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Raccoon dog distemper freeze-dried live vaccine and preparation method and application thereof

A raccoon canine distemper and vaccine technology, applied in the field of veterinary biological products, can solve the problems of inability to obtain better immune protection effect and lack of special treatment methods

Active Publication Date: 2017-09-15
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because raccoon distemper has the characteristics of acute onset and lack of effective treatment methods, vaccination is still the main prevention and control method for raccoon distemper. However, there is no raccoon distemper vaccine on the market at present. The canine distemper vaccine of other animals is used to prevent raccoon canine distemper, but it cannot achieve better immune protection effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 Domestication and Identification of Canine Distemper Virus Vaccine Strain CDV-O2

[0020] 1. Domestication of canine distemper virus vaccine strain CDV-O2

[0021] (1) Inoculate the preserved canine distemper virus vaccine strain CDV-O2 on African green monkey kidney cells (Vero) by adsorption and inoculation to carry out virus amplification and culture, observe the cell virus situation every day, and wait for the cytopathic changes to reach 80 % or so, the virus liquid was harvested by the method of repeated freezing and thawing, and the virus content (TCID 50 ≥10 5.0 / ml), sterility test. Aliquot the tested virus liquid and store it at -20°C.

[0022] (2) The amplified CDV-O2 virus liquid stock solution was inoculated into the hindlimb muscles of 3 antibody-negative healthy raccoon dogs, 5ml each, inoculated at three points, kept in isolation, and observed the physiological indicators of the inoculated raccoon dogs every day. On the 3rd and 5th day af...

Embodiment 2

[0029] Preparation of embodiment 2 raccoon dog distemper freeze-dried live vaccine (CDV-OR12 strain)

[0030] 1. Preparation of Canine Distemper Virus Seedling Solution

[0031] Take the preserved canine distemper virus attenuated vaccine strain CDV-OR12 and inoculate it on Vero cells by adsorption and inoculation for virus amplification and culture. Observe the cell virus situation every day. Methods The virus liquid was harvested and tested for virus content and purity. The content of raccoon canine distemper virus attenuated vaccine strain in the obtained virus liquid was TCID 50 ≥10 4.5 / ml. Store the qualified virus liquid at -20°C.

[0032] 2. Preparation of Lyoprotectant

[0033] Dissolve gelatin, hydrolyzed milk protein and sucrose in water, according to the mass percentage, so that the final concentration of gelatin is 5%, the final concentration of hydrolyzed milk protein is 15%, and the final concentration of sucrose is 25%. obtained after bacteria.

[0034] 3...

Embodiment 3

[0038] Safety test of embodiment 3 raccoon dog distemper freeze-dried live vaccine (CDV-OR12 strain)

[0039] Get the qualified raccoon dog distemper freeze-dried live vaccine (prepared in embodiment 2), hindlimb muscle inoculation antibody negative raccoon dog, establish single dose (vaccinate 1 portion, canine distemper virus content TCID 50 =10 4.5 / head), single-dose repeated and super-dose (10 times) immunization groups, and non-vaccinated raccoon dogs were set as negative controls. After vaccination, the body temperature, appetite, mental state, feces and other physiological indicators of raccoon dogs were observed for 14 consecutive days, and the lesions of the main organs of vaccinated raccoon dogs were observed by autopsy. The test results showed that after single-dose, repeated single-dose and over-dose inoculation, all physiological indexes of raccoon dogs were normal without any clinical symptoms, and no pathological changes were observed in the main organs of rac...

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Abstract

The invention discloses a raccoon dog distemper freeze-dried live vaccine and a preparation method and application thereof and belongs to the technical field of biological products for animals. The effective component of the raccoon dog distemper freeze-dried live vaccine is a raccoon dog distemper virus attenuated vaccine strain. The attenuated vaccine strain is a dog distemper attenuated vaccine strain which is obtained through acclimatization on a dog distemper vaccine strain CDV-O2, and is safe to a raccoon dog, good in immunogenicity and stable in titer, the attenuated vaccine strain is named as CDV-OR12 strain, and the microbial preservation number of the attenuated vaccine strain is CGMCC NO. 13793. Experiments verify that the vaccine can effectively prevent the raccoon dog from being attacked by dog distemper virus virulent viruses, the protection rate is more than 90 percent, raccoon dog distemper can be effectively prevented, and the immunity period is 6 months. A new technical way for preventing and controlling raccoon dog distemper is provided.

Description

technical field [0001] The invention relates to a freeze-dried live vaccine for raccoon canine distemper and its preparation method and application, and belongs to the technical field of veterinary biological products. Background technique [0002] Canine distemper is an acute, febrile, and highly contagious infectious disease caused by canine distemper virus of the genus Morbillivirus in the Paramyxoviridae family. Most of the young animals have an acute and fatal course, and adult animals can become chronic and persistent. Infection, the clinical symptoms are mainly characterized by biphasic elevated body temperature, accompanied by catarrhal pneumonia and gastroenteritis, and occasionally neurological symptoms appear, which is prone to secondary mixed infection and secondary infection of other bacteria and viruses, and the mortality rate can be as high as 80% %, is one of the most serious diseases that endanger my country's fur animal breeding industry. [0003] Raccoon ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/175A61K9/19A61K47/42A61K47/26A61P31/14C12N7/04
CPCA61K9/19A61K39/12A61K47/26A61K47/42A61K2039/5254A61K2039/552C12N7/00C12N2760/18421C12N2760/18434
Inventor 胡博闫喜军王洋吕爽廉士珍刘昊张蕾白雪鲁荣光张海玲赵建军朱言柱史宁薛向红邓效宇徐淑娟
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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