Hydrogel composition and preparation method thereof

Abstract

The invention provides a hydrogel composition. The hydrogel composition is prepared from the following components in percentage by weight: 0.5 to 15 percent of an adhesion component, 0.5 to 10 percent of a moisturizing component, 0.01 to 5 percent of a bioactive component, 0.01 to 5 percent of a physical crosslinking agent, 0.01 to 2 percent of a flavoring component, 0.01 to 2 percent of an antimicrobial component and the balance of medical purified water, wherein the bioactive component comprises at least one of lipoic acid, tea polyphenol, lactic acid bacteria extract and metabiotics; the antimicrobial component comprises at least one of copper ions, benzyl alcohol, p-hydroxyacetophenone, sodium methyl hydroxybenzoate and sodium ethyl hydroxybenzoate. According to the hydrogel composition disclosed by the invention, the low-concentration antimicrobial component is introduced, so that the antimicrobial component has more stable and effective bactericidal activity and can be more stably adhered to a wound, and the hydrogel composition can have better antibacterial performance and a function of promoting wound healing without additionally adding antibiotics.

Description

Technical Field

[0001] The present invention relates to the medical field, and in particular to a hydrogel composition and a preparation method thereof. Background Art

[0002] Research data show that the main obstacle to slow wound healing is that bacteria on the wound surface secrete extracellular polymers to wrap them and form a certain structure of cell aggregates, namely biofilm, which greatly increases the ability of bacteria to resist adverse external conditions. At present, the effectiveness of antibiotics against biofilm is insufficient; if it depends on increasing the dose, it is easy to lead to increased side effects, and the impact of environmental resistance is becoming more and more significant. Probiotics and their metabolites have diverse functions, especially the extracellular polysaccharides produced by lactic acid bacteria can inhibit biofilm formation, and their anti-inflammatory properties promote wound healing by inhibiting the proliferation of pathogens and showing antibacterial effects on wounds. Postbiotics can contain many components, such as metabolites, cell components, cell lysates, short-chain fatty acids, extracellular polysaccharides, teichoic acids, proteins and peptidoglycan-derived cell peptides, and pili-type structures. Although postbiotics do not contain live microorganisms, they have beneficial effects on host health through mechanisms similar to those of probiotics, thereby reducing the risks that may exist after contact with live microorganisms. The local application of postbiotics can be regarded as a novel treatment method to accelerate the healing process in wound research.

[0003] In the related art, antibiotics are usually added to antibacterial hydrogels. Although they have a certain antibacterial effect, their antibacterial components are often not centrally processed after actual use. They are metabolized by the human body and then discarded into the environment, resulting in a certain concentration of residues. Various data show that the problem of microbial resistance caused by low-concentration antibiotic residues has become increasingly serious. Providing an environmentally sustainable antibacterial material by using composite polymers has always been the goal pursued by experts in this field. Summary of the invention

[0004] The present invention aims to solve at least one of the above technical problems.

[0005] To this end, the first object of the present invention is to provide a hydrogel composition The second object of the present invention is to provide a method for preparing a hydrogel composition.

[0006] To achieve the first object of the present invention, the present invention provides a hydrogel composition, comprising: an adhesive component: 0.5wt.%-15wt.%, a moisturizing component: 0.5wt.%-10wt.%, a bioactive component: 0.01wt.%-5wt.%, a physical cross-linking agent: 0.01wt.%-5wt.%, a flavoring component: 0.01wt.%-2wt.%, an antimicrobial component: 0.01wt.%-2wt.%, medical purified water, and the rest; wherein the bioactive component comprises at least one of lipoic acid, tea polyphenols, a lactic acid bacteria extract and a postbiotic; the antimicrobial component comprises at least one of copper ions, benzyl alcohol, p-hydroxyacetophenone, sodium methylparaben, and sodium ethylparaben; and a method for preparing the hydrogel composition comprises: S100, adding the adhesive component and the physical cross-linking agent into medical purified water, stirring evenly, then adding part of the antimicrobial component, and continuing to stir evenly to obtain a polymer transparent liquid; S200, adding the moisturizing component, the bioactive component, the flavor correcting component and the remaining antimicrobial component into the polymer transparent liquid, stirring evenly to obtain a hydrogel composition.

[0007] Compared with the prior art, the technical effect achieved by adopting the technical scheme is as follows: the hydrogel composition of the present invention comprises an adhesive component, a moisturizing component, a biological component, a physical crosslinking agent, a flavoring component, an antimicrobial component and medical purified water. By reasonably selecting an appropriate ratio of the adhesive component and the physical crosslinking agent, the adhesion ability of the hydrogel composition can be improved, so that the hydrogel composition can better adhere to the human skin. Under the action of the physical crosslinking agent, the adhesive component is crosslinked to form a network structure, which has better biocompatibility, is non-toxic or has low toxicity, and is more suitable for use in the medical field; a wound surface kept moist can heal faster than a dry wound exposed to the air. Therefore, the additional addition of moisturizing ingredients to the hydrogel composition can keep the hydrogel composition moist for a long time, further improving the healing speed of the wound; the presence of the flavoring ingredient can remove the odor in the hydrogel composition, making it more comfortable and pleasant during use; the bioactive ingredients bring antioxidant, anti-inflammatory and skin repair effects to the hydrogel composition, and the bioactive ingredients include at least one of lipoic acid, tea polyphenols, lactic acid bacteria extract and postbiotics, among which lipoic acid can scavenge free radicals, reduce the negative impact of oxidative stress on wound healing, and promote the synthesis of collagen, enhancing the skin's repair ability; tea polyphenols can inhibit inflammatory reactions, reduce It can reduce redness, swelling and pain around wounds and promote wound healing; lactic acid bacteria extract can regulate the acid-base balance of the skin, promote cell regeneration and metabolism, increase the water content of the skin, keep the skin moist, and help wound healing; postbiotics can increase the number of beneficial bacteria, thereby improving the skin's immune function and repair ability; antimicrobial ingredients can promote wound healing by inhibiting the growth and reproduction of microorganisms and reducing the risk of infection. Antimicrobial ingredients can provide an effective protective barrier to prevent bacteria and other microorganisms from entering the wound, and maintain a clean and moist environment for the wound, which helps the wound healing process proceed smoothly. Antimicrobial ingredients include copper ions At least one of methylparaben, benzyl alcohol, p-hydroxyacetophenone, sodium methylparaben, and sodium ethylparaben, wherein copper ions can destroy microbial cell walls and cell membranes, leading to their death, and can also promote angiogenesis and tissue regeneration in the process of wound healing; benzyl alcohol and p-hydroxyacetophenone can not only destroy microbial cell membranes, leading to their death, but also promote inflammatory response and repair of tissue damage in the process of wound healing; sodium methylparaben can effectively inhibit the growth of various bacteria, it can destroy microbial cell membranes, leading to their death, and can also promote inflammation relief and skin tissue repair in the process of wound healing; sodium ethylparaben can destroy microbial cell membranes, leading to their death.The hydrogel composition of the present invention introduces low-concentration antimicrobial components, and after the adhesive components form a viscous colloid, the antimicrobial components have more stable and effective bactericidal activity, can be more stably adhered to the wound, and the effective components are controlled to slowly seep out and act on the wound surface, without the need to add additional antibiotics, so that the hydrogel composition can have good antibacterial properties and the function of promoting wound healing.

