Composition containing anti-helicobacter pylori egg yolk globulin and curcumin and application of composition in anti-helicobacter pylori functional food

Abstract

The invention belongs to the technical field of functional food, and particularly relates to a composition containing anti-helicobacter pylori egg yolk globulin and curcumin and application of the composition in anti-helicobacter pylori functional food. The composition is prepared from the following raw materials in parts by weight: 0.1 to 0.3 part of curcumin, 0.01 to 0.03 part of allicin, 0.01 to 0.05 part of ginsenoside Ro, 1 to 3 parts of a biomass extract, 1 to 5 parts of egg yolk globulin powder, 1 to 3 parts of a modified oligosaccharide mixture and 0.1 to 0.5 part of probiotics. The composition can inhibit helicobacter pylori infection and relieve stomach discomfort.

Description

Technical Field

[0001] The invention belongs to the technical field of functional foods, and in particular relates to a composition containing anti-Helicobacter pylori yolk globulin and curcumin and application of the composition in anti-Helicobacter pylori functional foods. Background Art

[0002] Helicobacter pylori ( Helicobacter pylori, Hp ) is a Gram-negative bacterium with multiple flagella, blunt ends, and spirally curved bodies. It is a typical spiral or arc-shaped bacterium on the surface of gastric mucosal epithelial cells, and the curvature of the bacteria is more obvious when observed through tissue sections or biopsy tissue smears. Hp is closely related to the occurrence and development of various gastric diseases such as indigestion, chronic gastritis, peptic ulcers, and gastric cancer.

[0003] Helicobacter pylori can use the rapid movement of its flagella and the urease it produces to cross the gastric mucus layer and colonize in the gastric mucosa. Urease can break down urea to produce ammonia, which helps neutralize gastric acid and provides conditions for Helicobacter pylori to survive and reproduce smoothly in the acidic environment of the stomach; in addition, urease can also destroy the tight junctions between gastric epithelial cells to a certain extent, thereby destroying the gastric epithelial tissue. Once Hp successfully colonizes in the stomach, it will release enzymes and toxins, activate the immune system of the gastric mucosa, trigger chronic inflammatory reactions and tissue damage, leading to chronic gastritis, gastric mucosal erosion, ulcers, atrophy and intestinal metaplasia, further increasing the risk of gastric cancer. In addition, Hp infection can induce extra-gastrointestinal diseases such as unexplained iron deficiency anemia, idiopathic thrombocytopenic purpura and vitamin B12 malabsorption through systemic inflammatory reactions and autoimmune mechanisms.

[0004] The treatment methods for Helicobacter pylori infection have evolved from the initial antibiotic triple therapy and quadruple therapy to the current pentatherapy. Although these methods can clinically cure Helicobacter pylori infection, long-term antibiotic treatment is prone to cause drug resistance and adverse reactions in the human body. In addition, even after antibiotic cure, there is still a high risk of recurrence. These are the problems currently facing medicine. Summary of the invention

[0005] The primary purpose of the present invention is to provide a composition containing anti-Helicobacter pylori yolk globulin and curcumin and the application of the composition in anti-Helicobacter pylori functional food, so as to inhibit Helicobacter pylori infection and relieve stomach discomfort.

[0006] To this end, the present invention provides the following technical solutions.

[0007] One aspect of the present invention provides a composition containing anti-Helicobacter pylori egg yolk globulin and curcumin, comprising the following raw materials in parts by weight: 0.1-0.3 parts of curcumin, 0.01-0.03 parts of allicin, 0.01-0.05 parts of ginsenoside Ro, 1-3 parts of biomass extract, 1-5 parts of egg yolk globulin powder, 1-3 parts of modified oligosaccharide mixture and 0.1-0.5 parts of probiotics.

[0008] In a preferred embodiment of the present invention, the biomass extract is selected from one or a combination of two or more of velvet antler extract, Agrocybe oleracea extract, wolfberry extract, and tangerine peel extract.

[0009] In a preferred embodiment of the present invention, the biomass extract is composed of pilose antler extract, agrocybe angustifolia extract, wolfberry extract and tangerine peel extract in a mass ratio of (2-5): (1-5): (1-3): (1-2).

