9results about How to "Improve immune activity" patented technology

The invention relates to a respiratory syncytial virus vaccine composition and application thereof. The recombinant respiratory syncytial virus vaccine comprises RSVpreF protein and a pharmaceutically acceptable composite adjuvant carrier, and the composite adjuvant carrier is formed by combining an aluminum adjuvant with a CpG adjuvant. Experimental results prove that the vaccine composition provided by the invention has good immunogenicity and a better immune effect.

The invention provides a liposome adjuvant as well as a preparation method and application thereof, and belongs to the technical field of biomedical engineering. A liposome adjuvant system comprises an aqueous phase and a lipid component, the aqueous phase comprising a water-soluble adjuvant present in the internal aqueous phase of the liposome and an aqueous phase medium, the aqueous phase medium comprising from 1% w / v to 15% w / v of a sugar and / or a sugar alcohol. The liposome adjuvant system provided by the invention has higher encapsulation efficiency, stability and immunocompetence, and meanwhile, the influence of common buffer solution, sodium chloride and other salt components on the function of the liposome adjuvant is avoided. In addition, according to the liposome adjuvant system, the cell toxicity and the in-vivo toxicity of the liposome adjuvant system can be reduced by reducing the dosage of cationic lipids such as DOTAP and DOTMA.

This invention discloses an extracellular polysaccharide DSP-1 from the shell of a deep-sea fungus, *Subseptospora*, and its applications. Didymella keratinophila The fermentation product of SCAU273 was isolated and purified to obtain an extracellular polysaccharide DSP-1. This polysaccharide has a molecular weight of 4116 Da, a bibranched structure, and is mainly composed of glucose polymers. Further studies showed that DSP-1 can enhance the activity and promote the proliferation of RAW 264.7 macrophages over a wide concentration range (25–400 μg / mL). Furthermore, at certain concentrations, it dose-dependently promoted the secretion of NO, TNF-α, IL-6, and IL-1β by RAW 264.7 macrophages, exhibiting good immunomodulatory activity against RAW 264.7 macrophages. It shows broad prospects for the production and widespread application of environmentally friendly marine biological agents.

The invention relates to a hirsutella sinensis strain as well as a fermentation method and application thereof, and belongs to the technical field of microorganisms. The strain is separated from a natural cordyceps sinensis sample, and green, high-yield, stable and sustainable Hirsutella sinensis mycelia are obtained through a liquid fermentation process. The strain disclosed by the invention shows excellent growth and proliferation rates in fermentation culture, and the adenosine content of the strain is surprisingly high. In addition, the strain disclosed by the invention shows an excellent bidirectional immunoregulation effect, not only can effectively improve the immune function of macrophages in a resting state, but also has an inhibition effect on excessively activated macrophages.

The invention discloses a botanical organic selenium cat food with an immune regulation function. According to the cat food, yeast beta-glucan, lactoferrin and plant source organic selenium are added on the basis of basic cat food, the addition amount of the yeast beta-glucan is 300-500 mg / kg, the addition amount of the lactoferrin is 100-150 mg / kg, the total selenium content in the cat food is 0.4-0.5 mg / kg, and the organic selenium accounts for 80-90% of the total selenium content. The plant source organic selenium exists in a form of combining seleno-amino acid with protein. Through the synergistic compatibility of various immune function factors, the immune regulation function of the cat food is improved on the premise of ensuring the safety and the stability, and the cat food is suitable for the field of functional pet food.

The application discloses a preparation process of bifidobacterium live bacteria for regulating immunity and application thereof. Animal bifidobacterium (Bifidobacterium animalis), short bifidobacterium (Bifidobacterium breve) and long bifidobacterium (Bifidobacterium longum) are used as production strains, and a five-in-one preparation process is adopted, that is, a solid fermentation oat nutrient medium preparation process using yeast, a starvation stress-supplement recovery secondary anaerobic fermentation process, a low-temperature high-speed centrifugal lossless concentration process, a composite anti-reverse protective agent emulsification process and a gradient temperature freeze-drying process, so that high-activity bifidobacterium live bacteria preparation with a live bacteria number of greater than or equal to 2.0*10 11 CFU / g, a water activity of less than or equal to 0.22, a gastric acid survival rate of greater than or equal to 85%, and a survival rate of greater than or equal to 85% after being accelerated at 37 DEG C for three months is finally obtained, and the bifidobacterium colonization capacity, the immunoregulation activity, the intestinal tolerance capacity and the long-term storage stability are significantly improved.

The application discloses a method for quantifying and distinguishing MSC immunological activity by CD82 and application, collects cells, uses a fluorescently labeled antibody to mark a membrane protein CD82 on a surface of MSC, and uses a flow cytometer to detect a positive rate and MFI of the cell CD82, quantifies the MSC immunological activity by an expression level of the CD82, the immunological activity refers to an inhibition ability of the MSC on secretion of inflammatory cytokines TNF-alpha and IFN-gamma by activated PBMC, meanwhile, the MSC cell group with high immunological activity can be distinguished by detecting the expression level of the CD82 on the MSC, the MSC cell group with high immunological activity screened by using the method shows good curative effect in treating diseases related to inflammation and immunity, the detection method is simple, easy to operate, economical and efficient, and has wide application prospect in a stem cell drug market.

The application provides a SAA recombinant protein and a preparation method and application thereof, and the preparation method comprises the following steps: S10, constructing a recombinant plasmid: a gene sequence of an amino acid sequence shown in SEQ ID NO: 1 or a nucleotide sequence corresponding to an amino acid sequence shown in SEQ ID NO: 2 is inserted into a pET-28a(+) plasmid which is double-digested by NdeI and XhoI after being double-digested by NdeI and XhoI, so that a recombinant plasmid is obtained; S20, transforming and culturing the recombinant plasmid; S30, protein expression and purification. The application solves the technical problem that the stability of the currently recombinantly expressed SAA protein in vitro is poor, and achieves the technical effect of improving the stability of the SAA recombinant protein in vitro.

The invention relates to the technical field of feed additives, in particular to a rhizoma atractylodis macrocephalae polysaccharide extract, a preparation method of the rhizoma atractylodis macrocephalae polysaccharide extract, a compound feed additive for piglets and a feed for the piglets. The preparation method of the atractylodes macrocephala polysaccharide extract comprises the following steps: sequentially carrying out reflux degreasing, ultrasonic treatment and compound enzyme enzymolysis treatment on atractylodes macrocephala rhizomes. According to the method, fat-soluble components can be removed in advance, and a basis is provided for extraction and purification of polysaccharide components; then, cell wall structures are destroyed through ultrasonic treatment, so that more contact areas exist between the materials and the solvent, more enzymolysis sites are exposed, and more polysaccharide components are released. Furthermore, the atractylodes macrocephala koidz polysaccharide obtained by the method has relatively high active ingredients, and is in synergistic compatibility with components such as pachymaran and ellagic acid, so that the immunity of the piglets is improved, and the diarrhea rate is reduced.