Combined water-soluble fertilizer containing plant immune activator protein and seaweed and preparation method of combined water-soluble fertilizer

Abstract

The invention discloses a combined water-soluble fertilizer containing plant immune activator protein and seaweed and a preparation method, and relates to the technical field of chemical fertilizer preparation, the combined water-soluble fertilizer comprises the following components by mass: 50-70 parts of a seaweed mother liquor, 20-30 parts of a plant immune activator protein mother liquor (containing a protein stabilizer), and 5-15 parts of an organic matter, the organic matter comprises one or more compositions of glucose, glycerol or sugar alcohols. The natural organic seaweed fertilizer and the plant immune activator protein are reasonably proportioned, and the functions and characteristics of the natural organic seaweed fertilizer and the plant immune activator protein are fused together, so that on one hand, organic raw materials provided by the seaweed can promote the plant immune activator protein to better exert the immune activity of the plant immune activator protein; on the other hand, the plant immune activator protein can actively mobilize the absorption and utilization of the seaweed fertilizer by plants, and the purposes of reducing the use of the fertilizer, increasing the yield and improving the quality of crops are achieved.

Description

Technical Field

[0001] The invention relates to the technical field of fertilizer preparation, and in particular to a water-soluble fertilizer composed of plant immune activating protein and seaweed and a preparation method thereof. Technical Background

[0002] During the long process of biological evolution, a very complex relationship has been established between plants and microorganisms. Plants defend against the invasion of pathogenic microorganisms and establish a complex and complete defense mechanism. At the same time, pathogenic microorganisms are gradually adapting to the defense mechanism of plants. The plant immune activation protein Peat1 is a protein isolated from Alternaria tenuissima by Qiu Dewen and others. It has been proven to have significant growth-promoting and resistance-inducing effects. This protein can act on plants and induce plants to produce defense responses, trigger the plant's own immune defense system to take effect, comprehensively improve the plant's own metabolic level, enhance the ability to defend against pests and natural stresses, and thus increase crop yield and quality. The protein is used in combination with amino oligosaccharides to develop the plant immune inducer Ata Ling, which has been well received in the market and has successfully gone abroad. Ata Ling enhances plant disease resistance by activating the plant defense system, while enhancing plant photosynthesis, respiration, etc., thereby increasing the yield and quality of crops. In addition, Ateling can increase the emergence rate and thousand-grain weight of oats and enhance disease resistance, increasing the yield of oats per unit area by 17.80%; it can also increase the starch content of wheat; when used on grapes, in addition to having a disease prevention effect, it can also promote the growth of grape stems and leaves, increase the soluble solids content, and increase sugar content. In short, plant immune activation proteins are increasingly used in grain crops, fruits, vegetables and other crops. They not only have good disease prevention effects, but also show advantages that cannot be ignored in increasing crop yields and improving quality. However, there are very few studies and products on the application of plant immune activation proteins in crop fertilizers.

[0003] Seaweed is the main producer of organic compounds, with the characteristics of fast growth and strong adaptability. It is the largest and most renewable biological resource in the ocean, with more than 10,000 species, accounting for 10% of the ocean's productivity. In order to cope with special environmental stresses, seaweed has accumulated more than ten components including seaweed polysaccharides, seaweed polyphenols, mannitol, betaine, plant hormones, cytochromes, etc. Seaweed extracts can be made into new organic fertilizers after scientific processing to break the cell walls and release the contents. As a biostimulant for agricultural production, it is of great significance to regulate the growth and development of crops. Compared with traditional chemical fertilizers, it has the advantages of safety, non-toxicity, green environmental protection, and low production cost. It has been widely used to promote nutrient absorption, improve plant resistance, and improve plant quality. It is the fourth fertilizer after organic fertilizers, chemical fertilizers, and biological fertilizers. Seaweed has been made into fertilizer for thousands of years. Compared with developed countries in Europe and the United States, our country started late in the industrial production and development research of seaweed fertilizer. Although it has developed rapidly, domestic seaweed fertilizer products are relatively stable and the market prospects for seaweed fertilizer are very promising. However, our application areas of seaweed fertilizer are relatively small, and the potential for the rational use of seaweed fertilizer needs to be further fully explored.