[0010] In a preferred embodiment of the present invention, the probiotics are selected from one or a combination of two or more of Hericium erinaceus probiotics, Lactobacillus delbrueckii subsp. bulgaricus, Bifidobacterium adolescentis, Lactococcus lactis subsp. cremoris, Streptococcus thermophilus, Clostridium butyricum, Lactobacillus acidophilus, Bifidobacterium longum infantis, and Lactobacillus casei.

[0011] In a preferred embodiment of the present invention, the modified oligosaccharide mixture is an oligosaccharide mixture obtained by enzymatic hydrolysis of starch, and then the modified oligosaccharide mixture is obtained by modifying the oligosaccharide mixture as a substrate.

[0012] In a preferred embodiment of the present invention, the starch is selected from one or a combination of two or more of corn starch, wheat starch, potato starch, barley starch, tapioca starch, oat starch, rice starch, and sweet potato starch.

[0013] In a preferred embodiment of the present invention, the enzyme is α-amylase and / or β-amylase.

[0014] In a preferred embodiment of the present invention, the modification method is to first perform high-speed shearing and then perform phospholipid encapsulation.

[0015] In a preferred embodiment of the present invention, the high-speed shearing conditions are: rotation speed 20000-40000 rpm, time 1-5 min.

[0016] In a preferred embodiment of the present invention, the phospholipid is selected from any one of the phospholipid molecules selected from phosphatidylinositol diphosphate, distearoylphosphatidylglycerol, soybean lecithin, egg yolk lecithin, distearoylphosphatidylcholine, and dipalmitoylphosphatidylcholine.

[0017] In a preferred embodiment of the present invention, the composition is in the form of any one of powder, granules, liquid, semisolid and emulsion.

[0018] Another aspect of the present invention also provides a use of the above-mentioned composition in the preparation of anti-Helicobacter pylori functional food.

[0019] By means of the above technical solution, the present invention has at least the following advantages: 1. The composition of the present invention, which is composed of egg yolk globulin powder as the main active ingredient and supplemented with curcumin, allicin and ginsenoside Ro, has the ability to inhibit Helicobacter pylori and can significantly reduce Helicobacter pylori infection. The egg yolk globulin powder used in the present invention refers to a multivalent anti-Helicobacter pylori antibody extracted physically from the yolk of an egg containing anti-Helicobacter pylori antibodies. Before extraction, the Helicobacter pylori specific antigen needs to be injected into the hen in the early stage to induce the hen to secrete a large amount of specific and functional antibodies. The egg yolk globulin extracted according to the method can not only specifically block pathogen infection, but also increase immunity and play a role in preventing and treating Helicobacter pylori. Ginsenoside Ro is one of the ginseng extracts. Studies have shown that it has anti-inflammatory, detoxifying, and anti-thrombotic effects, inhibits acid-based platelet aggregation, and anti-hepatitis and macrophage activation effects. Curcumin has obvious pharmacological effects in protecting the liver, resisting gastric and duodenal ulcers, resisting fibrosis, promoting immune activity, inhibiting skin mutations induced by ultraviolet rays, lowering blood sugar, and killing nematodes. Allicin is an organic sulfur compound extracted from the bulb (garlic head) of the Allium plant of the Allium family. Allicin has a strong antibacterial effect. It can inhibit a variety of Gram-positive bacteria and Gram-negative bacteria at extremely low concentrations, and also has inhibitory effects on molds, viruses, protozoa, pinworms, etc. Among them, the inhibitory effect on Escherichia coli and Shigella dysenteriae is the most obvious.

[0020] 2. Another aspect of the present invention is that biomass extracts such as antler extracts and Agrocybe aegerita extracts are added to the composition. The main active ingredients of antler are antler polypeptides, antler polysaccharides, antler polyamines, proteins, amino acids, sex hormones and fat-soluble components. Studies have shown that antler extracts play an important role in immunomodulation, anti-cancer, anti-inflammatory, etc. Agrocybe aegerita extracts have strong antioxidant capacity and contain polysaccharides with anti-cancer activity. It tastes delicious, has the effects of nourishing yang and strengthening yin, beauty and health care, and has ideal therapeutic functions for anti-cancer, blood pressure reduction, etc. Therefore, adding biomass extracts can enhance human immunity. At the same time, a modified oligosaccharide mixture and probiotics are added. The addition of probiotics can regulate intestinal flora, ensure the temperature of intestinal flora, and increase the number of active probiotics. In addition to providing an energy source for probiotics and ensuring the number and activity of probiotics, the modified oligosaccharide mixture can also ensure that it has the effects of regulating blood sugar and controlling weight in the case of long-term consumption.

