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Test paper bar for testing colloidal gold of F1 antibody of plague bacterium

A technology for detecting test paper and test strips, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of long time, complicated operation, cumbersome and other problems, and achieve the effect of easy promotion, clear and easy to distinguish results, and simple operation

Inactive Publication Date: 2007-05-16
BEIJING ZHUANGDI HAOHE BIOMEDICINE SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a convenient and fast test strip for detecting the Yersinia pestis F1 antibody, so as to overcome the shortcomings of the prior art such as complex operation, cumbersome operation, long time, need for instruments, equipment, and refrigeration of specimens

Method used

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  • Test paper bar for testing colloidal gold of F1 antibody of plague bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Test strips for detection of Yersinia pestis F1 antibody (see Figure 1)

[0029] The reaction support is 6.5cm×0.4cm PCV plate; the absorbent pad is 2cm×0.4cm oil filter paper; 1.8cm×0.4cm nitrocellulose membrane is coated with polyclonal antibody against Yersinia pestis F1 antigen and Yersinia pestis F1 antigen in turn; A glass fiber membrane containing 0.4cm×0.4cm colloidal gold-labeled Yersinia pestis F1 antigen; the gold-labeled antigen protective film is a 2.7cm×0.4cm polyester membrane; that is, a test strip for the detection of Yersinia pestis F1 antibody is formed.

Embodiment 2

[0030] Example 2 Test strips for detection of Yersinia pestis F1 antibody (see Figure 1)

[0031] The reaction support is 6.5cm×0.4cm PCV plate; the absorbent pad is 2cm×0.4cm oil filter paper; 1.8cm×0.4cm nitrocellulose membrane is coated with polyclonal antibody against Yersinia pestis F1 antigen and Yersinia pestis F1 antigen in turn; A glass fiber membrane containing 0.4cm×0.4cm colloidal gold-labeled Yersinia pestis F1 antigen; a gold-labeled antigen protective film is a 2.7cm×0.4cm glass fiber; that is, a test strip for the detection of Yersinia pestis F1 antibody is formed.

Embodiment 3

[0032] Example 3 Test strips for detection of Yersinia pestis F1 antibody (see Figure 1)

[0033] The reaction support is 6.5cm×0.4cm PCV plate; the absorbent pad is 2cm×0.4cm oil filter paper; 1.8cm×0.4cm nitrocellulose membrane is coated with polyclonal antibody against Yersinia pestis F1 antigen and Yersinia pestis F1 antigen in turn; A glass fiber membrane containing 0.4cm×0.4cm colloidal gold-labeled Yersinia pestis F1 antigen; the gold-labeled antigen protective film is a 2.7cm×0.4cm filter paper fiber; that is, a test strip for the detection of Yersinia pestis F1 antibody is formed.

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Abstract

This invention provides one rapid test bar for plague vaccine, which covers plague vaccine F1 antigen and plague antibiosis F1 multiple clones antigens on the NC film and combines glue gold label antigen and applies film analysis double antigen clamper method and tests human and animal blood specimen plague antibiosis level.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a test strip for detecting Yersinia pestis F1 antibody and an application thereof. Background technique [0002] For the detection of Yersinia pestis F1 antibody, the commonly used methods are indirect hemagglutination (PHA) and enzyme-linked immunosorbent assay (ELISA). However, the above methods are complicated to operate, take a long time, require instruments and equipment, and require professionals to carry out in qualified laboratories. They are not suitable for grassroots units and remote areas, and are not suitable for the needs of traffic control and anti-terrorism. Contents of the invention [0003] (1) Technical problems to be solved [0004] The purpose of the present invention is to provide a convenient and quick test strip for detecting Yersinia pestis F1 antibody, so as to overcome the shortcomings of the prior art such as complex operation, cumber...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/544
Inventor 刘明
Owner BEIJING ZHUANGDI HAOHE BIOMEDICINE SCI & TECH
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