[0004] Among the existing technologies, the production process of seaweed fertilizer at home and abroad is mature, but there are still problems such as incomplete degradation of the main components, insignificant synergistic effect when mixed with chemical fertilizers, low crop absorption and utilization rate, slow effect, etc., which lead to excessive fertilization, soil salinization, excessive heavy metals and economic losses. To this end, a combined water-soluble fertilizer of plant immune activation protein and seaweed and a preparation method are proposed. Summary of the invention

[0005] The purpose of the present invention is to provide a combined water-soluble fertilizer containing plant immune activating protein and seaweed and a preparation method thereof, which has few types of raw materials, simple preparation process and good product stability.

[0006] In order to solve the above technical problems, the technical solution adopted by the present invention is as follows: a combined water-soluble fertilizer containing plant immune activating protein and seaweed, characterized in that it comprises, by mass percentage: 50-70 parts of seaweed mother solution, 20-30 parts of plant immune activating protein mother solution (containing protein stabilizer), and 5-15 parts of organic matter, wherein the organic matter includes one or more combinations of glucose, glycerol or sugar alcohols.

[0007] A method for preparing a combined water-soluble fertilizer containing plant immune activating protein and seaweed, characterized in that it comprises the following steps: a. Seed activation and culture: Take out the seed solution from the -80℃ refrigerator, thaw and activate it, and then inoculate it into the culture medium for culture; b. Access the above seed solution to the primary fermentation tank, culture for 3 hours, and pressurize the air tank to a 5000L fermentation tank; c. Access to the above 500L fermentation tank seed solution culture to the secondary fermentation tank fermentation, the protein mother liquor, spare; d. Premix 50-70 parts of seaweed mother liquid with 5-15 parts of organic matter for later use; e. Add 20-30 parts of protein mother solution (including protein stabilizer) to the above seaweed organic matter premix to obtain a finished product of seaweed combined water-soluble fertilizer containing plant immune activating protein.

[0008] Preferably, in step a, the culture medium components include tryptone, yeast extract powder, sodium chloride and purified water, which are sterilized by wet heat sterilization at 150° C. for 50 min and cooled to 25° C. for standby use.

[0009] Preferably, the specific steps of the secondary fermentation in step c are: weighing the secondary fermentation raw materials and feeding them into a 5000L tank, stirring to dissolve, sterilizing with wet heat at 150°C for 50 minutes, and then adding the seed liquid of the 500L fermentation tank after cooling to start culturing; adjusting the pH with alkaline solution, adding a defoamer to control the foam, and supplementing sugar. After the fermentation is completed, the tank is released to obtain a protein mother liquor.

[0010] Preferably, the specific steps in step e are: stirring continuously for 3-4 hours after premixing, adding acid to adjust the pH to 2-4 and then placing in a tank for sedimentation for 48 hours, filtering with a 50 μm filter to obtain a finished combined water-soluble fertilizer, wherein the content of plant immune activating protein is 0.05-0.25 g / L.

[0011] The beneficial effects of the present invention are: 1. The unique effects of seaweed and plant immune activation protein combine their functions and characteristics to enhance each other's effects, and finally become a complementary synergistic combination of water-soluble fertilizers, achieving the goal of reducing fertilizer use, increasing crop yields and improving quality; 2. On the basis of exerting its inherent advantages, the organic raw materials provided by seaweed can also promote plant immune activation protein to better exert its immune activity; 3. After binding to the membrane protein on the surface of crop leaves, plant immune activation protein can cause the increase of various physiological indicators of crops, enhance the absorption and utilization rate of seaweed fertilizer by plants, and then better promote plant development, reduce economic losses and soil salinization problems caused by excessive use of seaweed fertilizer; 4. The combined water-soluble fertilizer containing plant immune activating protein and seaweed of the present invention, the organic raw materials provided by the seaweed can promote the plant immune activating protein to better exert its immune activity, and at the same time, the plant immune activating protein can enhance the plant's absorption and utilization rate of seaweed fertilizer, and shows a significant improvement effect on tomato yield and blueberry quality (vitamin C, anthocyanin). BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1It is a flow chart of the preparation method of the water-soluble fertilizer containing plant immune activating protein and seaweed of the present invention; DETAILED DESCRIPTION

[0013] To make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described in detail below. The following application examples are convenient for better understanding of the present invention, but do not limit the present invention. The experimental methods in the following application examples are conventional methods unless otherwise specified.