[0021] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention and implement it according to the contents of the specification, the preferred embodiments of the present invention are described in detail below. DETAILED DESCRIPTION

[0022] In order to make the technical means, creative features, objectives and effects of the present invention easy to understand, the technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Unless otherwise specified, the percentage content involved in the present invention refers to mass percentage for solid-liquid mixing and solid-solid mixing, and refers to volume percentage for liquid-liquid mixing.

[0024] Unless otherwise specified, the percentage concentrations referred to in the present invention all refer to final concentrations, which refer to the percentage of the added component in the system after the addition of the component.

[0025] The temperature parameters in the present invention, if not specifically limited, allow for both constant temperature treatment and treatment within a certain temperature range. The constant temperature treatment allows the temperature to fluctuate within the precision range controlled by the instrument.

[0026] Unless otherwise specified, the experimental methods used in the following examples are all conventional methods.

[0027] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0028] Unless otherwise specified, the pilose antler extract used in the following examples was prepared according to the following method: Grind the tea tree mushroom into powder, and add distilled water in a ratio of 1:20 to water and mix evenly. Extract at 80°C for 4 hours, and then centrifuge at 5000rpm for 10 minutes to obtain a first supernatant and a first filter residue. Add distilled water to the first filter residue in a ratio of 1:20 to water, extract at 80°C for 4 hours, and then centrifuge at 5000rpm for 10 minutes to obtain a second supernatant, and combine the two supernatants to obtain a supernatant mixture. Shear the obtained supernatant mixture in a high-speed shearing machine at a speed of 20,000rpm for 5 minutes, then centrifuge at 9500rpm for 10 minutes, collect the supernatant, and vacuum concentrate the obtained supernatant to 30% of the original volume to obtain a concentrated solution. Anhydrous ethanol was added to the obtained concentrated solution to make the ethanol concentration in the system reach 80%, and the mixture was precipitated for 12 hours and centrifuged for about 20 minutes to obtain a precipitate. The precipitate was redissolved and concentrated in a vacuum to remove the ethanol. Finally, the obtained product was pre-frozen at -80°C for 1 hour, and then freeze-dried in a freeze dryer at -50°C in a vacuum environment for 3 days to obtain the Agrocybe agrocybe extract.

[0029] According to the same method as above, pilose antler extract, wolfberry extract and tangerine peel extract were prepared respectively.

[0030] Unless otherwise specified, the modified oligosaccharide mixture used in the following examples is prepared according to the following method: corn starch, wheat starch and potato starch are mixed in equal mass ratios and added to water to prepare a 30% starch solution, the pH value of the solution is adjusted to 6.0, 0.2% α-amylase is added, and the reaction is carried out at 60°C for 120 minutes. After the reaction is completed, the enzyme is inactivated to obtain an enzyme reverse mixture solution. 80 mesh quartz sand is added to the obtained enzyme reverse mixture solution in an amount of 1ml:0.01g by volume and sheared at 30000rpm in a high-speed shearing machine for 3min. After shearing, the obtained supernatant is centrifuged and passed through a 0.5μm microfiltration membrane to obtain a filtrate. Soy lecithin is added to the obtained filtrate in an amount of 100mL:3g by volume and stirred at room temperature for 18h to obtain a mixed solution. Finally, the obtained mixed solution is freeze-dried to obtain a modified oligosaccharide mixture.

[0031] Unless otherwise specified, the probiotics used in the following examples have a viable count of 5×10 9 The probiotic powder is obtained by mixing Hericium erinaceus probiotics with CFU / g, Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842, Bifidobacterium adolescentis ATCC 15703, Lactococcus lactis subsp. cremoris CGMCC 1.3991, Streptococcus thermophilus ATCC 14485, Clostridium butyricum, Lactobacillus acidophilus, Bifidobacterium longum infantis, and Lactobacillus casei. All of the above bacteria were purchased from Beijing Biobowei Biotechnology Co., Ltd.

[0032] Embodiment 1:

[0033] Preparation of biomass extract: Combining pilose antler extract, Agrocybe oleracea extract, wolfberry extract and tangerine peel extract in a mass ratio of 3.5:3:2:1.5 to obtain a biomass extract.