[0008] In addition, the technical solution provided by the present invention may also have the following technical features: In the above technical features, the antimicrobial component includes at least one of nano copper particles and nano benzyl alcohol particles, nano p-hydroxyacetophenone particles, nano sodium methylhydroxybenzoate particles, and nano sodium ethylhydroxybenzoate particles.

[0009] Compared with the prior art, the technical effects achieved by adopting the technical scheme are as follows: when copper ions are directly loaded on the temperature-responsive hydrogel, the free copper ions have a certain anti-polymerization effect, affecting the formation of the hydrogel network during the polymerization process, while the nano-copper particles can achieve a balance between sustained antibacterial properties and biocompatibility. Therefore, the nano-copper particles are introduced into the hydrogel composition, which can achieve excellent antibacterial properties while maintaining low cytotoxicity, and can be applied to the field of medical materials; benzyl alcohol, parahydroxyacetophenone, sodium methylparaben, and sodium ethylparaben also use nanoparticles, which can not only control the antimicrobial components to have a better sustained-release effect, but also further enhance their antimicrobial effects, so that the hydrogel composition can continuously release the antimicrobial components for a long time and have a better effect.

[0010] In any of the above technical features, the nano copper particles account for 75wt.%-90wt.% of the total antimicrobial components.

[0011] Compared with the existing technology, the technical effects achieved by adopting this technical solution are as follows: nano-copper particles have more stable and effective bactericidal activity, and the strong antibacterial properties can reduce the risk of cross-infection between wounds. A high proportion of nano-copper particles can ensure the continuous release of copper ions during the wound healing process and inhibit bacterial growth for a long time. In addition, nano-copper particles help to enhance the stability of the hydrogel, making it more suitable for wound application. Nano-copper particles can also increase the shelf life of the hydrogel, facilitating the long-term storage and transportation of the hydrogel composition.

[0012] In any of the above technical features, the hydrogel composition further includes: a pH regulator: 0.01wt.%-2wt.%; the pH regulator is a buffer solution regulator.

[0013] Compared with the prior art, the technical effect achieved by adopting this technical solution is as follows: in the hydrogel combination, the ideal pH for copper ion release should be weakly acidic or neutral. Therefore, in order to optimize the antibacterial properties, stability and biocompatibility of the hydrogel composition, thereby ensuring its effectiveness and safety in practical applications, the hydrogel composition should also include a pH regulator; the use of the above-mentioned type of pH regulator helps to prevent undesirable chemical reactions caused by pH changes, thereby ensuring the chemical stability of the hydrogel, so that the pH value of the hydrogel composition can be stabilized within a range that is friendly to human cells, thereby reducing cytotoxicity and improving biocompatibility.

[0014] In any of the above technical features, the adhesive component includes at least one of chitosan, oligochitosan, carboxymethyl chitosan, chitosan acetate, polyvinyl alcohol, hydroxypropyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose, carbomer, sodium alginate, polyethylene glycol, and polyvinyl pyrrolidone; and / or the moisturizing component includes at least one of glycerin, propylene glycol, 1,2-hexanediol, and hyaluronic acid; and / or the physical cross-linking agent includes at least one of calcium chloride, calcium carbonate, sodium sulfate, sodium tripolyphosphate, and citric acid; and / or the flavoring component includes at least one of a sweetener, menthol, and a flavoring agent.

[0015] Compared with the prior art, the technical effects achieved by the technical solution are as follows: the above-mentioned adhesive component can enhance the adhesion between the hydrogel composition and the wound surface, making it difficult to fall off during the application process, and can prolong the action time of the hydrogel composition, and continuously inhibit wound infection; these adhesive components usually have good biocompatibility and biological activity, can promote the proliferation and migration of immune cells, and accelerate the wound repair process; the above-mentioned moisturizing component is conducive to gas exchange and absorption of exudates, and the moist environment is conducive to cell proliferation and ECM (Extracellular matrix) synthesis, thereby accelerating wound healing; the above-mentioned cross-linking agent can be used to cross-link the adhesive polymer component to form a network structure, which has better biocompatibility, is non-toxic or low-toxic, and is more suitable for use in the medical field; the above-mentioned flavoring component can improve the taste and smell of the hydrogel composition and improve the patient's comfort. Some flavoring components, such as menthol, also have a cooling and itching effect, which can relieve the itching symptoms of the wound surface.

[0016] To achieve the second object of the present invention, the present invention provides a method for preparing a hydrogel composition, which is used to prepare a hydrogel composition according to any one of the above technical features, and the preparation method comprises: S100, adding the adhesive component and the physical cross-linking agent into medical purified water, stirring evenly, then adding part of the antimicrobial component, and continuing to stir evenly to obtain a polymer transparent liquid; S200, adding the moisturizing component, the bioactive component, the flavor correcting component and the remaining antimicrobial component into the polymer transparent liquid, stirring evenly to obtain a hydrogel composition.

[0017] Compared with the prior art, the technical effects achieved by adopting the technical scheme are as follows: the preparation method of the present invention has simple steps, and the hydrogel composition can be prepared only through a simple stirring step; the antimicrobial component is added in two parts respectively, which can further make the antimicrobial component evenly distributed in the prepared hydrogel composition; the preparation method of the present invention fully considers the performance characteristics of the hydrogel and the needs of wound healing, and ensures the effectiveness and safety of the product by adding components with different functions in steps.

