29results about How to "Improve immune activity" patented technology

The invention belongs to the technical field of food processing, and provides a spina date seed peptide zinc chelate as well as a preparation method and application thereof. The spina date seed peptide zinc chelate is stable in performance and high in absorption efficiency. The preparation method comprises the following steps: degreasing a spina date seed medicinal material; preparing spina date seed protein; preparing spina date seed polypeptide; carrying out chelation reaction on the spina date seed polypeptide and zinc sulfate heptahydrate; and separating a chelation reaction product. The spina date seed peptide zinc chelate prepared by the invention can be directly used as a natural immunopotentiator to improve the immunity of a human body. The method has the advantages of short production cycle, low cost, no pollution to the environment, abundant nutrition, high protein content, small particle size, high safety and high utilization rate of effective components, and can be widely applied to the fields of foods, medicines and health care.

The invention provides a methylobacterium strain JC03 for producing single-cell protein and application of the methylobacterium strain JC03 in the field of aquaculture, and belongs to the technical field of microorganisms. The methylobacterium strain JC03 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.32616. The strain is high in growth efficiency, high in thallus crude protein content, rich in amino acid spectrum, high in essential amino acid content and rich in functional components for improving the immunocompetence of aquatic animals; the single-cell protein produced by the strain can completely or partially replace fish meal protein in feeds of marine fishes such as turbot, pseudosciaena crocea, grouper and rainbow trout; vegetable protein in feed of freshwater fish such as grass carp can be completely replaced; animal and plant proteins in feeds for shrimps and crabs, such as penaeus vannamei, can be completely replaced, and animal and plant proteins in stichopus japonicus feeds can be completely replaced; the immunocompetence of fed aquatic animals can be obviously improved. The invention provides a microbial strain for efficiently converting methanol to produce single-cell protein, and the microbial strain has a wide application prospect in the field of aquaculture.

The invention provides a preparation method of DC cells. The preparation method is especially suitable for rapidly obtaining high-purity and high-functional-activity DC cells. The method comprises the steps of separation from peripheral blood mononuclear cells (PBMC), induction culture, maturation treatment and the like, maturation of the dendritic cells is promoted by introducing an anti-CD47 single-domain antibody or LPS, and the immunocompetence of the dendritic cells is remarkably improved. The method is further applied to preparation of an anti-tumor cell preparation, the capability of activating tumor specific T cells by tumor antigen-loaded DC cells can be remarkably enhanced, and the killing activity of the T cells to tumor cells is improved. The preparation method provided by the invention overcomes the defects in the prior art, has the advantages of high efficiency, safety and stability, and can provide a more reliable cell therapy scheme for tumor immunotherapy.

The invention discloses a foot and mouth disease virus effective antigen inactivation protective agent and application thereof. The protective agent is prepared from the following components in percentage by mass: 0.05 to 20 percent of a structure curing agent, 0.01 to 5 percent of an ion regulator, 0.005 to 1.0 percent of an antioxidant and the balance of water, the protective agent does not influence the inactivation effect in the process of acting on nucleic acid, inhibits folding and unfolding of a protein physical structure, prevents change of a protein space physical structure, improves free energy of protein denaturation, enhances the stability of a virus space physical structure, maintains the integrity of virions, and has a good application prospect. The effects of preserving the immune biological activity of the protein and reducing the loss of viruses in the inactivation process are achieved, so that the foot-and-mouth disease inactivation antigen liquid with high immunogenicity is obtained. Compared with the conventional antigen protection method, the method has the advantages that the virus is protected before the inactivation process is implemented, the production process is advanced, the stability of the effective antigen can be protected to the greatest extent, and support is provided for obtaining an effective antigen solution with high immunocompetence.