[0034] Preparation of modified oligosaccharide mixture: corn starch, wheat starch and potato starch were mixed in equal mass ratios and added to water to prepare a 30% starch solution. The pH value of the solution was adjusted to 6.0, and 0.2% of the volume of the obtained starch solution was added with α-amylase, and the reaction was carried out at 60°C for 120 minutes. After the reaction was completed, the enzyme was inactivated to obtain an enzyme reverse mixture solution. 80 mesh quartz sand was added to the obtained enzyme reverse mixture solution in an amount of 1ml:0.01g by volume and sheared at 30000rpm in a high-speed shearing machine for 3min. After shearing, the obtained supernatant was centrifuged and passed through a 0.5μm microfiltration membrane to obtain a filtrate. Soy lecithin was added to the obtained filtrate in an amount of 100mL:3g by volume and stirred at room temperature for 18h to obtain a mixed solution. Finally, the obtained mixed solution was freeze-dried to obtain a modified oligosaccharide mixture.

[0035] According to weight portions, 0.2 portions of curcumin, 0.02 portions of allicin, 0.03 portions of ginsenoside Ro, 2 portions of biomass extract, 3 portions of egg yolk globulin powder, 2 portions of modified oligosaccharide mixture and 0.3 portions of probiotics are mixed, and dry granulated to obtain a composite powder with an average particle size of 80 meshes.

[0036] Embodiment 2:

[0037] Preparation of biomass extract: Combining pilose antler extract, Agrocybe oleracea extract, wolfberry extract and tangerine peel extract in a mass ratio of 5:1:3:2 to obtain a biomass extract.

[0038] Preparation of modified oligosaccharide mixture: barley starch, oat starch, cassava starch and sweet potato starch were mixed in equal mass ratios and added to water to prepare a 40% starch solution. The pH value of the solution was adjusted to 6.0, and 0.3% of the volume of the obtained starch solution was added with β-amylase, and the reaction was carried out at 60°C for 120 minutes. After the reaction, the enzyme was inactivated to obtain an enzyme reverse mixture solution. 80 mesh quartz sand was added to the obtained enzyme reverse mixture solution in an amount of 1ml:0.02g by volume and sheared at 40000rpm in a high-speed shearing machine for 1min. After shearing, the obtained supernatant was centrifuged and passed through a 0.5μm microfiltration membrane to obtain a filtrate. Phosphatidylinositol diphosphate was added to the obtained filtrate in an amount of 100mL:2g by volume and stirred at room temperature for 18h to obtain a mixed solution. Finally, the obtained mixed solution was dried by freeze drying to obtain a modified oligosaccharide mixture.

[0039] According to weight portions, 0.1 parts of curcumin, 0.03 parts of allicin, 0.01 parts of ginsenoside Ro, 3 parts of biomass extract, 1 part of egg yolk globulin powder, 3 parts of modified oligosaccharide mixture and 0.1 parts of probiotics are mixed, and dry granulated to obtain a composite powder with an average particle size of 80 meshes.

[0040] Embodiment 3:

[0041] Preparation of biomass extract: Combining pilose antler extract, Agrocybe oleracea extract, wolfberry extract and tangerine peel extract in a mass ratio of 2:5:3:1 to obtain a biomass extract.

[0042] Preparation of modified oligosaccharide mixture: Cassava starch, rice starch, oat starch and barley starch were mixed in equal mass ratios and added to water to prepare a 20% starch solution. The pH value of the solution was adjusted to 6.0, and 0.2% of the volume of the obtained starch solution was added. α-amylase and 0.1% of β-amylase were reacted at 60°C for 120 minutes. After the reaction, the enzyme was inactivated to obtain an enzyme reverse mixture solution. 80 mesh quartz sand was added to the obtained enzyme reverse mixture solution in an amount of 1ml:0.03g by volume and sheared at 20000rpm in a high-speed shearing machine for 5min. After shearing, the obtained supernatant was centrifuged and passed through a 0.5μm microfiltration membrane to obtain a filtrate. Distearoylphosphatidylglycerol was added to the obtained filtrate in an amount of 100mL:4g by volume and stirred at room temperature for 18h to obtain a mixed solution. Finally, the obtained mixed solution was dried by freeze drying to obtain a modified oligosaccharide mixture.