[0018] In any of the above technical features, the mass ratio of the adhesive component, the physical cross-linking agent and the medical purified water is 0.1-3: 0.002-1: 0.2-20; and / or the mass ratio of the moisturizing component, the bioactive component and the flavoring component is 0.1-2: 0.002-1: 0.002-0.4; and / or the mass ratio of the antimicrobial component to the medical purified water is 0.002-0.4: 0.2-20; wherein the antimicrobial component comprises at least one of copper ions, benzyl alcohol, parahydroxyacetophenone, sodium methylparaben and sodium ethylparaben.

[0019] Compared with the prior art, the technical effect achieved by adopting this technical solution is as follows: by designing the dosage ratio between the components of the hydrogel composition, the prepared hydrogel composition can effectively adhere to the wound surface, which can not only maintain the moist environment of the wound and promote cell growth and migration, but also promote cell proliferation and differentiation while providing effective antibacterial protection, thereby ensuring the safety and effectiveness of the hydrogel composition in clinical applications.

[0020] In any of the above technical features, the antimicrobial component includes nano copper particles and at least one of nano benzyl alcohol particles, nano p-hydroxyacetophenone particles, nano sodium methylhydroxybenzoate particles, and nano sodium ethylhydroxybenzoate particles; wherein the preparation method of the nano copper particles includes: S101, adding a copper-containing compound to an ethanol solution of 1,3,5-benzenetricarboxylic acid, stirring the mixture, and obtaining a suspension solution; S102, sequentially centrifuging, washing and drying the suspended solution to obtain nano copper particles.

[0021] Compared with the prior art, the technical effects achieved by adopting the technical solution are as follows: adopting the above-mentioned nanoparticles as antimicrobial components can not only control the antimicrobial components to have a better sustained-release effect, but also further enhance the antimicrobial effect, so that the hydrogel composition can continuously release the antimicrobial components for a long time and have a better effect; adopting a solvent method to prepare nano copper particles is simple and efficient. After obtaining a suspended matter solution, centrifugal treatment can separate the solid components in the suspended matter, washing treatment can remove impurities physically adsorbed on the surface of the particles, and drying treatment can obtain pure and dry nano copper particles, which are suitable for the subsequent preparation of a hydrogel composition.

[0022] In any of the above technical features, the mass ratio of 1,3,5-benzenetricarboxylic acid to the copper-containing compound is 1:2-3; and / or the rotation speed of the stirring treatment is 200rmp-300rmp; and / or the rotation speed of the centrifugal treatment is 8000rmp-10000rmp.

[0023] Compared with the prior art, the technical effects achieved by adopting the technical solution are as follows: adopting the above-mentioned ratio of 1,3,5-benzenetricarboxylic acid and the copper-containing compound can ensure that the reactants are fully contacted, make the reaction more complete, improve the yield, and make the size of the prepared nano copper particles uniform; adopting the above-mentioned rotation speed can ensure that the reactants are fully mixed while avoiding excessive reaction leading to particle agglomeration or uneven reaction; adopting the above-mentioned speed for centrifugal treatment can effectively separate the solid components in the suspension.

[0024] In any of the above technical features, between S100 and S200, the following is also included: S110, performing ultrasonic treatment on the polymer transparent liquid; The frequency of ultrasonic treatment is 20kHz-40kHz.

[0025] Compared with the prior art, the technical effect achieved by adopting this technical solution is as follows: when the polymer transparent liquid is subjected to ultrasonic treatment, when the ultrasonic wave propagates in the polymer transparent liquid, high-pressure and low-pressure areas of alternating compression and expansion are formed, and in the low-pressure area, tiny vacuum bubbles, i.e., cavitation bubbles, are generated in the polymer transparent liquid. These cavitation bubbles gradually increase in size during multiple pressure cycles and continuously absorb dissolved gases in the liquid. When these cavitation bubbles reach a certain size, they quickly rise to the surface of the polymer transparent liquid and burst, thereby releasing the gas therein, thereby removing bubbles in the polymer transparent liquid; when the above-mentioned frequency is used for ultrasonic treatment, the defoaming effect is usually the best and its structure will not be destroyed.

[0026] After adopting the technical solution of the present invention, the technical effects that can be achieved are as follows: 1. The hydrogel composition of the present invention introduces low-concentration antimicrobial components, and after the adhesive components form a viscous colloid, the antimicrobial components have more stable and effective bactericidal activity, can be more stably adhered to the wound, and the effective components are controlled to slowly seep out and act on the wound surface, without the need to add additional antibiotics, so that the hydrogel composition can have better antibacterial properties and promote wound healing. 2. Nano copper particles can achieve a balance between sustained antibacterial properties and biocompatibility. Introducing nano copper particles into hydrogel compositions can achieve excellent antibacterial properties while maintaining low cytotoxicity, which can be applied in the field of medical materials. 3. Using nanoparticles of benzyl alcohol, p-hydroxyacetophenone, sodium methylparaben, and sodium ethylparaben as antimicrobial components can not only control the antimicrobial components to have a better sustained-release effect, but also further enhance their antimicrobial effects, so that the hydrogel composition can continuously release the antimicrobial components for a longer period of time and have a better effect; 4. The preparation method of the present invention has a simple step, and the hydrogel composition can be prepared by only a simple stirring step. The antimicrobial component is added in two parts separately, which can further make the antimicrobial component evenly distributed in the prepared hydrogel composition. DETAILED DESCRIPTION

[0027] In order to more clearly understand the above-mentioned purpose, features and advantages of the present invention, the present invention is further described in detail below in conjunction with specific embodiments. It should be noted that the embodiments of the present application and the features in the embodiments can be combined with each other without conflict.

[0028] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Therefore, the protection scope of the present invention is not limited to the specific embodiments disclosed below.

[0029] In order to make the above-mentioned purposes, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below.

[0030] In the related art, antibiotics are usually added to antibacterial hydrogels. Although they have a certain antibacterial effect, their antibacterial components are often unable to be centrally processed after actual use. They are metabolized by the human body and then discarded into the environment, resulting in a certain concentration of residues. Various data show that the problem of microbial resistance caused by low-concentration antibiotic residues has become increasingly serious; moreover, the hydrogel materials that only have a physical coating effect in the current hydrogel products are in direct contact with body fluids, which can easily cause protein accumulation and microbial reproduction. Their own high water content also provides an effective living environment for the reproduction of microorganisms, which may increase the inflammatory response at the contact site.

