Method for preparing hydrogen gas

A technology for the production of hydrogen, microorganisms, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc.

Inactive Publication Date: 2008-10-29
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, hydrogen mainly comes from the reforming conversion of fossil fuels (accounting for 96% of hydrogen sources) and electrolysis of water to produce hydrogen (accounting for 4%), which obviously failed to get rid of the original fossil energy system

Method used

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  • Method for preparing hydrogen gas
  • Method for preparing hydrogen gas
  • Method for preparing hydrogen gas

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, adopt basal culture medium to produce hydrogen

[0036] The pH of the basal medium (glucose 10.0 g / L, tryptone 5.0 g / L, ammonium sulfate 2.0 g / L, magnesium sulfate 0.2 g / L) was adjusted to 6.0. Enter the activated Enterobacter aerogenes (Enterobacter aerogenes IAM1183) in the culture medium, so that the initial content of Enterobacter aerogenes IAM1183 is 5×10 8 CFU / ml, cultured at 37°C, 170rpm for 15h. During the cultivation process, anaerobic conditions were adopted, and oxygen in the air was removed by nitrogen purging. During the cultivation process, the generated gas was collected. After the cultivation, the total amount of hydrogen gas generated was calculated, and the remaining content of glucose in the medium was detected at the same time.

[0037] The measurement of hydrogen adopts the method of gas chromatography (GC112A model gas chromatograph of Shanghai Precision Scientific Instrument Co., Ltd.; TCD detector; TDX-01 stainless steel packed co...

Embodiment 2

[0040] Embodiment 2, adopt the culture medium that adds inorganic phosphate to produce hydrogen

[0041] The pH of the basal medium (glucose 10.0 g / L, tryptone 5.0 g / L, ammonium sulfate 2.0 g / L, magnesium sulfate 0.2 g / L) was adjusted to 6.0. Add different amounts of sodium dihydrogen phosphate to the basal medium, so that the concentration of inorganic phosphate in each medium is 0, 0.02, 0.05, 0.1, 0.15, 0.2, 0.25, 0.3 mol / L. Each culture medium with different inorganic phosphate content was respectively inoculated with Enterobacter aerogenes IAM1183, so that the initial content of Enterobacter aerogenes IAM1183 was 5×10 8 CFU / ml, cultured at 37°C, 170rpm for 15h. During the cultivation process, anaerobic conditions were adopted, and oxygen in the air was removed by nitrogen purging. Collect the gas produced during the cultivation of each culture medium, calculate the total amount of hydrogen generated after the culture, and detect the glucose content in each culture mediu...

Embodiment 3

[0046] Embodiment 3, adopt the substratum that adds pyrophosphate to produce hydrogen

[0047] The pH of the basal medium (glucose 10.0 g / L, tryptone 5.0 g / L, ammonium sulfate 2.0 g / L, magnesium sulfate 0.2 g / L) was adjusted to 6.0. Add different amounts of sodium pyrophosphate to the basal medium to make the concentration of pyrophosphate in the medium respectively 0, 0.02, 0.05, 0.1, 0.15, 0.2, 0.25, 0.3 mol / L. Each medium with different content of pyrophosphate was inoculated with Enterobacter aerogenes IAM1183, so that the initial content of Enterobacter aerogenes IAM1183 was 5×10 8 CFU / ml, cultured at 37°C, 170rpm for 15h. During the cultivation process, anaerobic conditions were adopted, and oxygen in the air was removed by nitrogen purging. Collect the gas produced during the cultivation of each culture medium, calculate the total amount of hydrogen generated after the culture, and detect the glucose content in each culture medium at the same time.

[0048] The assay...

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Abstract

The invention discloses a method for producing hydrogen by culturing hydrogen-producing microbes in a culture medium containing phosphate oils. Phosphate is added in a cellular environment to simultaneously promote rapid growth and rapid recovery of ATP, thus controlling the ATP level and oxidization-reduction potential in cells, and accelerating consumption of carbon source and the production efficiency and hydrogen. Additionally, the phosphate can be recycled in the culture system without contaminating the environment. By using the method of the invention, the hydrogen production rate is improved by more than three times, the sugar utilization rate is improved by five times, and the hydrogen transformation efficiency is remarkably improved.

Description

technical field [0001] The invention belongs to the field of biological energy, and in particular relates to a method for producing hydrogen. Background technique [0002] The fossil energy system established since the Industrial Revolution in the 18th century is facing two major challenges: the non-renewability of fossil fuels such as coal, oil, and natural gas; and environmental problems such as the greenhouse effect, acid rain, and dust pollution caused by the petroleum refining industry. These have greatly promoted the research and development of wind energy, solar energy, water energy, biomass energy, geothermal energy, ocean energy and other renewable energy industries. [0003] Biomass energy has always been an important energy source for human survival. It ranks fourth in the world's total energy consumption after coal, oil and natural gas, and occupies an important position in the entire energy system. In my country, biomass energy accounts for 20% of the total ene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/01
Inventor 邢新会卢元赵洪新张翀马堃来奇恒
Owner TSINGHUA UNIV
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