Fungicide for producing biogas through fermentation of blue-green algae and bottom mud and application of fungicide

Abstract

The invention relates to the technical field of biomass fermentation, in particular to a microbial agent for producing biogas through fermentation of blue-green algae and bottom mud and application of the microbial agent, and is technically characterized in that the microbial agent is a composite microbial agent, and the microbial agent is formed by compounding enterobacter aerogenes, clostridium butyricum, bacillus amyloliquefaciens and mucor according to a proportion; the mucor genus belongs to circinellacoroides, and the mucor genus belongs to circinellacoroides; the circinellacoroides belongs to circinellacoroides; the preservation number of the enterobacter aerogenes is CGMCC (China General Microbiological Culture Collection Center) 1.10716; the preservation number of the clostridium butyricum is CGMCC (China General Microbiological Culture Collection Center) 1.5205; the preservation number of the bacillus amyloliquefaciens is CGMCC (China General Microbiological Culture Collection Center) 1.857; the preservation number of the rollernum mucoratum is CGMCC (China General Microbiological Culture Collection Center) 3.14110. When the microbial agent is used in the anaerobic reaction process of mixed fermentation of blue-green algae and bottom mud, the microbial compound bacteria are added, and the hydrolysis efficiency and biogas production efficiency of raw materials in the fermentation process can be effectively improved.

Description

Technical Field

[0001] The present invention relates to the technical field of biomass fermentation, and in particular to a bacterial agent for producing biogas by fermenting cyanobacteria and bottom mud and its application. Background Art

[0002] The removal of cyanobacteria and the harvesting of aquatic plants are crucial components of ecological and environmental conservation efforts. Furthermore, improper disposal of harvested aquatic plants (such as reed straw) can cause secondary pollution and waste resources. Therefore, the proper handling of these biomass wastes is crucial for environmental protection and resource recycling.

[0003] The traditional method for anaerobic fermentation of biomass waste is to directly ferment the biomass waste at room temperature, which has a very low hydrogen production efficiency or even no hydrogen production. In the prior art, a single bacterial species is used to perform anaerobic fermentation on biomass waste for a specific type of biomass, such as Bacillus subtilis for pretreatment of lignocellulosic biomass and Clostridium pasteurianum for anaerobic fermentation of agricultural waste (such as straw). However, the environmental adaptability of the single bacterial species used in the prior art is easily affected, and the degradation efficiency is low.

[0004] To this end, the present application provides a bacterial agent for producing biogas by fermenting cyanobacteria and bottom mud and its application to solve the above problems. Summary of the Invention

[0005] The purpose of the present invention is to solve the technical problems raised in the above-mentioned background technology and provide a bacterial agent for producing biogas by fermenting cyanobacteria and sediment and its application. When the bacterial agent of the present invention is used in the anaerobic reaction process of mixed fermentation of cyanobacteria and sediment, the addition of the microbial composite bacteria can effectively improve the hydrolysis efficiency of the raw materials and the biogas production efficiency during the fermentation process.

[0006] The above-mentioned purpose of the present invention is achieved like this:

[0007] In one aspect, the present invention provides a bacterial agent for producing biogas by fermenting cyanobacteria with sediment, wherein the bacterial agent is a composite bacterial agent composed of Enterobacter aerogenes, Clostridium butyricum, Bacillus amyloliquefaciens, and Mucor in a certain proportion; the Mucor is Circinella mucoroides.

[0008] The deposit number of the Enterobacter aerogenes is: CGMCC 1.10716;

[0009] The deposit number of the Clostridium butyricum is: CGMCC 1.5205;

[0010] The deposit number of the Bacillus amyloliquefaciens is: CGMCC 1.857;

[0011] The deposit number of the mucorformis is CGMCC 3.14110.

[0012] Furthermore, the percentages of Enterobacter aerogenes, Clostridium butyricum, Bacillus amyloliquefaciens and Mucor in the total number of bacterial cells in the composite bacterial agent are: 15% Enterobacter aerogenes, 25% Clostridium butyricum, 25% Bacillus amyloliquefaciens, 35% Mucor palustris.