[0014] The present invention provides a seaweed combined water-soluble fertilizer containing plant immune activating protein, which comprises, by mass percentage, 50-70 parts of seaweed mother solution, 20-30 parts of plant immune activating protein mother solution (containing protein stabilizer), and 5-15 parts of organic matter, wherein the organic matter comprises one or more combinations of glucose, glycerol or sugar alcohols.

[0015] The principles of the ratio of the seaweed combined water-soluble fertilizer containing plant immune activating protein of the present invention are: 1. Seaweed provides abundant natural nutrients for crops, including carbohydrates, plant growth regulators, vitamins and minerals; 2. The plant immune activating protein has the best effect when used at 0.05-0.25g / L; 3. Organic matter is used as a nutrient supplement for the insufficient seaweed, providing a precise and reasonable nutritional ratio for crops. The above-mentioned organic matter is one or more compositions including glucose, glycerol or sugar alcohols.

[0016] See attached Figure 1 Based on the above-mentioned seaweed combination water-soluble fertilizer containing plant immune activating protein, the present invention also provides a method for preparing the seaweed combination water-soluble fertilizer containing plant immune activating protein, comprising the following steps.

[0017] a. Seed activation: Take out the seed solution from the -80℃ refrigerator, thaw and activate it, and then transfer it to the shake flask; b. Inoculation and shake flask culture: Preparation of culture medium: dissolve tryptone, yeast extract powder, sodium chloride and purified water in bottles, sterilize at 150°C for 50 minutes, cool to 25°C, inoculate seeds on a clean bench, shake the flask overnight on a constant temperature shaking bed, and pour the seed solution into the clean bench; c. 500L seed culture tank: accurately weigh the primary fermentation raw materials and put them into a 500L tank, stir to dissolve, sterilize at 150℃ for 50min, insert the seed liquid through the needle, start culturing for 3 hours, and pressurize the tank into a 5000L fermentation tank with air; d. 5000L fermentation tank culture: Fermentation raw material preparation: accurately weigh the secondary fermentation raw materials and feed them into the 5000L tank, stir to dissolve, sterilize at 150℃ for 50min, cool down and add the 500L fermentation tank seed liquid to start culture. Use alkali solution to adjust pH. Add defoamer to control foam. After stirring and dissolving, sterilize at 150℃ for 30 minutes, cool down and add sugar to the 5000L fermentation tank, and release the tank after fermentation to obtain protein mother liquor; Application Example 1: This example shows the application effect on blueberries

[0018] Application of seaweed combination water-soluble fertilizer containing plant immune activating protein and blueberry finished product: add 60 parts of seaweed mother liquor in proportion in the preparation tank, add 25 parts of protein mother liquor (containing protein stabilizer) after 15 parts of organic matter are dissolved, and stir continuously for 4 hours, add acid to adjust pH 4, and then put it in the tank for sedimentation for 48 hours, filter with a 50μm filter, take samples for inspection, and package them according to different specifications after passing the inspection, which is the finished product of protein seaweed fertilizer water with a protein content of 0.25g / L; wherein the organic matter is one or more combinations of glucose, glycerol or sugar alcohols.

[0019] In this example, during the blooming period to the fruiting period of blueberry growth, i.e., from April to June, the combined water-soluble fertilizer containing plant immune activating protein and seaweed of the present invention was diluted according to the experimental group of 1:200 times: 1:300 times: 1:400 times: 1:500 times: 1:600 ​​times, and then drip irrigation was performed on blueberries. Each treatment was 20 plants, and drip irrigation was performed once every 10 days, for a total of 3 times. The application effect of the combined water-soluble fertilizer on blueberries was investigated 10-15 days after the last drip irrigation. The control group was water, a single dose of plant immune activating protein diluted 1:300 times, a single dose of seaweed diluted 1:300 times, and Ata Ling diluted 1:300 times. After three sprayings of the combined water-soluble fertilizer containing plant immune activating protein and seaweed, the combined water-soluble fertilizer diluted 1:200 times, 1:300 times, and 1:400 times had a significant effect on improving the vitamin C and anthocyanin content of blueberry fruit seeds. The results are as follows: Table 1 Effect of application of combined water-soluble fertilizer containing plant immune activating protein and seaweed on blueberry

[0020] Application Example 2: This example shows the application effect on tomatoes

[0021] The seaweed combination water-soluble fertilizer containing plant immune activating protein is applied to tomato finished products: 70 parts of seaweed mother liquor are added in proportion in the preparation tank, 15 parts of organic matter are dissolved, and then 15 parts of protein mother liquor (containing protein stabilizer) are added and stirred continuously for 4 hours, acid is added to adjust the pH to 4, and then the tank is placed for sedimentation for 48 hours, filtered with a 50μm filter, samples are taken for inspection, and those that pass the inspection are packaged according to different specifications, which is a finished product of protein seaweed fertilizer water with a protein content of 0.15g / L; wherein the organic matter is one or more combinations of glucose, glycerol or sugar alcohols.

