Gosling plague antibody test standard substance and preparation method thereof

A test standard, gosling plague technology, applied in biological material analysis, measuring devices, instruments, etc., to achieve the effects of good specificity, high accuracy of determination, and wide application range

Inactive Publication Date: 2018-03-02
哈药集团生物疫苗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

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  • Gosling plague antibody test standard substance and preparation method thereof
  • Gosling plague antibody test standard substance and preparation method thereof
  • Gosling plague antibody test standard substance and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 The preparation and inspection of the amplified antigen of Goose Pyramid

[0037] 1. Preparation of poisonous seeds

[0038] Goose parvovirus H strain (preservation number: CGMCC No.6851) was diluted 1000 times with normal saline, and 12-day-old susceptible goose embryos were inoculated into the allantoic cavity, 0.2 ml per embryo. Incubate at 37°C, take out goose embryos that died within 72 to 168 hours, collect goose embryo liquid, put it in a sterile container, and at the same time absorb part of the goose embryo liquid for sterility testing and virus content determination, and pass the inspection of goose embryo liquid Store at -20°C.

[0039] 2. Preparation and packaging

[0040] Goose embryo liquid is added 5% sucrose skimmed milk according to ratio 1:1 (volume ratio) as protective agent (by g / ml, the final concentration of sucrose in the described lyoprotectant is 2.5%, the final concentration of skimmed milk is 2.5% %, the balance is water), quantit...

Embodiment 2

[0057] The optimization of embodiment 2 gosling plague immunization program

[0058] 1. Immunogen dosage optimization

[0059] 1.1 Test grouping

[0060] Sixty 40-day-old healthy susceptible geese were divided into 6 groups, 10 in each group. At the same time, another 10 healthy controls were set up and kept in isolation.

[0061] 1.2 Immunization

[0062] The gosling plague immunogen (preparation method is the same as in Example 3) was inoculated according to the immunization program in Table 1. The control group was injected with normal saline and kept in isolation.

[0063] Table 1 Gosling plague immunization program

[0064]

[0065] 1.3 Determination of antibody titer

[0066] After inoculation of healthy susceptible geese according to the above-mentioned 6 groups of immunization procedures, 2.0ml blood of healthy susceptible geese was collected at 4 time points before basic immunization, before booster immunization, before booster immunization and 14 days after ...

Embodiment 3

[0086] Example 3 Preparation and testing of gosling plague positive serum

[0087] 1. Immunogen preparation

[0088] 1.1 Inoculation

[0089] The goose parvovirus H strain production virus seed was diluted 1000 times with physiological saline, and the allantoic cavity was inoculated with 12-day-old susceptible goose embryos, 0.2ml per embryo. Incubate at 37°C without turning the eggs.

[0090] 1.2 Incubation and observation

[0091] After inoculation, goose embryos that died before 72 hours were discarded. After 72 hours, light the eggs once every 4 to 8 hours, and take out the goose embryos that die within 72 to 168 hours at any time, and cool the air chamber upright at 2 to 8°C.

[0092] 1.3 harvest

[0093] Take out the goose embryos that have been cooled for 6 to 12 hours, disinfect the air chamber with tincture of iodine, and then collect the goose embryo fluid (allantoic fluid and amniotic fluid) by aseptic operation. While harvesting, check the lesions of the embry...

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Abstract

The invention discloses a gosling plague antibody test standard substance and a preparation method thereof. The gosling plague antibody test standard substance includes a gosling plague agar diffusionantigen, a gosling plague positive serum and a gosling plague negative serum. Preparation of the gosling plague agar diffusion antigen includes the steps: inoculating a susceptible goose embryo witha goose parvovirus strain, and harvesting a goose embryo liquid, to obtain an antigen liquid; and adding a freeze-drying protective agent to the antigen liquid, and freeze-drying to obtain the goslingplague agar diffusion antigen. Preparation of the gosling plague positive serum includes the steps: inoculating a susceptible goose embryo with the goose parvovirus strain, and harvesting a goose embryo liquid, to obtain an antigen liquid; and concentrating and inactivating the antigen liquid to prepare a gosling plague immunogen, immunizing a healthy susceptible goose, sampling blood, separatingserum, and freeze-drying to obtain the gosling plague positive serum. Preparation of the gosling plague negative serum includes the steps: sampling an adult goose which is negative to a gosling plague antibody, separating serum, and freeze-drying to obtain the gosling plague negative serum. The gosling plague antibody test standard substance has the advantages of good specificity and high fixed value accuracy and has application prospects in prevention and control of gosling plague.

Description

technical field [0001] The invention relates to a gosling plague antibody test standard substance, and also relates to a preparation method of the gosling plague antibody test standard substance, belonging to the field of preparation of gosling plague antibody test standard substances. Background technique [0002] Gosling plague is an acute or subacute septic infectious disease of goslings caused by Goose Parvovirus (GPV). The disease was first discovered in Yangzhou, Jiangsu Province by Fang Dingyi, a Chinese scholar, in 1956. Since the 1960s, the occurrence and prevalence of the disease have been reported successively in countries such as Europe and Asia. In 1974, the International Poultry Disease Society (WPSA) officially named the disease Derzsys' disease. The disease mainly affects goslings aged 4 to 20 days after hatching, and also infects ducklings. It spreads quickly, and the morbidity and mortality are as high as 90% to 100%. There is an epidemic, which causes se...

Claims

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Application Information

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IPC IPC(8): G01N33/569
CPCG01N33/56983G01N2333/183G01N2333/465
Inventor 藏玉婷柴华梁宛楠赵辉张春媛李鑫邢育钢宋扬刘鑫莹郭照成陈欣孙晓峰李建华
Owner 哈药集团生物疫苗有限公司
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