Preparation method of spleen byproducts for producing homology anti-serum blood and transfer factor from fox, raccoon dog, mink

A transfer factor and serum technology, which is applied to medical preparations containing active ingredients, peptide sources, antibodies, etc., can solve the problems of less antiserum, high feeding cost, allergic reaction to serum heterologous components, etc. Breeding benefits and the effect of controlling the spread of the epidemic

Inactive Publication Date: 2009-05-06
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the antiserum for emergency use of fur animals has always been based on the antiserum of xenogeneic animals (such as dogs, sheep, donkeys, rabbits, etc.), and the antisera of xenogeneic animals are prone to allergic reactions to heterologous components in serum, and the xenogeneic animals used to prep...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Mink, choose healthy individuals, starting 45 days before the date of skin extraction, inject the animal with specific transfer factor once on the 0th day, inoculate the normal dose of attenuated vaccine once, and then inoculate again on the 10th day, and double the dose of vaccine or the same amount on the 24th day The source tissue inactivated vaccine was vaccinated once; the normal inoculation dose of canine distemper attenuated vaccine was 10 5 TCID 50 ; Mink enteritis virus inactivated vaccine once normal inoculation dose: 10 6 TCID 50 ; Preparation of homologous tissue inactivated vaccine: endemic wild strains cause animal disease, take the spleen and liver tissue of the diseased animal, fully freeze and thaw the tissue 4 times, and homogenate according to the volume of normal saline / tissue weight ratio of 10:1, Centrifuge at 2000g for 15 minutes, and take the supernatant to prepare formaldehyde-inactivated vaccine with aluminum hydroxide adjuvant; during the th...

Embodiment 2

[0013] Foxes, choose healthy individuals, starting 45 days before the date of skin extraction, inject the specific transfer factor once on the 0th day, inoculate the normal dose of the attenuated vaccine once, and inoculate again on the 10th day, and double the dose of the vaccine on the 24th day or use Inoculation of homologous tissue inactivated vaccine once; normal vaccination dose of canine distemper attenuated vaccine once: 10 5.47 TCID 50 , canine enteritis virus attenuated vaccine fox one normal inoculation dose: 10 6.47 TCID 50 , Adenovirus attenuated seedling fox one normal inoculation dose: 10 7 TCID 50 ; Preparation of homologous tissue inactivated vaccine: endemic wild strains cause animal disease, take the spleen and liver tissue of the diseased animal at a volume / weight ratio of 10:1, homogenate, fully freeze-thaw 4 times, centrifuge at 2000g for 15min, The supernatant was taken to prepare formaldehyde-inactivated vaccine with aluminum hydroxide adjuvant. Du...

Embodiment 3

[0015] Raccoons, choose healthy individuals, start 45 days before the date of skin extraction, on the 0th day, inject the specific transfer factor once and inoculate the normal dose of attenuated vaccine once, then inoculate again on the 10th day, and double the dose of vaccine or the same time on the 24th day The source tissue inactivated vaccine is inoculated once; the normal inoculation dose of canine distemper attenuated vaccine is 10 5.47 TCID 50 ; Canine enteritis virus attenuated vaccine raccoon dog once normal inoculation dose: 10 6.47 TCID 50 ; Adenovirus attenuated vaccine raccoon dog once normal inoculation dose 10 7 TCID 50 ; Preparation of homologous tissue inactivated vaccine: endemic wild strains cause animal disease, take the spleen and liver tissue of the diseased animal, fully freeze and thaw the tissue 4 times, and homogenate according to the volume of normal saline / tissue weight ratio of 10:1, Centrifuge at 2000 g for 15 min, and take the supernatant to...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a preparation method for obtaining blood used for preparing homologous antiserums and a spleen byproduct used for preparing transfer factors from the bodies of fur bearing animals such as foxes, raccoon dogs and minks; the furs of the foxes, the raccoon dogs and the minks are taken concentratedly in December and confirmed according to the fur-taking dates of different areas; healthy individuals are picked up 45 days before a predicated fur-taking date; the booster immunization of univalent vaccines or polyvalent vaccines of canine distemper, Canine parvovirus enteritis, adenovirus disease, corona virus laxness, parainfluenza, and pasteurellosis is carried out for 3 times by purified and condensed antigens on the foundation of carrying out immunization for once in summer; when the furs are taken, aseptic anticoagulation blood collection is carried out to a heart to improve the blood serum yield; and the spleens of the animals are collected for extracting specific or common transfer factors simultaneously. The blood can be conveniently prepared into the blood serums which resist communicable diseases, and the transfer factors can be extracted the spleen, thus providing guarantee for the healthy development of the breeding in the next year; the invention is used for curing the corresponding communicable diseases of the wild animals of the same species; and when a small amount of communicable disease cases appear in a breeding crowd, the corresponding pathogeny hyper-immune serums and transfer factors are mainly injected to a supposed healthy crowd after the pathogeny is confirmed so as to cure the affected animals in the early period of disease and control the spreading of the epidemic situations.

Description

Technical field: [0001] The present invention relates to a method for preparing homologous antiserum and transfer factor of foxes, raccoon dogs and minks, in particular to a method for preparing anti-infectious disease serum from fur animal foxes, raccoon dogs and minks simultaneously and using The preparation method of the spleen for the preparation of the transfer factor is used to prepare low-cost homologous antiserum and the raw material of the transfer factor. Background technique [0002] Fur animals such as foxes, raccoon dogs, and mink are prone to infectious diseases in high-density breeding, especially canine distemper, parvovirus enteritis, adenovirus disease, coronavirus diarrhea, parainfluenza, and pasteurellosis, which are highly contagious and have a high fatality rate. The animal breeding industry is seriously harmful; the antiserum produced by the body caused by viruses and germs is a special medicine for the early treatment of the corresponding disease; the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K35/14A61K35/28A61K35/16A61K38/17A61K39/00A61K39/395A61P31/04A61P31/14C07K14/47
Inventor 邵西群杨福合闫喜军柴秀丽王长凤章秀婷王凤雪
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products