80 results about "Enterobacter aerogenes" patented technology

The invention discloses an enterobacteria aerogenes and the application. The inventor screens Enterobacter aerogenes XM02 by a great deal of experimental researches. The bacterial has ferrum redactum active, facultative and electron donor, which can survive in the aerobic or anaerobic environment, wherein the electron donor in the anaerobic environment has the wide application, the electron donor which can electron donor comprises glycerin, citrate, dextrose, sucrose or the like, the ferric iron which can deacidize comprises szomolnokite, goethite, lepidocrocite, bloodstone, yellow copiapite or the like.

The invention belongs to the field of examination of microorganisms, and relates to a method for quickly identifying bloodstream infection bacteria by a high-resolution fusion curve method. The method comprises the following steps of: performing smear gram staining on a blood culture positive culture flask to distinguish negative bacteria and positive bacteria; selectively amplifying different 16S fragments for the negative bacteria and the positive bacteria; and analyzing by the high-resolution fusion curve method. Common bloodstream infection pathogenic bacteria comprise staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, enterobacter cloacae, Serratia marcescens, Klebsiella oxytoca, acinetobacter lwoffii, enterobacter aerogenes, stenotrophomonas maltophilia and burkholderia cepacia. By the method, the common bloodstream infection pathogenic bacteria can be measured quickly, efficiently and accurately, so that strong evidences are provided and precious time is striven for subsequent treatment.

The invention discloses a method for acquiring microbes capable of degrading octachlorodipropyl ether from soil or sludge, and a high-efficiency octachlorodipropyl ether-degrading bacterium According to the method, sludge or soil containing octachlorodipropyl ether and acquired from a petrochemical factory, a mosquito-repellent incense factory, farmland or other places is used as a sample, and culture of a microbial strain, separating, purifying, domestication and screening are successively carried out so as to select out the octachlorodipropyl ether-degrading bacterium. The method provided by the invention extracts from soil and sludge an Enterobacter aerogenes strain M2016517 with high octachlorodipropyl ether degradability, and the Enterobacter aerogenes strain M2016517 is preserved in Guangdong Microbiological Culture Collection Center on May 17, 2016, with an accession number of GDMCC No. 60041. The octachlorodipropyl ether degradation rate of the strain is as high as 95%. The method provided by the invention can achieve the purposes of screening and domestication of the strain at the same time, so the domestication period is obviously shortened; moreover, the method is simple, convenient and efficient, and the screened octachlorodipropyl ether-degrading bacterium has high octachlorodipropyl ether degradation rate.

The invention provides application of a microbe strain-Enterobacter aerogenes capable of degrading various pyrethroid pesticides simultaneously to the degrading of pyrethroid pesticide residue, and a preparation for degrading the pyrethroid pesticide residue prepared by the strain. An efficient degrading strain is obtained through screening to simultaneously degrade three pyrethroid pesticide residues, namely bifenthrin, fenpropathrin and cypermethrin, and is analyzed and researched in degrading characteristic, so as to disclose the basic rule of the strain for degrading the pesticide and provide scientific basis for controlling pyrethroid pesticide residue.

The invention discloses a molecular beacon and a kit for quickly identifying various clinically common bacteria and belongs to the technical field of microbiological detection. The sequences SEQ ID of the molecular beacon are as shown in v6p1, v6p2, v1p1 and v1r. Through comparison between various clinically common bacteria 16s ribosome RNA sequences in an NCBI gene sequence database and design of the molecular beacon at a tag sequence, clinically common 18 bacteria, including baumanii, A.hydrophila, burkholderia cepacia, citrobacter freundii, enterobacter cloacae, enterococcus faecium, enterococcus faecalis, enterobacter aerogenes, escherichia coli, klebsiella pneumoniae, listeria monocytogenes, proteus mirabilis, pseudomonas aeruginosa, staphylococcus epidermidis, staphylococcus aureus, salmonella, serratia marcescens and stenotrophomonas maltophilia, can be quickly identified. The invention further discloses the kit containing the molecular beacon and used for detecting various bacteria, and the kit can quickly and accurately identify various clinically common bacteria.

The invention discloses a gene chip for detecting 15 clinical common pathogenic microorganisms. The gene chip comprises specific detection probes fixed on a solid phase vector, wherein the probes are designed for 16SrRNA gene and ITS gene sequences of staphylococcus aureus, klebsiella pneumoniae, pseudomonas aeruginosa, proteus mirabilis, Escherichia coli, shigella, enterobacter aerogenes, pseudomonas fluorescens, candida albicans, candida glabrata, candida tropicalis, candida parapsilosis, aspergillus terreus, aspergillus flavus and rhizopus oryzae, and screened and verified by repeated tests, and have high hybridization specificity and accuracy. The chip provided by the invention ensures simple detection operations and relatively lower cost, saves time, is applied to the screening of the clinical common pathogenic microorganisms, the early rapid diagnosis of critical patients and the diagnosis of suddenly occurring infection event pathogens and favorable for clinically rapidly diagnosing infection sources and functions in the early detection and early diagnosis of clinical diseases.

Described herein are antimicrobial articles having improved antimicrobial efficacy and antireflective properties. Further described are methods of making and using the improved articles. The antimicrobial articles generally include an antimicrobial element and an antireflective element. The antireflective element, in some cases, can be disposed directly on a glass, glass-ceramic or ceramic substrate and the antimicrobial element is disposed on the antireflective element. The article can exhibit a reflectance of about 4% or less (and less than 1% in some cases) in the range of about 425 nm to about 725 nm. Further, the article can be characterized with an antimicrobial efficacy by exhibiting at least a 2 log reduction in a concentration of at least Staphylococcus aureus, Enterobacter aerogenes, and Pseudomonas aeruginosa bacteria under a Modified EPA Copper Test Protocol.