The invention relates to galactomannan tetrasaccharide with anti-tumor activity and application of the galactomannan tetrasaccharide, and belongs to the technical field of active oligosaccharide drugs. The galactomanninotetraose ACP-3 synthesized by the invention has a good anti-tumor effect, is effective and safe, and has a very good development value. The galactomanninotetraose ACP-3 can obviously inhibit tumor cell proliferation in vitro and has no obvious toxicity to normal cells, so that the antitumor effect of the galactomanninotetraose ACP-3 has selectivity. In-vivo anti-tumor experiments show that the immunologic function of a body can be improved and tumor growth can be inhibited by single administration of the galactomanninotetraose ACP-3, and the galactomanninotetraose ACP-3 and CTX or PTX are combined for use, so that the effects of enhancing effects and reducing toxicity are achieved. The invention is helpful for developing oligosaccharide drugs with anti-tumor effect, and has important significance for research and development of corresponding carbohydrate drugs.

The invention belongs to the technical field of biology, and particularly relates to application of an ags1 gene segment in regulating monascus exopolysaccharide synthesis. According to the invention, the ags1 gene segment related to synthesis of a mycelium aggregation factor-cell wall alpha-1, 3-glucan is found from monascus for the first time, and experimental research finds that compared with an initial strain, the content of exopolysaccharides in liquid fermentation liquor of monascus recombinant bacteria of which the gene segment is knocked out is remarkably increased, and the content of the exopolysaccharides in the liquid fermentation liquor is remarkably reduced. The content of extracellular polysaccharide in liquid fermentation liquor of monascus recombinant bacteria overexpressing the gene segment is reduced, but the relative content of galactomannan in the extracellular polysaccharide is increased, so that the monascus recombinant bacteria have higher immunocompetence.

The invention discloses Longshan lily bulbil polysaccharide as well as a preparation method and application thereof, and belongs to the technical field of plant extraction. In the polysaccharide, the total sugar content is greater than 94%, monosaccharide comprises mannose, rhamnose, glucose, galactose and galacturonic acid in a ratio of (5.12 to 5.48): (7.04 to 7.79): (61.55 to 62.38): (17.37 to 18.14): (7.28 to 7.91), and the weight-average molecular weight is 183916 to 184735 Da. The preparation method comprises the following steps: by taking Lilium brownii Bulb as a raw material, carrying out boiling water enzyme deactivation treatment, decolorizing and degreasing, extracting an alcohol insoluble substance with water, synchronously removing starch by using high-temperature-resistant amylase, carrying out microwave digestion on filter residues obtained after filtering by using a sodium hydroxide solution, dialyzing and extracting an extracting solution, and finally preparing the Lilium brownii Bulb polysaccharide with immunocompetence. The method can be applied to food or functional food or medicine, and provides a thought for high-value application of the Longshan lily.

The invention provides a safe and effective selenium supplement, namely a cabbage mustard seedling selenium-carrying system and a preparation method thereof. The invention also provides application of the selenium-loaded system of the cabbage mustard in an anti-tumor immunomodulator, the selenium-loaded system of the cabbage mustard has excellent characteristics of the cabbage mustard and selenium, and the excellent pharmacological activity of the selenium-loaded system is enhanced under the action of the selenium. The compound provided by the invention has good NK92 cell enhancement effect and tumor sensitization effect, has good sensitization effect on anti-tumor immunoregulation of NK92 cells, has small adverse reaction, and provides a new scientific basis for developing a safe and efficient immunomodulator.

The invention discloses a combined water-soluble fertilizer containing plant immune activator protein and seaweed and a preparation method, and relates to the technical field of chemical fertilizer preparation, the combined water-soluble fertilizer comprises the following components by mass: 50-70 parts of a seaweed mother liquor, 20-30 parts of a plant immune activator protein mother liquor (containing a protein stabilizer), and 5-15 parts of an organic matter, the organic matter comprises one or more compositions of glucose, glycerol or sugar alcohols. The natural organic seaweed fertilizer and the plant immune activator protein are reasonably proportioned, and the functions and characteristics of the natural organic seaweed fertilizer and the plant immune activator protein are fused together, so that on one hand, organic raw materials provided by the seaweed can promote the plant immune activator protein to better exert the immune activity of the plant immune activator protein; on the other hand, the plant immune activator protein can actively mobilize the absorption and utilization of the seaweed fertilizer by plants, and the purposes of reducing the use of the fertilizer, increasing the yield and improving the quality of crops are achieved.