[0013] The present invention also provides a bacterial agent for producing biogas by fermenting cyanobacteria and bottom mud, which is used as a fermentation bacterial agent in an anaerobic reaction process of mixed fermentation of cyanobacteria and bottom mud.

[0014] Furthermore, the dry weight ratio of cyanobacteria to sediment is 5:1.

[0015] Furthermore, the moisture content of the mixed fermentation substrate is controlled at 60%, the C / N ratio is between 25-30:1, and an inorganic nitrogen source is added for balancing; the pH value of anaerobic fermentation in the mixed fermentation is 6.8-7.2; the temperature of anaerobic fermentation is 25-30°C; the time of anaerobic fermentation is 6-8 days; and the stirring intensity is 60r / min.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] 1. Compared with the conventional method in the prior art of directly subjecting biomass waste to anaerobic fermentation at room temperature, the composite bacterial agent of the present invention can effectively improve the efficiency of straw hydrolysis and hydrogen production;

[0018] 2. The bacterial agent used in the present invention is a composite bacteria, each containing multiple hydrogen-producing enzymes, which fully utilizes the synergistic effect between strains to overcome the shortcomings of single strain fermentation and application, and has stronger environmental adaptability;

[0019] 3. The solution of the present invention not only solves the problem of recycling biomass waste such as straw, but also has important significance for promoting sustainable agricultural development and protecting the ecological environment, and has practical economic and social benefits and broad application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 is the daily hydrogen production in the hydrogen production reactor in the embodiment of the present invention;

[0021] Figure 2 is the percentage of methane content in the embodiment of the present invention. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0023] The implementation of the present invention is described in detail below with reference to specific embodiments.

[0024] Enterobacter aerogenes, Clostridium butyricum, Bacillus amyloliquefaciens and Circinella mucoroides in the examples of the present invention were purchased.

[0025] Reference Figure 1-Figure 2 The figure shows a preferred embodiment of the present invention.

[0026] Example: This embodiment of the present invention provides a bacterial inoculum for biogas production from cyanobacteria and sediment fermentation. This inoculum, when applied to anaerobic fermentation of cyanobacteria and sediment, can effectively improve the hydrolysis efficiency of the raw materials and biogas production during the fermentation process. The bacterial inoculum provided in this embodiment of the present invention is composed of the following four strains:

[0027] ① Enterobacter aerogenes, CGMCC 1.10716;

[0028] ②Clostridium butyricum, CGMCC 1.5205;

[0029] ③ Bacillus amyloliquefaciens, CGMCC 1.857;

[0030] ④Circinellamucoroides, CGMCC 3.14110.

[0031] The percentages of the bacterial cell numbers of the above four strains to the total bacterial cell numbers in the microbial composite bacteria are: Enterobacter aerogenes accounts for 15%, Clostridium butyricum accounts for 25%, Bacillus amyloliquefaciens accounts for 25%, and Mucor palustris accounts for 35%.

[0032] During the anaerobic mixed fermentation process of this embodiment, the dry weight ratio of cyanobacteria to sediment is 5:1. The moisture content of the mixed fermentation substrate is controlled at approximately 60%, and the C / N ratio is controlled at 25-30:1. Inorganic nitrogen sources, such as urea and ammonium salts, are primarily added for balancing. The pH of the anaerobic fermentation is 6.8-7.2. The temperature of the anaerobic fermentation is 25±1°C. The fermentation duration is 6-8 days. The agitation intensity is 60 rpm.

[0033] In this example, the bacterial strain was expanded using a growth medium comprising the following components: CH₃COONa at a concentration of 0.3 g / L, NH₄Cl at a concentration of 0.1 g / L, K₂HPO₄ at a concentration of 0.02 g / L, NaHCO₃ at a concentration of 0.2 g / L, NaCl at a concentration of 0.2 g / L, MgSO₄·7H₂O at a concentration of 0.02 g / L, and yeast extract at a concentration of 0.1 g / L. The medium was sterilized at 0.1 MPa for 20 min.

[0034] The bacteria of the bacterial agent of the present invention are expanded and cultured in a growth medium, and then mixed to expand the total number of bacteria to 10 10 The final inoculum volume was 5%.