[0043] According to weight portions, 0.3 parts of curcumin, 0.01 parts of allicin, 0.05 parts of ginsenoside Ro, 1 part of biomass extract, 5 parts of egg yolk globulin powder, 1 part of modified oligosaccharide mixture and 0.5 parts of probiotics are mixed, and dry granulated to obtain a composite powder with an average particle size of 80 meshes.

[0044] Comparative Example 1: Preparation of modified oligosaccharide mixture: corn starch, wheat starch and potato starch were mixed in equal mass ratios and added to water to prepare a 30% starch solution. The pH value of the solution was adjusted to 6.0, 0.2% α-amylase was added, and the reaction was carried out at 60°C for 120 minutes. After the reaction, the enzyme was inactivated to obtain an enzyme reverse mixture solution. 80 mesh quartz sand was added to the obtained enzyme reverse mixture solution in an amount of 1ml:0.01g by volume and sheared at 30000rpm in a high-speed shearing machine for 3min. After shearing, the obtained supernatant was centrifuged and passed through a 0.5μm microfiltration membrane to obtain a filtrate. Soy lecithin was added to the obtained filtrate in an amount of 100mL:3g by volume and stirred at room temperature for 18h to obtain a mixed solution. Finally, the obtained mixed solution was dried by freeze drying to obtain a modified oligosaccharide mixture.

[0045] According to weight portions, 0.2 portions of curcumin, 0.02 portions of allicin, 0.03 portions of ginsenoside Ro, 3 portions of egg yolk globulin powder, 2 portions of modified oligosaccharide mixture and 0.3 portions of probiotics are mixed, and dry granulated to obtain a composite powder with an average particle size of 80 meshes.

[0046] Comparative Example 2: Preparation of biomass extract: Combining pilose antler extract, Agrocybe oleracea extract, wolfberry extract and tangerine peel extract in a mass ratio of 3.5:3:2:1.5 to obtain a biomass extract.

[0047] According to weight portions, 0.2 portions of curcumin, 0.02 portions of allicin, 0.03 portions of ginsenoside Ro, 2 portions of biomass extract, 3 portions of egg yolk globulin powder and 0.3 portions of probiotics are mixed, and dry granulated to obtain a composite powder with an average particle size of 80 meshes.

[0048] Comparative Example 3: Preparation of biomass extract: Combining pilose antler extract, Agrocybe oleracea extract, wolfberry extract and tangerine peel extract in a mass ratio of 3.5:3:2:1.5 to obtain a biomass extract.

[0049] Preparation of modified oligosaccharide mixture: corn starch, wheat starch and potato starch were mixed in equal mass ratios and added to water to prepare a 30% starch solution. The pH value of the solution was adjusted to 6.0, 0.2% α-amylase was added, and the reaction was carried out at 60°C for 120 minutes. After the reaction, the enzyme was inactivated to obtain an enzyme reverse mixture solution. 80 mesh quartz sand was added to the obtained enzyme reverse mixture solution in an amount of 1ml:0.01g by volume and sheared at 30000rpm in a high-speed shearing machine for 3min. After shearing, the obtained supernatant was centrifuged and passed through a 0.5μm microfiltration membrane to obtain a filtrate. Soy lecithin was added to the obtained filtrate in an amount of 100mL:3g by volume and stirred at room temperature for 18h to obtain a mixed solution. Finally, the obtained mixed solution was dried by freeze drying to obtain a modified oligosaccharide mixture.

[0050] According to weight portions, 0.2 portions of curcumin, 0.02 portions of allicin, 2 portions of biomass extract, 3 portions of egg yolk globulin powder, 2 portions of modified oligosaccharide mixture and 0.3 portions of probiotics are mixed, and dry granulated to obtain a composite powder with an average particle size of 80 meshes.

[0051] Comparative Example 4: Preparation of biomass extract: Combining pilose antler extract, Agrocybe oleracea extract, wolfberry extract and tangerine peel extract in a mass ratio of 3.5:3:2:1.5 to obtain a biomass extract.