The invention relates to the technical field of tilapia mossambica culture, in particular to a tilapia mossambica culture system capable of controlling harmful algal blooms. The system comprises a tilapia mossambica culture system, a graded filtering system, a biological fluidized bed system and an aquatic plant planting system which are sequentially connected through water pipes; the tilapia mossambica culture system comprises a culture pond, the aquatic plant planting system comprises an aquatic plant planting pond, and aquatic economic plants are planted in the aquatic plant planting pond. The effect of purifying the tilapia mossambica culture water environment can be achieved, tilapia mossambica is prevented from gnawing plant roots through ectopic planting of aquatic plants, harmful algal blooms such as blue-green algae are effectively inhibited through rhizosphere secretions, the immunocompetence of tilapia mossambica is improved, tilapia mossambica health is promoted, and the tilapia mossambica culture yield is increased; meanwhile, the earthy smell generated after peculiar-smell substances such as blue-green algae are eaten by the tilapia is reduced.

The invention belongs to the technical field of functional food, and particularly relates to a composition containing anti-helicobacter pylori egg yolk globulin and curcumin and application of the composition in anti-helicobacter pylori functional food. The composition is prepared from the following raw materials in parts by weight: 0.1 to 0.3 part of curcumin, 0.01 to 0.03 part of allicin, 0.01 to 0.05 part of ginsenoside Ro, 1 to 3 parts of a biomass extract, 1 to 5 parts of egg yolk globulin powder, 1 to 3 parts of a modified oligosaccharide mixture and 0.1 to 0.5 part of probiotics. The composition can inhibit helicobacter pylori infection and relieve stomach discomfort.

The invention relates to a probiotic and a feed additive for fish culture and application of the probiotic and the feed additive, and the probiotic for fish culture is a Lactococcus lactis strain HNL12 (MK889240) separated from intestinal tracts of wild humpback perches by a research group. The probiotic feed additive disclosed by the invention is used for promoting the growth of the humpback perches, improving the non-specific immunity of the humpback perches and improving the antibacterial ability of the humpback perches. The method is of great significance to healthy breeding of the humpback perches and prevention and control of bacterial diseases.

The invention relates to a respiratory syncytial virus vaccine composition and application thereof. The recombinant respiratory syncytial virus vaccine comprises RSVpreF protein and a pharmaceutically acceptable composite adjuvant carrier, and the composite adjuvant carrier is formed by combining an aluminum adjuvant with a CpG adjuvant. Experimental results prove that the vaccine composition provided by the invention has good immunogenicity and a better immune effect.

The invention provides a liposome adjuvant as well as a preparation method and application thereof, and belongs to the technical field of biomedical engineering. A liposome adjuvant system comprises an aqueous phase and a lipid component, the aqueous phase comprising a water-soluble adjuvant present in the internal aqueous phase of the liposome and an aqueous phase medium, the aqueous phase medium comprising from 1% w / v to 15% w / v of a sugar and / or a sugar alcohol. The liposome adjuvant system provided by the invention has higher encapsulation efficiency, stability and immunocompetence, and meanwhile, the influence of common buffer solution, sodium chloride and other salt components on the function of the liposome adjuvant is avoided. In addition, according to the liposome adjuvant system, the cell toxicity and the in-vivo toxicity of the liposome adjuvant system can be reduced by reducing the dosage of cationic lipids such as DOTAP and DOTMA.

This invention discloses an extracellular polysaccharide DSP-1 from the shell of a deep-sea fungus, *Subseptospora*, and its applications. Didymella keratinophila The fermentation product of SCAU273 was isolated and purified to obtain an extracellular polysaccharide DSP-1. This polysaccharide has a molecular weight of 4116 Da, a bibranched structure, and is mainly composed of glucose polymers. Further studies showed that DSP-1 can enhance the activity and promote the proliferation of RAW 264.7 macrophages over a wide concentration range (25–400 μg / mL). Furthermore, at certain concentrations, it dose-dependently promoted the secretion of NO, TNF-α, IL-6, and IL-1β by RAW 264.7 macrophages, exhibiting good immunomodulatory activity against RAW 264.7 macrophages. It shows broad prospects for the production and widespread application of environmentally friendly marine biological agents.