[0035] The bacterial agent of this embodiment was used for anaerobic fermentation. The biogas production reactor was set up as follows: 200 mL of a mixture of cyanobacteria and bottom mud was added to a 300 mL wide-mouth bottle. After cooling, 10 mL (inoculation volume 5%) of the bacterial strain was inoculated. The C / N ratio in the fermentation broth was adjusted to 4, and the initial pH of the fermentation broth was adjusted to 7.0. Argon was filled into the headspace, and the reactor was sealed with a rubber stopper and paraffin wax. The culture temperature was 25°C. The gas produced by the fermentation was collected by the drainage gas collection method, and the gas production was recorded, as shown in Figure 2. Figure 1 As shown, it is the daily hydrogen production in the hydrogen production reactor; Figure 2 is the percentage of methane content.

[0036] Generally speaking, when the inoculant uses cyanobacteria and sediment as raw materials to produce biogas, the main biogas production stage is concentrated in the middle stage (4-16 days), and the specific gas production rate and specific methane production rate of cyanobacteria are the largest, which are 0.376 and 0.268 L / (ml·d), respectively.

[0037] Compared with the blank control group before adding bacteria, the results are shown in Table 1 below. The methane content increased by 4.3% and the biogas production rate increased by 0.168 L / (ml·d).

[0038] Table 1

[0039]

[0040] On the basis of the above embodiment, the proportion of bacterial strains was changed to obtain the control group as shown in Table 2 below:

[0041] Table 2

[0042]

[0043]

[0044] The gas production rate results of the control group are shown in Table 3 below:

[0045] Table 3

[0046]

[0047] In summary, through the above embodiments of the present invention, compared with the prior art, first, under normal temperature conditions, the hydrogen production efficiency of biomass waste directly used for anaerobic fermentation is very low or even no gas is produced. The solution of the present invention effectively improves the straw hydrolysis efficiency and hydrogen production by adding microbial composite bacteria. Secondly, the bacterial agent used in the present invention is a composite bacteria, which contains a variety of hydrogen-producing enzymes, and can make full use of the synergistic effect between strains to overcome the shortcomings of single strain fermentation and application, and has stronger environmental adaptability. Therefore, the implementation of the present invention can not only solve the problem of straw reuse, but also has important significance for promoting sustainable agricultural development and protecting the ecological environment, and has practical economic and social benefits and broad application prospects.

[0048] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A bacterial agent for producing biogas by fermenting cyanobacteria and sediment, characterized in that: The bacterial agent is a composite bacterial agent, which is composed of Enterobacter aerogenes, Clostridium butyricum, Bacillus amyloliquefaciens and Mucor in a certain proportion; the Mucor is Circinella mucoroides; The deposit number of the Enterobacter aerogenes is: CGMCC 1.10716; The deposit number of the Clostridium butyricum is: CGMCC 1.5205; The deposit number of the Bacillus amyloliquefaciens is: CGMCC 1.857; The deposit number of the mucorformis is CGMCC 3.14110.

2. The bacterial agent for producing biogas by fermentation of cyanobacteria and bottom mud according to claim 1, characterized in that: The percentages of Enterobacter aerogenes, Clostridium butyricum, Bacillus amyloliquefaciens and Mucor to the total number of bacterial cells in the composite bacterial agent are respectively: 15% Enterobacter aerogenes, 25% Clostridium butyricum, 25% Bacillus amyloliquefaciens and 35% Mucor palustris.

3. Use of the bacterial agent for producing biogas by fermentation of cyanobacteria and sediment according to claims 1-2 as a fermentation bacterial agent in an anaerobic reaction process of mixed fermentation of cyanobacteria and sediment.

4. The use according to claim 3, characterized in that The dry weight ratio of cyanobacteria to sediment is 5:

1.

5. The use according to claim 3, characterized in that The moisture content of the mixed fermentation substrate is controlled at 60%, the C / N ratio is between 25-30:1, and an inorganic nitrogen source is added for balance; the pH value of anaerobic fermentation in the mixed fermentation is 6.8-7.2; the temperature of anaerobic fermentation is 25-30°C; the time of anaerobic fermentation is 6-8 days; and the stirring intensity is 60r / min.

Images