31 results about "Indirect hemagglutination" patented technology

The invention discloses a pig mycoplasma pneumonia recombination antigen ELISA detection Kit. The Kit is provided with an antibody detection plate, enzyme conjugate treatment fluid, a positive control, a negative control, sample diluent, 10x condensed cleaning solution, developing solution A, developing solution B and termination solution. The detection plate of the Kit is a detachable 96-pore enzyme label plate enveloped by the mutational pig mycoplasma pneumonia membrane protein P46 gene protein antigen, the enzyme conjugate treatment fluid is a rabbit anti-pig antibody labeled by horse radish peroxidase, the positive control serum is taken from a pig which is detected positive through indirect hemagglutination and the ELISA Kit of IDEXX and has obvious pig mycoplasma pneumonia lesions in the lungs after anatomy, and the negative control serum is taken from a pig which is detected negative through indirect hemagglutination and the ELISA Kit of IDEXX and has no pig mycoplasma pneumonia lesions in the lungs after anatomy. The pig mycoplasma pneumonia recombination antigen ELISA detection Kit has the advantages of strong specificity, high sensitivity, simple operation, easy large-scale generation and application, and broad market prospect.

The invention relates to a preparation method of a recombinant protein, an indirect classical swine fever hemagglutination test kit that is prepared by the protein, and a preparation method of the test kit. The preparation method of the protein comprises following steps: a segment with the gene homeology degree comparing with a bovine viral diarrhea-mucosal disease virus (BVDV) that is lower than 35 percent is selected from a conservative region of a classical swine fever virus (CSFV) gene, thus obtaining a nucleotide sequence S1 that is corresponding to an amino acid A1; a forth amino acid in the nucleotide sequence S1 is modified to an hydrophilic amino acid, thus obtaining an amino acid A2; a corresponding nucleotide sequence S2 of A2 is modified, thus respectively obtaining three new nucleotide sequences; the three new nucleotide sequences are orderly and serially connected by connecting nucleotides, thus obtaining a sequence S9 that is corresponding to an amino acid sequence A3; and a nucleotide sequence S10 is obtained by processing S9 correspondingly, and after artificially synthesizing and digesting S10, connecting S10 with a carrier pGEX-4T-1 and inducing the recombinant carrier into colon bacillus for induction expression, the recombinant protein is obtained by separating and purifying an expression product.

The invention discloses a preparation method of sensitized chicken erythrocyte as well as an IBV (Infectious Bronchitis Virus) detection kit. The preparation method of the sensitized chicken erythrocyte comprises the following steps of cell curing, wherein fresh chicken erythrocyte is washed by using buffer liquor, diluted by adding aldehyde liquor, oscillated for reaction and washed to obtain cured chicken erythrocyte; cell tanning, wherein the cured chicken erythrocyte is diluted, and added with tannic acid to obtain tanned chick erythrocyte; and cell sensitization, wherein the tanned chicken erythrocyte is diluted, added with IBV liquor for mixing and acting to obtain the sensitized chicken erythrocyte. The sensitized chicken erythrocyte disclosed by the invention has very high specificity, and a detection result has very good conformance. An IHA (Indirect Hemagglutination Assay) agent prepared and obtained by the sensitized chicken erythrocyte has low cost and is easy to operate, so that the result can be observed within 25 minutes and detection time is greatly shortened.

The invention provides a kit for detecting indirect hemagglutination of streptococcus suis. The kit comprises indirect hemagglutination antigen of streptococcus suis, and the indirect hemagglutination antigen of streptococcus suis is composed of whole bacterial protein of streptococcus suis CCTCC M 2016028 and aldehydated-tanned sheep red blood cell. The kit has the advantages that the operation is simple, convenient and rapid, no special equipment and apparatus is required, a whole course can be completed in 2-3 hours, the kit is suitable for basic popularization and application, and can be widely used for investigation and research of epidemiology of swine streptococcosis. The results have the advantages of good repeatability, stabilization, and reliablility, and can accurately detect the antibody of streptococcus suis.

The invention discloses a polymerase chain reaction (PCR) detection method of porcine eperythrozoon, and the method successively comprises the steps of pathogen separation and purification, porcine eperythrozoon DNA extraction, primer design and synthesis, ordinary PCR detection, positive plasmid standard substance preparation and real-time fluorescent quantitative PCR detection. Compared with IHA (indirect hemagglutination assay), ELISA (enzyme-linked immuno sorbent assay) and other detection methods, the method has the advantages of high specificity, high sensitivity, high accuracy and reliability, can fast, simply, reliably and accurately detect eperythrozoon in a sample, and can also be used for epidemiological investigation and curative effect detection of eperythrozoonosis.

The invention discloses a method for indirect immunofluorescence assay (IFA) detection of eperythrozoonosis by use of a monoclonal antibody. The method includes establishment of a standard positive control and a standard negative control, antigen coating, fluorescence second antibody dilution, IFA determination and other steps. An antibody used in the method is a monoclonal antibody against porcine eperythrozoon, and compared with general antibodies used in IHA (indirect hemagglutination assay), ELISA (enzyme-linked immuno sorbent assay) and other detection methods, the antibody used in the method has the advantages of high specificity, high sensitivity, high accuracy and reliability, can fast, simply, reliably and accurately detect eperythrozoon in a sample, and can also be used for epidemiological investigation and curative effect detection of the eperythrozoonosis.