[0031] In view of this, an embodiment of the present invention provides a hydrogel composition and a preparation method thereof, which introduces a low concentration of antimicrobial components, so that the antimicrobial components have more stable and effective bactericidal activity after the adhesive components form a viscous colloid, and can adhere to the wound more stably. By controlling the effective components to slowly seep out and act on the wound surface, the hydrogel composition can have better antibacterial properties and the function of promoting wound healing without the need to add additional antibiotics.

[0032] Specifically, an embodiment of the present invention provides a hydrogel composition, comprising: an adhesion component: 0.5wt.%-15wt.%, a moisturizing component: 0.5wt.%-10wt.%, a bioactive component: 0.01wt.%-5wt.%, a physical cross-linking agent: 0.01wt.%-5wt.%, a flavoring component: 0.01wt.%-2wt.%, an antimicrobial component: 0.01wt.%-2wt.%, medical purified water, and the rest; wherein the bioactive component includes at least one of lipoic acid, tea polyphenols, lactic acid bacteria extract and postbiotics; the antimicrobial component includes at least one of copper ions, benzyl alcohol, parahydroxyacetophenone, sodium methylparaben, and sodium ethylparaben.

[0033] Preferably, the hydrogel composition of the embodiment of the present invention comprises an adhesive component, a moisturizing component, a biological component, a physical crosslinking agent, a flavoring component, an antimicrobial component and medical purified water. By reasonably selecting an appropriate ratio of the adhesive component and the physical crosslinking agent, the adhesion ability of the hydrogel composition can be improved, so that the hydrogel composition can better adhere to the human skin. Under the action of the physical crosslinking agent, the adhesive component is cross-linked to form a network structure, which has better biocompatibility, is non-toxic or low-toxic, and is more suitable for use in the medical field.

[0034] Preferably, a wound surface that is kept moist can heal faster than a dry wound exposed to the air. The moist microenvironment provided by the hydrogel composition to the wound enables granulation tissue to grow faster, and the epithelial cells at the moist wound surface grow thicker and regenerate more. This microenvironment also alleviates the pain caused to the patient by the adhesion between the dressing and the wound surface, protects the nerve tissue of the wound surface, and promotes cell proliferation and capillary formation; in addition, the hydrogel dressing can absorb heat from the wound, thereby reducing the temperature of the wound surface in the early stage of wound formation, effectively inhibiting the occurrence of inflammation, slowing down nerve conduction, alleviating pain, promoting vasoconstriction, and reducing bleeding on the wound surface; therefore, the additional addition of a moisturizing component to the hydrogel composition can keep the hydrogel composition moist for a long time, further improving the healing speed of the wound.

[0035] Preferably, the presence of the flavoring ingredient can remove the odor in the hydrogel composition, making it more comfortable and pleasant during use.

[0036] Preferably, the bioactive ingredients bring antioxidant, anti-inflammatory and skin repair effects to the hydrogel composition, and the bioactive ingredients include at least one of lipoic acid, tea polyphenols, lactic acid bacteria extract and postbiotics, wherein lipoic acid can scavenge free radicals, reduce the negative impact of oxidative stress on wound healing, and promote the synthesis of collagen to enhance the skin's repair ability; tea polyphenols can inhibit inflammatory response, reduce redness and pain around the wound, and promote wound healing; lactic acid bacteria extract can regulate the acid-base balance of the skin, promote cell regeneration and metabolism, and increase the water content of the skin, keep the skin moist, and help wound healing; postbiotics can inhibit the growth of harmful bacteria and increase the number of beneficial bacteria, thereby improving the skin's immune function and repair ability.

[0037] Preferably, the antimicrobial component can promote wound healing by inhibiting the growth and reproduction of microorganisms and reducing the risk of infection. The antimicrobial component can provide an effective protective barrier to prevent bacteria and other microorganisms from entering the wound, and maintain a clean and moist environment for the wound, which is conducive to the smooth progress of the wound healing process. The antimicrobial component includes at least one of copper ions, benzyl alcohol, p-hydroxyacetophenone, sodium methylparaben, and sodium ethylparaben, wherein copper ions can destroy microbial cell walls and cell membranes, causing their death, and can also promote angiogenesis and tissue regeneration during wound healing; benzyl alcohol and p-hydroxyacetophenone can not only destroy microbial cell membranes, causing their death, but also promote tissue repair during wound healing; sodium methylparaben can effectively inhibit the growth of a variety of bacteria, it can not only destroy microbial cell membranes, causing their death, but also promote tissue repair during wound healing; sodium ethylparaben can destroy microbial cell membranes, causing their death.

[0038] Furthermore, the antimicrobial component includes at least one of nano copper particles and nano benzyl alcohol particles, nano p-hydroxyacetophenone particles, nano sodium methylhydroxybenzoate particles, and nano sodium ethylhydroxybenzoate particles.

[0039] Preferably, when copper ions are directly loaded on the temperature-responsive hydrogel, the free copper ions have a certain anti-polymerization effect, affecting the formation of the hydrogel network during the polymerization process, while the nano-copper particles can achieve a balance between sustained antibacterial properties and biocompatibility. Therefore, the nano-copper particles are introduced into the hydrogel composition to achieve excellent antibacterial properties while maintaining low cytotoxicity, which can be applied in the field of medical materials.

[0040] Preferably, nano-benzyl alcohol particles can generate reactive oxygen species (ROS) to damage bacterial cell structures. ROS can destroy bacterial cell membranes, proteins and genetic material, thereby quickly killing bacteria. Para-hydroxyacetophenone itself is widely used in the chemical, pharmaceutical and cosmetic industries due to its antioxidant, antibacterial and preservative activities. This compound is prepared into nanoparticles, which can be adsorbed on bacterial cell membranes, inhibit the activity of key enzymes in microbial cells, interfere with their metabolic processes, and further inhibit the growth and reproduction of microorganisms. When sodium methylparaben and sodium ethylparaben are made into nanoparticles, their specific surface area is greatly increased, which improves their contact efficiency with bacteria, thereby enhancing their antibacterial activity.

[0041] Preferably, nano-copper particles account for 75wt.%-90wt.% of all antimicrobial components. Nano-copper particles have more stable and effective bactericidal activity. The strong antibacterial properties can reduce the risk of cross-infection between wounds. A high proportion of nano-copper particles can ensure the continuous release of copper ions during wound healing and inhibit bacterial growth for a long time. In addition, nano-copper particles help to enhance the stability of the hydrogel, making it more suitable for wound application. Nano-copper particles can also increase the shelf life of the hydrogel, facilitating the long-term storage and transportation of the hydrogel composition.

