30 results about "Plague vaccine" patented technology

This disclosure concerns compositions and methods for the treatment and inhibition of infectious disease, particularly bubonic and pneumonic plague. In certain embodiments, the disclosure concerns immunogenic proteins, for instance substantially monodisperse F1-V fusion proteins, that are useful for inducing protective immunity against Y. pestis.

Techniques from two basic approaches, structure-based immunogen design and phage T4 nanoparticle delivery, are developed to construct new plague vaccines. The NH₂-terminal β-strand of F1 of Yersinia pestis is transplanted to the COOH-terminus of F1 of Yersinia pestis and the NH₂-terminus sequence flanking the β-strand of F1 of Yersinia pestis is duplicated to eliminate polymerization but to retain the T cell epitopes. The mutated F1 is fused to the V antigen of Yersinia pestis to thereby form a fusion protein F1mut-V mutant, which produces a completely soluble monomer. The fusion protein F1mut-V is then arrayed on phage T4 nanoparticles via a small outer capsid protein, Soc, from a T4 phage or a T4-related phage. Both the soluble and T4 decorated F1mut-V provided approximately 100% protection to mice and rats against pneumonic plague evoked by high doses of Yersinia pestis CO92.

The invention discloses a protective antigen composition which can motivate an organism to generate immunity to plague, and the application thereof in preparing a plague vaccine. The protective antigen composition is a composition which is mixed by a natural F1 antigen and an rV270 antigen according to the weight ration of 1-2 to 2-1. The immune protection effect of the composition is evaluated by animal experiments and the evaluation result shows that the protective antigen composition that is composed of the natural F1 antigen and the rV270 antigen performs good immune protection effect to the infection caused to mice, guinea pigs and rabbits by plague bacillus, has relatively high safety and can be used for preparing a subunit plague vaccine.

Provided is an ecological breeding method for beef cattle. The method includes the following steps of selecting a small hill with good air, shade and less wind; arranging ventilation and heating facilities in a cattle barn, and arranging a shower area; injecting plague vaccine for 30 - 40 days of beef cattle pups, and injecting combined vaccine against the plague and erysipelas lung diseases for 60 - 70 days of beef cattle pups; in the beef cattle fattening stage, stocking in the morning, breeding in the barn in the afternoon, putting cattle into the shower area to conduct shower cleaning every 3-5 days, keeping ventilation in the cattle barn, maintaining 22-26 DEG C of temperature at daytime, and maintaining 14-21 DEG C of temperature at night. The ecological breeding method for beef cattle is scientific and reasonable, combines the advantages of stocking and barn breeding, starts from the growth characteristics of the cattle, provides the cattle with reasonable nutrients, ensures proper movement of the cattle, and thus enhances the physique of beef cattle. The bred beef cattle are strong, the quality of the beef is guaranteed well, and the produced beef has the advantages of being rich in nutrient, good in taste and high in nutritive value, and meets people's demands for beef nutrition better.

The invention discloses a plague bacteria protein with cellular immune protection function, an encoding gene and an application. The purpose is to provide the protein and the encoding gene which can stimulate T cell immune reaction during plague bacteria infection and immune processes and can generate cellular immune protection function, as well as the application in the preparation of plague vaccines. The experiments prove that the protein of the invention can stimulate T cell immune reaction during the infection and the immune processes of plague bacteria and can generate immune protection function. The protein or gene of the invention can be taken as an active ingredient for preparing the plague vaccines, and can enhance the immune protection effect and the immune protection region of the existing plague vaccines through mutual immunization or the combined immunization with the existing F1 and LcrV antigen or gene with immune protection function, or the preparation of a recombinant fusion multivalent subunit vaccine or DNA vaccine at the same time. The invention provides a new means for searching the protein of the plague bacteria with cellular immune protection function, which can play the important role in the prevention and the treatment of plague and have broad application prospect.

The invention provides a weaned young rabbit breeding method. The weaned young rabbit breeding method comprises the following steps: adding 5% milk powder into feed for a weaned young rabbit segmentally; adding a rabbit vibrio eliminating medicine into water or the feed to prevent enteritis of the young rabbit; injecting rabbit plague vaccine to prevent rabbit plague of the young rabbit; and adding sulfamide coccidian and diclazuril into the feed or water to prevent and control coccidiosis of the young rabbit. According to the weaned young rabbit breeding method, through comprehensive utilization of determination on a young rabbit weaning standard, feed control and management, and segmental prevention of the enteritis, the rabbit plague and the coccidiosis, the normal growth and development of the weaned young rabbit can be ensured, the survival rate of the young rabbits is increased from 65% to 95%, the young rabbits grow rapidly and can be slaughtered earlier, and the meat quality and the fur quality are high; therefore, the weaned young rabbit breeding method is applicable to breeding rabbits.

This invention provides one rapid test bar for plague vaccine, which covers plague vaccine F1 antigen and plague antibiosis F1 multiple clones antigens on the NC film and combines glue gold label antigen and applies film analysis double antigen clamper method and tests human and animal blood specimen plague antibiosis level.

A method of protecting a human or animal body from the effects of infection with Y. pestis is provided comprising administering to the body a vaccine including Yersinia pestis V antigen and Yersinia pestis F1 antigens or a protective epitopic part of each of these in a form other than whole Y. Pestis organisms. Preferably the antigens are administered in the form of a live vaccine or as recombinantly produced isolated and/or purified proteins. DNA encoding the whole or part of the F1 antigen and DNA encoding the whole or part of the V antigen may be used directly as a genetic vaccine.

