduce the cost.

Description

technical field [0001] The invention belongs to the field of asexual propagation of trees, and in particular relates to a preparation method of a rapid breeding medium for Photinia fruticosa. Background technique [0002] Photinia fragrans is mostly propagated by cuttings in production. Cuttages generally use branches as propagation materials. The traditional method is to select thick lignified or semi-lignified branches of the year, and cut cuttings about 8-15cm long. The incision is smooth and the upper incision distance The terminal bud is about 1cm, cut off half of the leaves on the top of the strip, and cut off the rest of the leaves. The base of the cut cuttings is treated with a rooting agent and inserted into the substrate to wait for rooting. [0003] First, this method needs a large amount of propagation materials, second, it has a great influence on the growth of the mother tree that is taken, and is unfavorable for tree shape cultivation. Third, it is time-consum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王美霞张爱莲王创云侯雅静李海燕张丽娜秦作霞
Owner SHANXI TENGDA SEED
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