448results about How to "Improve immune activity" patented technology

Selective antagonists of A2A adenosine receptors are provided. The novel A2A blockers are useful for the treatment of Parkinson's disease and other diseases.

The invention relates to administration of an anti-CTLA-4 antibody, particularly human antibodies to human CTLA-4, such as those having amino acid sequences of antibodies 3.1.1, 4.1.1, 4.8.1, 4.10.2, 4.13.1, 4.14.3, 6.1.1, 11.2.1, 11.6.1, 11.7.1, 12.3.1.1, 12.9.1.1, and MDX-010, in combination with an immunostimulatory nucleotide, i.e, CpG ODN PF3512676, for treatment of cancer. The invention relates to administering a combination of an anti-CTLA-4 antibody and CpG ODN PF3512676 as neoadjuvant, adjuvant, first-line, second-line, and third-line therapy of cancer, whether localized or metastasized, and at any point(s) along the disease continuum (e.g, at any stage of the cancer).

The invention discloses an enteromorpha polysaccharide having immunocompetence and a preparation method thereof. The enteromorpha polysaccharide having immunocompetence is a polysaccharide HT-II. The enteromorpha polysaccharide having immunocompetence is a white floccule, can be well dissolved in water and dimethyl sulfoxide, has relative molecular weight of 96kDa, and mainly comprises glucose and mannose, wherein a ratio of rhamnoside to xylose to mannoside to glucose is 36: 20: 6: 38. The preparation method comprises the following steps of breaking down, carrying out extraction at a temperature of 90 DEG C by water, filtrating, merging the extract, concentrating, carrying out precipitation by ethanol, removing proteins, carrying out separation and purification, and carrying out freeze drying to obtain the purified enteromorpha polysaccharide having immunocompetence. The preparation method is simple, has stable performances, a low cost and a large medical value, can reduce cholesterol content and can improve immunity. The preparation method adopts extraction, protein removal and purification processes, and scientific experimental means, is a novel method for controlling enteromorpha, and provides a novel approach for controlling enteromorpha. The preparation method of the enteromorpha polysaccharide having immunocompetence develops enteromorpha medical values and purposes, controls environmental pollution, reduces a clean-up cost, improves economic benefits and is an ideal special preparation technology for enteromorpha control and integrated utilization.

The present invention discloses a recombinant baculovirus strain rBac / PCV2Cap (microorganism preservation number: CGMCC NO.2083) efficiently expressing Porcine circovirus type 2 Cap protein and applications thereof. The recombinant baculovirus strain rBac / PCV2Cap constructed by the present invention can efficiently express recombinant PCV2-Cap protein in insect cells, and the expressed recombinant Cap protein, which has good immunocompetence and antigenicity, can serve as a subunit vaccine used to prevent the related plague caused by Porcine circovirus type 2 infection as well as a detecting and diagnostic antigen for Porcine circovirus type 2 serum antibody.

The invention provides a composite active bacterial preparation which is used for aquatic products and has the water improving and fertilizing functions and a preparation method thereof by applying a modern bioengineering technology mainly for the problem that in existing aquaculture, the water quality of a pond is poor. The composite microecological preparation is prepared by mixing bacillus subtilis, bacillus megatherium, bacillus amyloliquefaciens, bacillus licheniformis, enterococcus faecium, lactobacillus brevis, lactobacillus plantarum, lactococcus lactis and saccharomyces cerevisiae through liquid-submerged fermentation. In the liquid preparation, microorganisms are in a dormant state and are stable and easy to preserve, after the preparation is put into water, the dormant strains can quickly resuscitate, harmful substances such as nitrite nitrogen and ammonia nitrogen in the water are quickly decomposed, good algae growth is facilitated, harmful algae reproduction is inhibited, and therefore the purpose of improving the water quality of the culture water is achieved.