[0052] Preparation of modified oligosaccharide mixture: corn starch, wheat starch and potato starch were mixed in equal mass ratios and added to water to prepare a 30% starch solution. The pH value of the solution was adjusted to 6.0, 0.2% α-amylase was added, and the reaction was carried out at 60°C for 120 minutes. After the reaction, the enzyme was inactivated to obtain an enzyme reverse mixture solution. 80 mesh quartz sand was added to the obtained enzyme reverse mixture solution in an amount of 1ml:0.01g by volume and sheared at 30000rpm in a high-speed shearing machine for 3min. After shearing, the obtained supernatant was centrifuged and passed through a 0.5μm microfiltration membrane to obtain a filtrate. Soy lecithin was added to the obtained filtrate in an amount of 100mL:3g by volume and stirred at room temperature for 18h to obtain a mixed solution. Finally, the obtained mixed solution was dried by freeze drying to obtain a modified oligosaccharide mixture.

[0053] According to weight portions, 0.2 portions of curcumin, 0.03 portions of ginsenoside Ro, 2 portions of biomass extract, 3 portions of egg yolk globulin powder, 2 portions of modified oligosaccharide mixture and 0.3 portions of probiotics are mixed, and dry granulated to obtain a composite powder with an average particle size of 80 meshes.

[0054] Comparative Example 5: Preparation of biomass extract: Combining pilose antler extract, Agrocybe oleracea extract, wolfberry extract and tangerine peel extract in a mass ratio of 3.5:3:2:1.5 to obtain a biomass extract.

[0055] Preparation of modified oligosaccharide mixture: corn starch, wheat starch and potato starch were mixed in equal mass ratios and added to water to prepare a 30% starch solution. The pH value of the solution was adjusted to 6.0, 0.2% α-amylase was added, and the reaction was carried out at 60°C for 120 minutes. After the reaction, the enzyme was inactivated to obtain an enzyme reverse mixture solution. 80 mesh quartz sand was added to the obtained enzyme reverse mixture solution in an amount of 1ml:0.01g by volume and sheared at 30000rpm in a high-speed shearing machine for 3min. After shearing, the obtained supernatant was centrifuged and passed through a 0.5μm microfiltration membrane to obtain a filtrate. Soy lecithin was added to the obtained filtrate in an amount of 100mL:3g by volume and stirred at room temperature for 18h to obtain a mixed solution. Finally, the obtained mixed solution was dried by freeze drying to obtain a modified oligosaccharide mixture.

[0056] According to weight portions, 0.02 portions of allicin, 0.03 portions of ginsenoside Ro, 2 portions of biomass extract, 3 portions of egg yolk globulin powder, 2 portions of modified oligosaccharide mixture and 0.3 portions of probiotics are mixed, and dry granulated to obtain a composite powder with an average particle size of 80 meshes.

[0057] Test 1: Antibacterial detection of the composition against Helicobacter pylori (1) Preparation of bacterial solution: Helicobacter pylori was cultured to the logarithmic phase, 5 mL of bacterial solution was taken, centrifuged at 5000 g for 5 min, and the bacteria were resuspended in TSB medium and the concentration of the bacterial solution was adjusted to 1×10 7 CFU / mL.

[0058] Preparation of composition solution: The composition obtained in Example 1 was dissolved in water to obtain a composition solution with a concentration of 20 mg / L. Examples 2-3 and Comparative Examples 1-5 were prepared in the same manner to obtain corresponding composition solutions.

[0059] (2) Take a 96-well plate, add 100 μL of the bacterial solution in step (1) above, 95 μL of TSB medium, and 5 μL of the composition solution of Examples 1-3 and Comparative Examples 1-5 to each well, and add 5 μL of DMSO solution to each well. For the blank control, set 3 replicates for each treatment. Place the 96-well plate at 37° C. and shake at 150 rpm for 48 h.

[0060] (3) The OD600 value reflects the concentration of the bacterial solution, and the inhibition rate (%) is calculated according to the following formula: Inhibition rate = (OD600 control bacterial solution - OD600 treated bacterial solution) / OD600 control bacterial solution × 100%.

[0061] The test results are shown in Table 1 below.

[0062] Table 1 Inhibition rate of Helicobacter pylori by different compositions Group Antibacterial rate (%) Example 1 83.1 Example 2 79.4 Example 3 82.6 Comparative Example 1 60.4 Comparative Example 2 77.9 Comparative Example 3 66.7 Comparative Example 4 69.7 Comparative Example 5 72.8 Blank Group -- Note: In the table, -- means no obvious antibacterial effect It can be seen from the results in Table 1 that the compositions of Examples 1-3 of the present invention have more obvious Helicobacter pylori inhibition effects than the compositions of Comparative Examples 1-5.