[0022] In this example, the test crop is tomato. From the seedling stage to the flowering stage of tomato growth, that is, from April to June, foliar fertilization is applied to tomatoes, sprayed once every 10 days, for a total of three times, and the yield is measured after the fruit matures. The combined water-soluble fertilizer containing plant immune activating protein and seaweed of the present invention is diluted by 1:200 times: 1:300 times: 1:400 times: 1:500 times: 1:600 ​​times according to the experimental group, and then sprayed on tomatoes; the control group is water, a single dose of plant immune activating protein diluted 1:300 times, a single dose of seaweed diluted 1:300 times, and Ata Ling diluted 1:300 times. After three sprayings of the combined water-soluble fertilizer containing plant immune activating protein and seaweed, the combined water-soluble fertilizer diluted 1:200 to 600 times has a significant yield-increasing effect on tomato yield, among which the combined water-soluble fertilizer diluted 1:300 times and 1:400 times has the best effect, and the results are as shown in the following table: Table 2 Effect of combined water-soluble fertilizer containing plant immune activating protein and seaweed on tomatoes

Claims

1. A combined water-soluble fertilizer containing plant immune activating protein and seaweed, characterized in that: The composition comprises, by mass percentage, 50-70 parts of seaweed mother solution, 20-30 parts of plant immune activating protein mother solution (including protein stabilizer), and 5-15 parts of organic matter.

2. A combined water-soluble fertilizer containing plant immune activating protein and seaweed according to claim 1, characterized in that: The organic matter includes one or more combinations of glucose, glycerol or sugar alcohols.

3. A method for preparing a combined water-soluble fertilizer containing plant immune activating protein and seaweed according to any one of claims 1-2, characterized in that, The following steps are involved: a. Seed activation and culture: Take out the seed solution from the -80℃ refrigerator, thaw and activate it, and then inoculate it into the culture medium for culture; b. Access the above seed solution to the primary fermentation tank, culture for 3 hours, and pressurize the air tank to a 5000L fermentation tank; c. Access to the above 500L fermentation tank seed solution culture to the secondary fermentation tank fermentation, the protein mother liquor, spare; d. Premix 50-70 parts of seaweed mother liquid with 5-15 parts of organic matter for later use; e. Add 20-30 parts of protein mother solution (including protein stabilizer) to the above seaweed organic matter premix to obtain a finished product of seaweed combined water-soluble fertilizer containing plant immune activating protein.

4. The method for preparing a combined water-soluble fertilizer containing plant immune activating protein and seaweed according to claim 3, characterized in that: In step a, the culture medium components include tryptone, yeast extract powder, sodium chloride, and purified water, which are sterilized by wet heat at 150° C. for 50 min and cooled to 25° C. for standby use.

5. The method for preparing a combined water-soluble fertilizer containing plant immune activating protein and seaweed according to claim 3, characterized in that: The specific steps of the secondary fermentation in step c are: weigh the secondary fermentation raw materials and feed them into a 5000L tank, stir and dissolve, perform wet heat sterilization at 150°C for 50 minutes, and after cooling, add the 500L fermentation tank seed liquid to start culturing; adjust the pH with alkali solution, add a defoamer to control the foam, and add sugar. After the fermentation is completed, release the tank to obtain a protein mother liquor.

6. The method for preparing a combined water-soluble fertilizer containing plant immune activating protein and seaweed according to claim 3, characterized in that: The specific steps described in step e are: continue stirring for 3-4 hours after premixing, add acid to adjust the pH to 2-4, then put it in a tank for sedimentation for 48 hours, and filter it with a 50μm filter to obtain a combined water-soluble fertilizer product, in which the content of plant immune activating protein is 0.05-0.25g / L.

7. Applying the combined water-soluble fertilizer containing plant immune activating protein and seaweed as described in claims 1-6 to the growth of blueberries and tomatoes.

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