Disclosed are methods of making a genetically modified immune cell for modifying a tumor microenvironment (TME) and methods of modifying a tumor microenvironment (TME). In some embodiments, the method can include delivering a first vector to an immune cell, wherein the first vector comprises a nucleic acid encoding a protein that induces T-cell proliferation, promotes persistence and activation of endogenous or adoptively transferred NK or T cells and / or induces production of an interleukin, an interferon, a PD-1 checkpoint binding protein, HMGB1, MyD88, a cytokine or a chemokine. Methods of modulating the suppression of the immune response in a tumor microenvironment, minimizing the proliferation of tumor and suppressive cells, and increasing the efficiency of an anti-cancer therapy, anti-infection therapy, antibacterial therapy, anti-viral therapy, or anti-tumoral therapy are also provided.

Myeloid derived suppressor cell (MDSC) inhibitory agents and vaccine and / or adjuvant enhancers are provided. Improved vaccine treatment regimens employing these agents are also provided. Cancer vaccines and methods for inhibiting tumor growth and cancer metastases are also presented. The myeloid derived suppressor cell (MDSC) inhibiting agents are described as bisphosphonates (such as liposomal clodronate) and CCR2 inhibitors and / or CCR2 antagonists. Methods for enhancing antibody titer levels in response to an antigen of interest are also provided.

A skin care composition and product having beneficial effects on skin conditions / symptoms, such as reducing wrinkles and skin roughness and improving skin elasticity, etc. The composition has three separate parts: a powder part, an aqueous part and an emulsion part. The power part comprises Oligopeptide-1 and Ginseng saponin. The aqueous part comprises Glycyrrhiza glabra Root Extract, Artemisia capillaris Flower Extract, Radix Mori Albae Extract, Zizyphus jujuba Fruit Extract, Scutellaria baicalensis Root Extract, hydrolyzed rice protein and nicotinamide. The emulsion part comprises Bifida ferment lysate, creatine, carnosine, Glucosyl Hesperidin, Hexapeptide-3, Centella asiatica Extract, Coenzyme Q10 capsule, Opuntia Ficus-indica Stem Extract, Rhodiola rosea Extract, Saussurea Involucrata Extract, Panax notoginseng Root Extract and Angelica sinensis Extract capsule.

The invention discloses a natural yeast powder, which is prepared by the method comprising the following steps of: preparing a yeast solution soaking fruit, grains or plant flowers into honey, malt extract and purified water, and extracting the yeast solution of the fruit, grains or plant flowers containing saccharomyces cerevisiae strains and lactobacillus strains after placing; preparing yeast paste: kneading rye flour, the malt extract and the purified water into the yeast paste to be cultivated for four stages; cultivation of microzyme: combining the yeast solution with the yeast paste into wet cenobium for cultivation; enlarging cultivation of the microzyme: mixing the wet cenobium with an enlarging cultivation medium according to a certain ratio to carry out enlarging cultivation of the microzyme multiplying 100-10000 times, and preparing a mixed yeast solution; and concocting yeast powder: mixing dry substances such as glucose, alpha dry rice meal, vitamin C, corn starch, and cane sugar into the mixed yeast solution to finally obtain the natural yeast powder. The natural yeast powder is completely prepared by natural products, does not contain a preservative, and has the advantages of ageing resistance, anti-mildew and long retention period.

The invention relates to a method for preparing anti-influenza virus transfer factor, comprising following steps: preparing raw material, pre-treating, disintegrating, freezing and thawing, extracting, ultra-filtering, disinfecting and packing. The invention employs process of first immune and second extraction. The preparation time is reduced because of low-temperature freezing and thawing and hollow fiber tube ultra-filtering, so the substance activity will not be destroyed. It can be made into oral liquid.

