Application of rhodotorula glutinis deacetylase protein 4 for improving mitochondrial functions

A mitochondria and protein technology, applied in the biological field of bacterial protein application mechanism, can solve the problem of unclear cell process and so on

Pending Publication Date: 2020-03-13
广州弘润生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aging is associated with loss of mitochondrial network homeostasis, but the cellular processes of biological aging associated with these changes remain unclear

Method used

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  • Application of rhodotorula glutinis deacetylase protein 4 for improving mitochondrial functions
  • Application of rhodotorula glutinis deacetylase protein 4 for improving mitochondrial functions
  • Application of rhodotorula glutinis deacetylase protein 4 for improving mitochondrial functions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0046] Example Rhodotorula viscosus Sir2 homologous gene sirtuin4 Effects on the phenotype and mitochondrial remodeling of aging HaCaT cells

[0047] 1. Experimental materials

[0048] 1.1 Strains, plasmids and cells

[0049] Escherichia coli E. coli DH5α, epidermal keratinocytes HaCaT are all preserved in the laboratory; plasmids are pFlag-CMV-2 and pFlag-CMV-2- Rt-sirtuin2 and pFlag-CMV-2- Rt-sirtuin4 Constructed by Shanghai Jikai Gene Company.

[0050] main reagent

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PUM

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Abstract

The invention discloses an application of sirtuin4 protein for improving mitochondrial functions and an action mechanism thereof, which belong to the technical field of biology. According to the invention, the rhodotorula glutinis sirtuin4 gene with the functions of reconstructing a mitochondrial network and improving the phenotype of mammalian senescent cells is recombined and expressed in mammalian cells. The experimental results show that the recombinant protein can activate related proteins of damaged mitochondrial autophagy; the SA-beta-gal activity level of mammalian cells is obviously reduced; the expression level of CK19 and beta1 integrin is enhanced; the sirtuin4 protein can be used for reducing the intracellular ROS level, obviously improving the intracellular ATP level and improving the mitochondrial morphology, so that the mitochondrial network is improved, the anti-aging capability of the cells is improved, and a new strategy is provided for treating mitochondrial dysfunction and preventing aging by using the sirtuin4 protein.

Description

technical field [0001] The invention belongs to the field of biological technology of strain protein application mechanism. More specifically, the present invention relates to the mechanism and application of a Rhodotorula viscosus sirtuin4 protein expressed by mammalian cells, which has the function of remodeling the mitochondrial network and improving the phenotype of aging mammalian cells. Background technique [0002] Evidence that Sirtuins genes in lower eukaryotes are related to lifespan provides a basis for mammalian Sirtuins genes to affect lifespan in mammals. Various experiments have proved that SIRT1 can be used for anti-aging. Recent studies have shown that brain-specific SIRT1 overexpression can prolong lifespan and delay aging in mice. Embryo development and postnatal life are severely impaired in inbred mice lacking SIRT1. A recent study confirmed that outbred mice lacking SIRT1 do not extend their lifespan through energy restriction. This suggests that en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/80C12N15/85C12N5/10A23L33/195A23K20/147A61K38/17A61P3/00
CPCC12N9/80C12Y305/01098C12N15/85A23L33/195A23K20/147A61K38/1709A61P3/00A23V2002/00A23V2200/302A23V2250/546
Inventor 孙晗笑刘梦鸽利时雨
Owner 广州弘润生物科技有限公司
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