Immune colloidal gold test paper capable of simultaneously detecting canine distemper and canine parvo of foxes, raccoon dogs and minks and a method for preparing immune colloidal gold test paper

Abstract

The invention discloses immune colloidal gold test paper capable of simultaneously detecting canine distemper and canine parvo of foxes, raccoon dogs and minks and a method for preparing the immune colloidal gold test paper. The test paper consists of a test paper box and a test paper tape, and the test paper tape is made by sequentially bonding a hard polyvinyl chloride lining plate, a nitrocellulose membrane, a long colloidal gold combination pad, a short colloidal gold combination pad, a sample pad and an absorption pad. VP2 gene monoclonal antibody- colloidal gold biomarkers resistant to mink-source canine distemper viruses and parvoviruses are sprayed on the long and short colloidal gold combination pads respectively. Two detection lines formed by rabbit anti-mink source canine distemper virus and parvovirus antibodies and a quality control line formed by a rabbit antimouse antibody are sprayed on the nitrocellulose membrane. The test paper is applicable to quick detection of pestilences of foxes, raccoon dogs and minks, and detection of two pestilences including canine distemper and canine parvo of foxes, raccoon dogs and minks can be simultaneously completed by the same test paper, and detection is simple, convenient, quick and accurate.

Description

technical field [0001] The invention relates to a detection device for breeding animal diseases, in particular to an immune colloidal gold test paper capable of simultaneously detecting canine distemper and parvovirus in foxes, raccoon dogs and minks. The invention also relates to a preparation method of the test paper. Background technique [0002] Canine distemper (CD) is caused by canine distemper virus (CDV) and is a highly contagious disease of dogs and many animals in the order Carnivora, including foxes, raccoons, and minks; parvovirus disease (Canine Parvo, CP) is an acute infectious disease of dogs including foxes, raccoons and minks caused by parvovirus (Canine Parvovirus, CPV). These two infectious diseases often cause a large number of animals such as foxes, raccoons, and minks to become ill, and the fatality rate is generally between 30% and 80%. With the substantial increase in the breeding of fur animals such as foxes, raccoons, and minks in my country, and ...

AI Technical Summary

Problems solved by technology

If canine distemper or (and) parvovirus disease occurs clinically in foxes, raccoons and minks, only the colloidal gold test paper for detecting canine distemper or (and) parvovirus disease can be used to prepare canine distemper or ( and) Antigens of monoclonal antibodies against canine distemper virus or (and) parvovirus needed for colloidal gold test paper for parvovirus disease are all isolated from dogs, which may cause false positives or false negatives when used on foxes, raccoons, and minks As a result, poor accuracy

So far, there is no monoclonal antibody prepared from a pathogen isolated from mink as an antigen to make immunochromatographic colloidal gold test paper, and there is no test paper that can simultaneously detect canine distemper and parvovirus disease in foxes, raccoon dogs, and mink

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