The invention relates to the technical field of biological engineering and the fields of food and medicaments, particularly provides a method for extracting refined yeast grease from yeast, particularly waste saccharomyces cerevisiae and oil production yeast, and further provides soft capsules prepared by compounding the yeast grease, vegetable grease rich in necessary fatty acid for a human bodyand anti-oxidation grease and a preparation method thereof. The obtained yeast grease composite soft capsules have the effects of reducing serum cholesterol and reducing blood fat; the method for producing the yeast grease by using the waste yeast has powerful results of improving the additional value of the waste yeast and generating higher economic benefit; and meanwhile, by comprehensive utilization of the waste yeast, the problem that wastes pollutes environment is solved, and the requirement of circular economy development is met.

The invention belongs to the field of biological detection reagents and relates to a kit for indirect ELISA (Enzyme-Linked Immunosorbent Assay) detection of IgG or IgA antibodies of PRRSV (Porcine reproductive and respiratory syndrome virus) of pigs. The kit is used for indirect ELISA detection of the IgG or IgA antibodies of the PRRSV in serum or saliva of pigs and comprises (a) an antibody detection board, (b) an HRP-labeled goat anti-pig IgG antibody solution or HRP-labeled goat anti-pig IgA, (c) a positive control, namely PRRSV standard positive serum or saliva, (d) a negative control, namely PRRSV negative serum or saliva, (e) sample diluting liquid, (f) 10* concentrated washing liquid, (g) substrate developing liquid, and (h) stopping liquid. The kit is good in PRRSV specificity and sensitivity when being used for detection of PRRSV IgG or IgA antibodies in serum or saliva of pigs. A repeatability test also shows that the kit is good in repeatability.

The invention discloses a facial mask capable of relieving and repairing weak skin and enhancing skin activity and a preparation method of the facial mask. The facial mask consists of the following components: 85-92% of water, 1-1.3% of propylene glycol, 0.5-1.6% of glycerol, 0.3-0.8% of trehalose, 0.2-0.7% of corn glutelin amino acids, 0.2-0.8% of hydroxyethyl cellulose, 0.1-0.6% of allantoin, 0.3-0.8% of panthenol, 0.4-0.9% of aloe vera gel, 0.2-0.8% of elastin, 0.2-0.8% of 3-O-ascorbic acid, 1-1.8% of sodium hyaluronate, 0.6-1.3% of a himanthalia elongata extract, 0.2-0.5% of phenoxyethanol, 0.1-0.5% of a cordate houttuynia extract, 0.2-0.8% of yeast beta-glucan, 0.2-0.7% of a betula bark extract and 0.1-0.3% of essence. The facial mask disclosed by the invention organically combines the aloe vera gel, elastin, himanthalia elongata extract, yeast beta-glucan and betula bark extract for the first time for relieving, repairing and caring weak skin and for enhancing skin activity, so as to solve skin problems of being sensitive, dry, dark yellow, saggy and the like, and the facial mask has achieved innovative technical effects. The preparation method of the facial mask capable of relieving and repairing weak skin and enhancing skin activity can be used for effectively preparing the facial mask disclosed by the invention, and the preparation method is simple in operation.

The invention discloses a method for producing a porcine parvovirus antigen and its product. The method comprises the following steps: porcine parvovirus capsid protein VP2 gene or optimized VP2 gene is cloned in a baculovirus carrier so as to obtain a transfer expression carrier; the constructed transfer expression carrier and baculovirus DNA are carried out cotransfection to obtain recombined baculovirus; the recombined baculovirus is used to infect insect host and cell; the infected insect host is cultured to express corresponding porcine parvovirus capsid protein; and the expressed antigen is ingathered and purified so as to obtain the porcine parvovirus antigen. The method adopts a baculovirus expression system to make safe and efficient porcine parvovirus antigen capsid particles in a domestic silkworm bioreactor; the prepared purified antigen by the method has high safety, and can be directly produced to vaccines for animal immunity. The method for producing porcine parvovirus antigen has the advantages of high expression efficiency, high immunization activity of the expressed antigen, low production cost, large scale production realization and the like.