[0042] Furthermore, the hydrogel composition also includes: a pH regulator: 0.01 wt.%-2 wt.%; the pH regulator is a buffer solution regulator.

[0043] Preferably, in the hydrogel composition, the ideal pH for copper ion release should be weakly acidic or neutral. Therefore, in order to optimize the antibacterial properties, stability and biocompatibility of the hydrogel composition, thereby ensuring its effectiveness and safety in practical applications, the hydrogel composition should also include a pH regulator; the use of the above-mentioned type of pH regulator helps to prevent undesirable chemical reactions caused by pH changes, thereby ensuring the chemical stability of the hydrogel, so that the pH value of the hydrogel composition can be stabilized within a range that is friendly to human cells, thereby reducing cytotoxicity and improving the biocompatibility of the hydrogel.

[0044] Preferably, the buffer solution is a phosphate buffer solution and an acetate buffer solution. The phosphate buffer solution has a wide buffering range and has good buffering capacity from acidic to alkaline, while the acetate buffer solution performs well mainly in the acidic range.

[0045] Preferably, the adhesive component includes at least one of chitosan, oligochitosan, carboxymethyl chitosan, chitosan acetate, polyvinyl alcohol, hydroxypropyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose, carbomer, sodium alginate, polyethylene glycol, and polyvinyl pyrrolidone. Chitosan, oligochitosan, carboxymethyl chitosan, and chitosan acetate have excellent biocompatibility and are suitable for various biomedical applications. They exhibit effective antibacterial and hemostatic abilities, can accelerate the wound healing process, and accelerate wound repair by promoting cell proliferation, migration, and angiogenesis, which is essential for preventing wound infection and promoting early hemostasis. Polyvinyl alcohol has good adhesion, can fit the wound closely, provide stable coverage, and help maintain a moist wound environment. Hydroxypropyl cellulose, carboxymethyl cellulose, and carboxyethyl cellulose all have excellent moisture retention and hygroscopicity, can provide a moist environment for the wound, and are beneficial to The invention relates to cell proliferation and ECM synthesis, and both of them have good biocompatibility and will not induce the body's rejection reaction; Carbomer can increase the viscosity and stability of the hydrogel composition, which helps the hydrogel composition to better cover and protect the wound, and can also help other components to be evenly dispersed in the hydrogel composition, ensuring that each part has the same activity and efficacy; Sodium alginate is a material with good biocompatibility and is suitable for long-term contact with the wound surface. It has good bioadhesion and can make the hydrogel polymer firmly adhere to the moist wound surface and is not easy to fall off; Polyethylene glycol and polyvinyl pyrrolidone can effectively reduce the adsorption of protein by the hydrogel and reduce the possibility of bacterial growth. Moreover, they can also help regulate the release rate of the active ingredients in the hydrogel composition, so that the drug can be released at the appropriate time and in an appropriate dose, thereby better promoting wound healing.

[0046] Preferably, the moisturizing component includes at least one of glycerol, propylene glycol, 1,2-hexanediol, and hyaluronic acid. Glycerol is a polyol compound with good biocompatibility and low toxicity as well as strong water absorption and water retention capacity. It is suitable for various types of wounds, including sensitive skin, and can effectively maintain a moist environment on the wound surface to prevent the wound from drying and scabbing. Propylene glycol has a certain antibacterial effect, which can inhibit wound infection and reduce bacterial growth. It not only has a moisturizing function, but also can enhance the permeability of other ingredients, so that the active ingredients in the hydrogel can play a better role. 1,2-hexanediol has both moisturizing and antibacterial properties, which can effectively inhibit wound infection and provide a moist environment for the wound surface. This ingredient is basically non-irritating to the skin, suitable for long-term use, and reduces secondary damage to the wound surface. Hyaluronic acid has super strong water absorption and water retention capacity, which can provide an ideal moist environment for the wound surface, accelerate cell proliferation and migration, and promote wound healing. Hyaluronic acid also has an anti-inflammatory effect, which can regulate the inflammatory response of the wound surface, promote the transition of chronic wounds to the proliferation stage, and accelerate wound healing.

[0047] Preferably, the physical crosslinking agent includes at least one of calcium chloride, calcium carbonate, sodium sulfate, sodium tripolyphosphate, and citric acid. Calcium chloride can improve the compressive strength and modulus of the hydrogel through ionic crosslinking, so that it can fit the wound more stably; calcium carbonate will produce a slightly alkaline environment when dissolved, which is beneficial to the healing process of certain wounds. The hydrogel crosslinked by calcium carbonate has better compressive resistance and reduces damage caused by external forces; sodium sulfate can help the hydrogel maintain appropriate moisture and avoid wound drying. By controlling the concentration of sodium sulfate, the swelling behavior of the hydrogel in different environments can be adjusted to adapt to different wound types; sodium tripolyphosphate can effectively crosslink a variety of polymers and enhance the network structure of the hydrogel. The phosphate ions released by sodium tripolyphosphate can participate in the energy metabolism of cells, promote cell proliferation and migration, and accelerate wound repair; the addition of citric acid can improve the flexibility of the hydrogel, so that it can fit more comfortably on irregular wounds.

[0048] Preferably, the flavoring component includes at least one of a sweetener, menthol, and a flavoring agent, which can improve the taste and smell of the hydrogel composition and enhance the comfort of use for patients. Certain flavoring components, such as menthol, also have a cooling and antipruritic effect and can relieve itching symptoms on the wound surface.

[0049] An embodiment of the present invention further provides a method for preparing a hydrogel composition, which is used to prepare a hydrogel composition according to any one of the above technical features, and the preparation method comprises: S100, adding the adhesive component and the physical cross-linking agent into medical purified water, stirring evenly, then adding part of the antimicrobial component, and continuing to stir evenly to obtain a polymer transparent liquid; S200, adding the moisturizing component, the bioactive component, the flavor correcting component and the remaining antimicrobial component into the polymer transparent liquid, stirring evenly to obtain a hydrogel composition.

