32 results about "Indirect hemagglutination" patented technology

The invention relates to a preparation method of a recombinant protein, an indirect classical swine fever hemagglutination test kit that is prepared by the protein, and a preparation method of the test kit. The preparation method of the protein comprises following steps: a segment with the gene homeology degree comparing with a bovine viral diarrhea-mucosal disease virus (BVDV) that is lower than35 percent is selected from a conservative region of a classical swine fever virus (CSFV) gene, thus obtaining a nucleotide sequence S1 that is corresponding to an amino acid A1; a forth amino acid in the nucleotide sequence S1 is modified to an hydrophilic amino acid, thus obtaining an amino acid A2; a corresponding nucleotide sequence S2 of A2 is modified, thus respectively obtaining three new nucleotide sequences; the three new nucleotide sequences are orderly and serially connected by connecting nucleotides, thus obtaining a sequence S9 that is corresponding to an amino acid sequence A3; and a nucleotide sequence S10 is obtained by processing S9 correspondingly, and after artificially synthesizing and digesting S10, connecting S10 with a carrier pGEX-4T-1 and inducing the recombinant carrier into colon bacillus for induction expression, the recombinant protein is obtained by separating and purifying an expression product.

The invention provides an antigen for detecting a porcine infectious pleuropneumonia antibody of swinery and a preparation method of the antigen. The antigen is a polysaccharide antigen which is extracted from type 1-15 strain cultures of porcine infectious pleuropneumonia actinobacillus; the polysaccharide antigen is a bacterial excreted polysaccharide which has very strong specificity and sensibility and is capable of truly detecting the porcine infectious pleuropneumonia antibody; the determination of a normal concentration critical value of the antigen provides guarantee for the detection of enzyme linked immunosorbent assay and the detection of indirect hemagglutination test; the preparation method of the antigen is simple to operate, convenient and quick, and suitable for popularization and application at the basic level, and does not need any special equipment and instrument.

The invention discloses a pig mycoplasma pneumonia recombination antigen ELISA detection Kit. The Kit is provided with an antibody detection plate, enzyme conjugate treatment fluid, a positive control, a negative control, sample diluent, 10x condensed cleaning solution, developing solution A, developing solution B and termination solution. The detection plate of the Kit is a detachable 96-pore enzyme label plate enveloped by the mutational pig mycoplasma pneumonia membrane protein P46 gene protein antigen, the enzyme conjugate treatment fluid is a rabbit anti-pig antibody labeled by horse radish peroxidase, the positive control serum is taken from a pig which is detected positive through indirect hemagglutination and the ELISA Kit of IDEXX and has obvious pig mycoplasma pneumonia lesions in the lungs after anatomy, and the negative control serum is taken from a pig which is detected negative through indirect hemagglutination and the ELISA Kit of IDEXX and has no pig mycoplasma pneumonia lesions in the lungs after anatomy. The pig mycoplasma pneumonia recombination antigen ELISA detection Kit has the advantages of strong specificity, high sensitivity, simple operation, easy large-scale generation and application, and broad market prospect.

A mycoplasma capricolum subsp. pneumonia polysaccharide antigen of goats is characterized by extracting polysaccharides secreted by thalli from the supernatant of a mycoplasma capricolum subsp. pneumonia culture of goats. The agent is prepared by the following processing steps: a. culturing mycoplasma and b. extracting mycoplasma polysaccharide antigen. The invention has the following advantages:(1) the antigen in the invention is the polysaccharide antigen extracted from the supernatant of the mycoplasma capricolum subsp. pneumonia culture of goats and is polysaccharide secreted by thalli, has strong specificity and can truly detect the mycoplasma capricolum subsp. pneumonia antibody of goats; (2) the aldehyde-tanned sheep red cells sensitized with polysaccharide are used, thus avoidingthe defects of short preservation time and low sensitization titer of the fresh sheep red cells, being easily preserved for long time and having high hemagglutination titer;(3) the method is simple, convenient and fast in operation and dispenses with special equipment and apparatuses, thus being suitable for popularization and application at the grassroots level; (4) the invention is the unique diagnostic reagent for detecting the mycoplasma capricolum pneumonia antibodies of goats at home at present.

The invention provides a kit for detecting indirect hemagglutination of streptococcus suis. The kit comprises indirect hemagglutination antigen of streptococcus suis, and the indirect hemagglutination antigen of streptococcus suis is composed of whole bacterial protein of streptococcus suis CCTCC M 2016028 and aldehydated-tanned sheep red blood cell. The kit has the advantages that the operation is simple, convenient and rapid, no special equipment and apparatus is required, a whole course can be completed in 2-3 hours, the kit is suitable for basic popularization and application, and can be widely used for investigation and research of epidemiology of swine streptococcosis. The results have the advantages of good repeatability, stabilization, and reliablility, and can accurately detect the antibody of streptococcus suis.

The invention provides a detection kit for enterococcus faecium indirect blood clotting. The detection kit is characterized by comprising an enterococcus faecium indirect blood clotting antigen, wherein the enterococcus faecium indirect blood clotting antigen is composed of enterococcus faecium CCTCC M 2016047 whole bacterial proteins and hydroformylation-tannin sheep red blood cells. The kit provided by the invention is simple, convenient and quick in operation; special equipment and instrument are not required; the whole process can be completed within 2-3 hours; the detection kit is fit for popularization and application in basic level; the detection kit can be widely applied to the investigation of enterococcus faecium epidemiology; the repeatability of the result is excellent; the result is stable and reliable; the antibody of the enterococcus faecium can be accurately detected.

The invention provides a staphylococcus Warner indirect blood coagulation detection kit and application thereof. The kit is characterized by comprising a staphylococcus Warner indirect blood coagulation antigen, wherein the staphylococcus Warner indirect blood coagulation antigen is composed of whole bacterial protein of staphylococcus Warner CCTCC M2016048 and hydroformylated-tanned sheep red blood cells. The kit provided by the invention is simple to operate and is convenient and rapid, and special equipment and instruments are not needed; and a whole process is finished within 2-3 hours, so that the kit is suitable for being popularized and applied to a basic level and can be widely applied to investigation and study of staphylococcus Warner disease epidemiology. A detection result has good stability and is stable and reliable, and an antibody of staphylococcus Warner can be accurately detected.