The invention relates to a hirsutella sinensis strain as well as a fermentation method and application thereof, and belongs to the technical field of microorganisms. The strain is separated from a natural cordyceps sinensis sample, and green, high-yield, stable and sustainable Hirsutella sinensis mycelia are obtained through a liquid fermentation process. The strain disclosed by the invention shows excellent growth and proliferation rates in fermentation culture, and the adenosine content of the strain is surprisingly high. In addition, the strain disclosed by the invention shows an excellent bidirectional immunoregulation effect, not only can effectively improve the immune function of macrophages in a resting state, but also has an inhibition effect on excessively activated macrophages.

The invention discloses a botanical organic selenium cat food with an immune regulation function. According to the cat food, yeast beta-glucan, lactoferrin and plant source organic selenium are added on the basis of basic cat food, the addition amount of the yeast beta-glucan is 300-500 mg / kg, the addition amount of the lactoferrin is 100-150 mg / kg, the total selenium content in the cat food is 0.4-0.5 mg / kg, and the organic selenium accounts for 80-90% of the total selenium content. The plant source organic selenium exists in a form of combining seleno-amino acid with protein. Through the synergistic compatibility of various immune function factors, the immune regulation function of the cat food is improved on the premise of ensuring the safety and the stability, and the cat food is suitable for the field of functional pet food.

The application discloses a preparation process of bifidobacterium live bacteria for regulating immunity and application thereof. Animal bifidobacterium (Bifidobacterium animalis), short bifidobacterium (Bifidobacterium breve) and long bifidobacterium (Bifidobacterium longum) are used as production strains, and a five-in-one preparation process is adopted, that is, a solid fermentation oat nutrient medium preparation process using yeast, a starvation stress-supplement recovery secondary anaerobic fermentation process, a low-temperature high-speed centrifugal lossless concentration process, a composite anti-reverse protective agent emulsification process and a gradient temperature freeze-drying process, so that high-activity bifidobacterium live bacteria preparation with a live bacteria number of greater than or equal to 2.0*10 11 CFU / g, a water activity of less than or equal to 0.22, a gastric acid survival rate of greater than or equal to 85%, and a survival rate of greater than or equal to 85% after being accelerated at 37 DEG C for three months is finally obtained, and the bifidobacterium colonization capacity, the immunoregulation activity, the intestinal tolerance capacity and the long-term storage stability are significantly improved.

The present invention relates to a grass carp feed additive and a preparation method and application thereof, wherein the feed additive comprises the following components: sucrose fatty acid ester, tributyrin, taurine, curcumin, astragalus polysaccharide, yeast cell wall, xylanase, bile acid salt, dandelion, phospholipid powder, magnesium and zinc. The several components selected for the grass carp feed additive of the present application play the best wintering protection effect under the synergistic effect, which is much stronger than the effect of using them alone, can prevent the occurrence of excessive consumption of nutrients, organ atrophy and decreased immunity of grass carp, effectively maintain the normal health of wintering grass carp, and avoid the occurrence of "wintering syndrome".

The invention relates to the technical field of polysaccharide medicinal preparations, and particularly discloses a fucoidin-containing composition for chronic nephritis, which comprises the following raw materials: fucoidin, Chinese herbal medicine extract and auxiliary materials. The fucoidin is prepared by the following specific operations: (1) airing and pulping fresh kelp, adding into a tartaric acid solution, grinding for 1-2 hours, adding sodium bicarbonate, stirring, performing electric field pulse treatment for 2-4 minutes in the stirring process, adding a calcium chloride solution, stirring, centrifuging, performing solid-liquid separation, adding trichloroacetic acid into liquid, stirring, centrifuging, and collecting supernate to obtain first supernate; (2) adding a first part of absolute ethyl alcohol into the first supernatant, stirring, standing, centrifuging, and collecting the supernatant to obtain a second supernatant; and adding a second part of absolute ethyl alcohol into the second supernate, stirring, standing, centrifuging, collecting lower-layer precipitate, drying, grinding, adding into deionized water, centrifuging for 1-2 hours, collecting precipitate, washing, and freeze-drying.