[0050] Preferably, the mass ratio of the adhesive component, the physical cross-linking agent and the medical purified water is 0.1-3: 0.002-1: 0.2-20; and / or the mass ratio of the moisturizing component, the bioactive component and the flavoring component is 0.1-2: 0.002-1: 0.002-0.4; and / or the mass ratio of the antimicrobial component and the medical purified water is 0.002-0.4: 0.2-20; wherein the antimicrobial component comprises at least one of copper ions, benzyl alcohol, p-hydroxyacetophenone, sodium methylparaben and sodium ethylparaben.

[0051] Preferably, the preparation method of the present invention fully considers the performance characteristics of the hydrogel and the needs of wound healing, and ensures the effectiveness and safety of the product by adding ingredients with different functions step by step. By designing the dosage ratio between the components of the hydrogel composition, the prepared hydrogel composition can effectively adhere to the wound surface, which can not only maintain the moist environment of the wound and promote cell growth and migration, but also promote cell proliferation and differentiation while providing effective antibacterial protection, thereby ensuring the safety and effectiveness of the hydrogel composition in clinical applications.

[0052] Preferably, in S100, the stirring time is 10 h-24 h, and before S200, the polymer transparent liquid should be cooled to 25° C., and the stirring time in S200 is 2 h.

[0053] Furthermore, the antimicrobial component includes nano copper particles and at least one of nano benzyl alcohol particles, nano p-hydroxyacetophenone particles, nano sodium methylhydroxybenzoate particles, and nano sodium ethylhydroxybenzoate particles; wherein the preparation method of the nano copper particles includes: S101, adding a copper-containing compound to an ethanol solution of 1,3,5-benzenetricarboxylic acid, stirring the mixture, and obtaining a suspension solution; S102, sequentially centrifuging, washing and drying the suspended solution to obtain nano copper particles.

[0054] Preferably, nano copper particles and at least one of nano benzyl alcohol particles, nano p-hydroxyacetophenone particles, nano sodium methylhydroxybenzoate particles, and nano sodium ethylhydroxybenzoate particles are used as the antimicrobial component, which can not only control the antimicrobial component to have a better sustained release effect, but also further enhance its antimicrobial effect, so that the hydrogel composition can continuously release the antimicrobial component for a long time and have a better effect; the solvent method is used to prepare the nano copper particles, which is simple and efficient. After obtaining the suspension solution, centrifugal treatment can separate the solid components in the suspension, washing treatment can remove impurities physically adsorbed on the surface of the particles, and drying treatment can obtain pure and dry nano copper particles, which are suitable for the subsequent preparation of the hydrogel composition.

[0055] Preferably, after the stirring treatment in S200 is completed, ultrasonic treatment and irradiation treatment should be further performed, the frequency of the ultrasonic treatment is 20kHz-40kHz, and the time is 15min-30min, so as to eliminate bubbles generated during the stirring treatment; the irradiation treatment is preferably ultraviolet irradiation treatment, the ultraviolet irradiation intensity is 100μW / cm2-150μW / cm2, and the irradiation time is 15min-30min, which can further sterilize the hydrogel composition.

[0056] Preferably, in S101, part of the copper compound is first added to the ethanol solution of 1,3,5-benzenetricarboxylic acid, and then stirred. During the stirring process, the remaining copper compound is continuously added, and the stirring time is preferably 25min-35min; in S102, ethanol is preferably used for washing, and the washing treatment is preferably carried out three times. The temperature of the drying treatment is preferably 65°C-85°C, and the time is preferably 1h-2h.

[0057] Preferably, the mass ratio of 1,3,5-benzenetricarboxylic acid to the copper-containing compound is 1:2-3; and / or the rotation speed of the stirring treatment is 200 rpm-300 rpm; and / or the rotation speed of the centrifugal treatment is 8000 rpm-10000 rpm.

[0058] Preferably, the mass ratio of 1,3,5-benzenetricarboxylic acid to the copper-containing compound is 1:2-3, which can ensure that the reactants are fully contacted, make the reaction more complete, improve the yield, and make the size of the prepared nano-copper particles uniform; the stirring speed is 200rmp-300rmp, which can ensure that the reactants are fully mixed while avoiding excessive reaction leading to particle agglomeration or uneven reaction; the centrifugal speed is 8000rmp-10000rmp, and the time is preferably 30min-45min, which can effectively separate the solid components in the suspension.

[0059] Preferably, between S100 and S200, the following is also included: S110, performing ultrasonic treatment on the polymer transparent liquid; The frequency of ultrasonic treatment is 20kHz-40kHz.

[0060] Preferably, the polymer transparent liquid is subjected to ultrasonic treatment. When ultrasonic waves propagate in the polymer transparent liquid, high-pressure and low-pressure areas of alternating compression and expansion are formed. In the low-pressure area, tiny vacuum bubbles, i.e., cavitation bubbles, are generated in the polymer transparent liquid. These cavitation bubbles gradually increase in multiple pressure cycles and continuously absorb dissolved gases in the liquid. When these cavitation bubbles reach a certain size, they quickly rise to the surface of the polymer transparent liquid and burst, thereby releasing the gas therein, thereby removing bubbles in the polymer transparent liquid. When the frequency of the ultrasonic treatment is 20kHz-40kHz, the time of the ultrasonic treatment is preferably 15min-30min, and the defoaming effect is usually the best without destroying its structure.

[0061] [Example 1] The embodiment of the present invention provides a hydrogel composition, including an adhesive component of sodium alginate 0.2 wt.%, carboxymethyl chitosan 0.3 wt.%, a moisturizing component of glycerol 0.5 wt.%, a physical stabilizer of calcium chloride 0.01 wt.%, a flavoring component of menthol 0.01 wt.%, a biologically active component of lipoic acid 2 wt.%, an antimicrobial component of copper ions 1.5 wt.%, benzyl alcohol 0.5 wt.%, and medical purified water 94.98 wt.%; The preparation method of the hydrogel composition comprises: S100, adding sodium alginate, carboxymethyl chitosan and calcium chloride into medical purified water, stirring for 10 hours, then adding copper ions, and continuing to stir evenly to obtain a polymer transparent liquid; S200, lowering the temperature of the polymer transparent liquid to 25°C, adding glycerol, lipoic acid, menthol and benzyl alcohol to the polymer transparent liquid, and stirring for 2 hours to obtain a hydrogel composition.