The invention relates to oil, especially to an emulsified oil which is suitable for being added into livestock feed and a preparation method thereof. The emulsified oil comprises the following components of: by weight, 40-100 parts of vegetable oil, 0.5-5 parts of span, 1-10 parts of monoglyceride stearate, 1-5 parts of sodium caseinate, 1-10 parts of disodium hydrogen phosphate and 30-90 parts of glucose syrup. The emulsified oil is prepared by technological processes of burdening, homogenization, spray drying and cooling. The emulsified oil provided by the invention has balanced nutrient proportion, is rich in medium / short chain aliphatic acid, high nutritional milk protein, digestible monosaccharide and the like which are all easy for animals to absorb, has high net energy value and high digestion and absorption rate, can greatly increase the content of fat in feed and raise energy density of animal diets, is a powered solid, and is convenient to preserve and transport.

The invention provides an adjuvant used for vaccine. The adjuvant comprises alumina gel, levamisole or its derivative, and also polyacrylate. The invention also discloses a vaccine composition containing the adjuvant and an application thereof. The adjuvant can be used as the adjuvant for inactivated vaccine, and also can be used for preparing protein stabilization liquid. The vaccine composition containing the adjuvant can generate humoral immunity for human body and can generate cellular immunity, and can generate good immune response under condition of low antigen content and single immune, and can stabilize protein, degradation and irreversible change cannot be generated.

The invention discloses a genotype VII Newcastle disease virus marker vaccine strain and an application thereof, and belongs to the field of genotype VII Newcastle disease virus marker vaccine strain rescue and application. A built Newcastle disease virus reverse genetic operating platform is utilized for enabling NP protein of a G7 strain to miss 18 amino acids and conducting mutation on F-protein cleavage loci, and the highly-weak virulence and high-virus titer genotype VII Newcastle disease virus marker vaccine strain MG7-NPdelta18+Fmut is rescued through screening. The microbial preservation serial number is CCTCC NO: V201505. The marker vaccine strain has the biological characteristics of high growth titer and low virulence in chick embryos and is genetically stable. The immune protection test result shows that the marker vaccine strain is good in immunogenicity, capable of inducing high-level protective antibodies, and capable of completely protecting immunized chicken, can be used for preventing and controlling a currently-popular genotype VII Newcastle disease virus and lays the foundation of identifying vaccine immunity and wild virus infection.

The invention discloses a prawn feed additive, a preparation method thereof, and a composite feed containing the additive. The additive comprises the following raw materials: astragalus, rheum officinale, Codonopsis pilosula, isatis root, humifuse euphorbia herb and gallnut. The additive may also comprise a synergist. The preparation method comprises: weighing various raw materials according to weight percentage; crushing the raw materials; and feeding the various raw materials into a mixer in turn, and uniformly mixing. The composite feed containing the feed, wherein the weight percentage of the additive is 1 to 3 percent. According to the technical scheme provided by the embodiment of the invention: the additive is made according to a formula of Chinese herb medicine, avoids arousing medicine resistance and producing toxic and side effect, prevents medicine residue in prawns, can obviously increase the growth speed of the prawns and improve the immune activity and disease resistance of the prawns, has high resistance for vibrio anguillarum which is pathogenic bacterium, protects the prawns against diseases, greatly improves survival rate and obviously improves production benefit.

Methods of enhancing antitumor activity of an immune cell comprising contacting the immune cell with a Stat3 inhibitor are described. Also described are methods of killing a tumor cell or inhibiting tumor growth in a subject comprising contacting an immune cell of the subject with a Stat3 inhibitor.

Preparation of agar active polysaccharide is carried out by: pre-treating, extracting for polysaccharide at optimum temperature, removing glue, dialyzing, and drying to obtain final product. The agar active polysaccharide recovery rate reaches to 13%, and polysaccharide content surpasses 76%. It has better breeding effect for mouse lymphocyte and immune activity.