[0063] Test 2: Functional food containing the composition and its antibacterial test on Helicobacter pylori The compositions of Example 1 and Comparative Examples 1-5 were added to the black sesame powder in an amount of 10 mg of the composition per 100 g of the black sesame powder and mixed evenly to prepare black sesame powder containing the compositions.

[0064] 70 patients who were randomly selected after urea in hospital 14 C] breath test, confirmed as Helicobacter pylori positive subjects. Aged between 30-50, male to female ratio of 40:30. The above subjects were randomly divided into 7 groups, of which groups 1-6 took the black sesame powder containing each composition described in this application; the other blank group took only pure black sesame, each group took it once a day in the morning, and the dosage was the same. During the trial, the diet and lifestyle of each group of subjects were kept the same as much as possible. After oral administration for 30 days, urea[ 14 C] breath test. The number of positive subjects in each group was counted, and the results are shown in Table 2: Table 2 Clinical effect test of the composition Group Number of Hp(+) subjects Example 1 1 Comparative Example 1 8 Comparative Example 2 3 Comparative Example 3 5 Comparative Example 4 5 Comparative Example 5 4 Blank Group 10 It can be seen from the results in Table 2 that, compared with the functional foods composed of the compositions of Comparative Examples 1-5 and the functional foods of the blank group, the number of positive subjects after taking the functional food prepared by the composition of Example 1 of the present invention for 30 days was significantly reduced. This result shows that the composition of the present invention and the functional food containing the composition have a good anti-Helicobacter pylori effect.

[0065] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technician familiar with this profession can make some changes or modifications to equivalent embodiments of equivalent changes by using the methods and technical contents disclosed above without departing from the scope of the technical solution of the present invention. However, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A composition containing anti-Helicobacter pylori yolk globulin and curcumin, characterized in that: The invention comprises the following raw materials in parts by weight: 0.1-0.3 parts of curcumin, 0.01-0.03 parts of allicin, 0.01-0.05 parts of ginsenoside Ro, 1-3 parts of biomass extract, 1-5 parts of egg yolk globulin powder, 1-3 parts of modified oligosaccharide mixture and 0.1-0.5 parts of probiotics.

2. The composition according to claim 1, characterized in that The biomass extract is selected from one or a combination of two or more of velvet antler extract, Agrocybe oleracea extract, wolfberry extract and tangerine peel extract.

3. The composition according to claim 1 or 2, characterized in that The biomass extract is composed of pilose antler extract, agrocybe angustifolia extract, wolfberry extract and tangerine peel extract in a mass ratio of (2-5): (1-5): (1-3): (1-2).

4. The composition according to claim 1, characterized in that The probiotics are selected from one or a combination of two or more of Hericium erinaceus probiotics, Lactobacillus delbrueckii subsp. bulgaricus, Bifidobacterium adolescentis, Lactococcus lactis subsp. cremoris, Streptococcus thermophilus, Clostridium butyricum, Lactobacillus acidophilus, Bifidobacterium longum infantis, and Lactobacillus casei.

5. The composition according to claim 1, characterized in that The modified oligosaccharide mixture is an oligosaccharide mixture obtained by enzymatic hydrolysis of starch, and then the modified oligosaccharide mixture is obtained by modifying the oligosaccharide mixture as a substrate.

6. The composition according to claim 4, characterized in that The starch is selected from one or a combination of two or more of corn starch, wheat starch, potato starch, barley starch, tapioca starch, oat starch, rice starch and sweet potato starch.

7. The composition according to claim 4, characterized in that The enzyme is α-amylase and / or β-amylase.

8. The composition according to claim 4, characterized in that The modification method comprises firstly performing high-speed shearing and then performing phospholipid encapsulation.

9. The composition according to claim 6, characterized in that The high-speed shearing conditions are: rotation speed 20000-40000 rpm, time 1-5 min.

10. The composition according to claim 6, characterized in that The phospholipid is selected from any one of phospholipid molecules selected from phosphatidylinositol diphosphate, distearoylphosphatidylglycerol, soybean lecithin, egg yolk lecithin, distearoylphosphatidylcholine, and dipalmitoylphosphatidylcholine.

11. The composition according to claim 1, characterized in that The composition is in the form of powder, granules, liquid, semisolid or emulsion.

12. Use of the composition according to any one of claims 1 to 9 in the preparation of anti-Helicobacter pylori functional food.