The application discloses a method for quantifying and distinguishing MSC immunological activity by CD82 and application, collects cells, uses a fluorescently labeled antibody to mark a membrane protein CD82 on a surface of MSC, and uses a flow cytometer to detect a positive rate and MFI of the cell CD82, quantifies the MSC immunological activity by an expression level of the CD82, the immunological activity refers to an inhibition ability of the MSC on secretion of inflammatory cytokines TNF-alpha and IFN-gamma by activated PBMC, meanwhile, the MSC cell group with high immunological activity can be distinguished by detecting the expression level of the CD82 on the MSC, the MSC cell group with high immunological activity screened by using the method shows good curative effect in treating diseases related to inflammation and immunity, the detection method is simple, easy to operate, economical and efficient, and has wide application prospect in a stem cell drug market.

The present invention belongs to the technical field of immune biological products, and specifically relates to a hairtail immune peptide FAFU-C for alleviating imidacloprid-induced immune damage and a preparation method thereof. The amino acid sequence of the hairtail immune peptide FAFU-C of the present invention is Asn-Gln-Val-Ala-Met-Asn-Pro-Thr-Asn-Thr-Val-Phe-Asp-Ala-Lys. When mice were intervened with imidacloprid, their immune function was damaged, indicating that imidacloprid has a certain feasibility in establishing an immune damage model mouse, and the hairtail immune peptide FAFU-C of the present invention can alleviate the immune damage of mice induced by imidacloprid.

The application provides a SAA recombinant protein and a preparation method and application thereof, and the preparation method comprises the following steps: S10, constructing a recombinant plasmid: a gene sequence of an amino acid sequence shown in SEQ ID NO: 1 or a nucleotide sequence corresponding to an amino acid sequence shown in SEQ ID NO: 2 is inserted into a pET-28a(+) plasmid which is double-digested by NdeI and XhoI after being double-digested by NdeI and XhoI, so that a recombinant plasmid is obtained; S20, transforming and culturing the recombinant plasmid; S30, protein expression and purification. The application solves the technical problem that the stability of the currently recombinantly expressed SAA protein in vitro is poor, and achieves the technical effect of improving the stability of the SAA recombinant protein in vitro.

The invention belongs to the technical field of preparation of probiotics and medicinal and edible raw materials, and particularly relates to a composition containing probiotics as well as a preparation method and application of the composition. The composition containing the probiotics is prepared from the following raw materials: 3 to 20 parts of fructus crataegi, 0.1 to 2 parts of endothelium corneum gigeriae galli, 0.3 to 3 parts of poria cocos, 0.5 to 5 parts of malt, 0.2 to 2 parts of lactobacillus rhamnosus GG, 0.2 to 2 parts of bifidobacterium animalis subsp. Lactis HN019, 0.02 to 1 part of lactobacillus fermentum CECT5716 and 0.02 to 1 part of lactobacillus rhamnosus HN001. The solid composition based on probiotics provided by the invention has the effects of regulating gastrointestinal functions, relaxing bowel and improving and promoting immunity. And the suspension which is fine, free of granular sensation and relatively uniform in dispersion can be formed only by brewing with warm water. The composition is not easy to cake in the transportation and storage processes, can maintain a relatively good number of living bacteria, and has a good application value.