[0062] [Example 2] The embodiment of the present invention provides a hydrogel composition, including an adhesive component of sodium alginate 2wt.%, polyvinyl alcohol 8wt.%, carboxymethyl chitosan 5wt.%, a moisturizing component of glycerol 10wt.%, a physical stabilizer of calcium chloride 2wt.%, a flavoring component of menthol 0.01wt.%, a biologically active component of lipoic acid 0.01wt.%, an antimicrobial component of copper ion 0.009wt.%, benzyl alcohol 0.001wt.%, and medical purified water 72.97wt.%; The preparation method of the hydrogel composition comprises: S100, adding sodium alginate, carboxymethyl chitosan and calcium chloride into medical purified water, stirring for 10 hours, then adding copper ions, continuing to stir evenly to obtain a polymer transparent liquid, and then subjecting the polymer transparent liquid to ultrasonic treatment at a frequency of 20 kHz and a time of 30 minutes; S200, lowering the temperature of the polymer transparent liquid to 25°C, adding glycerol, lipoic acid, menthol and benzyl alcohol to the polymer transparent liquid, stirring for 2 hours, ultrasonically treating at 20kHz for 30 minutes, and then irradiating under 100μW / cm2 ultraviolet rays for 30 minutes to obtain a hydrogel composition.

[0063] [Example 3] The embodiment of the present invention provides a hydrogel composition, including 8wt.% of carbomer as an adhesive component, 0.5% of polyvinyl alcohol, 1wt.% of vinyl pyrrolidone, 3wt.% of propylene glycol as a moisturizing component, 6wt.% of 1,2-hexanediol as a physical stabilizer, 1wt.% of calcium chloride as a flavoring component, 0.01wt.% of menthol as a flavoring component, 2wt.% of lipoic acid as a biologically active component, 1.8wt.% of nano copper particles as an antimicrobial component, 0.02wt.% of nano benzyl alcohol particles, and 76.49wt.% of medical purified water; The preparation method of the hydrogel composition comprises: S100, adding carbomer, polyvinyl alcohol, vinyl pyrrolidone and calcium chloride into medical purified water, stirring evenly, then adding nano copper particles, stirring for 12 hours to obtain a polymer transparent liquid, and then subjecting the polymer transparent liquid to ultrasonic treatment at a frequency of 40 kHz and a time of 15 minutes; S200, lowering the temperature of the polymer transparent liquid to 25°C, adding propylene glycol and 1,2-hexanediol, lipoic acid, menthol and nano-benzyl alcohol particles to the polymer transparent liquid, stirring for 2 hours, ultrasonically treating at 40kHz for 15 minutes, and then irradiating under 150μW / cm2 ultraviolet rays for 15 minutes to obtain a hydrogel composition; Among them, the nano copper particles are purchased nano copper particles.

[0064] [Example 4] The embodiment of the present invention provides a hydrogel composition, including 10wt.% of polyvinyl alcohol as an adhesive component, 3% of chitosan hydrochloride, 6wt.% of propylene glycol as a moisturizing component, 0.02wt.% of calcium chloride as a physical stabilizer, 0.01wt.% of menthol as a flavoring component, 0.5wt.% of lipoic acid as a biologically active component, 0.17wt.% of nano copper particles as an antimicrobial component, 0.04wt.% of nano benzyl alcohol particles, and 80.27wt.% of medical purified water; the preparation method of the hydrogel composition includes: S100, adding carbomer, polyvinyl alcohol, vinyl pyrrolidone and calcium chloride into medical purified water, stirring evenly, then adding nano copper particles, continuing stirring for 24 hours to obtain a polymer transparent liquid, and then subjecting the polymer transparent liquid to ultrasonic treatment at a frequency of 30 kHz and a time of 20 minutes; S200, lowering the temperature of the polymer transparent liquid to 25°C, adding propylene glycol and 1,2-hexanediol, lipoic acid, menthol and nano-benzyl alcohol particles to the polymer transparent liquid, stirring for 2 hours, ultrasonically treating at 30kHz for 20 minutes, and then irradiating at 100μW / cm2 ultraviolet rays for 20 minutes to obtain a hydrogel composition; The preparation method of nano copper particles includes: S101, adding part of the copper acetate monohydrate to the ethanol solution of 1,3,5-benzenetricarboxylic acid, stirring for 35 minutes, and adding the remaining copper acetate monohydrate during the stirring process to obtain a suspension solution; S102. The suspended solution was centrifuged at 8000 rpm for 45 min, then washed three times with 75% ethanol, and finally dried at 65° C. for 2 h to obtain nano copper particles.

[0065] [Example 5] The embodiment of the present invention provides a hydrogel composition, including 10wt.% of polyvinyl alcohol as an adhesive component, 3% of chitosan hydrochloride, 2wt.% of oligochitosan, 6wt.% of propylene glycol as a moisturizing component, 0.02wt.% of hyaluronic acid, 0.02wt.% of calcium chloride as a physical stabilizer, 0.01wt.% of menthol as a flavoring component, 0.5wt.% of lipoic acid as a biologically active component, 0.17wt.% of nano copper particles as an antimicrobial component, 0.04wt.% of nano benzyl alcohol particles, and 78.74wt.% of medical purified water; the preparation method of the hydrogel composition includes: S100, adding carbomer, polyvinyl alcohol, vinyl pyrrolidone and calcium chloride into medical purified water, stirring evenly, then adding nano copper particles, stirring for 15 hours to obtain a polymer transparent liquid, and then subjecting the polymer transparent liquid to ultrasonic treatment at a frequency of 30 kHz and a time of 25 minutes; S200, lowering the temperature of the polymer transparent liquid to 25°C, adding propylene glycol and 1,2-hexanediol, lipoic acid, menthol and nano-benzyl alcohol particles to the polymer transparent liquid, stirring for 2 hours, ultrasonically treating at 30kHz for 25 minutes, and then irradiating under 110μW / cm2 ultraviolet rays for 20 minutes to obtain a hydrogel composition; The preparation method of nano copper particles includes: S101, adding part of the copper acetate monohydrate to the ethanol solution of 1,3,5-benzenetricarboxylic acid, stirring for 45 minutes, and adding the remaining copper acetate monohydrate during the stirring process to obtain a suspension solution; S102. The suspended solution was centrifuged at 10,000 rpm for 45 minutes, then washed with 75% ethanol for three times, and finally dried at 85° C. for 1 hour to obtain nano copper particles.

