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Application of rhodotorula glutinis deacetylase protein 2 for improving mitochondrial functions

A mitochondrial and protein technology, applied in the biological field of strain protein application mechanism, can solve problems such as unclear cell processes

Active Publication Date: 2020-03-13
广州弘润生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aging is associated with loss of mitochondrial network homeostasis, but the cellular processes of biological aging associated with these changes remain unclear

Method used

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  • Application of rhodotorula glutinis deacetylase protein 2 for improving mitochondrial functions
  • Application of rhodotorula glutinis deacetylase protein 2 for improving mitochondrial functions
  • Application of rhodotorula glutinis deacetylase protein 2 for improving mitochondrial functions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0046] Example Rhodotorula viscosus Sir2 homologous gene sirtuin2 Effects on the phenotype and mitochondrial remodeling of aging HaCaT cells

[0047] 1. Experimental materials

[0048] 1.1 Strains, plasmids and cells

[0049] Escherichia coli E. coli DH5α, epidermal keratinocytes HaCaT are all preserved in the laboratory; plasmids are pFlag-CMV-2 and pFlag-CMV-2- Rt-sirtuin2 and pFlag-CMV-2- Rt-sirtuin4 Constructed by Shanghai Jikai Gene Company.

[0050] main reagent

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PUM

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Abstract

The invention discloses application of sirtuin2 protein for improving mitochondrial functions and an action mechanism, which belong to the technical field of biology. The rhodotorula glutinis sirtuin2gene with the functions of reconstructing a mitochondrial network and improving the phenotype of mammalian senescent cells is recombined and expressed in mammalian cells. Experimental results show that the expression levels of fusion proteins Mfn1, Mfn2 and the division protein Drp1 can be improved, the SA-beta-gal activity level of mammalian cells is reduced, the expression level of CK19 and beta1 integrin is enhanced, the intracellular ROS level is reduced, the intracellular ATP level is obviously improved, the mitochondrial morphology is improved, the mitochondrial network is improved, theanti-aging capability of the cells is improved, and a new strategy is provided for treating mitochondrial dysfunction and preventing aging by using the sirtuin2 protein.

Description

technical field [0001] The invention belongs to the field of biological technology of strain protein application mechanism. More specifically, the present invention relates to the mechanism and application of a Rhodotorula viscosus sirtuin2 protein expressed by mammalian cells, which has the function of remodeling the mitochondrial network and improving the phenotype of aging mammalian cells. Background technique [0002] Evidence that Sirtuins genes in lower eukaryotes are related to lifespan provides a basis for mammalian Sirtuins genes to affect lifespan in mammals. Various experiments have proved that SIRT1 can be used for anti-aging. Recent studies have shown that brain-specific SIRT1 overexpression can prolong lifespan and delay aging in mice. Embryo development and postnatal life are severely impaired in inbred mice lacking SIRT1. A recent study confirmed that outbred mice lacking SIRT1 do not extend their lifespan through energy restriction. This suggests that en...

Claims

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Application Information

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IPC IPC(8): G01N33/68A61K38/16A61P39/06
CPCG01N33/68A61K38/168A61P39/06
Inventor 孙晗笑刘梦鸽利时雨
Owner 广州弘润生物科技有限公司
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