This invention relates to vaccine formulations comprising the Yersinia pestis YopE peptide antigen or subparts thereof. The invention also relates to methods of vaccinating subjects at risk of Yersinia pestis infection as well as assays for measuring immune response to plague vaccines.

Disclosed herein is a composition comprising a purified fusion protein comprising all or part of F1 antigen of Yersinia pestis fused to the amino terminus of all of V antigen of Yersinia pestis, Yersinia enterocolitica, or Yersinia pseudotuberculosis that is isolated from its expression vector.

The invention provides a composite plague vaccine and the preparation method thereof. The plague vaccine comprises thallus split products of the attenuated strain of plague bacilli and recombination plague bacilli V antigens, and further comprises plague bacilli F1 antigens, wherein, by collecting the thallus of plague attenuated strain grown on the culture medium, the thallus split products of the plague attenuated strain is obtained through the cracking preparation by high-pressure homogenization. Bacterial cell wall with good adjuvant effect, bacterial protein with antigenic specificity (which includes protective capsular antigen F1) and CpG fragments which are rich in bacterial DNA, with stronger immunological competence, are concentrated in the split products; the expression vector including plague bacilli V antigens or F1 antigens which are constructed by adopting the gene recombination technology is transformed into escherichia coli, and after the inducible expression is performed, recombination plague bacilli V antigens or F1 antigens are obtained through purification. The vaccine is applicable to the general population for immunizing and preventing the plague.

The invention aims at providing a muscovy-duck-source gosling plague virus inactivated vaccine. The vaccine comprises an antigen and a vaccine adjuvant. The adopted antigen is an inactivated muscovy-duck-source gosling plague virus, and the preservation number of the adopted muscovy-duck-source gosling plague virus is CCTCC NO:V201620. A muscovy-duck-source gosling plague virus YBGPV-M strain with low toxicity and high immunogenicity is screened out and then inoculated with a duck embryo, the infected embryo and allantoic fluid are collected, after homogenization, ultrafiltration and concentration and formaldehyde solution inactivation, the oil adjuvant is added, and the vaccine is prepared after mixing and emulsifying. The prepared vaccine immunizes breeding muscovy ducks, the antibody level of the breeding muscovy ducks can be improved, the offspring maternal antibody level of the breeding muscovy ducks is guaranteed, and young muscovy duck gosling plague virus infection caused by the muscovy-duck-source gosling plague virus is prevented; antibodies can be produced after 10 days when 1-day young muscovy ducks are immunized, and young muscovy duck gosling plague virus infection caused by the muscovy-duck-source gosling plague virus can be effectively prevented. The prepared vaccine has the advantages of being efficient and good in safety.

The invention discloses a method for removing lipoprotein and lipid in goose/duck yolk by using a freezing method. The method comprises the following steps of (1) cleaning and disinfecting a fresh egg laid by a goose/duck supplementarily immunized by using a goose vaccine; (2) separating egg white from yolk; (3) extracting 1 part of separated yolk according to a certain volume, adding 3 times of distilled water with the same volume to dilute, uniformly stirring, and storing under freezing at the temperature of 18 DEG C below zero for 24h and longer to ensure that the yolk is solidly frozen; (4) taking out the solidly frozen yolk, unfreezing, and filtering to obtain colorless and transparent filtrate capable of purifying immunoglobulins IgY. The goose vaccine in the step (1) is a gosling plague vaccine, a goose paramyxovirus vaccine or an avian influenza vaccine. By using a physical freezing separation method, a yolk sediment is sufficiently utilized, and no chemical organic solvents are adopted, so that the cost can be remarkably reduced.

The invention discloses a breeding method for black swans in the southern region of China. The method comprises the following steps: a stocking water area is provided for the black swans according toan area of 65-75m<2> of each pair of black swans, and at least one place with trees or shrubs exist near the water area; the water area is disinfected once every three months; in the gosling period, feeding is performed for three times a day by using first concentrated feed, feeding is performed once a day by using green feed, and water is sufficient, in the young goose and adult goose periods, feeding is performed twice a day by using first type feed, and in the egg producing stage of breeding geese, feeding is performed four times a day by using second concentrated feed, and green feed is freely supplied; a gosling plague vaccine is injected within one week to half a month after goslings are born, and a goose paramyxovirus vaccine is injected from March 15 to April 15 every year; and enough nesting materials are provided near the water area, after the black swans build a nest freely, a shack is built at the top of the nest, and cleanliness and hygiene near the shack are guaranteed. According to the method provided by the invention, in addition to partial artificial intervention in the gosling period, the bred black swans are mainly free-range, and the bred black swans have a black and bright hair color, a healthy body posture, less illness and a greater ornamental value.

Bivalent immunogenic compositions against anthrax and plague are disclosed herein. One bivalent immunogenic composition comprises a triple fusion protein containing three antigens, F1 and V from Yersinia pestis and PA antigen from Bacillus anthracia fused in-frame and retaining structural and functional integrity of all three antigens. Another bivalent immunogenic composition comprises bacteriophage nanoparticles arrayed with these three antigens on the capsid surface of the bacteriophage nanoparticles. These bivalent immunogenic compositions are able to elicit robust immune response in a subject administered said the bivalent immunogenic compositions and provide protection to the subject against sequential or simultaneous challenge with both anthrax and plague pathogens.

The application relates to a Yersinia pseudotuberculosis cell, which comprises nucleic acid coding for expression of at least one Yersinia pestis Caf1 polypeptide or of at least one antigenic fragment of Yersinia pestis Caf1, more particularly to an attenuated Y. pseudotuberculosis cell, which expresses the Y. pestis capsule. Said Y. pseudotuberculosis cell is exceptionally efficient in protecting against both bubonic plague and pulmonary plague.