The invention relates to an Aerobacter enterobacteria which can excrete algaelysing substances and its application in controlling blue-algae water bloom. The said strain is a bacteria which can excrete algaelysing substances and is obtained by thickening the natural water sample 10-100 times to perform algaelysing experiment separation, which is thought as Aerobacter enterobacteria W5 by biochemical determination. The white powder preparation can be obtained after the fermentation by the spray drying of Aerobacter enterobacteria, and the algaelysing substances all can effectively kill the water bloom blue algae in an application concentration of 2-200ppm. Furthermore, the said preparation is of convenient use, is safety and nontoxic, and is no harm to the environment. The condition of fermentation of the said Aerobacter enterobacteria is: the sterilizing parameter is 121 DEG C and 20-30 minutes; the fermentation parameter is: the pH is 5.8.0-7.0 and DO is 70-90, the fermentation temperature is 34.0-39.0 DEG C, the mixing speed id 70-130 r/minute, and the fermentation time is 16-48 minutes. The fermentation culture medium formulation is: in the fermentation process there is no need to supplement the materials and to adjust the PH, and the spray drying condition of the fermentation products is that the import temperature is 198-210 DEG C and the export temperature is 68-79 DEG C.

The invention discloses an Enterobacter aerogenes S27 for degrading malachite green. The Enterobacter aerogenes with the preservation number CCTCC NO: M 2017452 is preserved on Aug. 28, 2017 in ChinaCenter For Type Culture Collection in Wuhan University in Wuhan province of China. The Enterobacter aerogenes resists salt, is able to adapt to harsh environment, is low-cost in use, can recover ecological balance, and is applicable to degradation of the malachite green in wastewater, thereby serving as a beneficial strain resource for environment pollution caused by the malachite green.

The invention belongs to the field of quality control of microbiological detection, and particularly relates to a sample for verifying capacity of total coliform flora in domestic drinking water and a preparation method thereof. The sample for verifying the capacity of the total coliform flora in the domestic drinking water comprises target flora and background flora, wherein the target flora consists of escherichia coli, klebsiella pneumoniae, enterobacter cloacae, proteus mirabilis and enterobacter aerogenes; the background flora consists of staphylococcus aureus and bacillus cereus. The uniformity and the stability of the sample meet capacity verifying requirements, so that the stability of a test sample can be ensured to the maximum extent, and even during transportation and storage, change of the escherichia coli content does not affect the total amount of the total coliform flora in the statistical sense; the preparation method of the sample is simple in process and high in successful rate.

The invention provides Enterobacter aerogenes M6R9 which can degrade pyrethroid pesticide residues, in particular can degrade the residues of three pyrethroid pesticides at the same time, namely bifenthrin, fenpropathrin and cypermethrin. The strain is preserved in the China General Microbiological Culture Collection Center (address: Institute of Microbiology of Chinese Academy of Sciences, Building 3, No. 1 Precincts, Beichen West Road, Chaoyang District, Beijing, 100101), the preservation number of the strain is CGMCC No. 4155, and the conservation date is September 8, 2010. The beneficial effects of the Enterobacter aerogenes M6R9 in the invention are mainly embodied as follows: the invention provides a new strain which can degrade the residues of bifenthrin, fenpropathrin and cypermethrin at the same time and provides the research basis for controlling the pyrethroid pesticide residues in agricultural products, and the strain has wide application prospect.

The invention provides a primer group for detecting escherichia coli O157 in feed. The primer group comprises an inner primer FIP, an inner primer BIP, an outer primer F3, an outer primer B3 and a loop primer LB. A method of detecting the escherichia coli O157 in the feed by using the primer group comprises the steps of escherichia coli O157 culture, DNA (deoxyribonucleic acid) extraction, loop-mediated isothermal amplification reaction and result observation. Tests show that the primer group has no specific expression for enterobacter aerogenes, escherichia coli and salmonella enteritidis, and obvious expression and high specificity for the escherichia coli O157; the feed is detected by a loop-mediated isothermal amplification detection method; a detection result is very precise; and a detection limit of the escherichia coli O157 in the feed is 2 CFU (colony forming unit)/25g. In addition, the primer group has the advantages of high detection speed, convenience in operation and the like for the detection of the feed.

The invention provides a microbial agent combining enterobacter aerogenes and klebsiella aerogenes, the microbial agent is used for treating lead and cadmium polluted soil, and the microbial agent comprises enterobacter aerogenes and klebsiella aerogenes; the enterobacter aerogenes is Enterobacter aerogenes W6, the Latin classification name of the enterobacter aerogenes is Enterobacter aerogenes, the enterobacter aerogenes is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is July 13, 2021, and the preservation number is CGMCC No.22888. The enterobacter aerogenes can be used for preparing the feed additive, and the feed additive can be used for preparing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive. The Klebsiella aerogenes are Klebsiella aerogenes Wn, the classification name of the Klebsiella aerogenes is Klebsiella aerogenes Wn, the Klebsiella aerogenes Wn is the Klebsiella aerogenes Wn, the Klebsiella aerogenes Wn is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is July 13, 2021, and the preservation number is CGMCC No. 22889.