[0066]

Performance test

[0067] The adhesion time of the hydrogel polymer in Example 1 is 42 min, the adhesion time of the hydrogel polymer in Example 2 is 53 min, the adhesion time of the hydrogel polymer in Example 3 is 59 min, the adhesion time of the hydrogel polymer in Example 4 is 72 min, and the adhesion time of the hydrogel polymer in Example 5 is 87 min.

[0068] Test 2: Animal testing Take 50 rats weighing 200-300g, feed them in standard cages, and allow free drinking water and diet. Set a 15-day transition period. Prepare a wound with an area of ​​220mm2 and a depth of 2mm on the back. Set up a blank control experiment for each group of example gels. All hydrogel polymers in Examples 1 to 5 are applied to the wound in the form of external coating. The control group is rats that did not receive any treatment as the control group, and the test time is 21 days. The wounds of the rats in the gel group of the example group began to heal to varying degrees after 2 days, but the wound healing of the experimental group was accelerated to varying degrees in the period after 4 to 8 days. It shows that the hydrogel in the example has the effect of accelerating wound healing.

[0069] Test 3: Sample placement stability test Sample test after 18 months (sampling the change of single ingredient content) The components of glycerol, propylene glycol, 1,2-hexanediol, and p-hydroxyacetophenone in the gel were tested using NMR, GC-MS, MS, and LC-MSMS methods to test their content stability and distribution uniformity (whether they were stratified). The test of single components of glycerol, propylene glycol, 1,2-hexanediol, and p-hydroxyacetophenone in the gel showed that the stability results of the samples were good after 18 months of storage, and the quality was controllable.

[0070] In the description of this specification, the description of the terms "one embodiment", "some embodiments", "specific embodiments", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0071] Although the present invention is disclosed as above, the present invention is not limited thereto. Any person skilled in the art can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the scope defined by the claims.

Claims

1. A hydrogel composition, characterized in that: include: Adhesion component: 0.5wt.%-15wt.%, Moisturizing ingredients: 0.5wt.%-10wt.%, Bioactive ingredients: 0.01wt.%-5wt.%, Physical crosslinking agent: 0.01wt.%-5wt.%, Flavor ingredients: 0.01wt.%-2wt.%, Antimicrobial ingredients: 0.01wt.%-2wt.%, Medical purified water, surplus; Wherein, the bioactive ingredients include at least one of lipoic acid, tea polyphenols, lactic acid bacteria extract and postbiotics; The antimicrobial component includes at least one of copper ions, benzyl alcohol, p-hydroxyacetophenone, sodium methylparaben, and sodium ethylparaben; The preparation method of the hydrogel composition comprises: S100, adding the adhesive component and the physical crosslinking agent into the medical purified water, stirring evenly, then adding part of the antimicrobial component, and continuing to stir evenly to obtain a polymer transparent liquid; S200, adding the moisturizing component, the bioactive component, the flavoring component and the remaining antimicrobial component into the polymer transparent liquid, stirring evenly to obtain the hydrogel composition.

2. The hydrogel composition according to claim 1, characterized in that The antimicrobial component comprises at least one of nano copper particles and nano benzyl alcohol particles, nano p-hydroxyacetophenone particles, nano sodium methylhydroxybenzoate particles and nano sodium ethylhydroxybenzoate particles.

3. The hydrogel composition according to claim 2, characterized in that The nano copper particles account for 75wt.%-90wt.% of the entire antimicrobial component.

4. The hydrogel composition according to claim 1, characterized in that The hydrogel composition further comprises: pH adjuster: 0.01wt.%-2wt.%; The pH regulator is a buffer solution regulator.

5. The hydrogel composition according to claim 1, characterized in that The adhesive component comprises at least one of chitosan, oligochitosan, carboxymethyl chitosan, chitosan acetate, polyvinyl alcohol, hydroxypropyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose, carbomer, sodium alginate, polyethylene glycol, and polyvinyl pyrrolidone; and / or The moisturizing ingredient includes at least one of glycerin, propylene glycol, 1,2-hexanediol, and hyaluronic acid; and / or The physical crosslinking agent comprises at least one of calcium chloride, calcium carbonate, sodium sulfate, sodium tripolyphosphate, and citric acid; and / or The flavoring component includes at least one of a sweetener, menthol, and a flavoring agent.

6. A method for preparing a hydrogel composition, characterized in that: For preparing a hydrogel composition as claimed in any one of claims 1 to 5, the preparation method comprising: S100, adding the adhesive component and the physical crosslinking agent into the medical purified water, stirring evenly, then adding part of the antimicrobial component, and continuing to stir evenly to obtain a polymer transparent liquid; S200, adding the moisturizing component, the bioactive component, the flavoring component and the remaining antimicrobial component into the polymer transparent liquid, stirring evenly to obtain the hydrogel composition.

7. The preparation method according to claim 6, characterized in that The mass ratio of the adhesive component, the physical cross-linking agent and the medical purified water is 0.1-3: 0.002-1: 0.2-20; and / or The mass ratio of the moisturizing component, the bioactive component and the flavoring component is 0.1-2: 0.002-1: 0.002-0.4; and / or The mass ratio of the antimicrobial component to the medical purified water is 0.002-0.4:0.2-20; Wherein, the antimicrobial component includes at least one of copper ions, benzyl alcohol, p-hydroxyacetophenone, sodium methylparaben, and sodium ethylparaben.

8. The preparation method according to claim 7, characterized in that The antimicrobial component comprises at least one of nano copper particles and nano benzyl alcohol particles, nano p-hydroxyacetophenone particles, nano sodium methylhydroxybenzoate particles, and nano sodium ethylhydroxybenzoate particles; Wherein, the preparation method of the nano copper particles comprises: S101, adding a copper-containing compound to an ethanol solution of 1,3,5-benzenetricarboxylic acid, stirring the mixture, and obtaining a suspension solution; S102, sequentially centrifuging, washing, and drying the suspended solution to obtain the nano copper particles.

9. The preparation method according to claim 8, characterized in that The mass ratio of the 1,3,5-benzenetricarboxylic acid to the copper-containing compound is 1:2-3; and / or The stirring speed is 200-300 rpm; and / or The rotation speed of the centrifugal treatment is 8000 rpm-10000 rpm.

10. The preparation method according to claim 6, characterized in that Also included between S100 and S200: S110, subjecting the polymer transparent liquid to ultrasonic treatment; The frequency of the ultrasonic treatment is 20kHz-40kHz.