The invention provides a combination containing mushroom polysaccharide and a preparation method thereof. The combination contains mushroom polysaccharide and a carrier, wherein the mushroom is selected from Schizophyllumcommune, Agarics blaze, Cordyceps sinensis, Ganoderma lucidum, Coriolus versicolor, Anthodiacamphorate, Phellinus linteus, Pleurotuscitrinopileatus, Lentinula edodes, Agrocybe aegerita, Hericium erinaceus, Pleurotus eryngiig, Sparrasis crispa, Auricularia auricular, Flammulinavelutipes or a combination thereof.

The present invention relates to compositions containing a novel protein and methods of using those compositions for the diagnosis and treatment of immune related disease

The invention aims to provide a feed additive for removing the earthy taste of freshwater fish. The feed additive comprises, by weight, 5%-10% of allicin, 2%-6% of perilla leaf extracts, 2%-6% of tea polyphenols, 2%-6% of ethyl maltol, 20%-40% of yeast cell walls and 20%-40% of yeast contents. Physical, chemical and biological methods are comprehensively adopted for removing the earthy taste of the freshwater fish effectively through the feed additive, no drug tolerance or toxic and side effect is generated, and the growth rate of fishes can be remarkably increased; antioxidant capacity and immunocompetence of the fishes are improved, disease resistance of fish bodies is enhanced, fish meat quality is improved, the market price of the fishes is increased, and production effectiveness is remarkably promoted.

The invention provides ganoderma lucidum polysaccharide and a preparation method thereof. The ganoderma lucidum polysaccharide is obtained through carrying out water extraction and ultrafiltration on a ganoderma lucidum fruiting body, decoloring and then freeze drying. The decolored ganoderma lucidum polysaccharide has remarkably improved immunocompetence and can stimulate macrophage to generate nitric oxide and stimulate lymphopoiesis.

Selective antagonists of A2A adenosine receptors like those of formula I are provided, wherein Y forms a ring.The novel A2A blockers are useful for the treatment of Parkinsons disease and other diseases.

Disclosed are oligonucleotides for manipulating an immune reaction. The oligonucleotides of the present invention may be useful to stimulate the immune function, to treat the immune-related diseases and the atopic dermatitis, or to protect the normal immune cells.

The invention provides a method for extracting camellia chrysantha polysaccharide from camellia chrysantha leaves. The method comprises the following steps: 1, adding water to the camellia chrysantha leaves for conducting extraction, and obtaining an aqueous extract solution; 2, after concentrating the aqueous extract solution, adding ethanol for conducting precipitation, and obtaining precipitate for standby application; 3, washing the precipitate obtained in step 2 with absolute ethyl alcohol and acetone in sequence, then adding distilled water for conducting dissolution, then adding protease for conducting enzymolysis, and obtaining enzymatic hydrolysate; 4, placing the enzymatic hydrolysate and conducting treatment for 10-60 seconds under ultrasonic waves with the frequency ranging from 1000 kHz to 5000 kHz, then conducting dialysis through a dialysis bag with the molecular cut off being 10000, obtaining reserved liquid for conducting dialysis through a dialysis bag with the molecular cut off being 500000, obtaining penetrating liquid for conducting concentration, adding ethanol for conducting precipitation again, and obtaining precipitate for standby application; 5, washing the precipitate obtained in step 4 with absolute ethyl alcohol and acetone in sequence, then adding distilled water for conducting dissolution, conducting activated carbon decoloration, filtration and drying, and obtaining the camellia chrysantha polysaccharide. The camellia chrysantha polysaccharide obtained through the method has high immunological competence.

A highly efficient and safe HSV derived composite oncolytic vector is provided for the treatment of solid tumors in an individual. The vector comprises two main components being a defective viral genome with multiple reiterations of amplicon type repeat units each carrying inducible toxic genes with cell destructive capabilities and, as a second component, an HSV mutant helper virus which is incapable of replication in non-dividing cells. The vector may be used for the treatment of various kinds of solid tumors including brain malignancies as well as lung, pancreatic, kidney, colon, stomach and other types of cancers. The provided vector is also suitable for administration to an individual in combination with an additional treatment.