Egg yolk antibody for preventing and controlling duck parvovirus and preparation method thereof

A technology of duck parvovirus and egg yolk antibody, which is applied in the field of egg yolk antibody for the prevention and treatment of duck parvovirus and its preparation, can solve the problems of economic loss in meat duck breeding industry, lower qualified rate of ducks slaughtered, and increased feed-to-meat ratio of duck flocks. The preparation method is easy to operate, has stable properties and strong specificity

Active Publication Date: 2015-12-16
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The disease mainly leads to an increase in the feed-to-meat ratio of duck flocks, an increase in breeding costs, and

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation of embodiment 1GT2015 inactivated vaccine

[0036] (1) Virus propagation procedure Inoculate 9-day-old healthy duck embryos with GT2015 strain at 0.2 mL / embryo, harvest virus allantoic fluid after 120 hours, and store at -20°C;

[0037] (2) Virus inactivation virus allantoic fluid filtration treatment, add formaldehyde solution to a final concentration of 0.1%, and then inactivate at 37°C for 16 hours, shake 3-4 times during the period, and store at 4°C;

[0038] (3) Preparation of inactivated vaccine

[0039] a. Preparation of oil phase: 94 parts of white oil, 806 parts of Span-806 parts, mix and heat, add 2 parts of aluminum stearate and mix evenly to obtain the oil phase;

[0040] b. Preparation of the water phase: 4 parts of Tween-80, 96 parts of inactivated and concentrated virus antigen solution, mixed evenly to obtain the water phase;

[0041] c. Mix and emulsify 6 parts of the oil phase into the colloid mill, start the machine to stir slowly, a...

Embodiment 2

[0042] The preparation of embodiment 2GT2015v live vaccine

[0043] Propagation of vaccine strains: Well-developed 9-day-old healthy duck embryos and attenuated GT2015v strain of duck parvovirus were selected for virus propagation, cultured at 37°C for 5 days, virus allantoic fluid was collected, and stored at -20°C.

[0044] Mixing seedlings and freeze-drying: mix the qualified duck parvovirus solution with 5% sucrose skim milk according to the ratio required for the feathers of the seedlings, divide and pack and carry out freeze-drying.

Embodiment 3

[0045] Example 3 Preparation of egg yolk antibody for preventing and treating duck parvovirus

[0046] 1. Select 90-day-old healthy laying hens, and use the duck parvovirus oil emulsion inactivated vaccine for the first immunization (subcutaneous injection of 1 mL in the neck); at intervals of 7 days, use the duck parvovirus oil emulsion inactivated vaccine for the second immunization ( Neck subcutaneous injection 2mL); interval 14 days, use duck parvovirus oil emulsion inactivated vaccine for the third immunization (neck subcutaneous injection 3mL); interval 21 days, use duck parvovirus oil emulsion inactivated vaccine for the fourth time Immunization (3 mL subcutaneously in the neck). After the fourth immunization, the agar diffusion test was used to detect that the serum antibody titer reached 1:64, the eggs laid were collected, and the yolk was aseptically separated.

[0047] 2. Dilution: under aseptic conditions, according to the ratio (volume ratio) of egg yolk and adde...

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PUM

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Abstract

The invention relates to the technical field of biological products, in particular to an egg yolk antibody for preventing and controlling a duck parvovirus and a preparation method thereof. The egg yolk antibody is derived from primiparity eggs of healthy laying hens which are processed through high-intensity combined immunization by using duck parvovirus live vaccines and duck parvovirus inactivated vaccines. According to the egg yolk antibody, the preparation method is easy to operate and suitable for mass production, and the prepared duck parvovirus egg yolk antibody is stable in property, high in purity, potency and specificity, free of drug residues, mild and environmentally friendly, extremely has the application value on prevention and treatment of duck parvovirus infection and supplies a new way to preparation of the duck parvovirus egg yolk antibody.

Description

technical field [0001] The invention relates to the field of biological products, and provides an egg yolk antibody for preventing and treating duck parvovirus and a preparation method thereof. Background technique [0002] Duck parvovirus is a kind of parvovirus, which mainly affects ducklings aged 1 to 3 weeks. The main clinical symptoms are diarrhea, dyspnea and soft feet. It occurs all year round and brings great economic benefits to duck breeding. Loss is one of the main diseases in duck breeding, and the morbidity and mortality are also high. (There are few introductions about duck parvovirus, please add the relevant introduction and hazards of this virus) [0003] Since March 2015, a disease has appeared in meat ducks in Shandong, Jiangsu, Anhui and other places in my country. The disease is characterized by dysplasia of duck beak and protruding tongue. The disease mainly occurs in commercial ducks over 14 days old. The incidence rate of duck flocks is 10%-20%, up to...

Claims

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Application Information

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IPC IPC(8): C07K16/08C07K16/02C12N7/00C12R1/93
Inventor 刁有祥陈浩郑肖强窦砚国牛晓宇杨晶于相龙
Owner SHANDONG AGRICULTURAL UNIVERSITY
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