The invention relates to the technical field of feed additives, in particular to a rhizoma atractylodis macrocephalae polysaccharide extract, a preparation method of the rhizoma atractylodis macrocephalae polysaccharide extract, a compound feed additive for piglets and a feed for the piglets. The preparation method of the atractylodes macrocephala polysaccharide extract comprises the following steps: sequentially carrying out reflux degreasing, ultrasonic treatment and compound enzyme enzymolysis treatment on atractylodes macrocephala rhizomes. According to the method, fat-soluble components can be removed in advance, and a basis is provided for extraction and purification of polysaccharide components; then, cell wall structures are destroyed through ultrasonic treatment, so that more contact areas exist between the materials and the solvent, more enzymolysis sites are exposed, and more polysaccharide components are released. Furthermore, the atractylodes macrocephala koidz polysaccharide obtained by the method has relatively high active ingredients, and is in synergistic compatibility with components such as pachymaran and ellagic acid, so that the immunity of the piglets is improved, and the diarrhea rate is reduced.

The invention belongs to the field of selenium polysaccharide application, and particularly relates to application of selenium polysaccharide in preparation of an immunocompetence agent.Cyclocarya paliurus is treated through exogenous selenium, and the obtained selenium polysaccharide has good immunocompetence, can better promote proliferation and phagocytic functions of RAW264.7 macrophages, has remarkable advantages in the aspect of increasing the NO release amount, and has good application prospects. The result shows that the polysaccharide strengthened by the exogenous selenium has better immunocompetence. Exogenous selenium strengthening obviously improves the characteristics of selenium polysaccharide in the cyclocarya paliurus leaves, has better potential in the aspects of oxidation resistance and immunity improvement, and provides data support for further research and utilization.

The invention provides a hemp seed active peptide with an immunological enhancement effect as well as a preparation method and application thereof, and belongs to the technical field of preparation of bioactive peptides. The amino acid sequence of the hemp seed protein source immunological enhancement active peptide provided by the invention is DGDGSGFF. The China-hemp seed protein source immunological enhancement active peptide provided by the invention is separated from China-hemp seed protein for the first time, is a novel China-hemp seed protein source immunological enhancement active peptide, has good immunological enhancement activity, can be used as a natural immunopotentiator in food, health care products and immunological enhancement drugs, and has good application prospects.

The present invention discloses a recombinant antigen protein TatB of Helicobacter pylori and a preparation method and application thereof. The amino acid sequence of the recombinant antigen protein TatB is shown in SEQ ID NO:1, and the nucleotide sequence of the recombinant antigen protein TatB encoding gene is shown in SEQ ID NO:2. The present invention obtains a high-purity antigen protein through the steps of plasmid construction, induced expression, affinity chromatography and exchange chromatography purification. The purification process is simple, the cost is low, and the operability is strong. The purified antigen has high immunogenicity and immune titer and can be used as a genetic engineering subunit vaccine. As an important component protein of the Helicobacter pylori membrane transport protein, the recombinant antigen protein TatB will become one of the most promising candidate antigens in the development of Helicobacter pylori genetic engineering vaccines.

The invention belongs to the technical field of extraction and preparation of natural products, and discloses camellia seed cake polysaccharide with immunological enhancement and antioxidant activity as well as a preparation method and application of the camellia seed cake polysaccharide. The method comprises the following steps: extracting camellia seed cake polysaccharide by adopting an ultrasonic-assisted method and a water extraction and alcohol precipitation method, determining the content of crude polysaccharide by adopting a phenol-sulfuric acid method, desalting, removing impurities, purifying and grading the camellia seed cake crude polysaccharide by adopting a DEAE-Sepharose FF ion exchange column and a Chromdex 75PG gel filter column to obtain camellia seed cake pure polysaccharide, and determining the molecular weight of the polysaccharide by adopting an exclusion chromatography method. And finally, determining the structure of the compound through chemical methods such as high performance liquid chromatography, full methylation combined gas chromatography-mass spectrometry, infrared spectroscopy, nuclear magnetic resonance and the like, a chromatographic method and a spectrum method. In-vitro experiments and in-vivo experiments prove that the camellia seed cake polysaccharide has immunological enhancement and antioxidant activity at the same time, and can be widely applied to the fields of medicines, daily chemical products, feeds and the like.