Application of rhodotorula glutinis deacetylase protein 2 for improving mitochondrial functions

Abstract

The invention discloses application of sirtuin2 protein for improving mitochondrial functions and an action mechanism, which belong to the technical field of biology. The rhodotorula glutinis sirtuin2gene with the functions of reconstructing a mitochondrial network and improving the phenotype of mammalian senescent cells is recombined and expressed in mammalian cells. Experimental results show that the expression levels of fusion proteins Mfn1, Mfn2 and the division protein Drp1 can be improved, the SA-beta-gal activity level of mammalian cells is reduced, the expression level of CK19 and beta1 integrin is enhanced, the intracellular ROS level is reduced, the intracellular ATP level is obviously improved, the mitochondrial morphology is improved, the mitochondrial network is improved, theanti-aging capability of the cells is improved, and a new strategy is provided for treating mitochondrial dysfunction and preventing aging by using the sirtuin2 protein.

Description

technical field [0001] The invention belongs to the field of biological technology of strain protein application mechanism. More specifically, the present invention relates to the mechanism and application of a Rhodotorula viscosus sirtuin2 protein expressed by mammalian cells, which has the function of remodeling the mitochondrial network and improving the phenotype of aging mammalian cells. Background technique [0002] Evidence that Sirtuins genes in lower eukaryotes are related to lifespan provides a basis for mammalian Sirtuins genes to affect lifespan in mammals. Various experiments have proved that SIRT1 can be used for anti-aging. Recent studies have shown that brain-specific SIRT1 overexpression can prolong lifespan and delay aging in mice. Embryo development and postnatal life are severely impaired in inbred mice lacking SIRT1. A recent study confirmed that outbred mice lacking SIRT1 do not extend their lifespan through energy restriction. This suggests that en...

AI Technical Summary

Problems solved by technology

Aging is associated with loss of mitochondrial network homeostasis, but the cellular processes of biological aging associated with these changes remain unclear

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