The invention relates to an establishing method for pasteurella multocida indirect haemagglutination assay and application of the pasteurella multocida indirect haemagglutination assay in pasteurella multocida vaccine potency testing. According to the method, the stable pasteurella multocida indirect haemagglutination assay with good sensitivity and repeatability is established on the basis of polysaccharide content in the capsular antigen of pasteurella multocida through screening the optimal dilution of antigens in indirect hemagglutination test; the assay is used to detect the indirect hemagglutination titer of the serum of vaccinated animals and explore a parallel relationship between the indirect hemagglutination titer of the serum and a virulent virus attack protection rate after immunization and is applied to potency testing of pasteurella multocida vaccines.

The invention discloses a detection method and application of a mycoplasma hyopneumoniae antibody. The mycoplasma hyopneumoniae antibody is detected by mixing P36 and P46 proteins expressed by escherichia coli with sensitized red blood cells. According to the method, mycoplasma hyopneumoniae specific proteins P36 and P46 are specifically selected, the indirect hemagglutination sensitization antigen prepared through escherichia coli expression and Ni column purification reacts with a mycoplasma hyopneumoniae antibody to the maximum extent, the reaction range and sensitivity are greatly increased, cross reaction with other mycoplasmas is avoided, escherichia coli expresses the sensitization protein antigen, the growth period is greatly shortened, and production cost is reduced. The method is advantaged in that the detection reagent is good in stability, high in sensitivity and good in repeatability, has no obvious cross reaction on other pathogenic antibodies, is simple, convenient and rapid to operate, and can be used for clinical sample detection, immune level monitoring and epidemiological investigation.

The invention discloses a total design scheme of an automatic workstation for a large scale of hemagglutination inhibition (HI) experiments, and application of the design scheme in development of hemagglutination inhibition experiment special instruments and detection of the hemagglutination inhibition experiments. The workstation is a special instrument designed for specific steps of the HI experiments, and no similar products exist before. Compared with a PCR and an ELISA workstation, the workstation can meet the specific requirements of the HI experiments and can conduct HI detection on a large scale; compared with ordinary manual operation HI experiments, detection efficiency can be improved, labor cost can be saved, and working strength can be reduced. The workstation can detect the HI antibody titer of human and animal avian influenza, Newcastle disease, adenovirus and the like, can also be used for detecting the indirect hemagglutination (IHA) antibody titer of the foot-and-mouth disease, swine fever and the like and has good application prospects in all stages of laboratories.

The invention discloses a colloidal gold immunochromatographic test paper for detecting toxoplasma gondii infection in cats, and provides the same and a preparation method for serum detection of toxoplasma gondii infection in cats. The prepared colloidal gold test paper for toxoplasma gondii infection in cats has simple, Fast, no special equipment and other characteristics. The test results are easy to interpret and are suitable for rapid clinical diagnosis and large-scale epidemiological investigation. The indirect colloidal gold detection method is used, wherein the purified recombinant protein SAG3 ensures the specificity of the present invention. Use Staphylococcus aureus (SPA) for labeling, which is readily available and inexpensive. Compared with the traditional indirect hemagglutination test and indirect immunofluorescence test, the detection speed is faster, avoiding contact with live insects, and ensuring the safety of testing personnel.

The invention discloses a goat pox indirect hemagglutination diagnosis reagent as well as a preparation method and a detecting method thereof. The hemagglutination diagnosis reagent comprises the following components by weight percent: 0.6-1.2% of goat pox virus antigen sensitized red blood cells, and 20-200 mu g/ml of purified goat pox virus antigen. Compared with the commonly used agar diffusion experiment, the goat pox indirect hemagglutination diagnosis reagent is prepared from purified goat pox virus antigen sensitized hydroformylated sheep red blood cells in the invention, thus, the goat pox virus antibody can be detected fast, accurately and simply, the result can be determined in 50 minutes, and the antibody with low valence can be detected; moreover, the goat pox indirect hemagglutination diagnosis reagent has good specificity, sensitivity and repeatability, and can be widely used for fast and substantive detection of goat pox virus antibodies in clinical vet, and can provide the basis for prevention, diagnosis and prevention and control of a disease.

The invention relates to a detection method of actinobacillus pleuropneumoniae in pig serums. The detection method comprises the steps as follows: randomly selecting serum samples of piglets and finishing pigs in a scale farm, firstly carrying out IHA (indirect hemagglutination test) method detection on the collected serum samples, and secondly carrying out APPELISA detection on the collected serum samples to detect the actinobacillus pleuropneumoniae in the pig serums. According to the detection method, the APP indirect hemagglutination test and the APPELISA test are jointly adopted, so that the detection result of the actinobacillus pleuropneumoniae is accurate, reliable, quick, flexible and stable without error, and the detection method is low in cost, simple in method, suitable for basic level, and brings great convenience for cultivation, disease prevention and disease treatment of pigs.

The invention discloses an indirect coagulation kit for detecting a serum antibody against Staphylococcus saprophyticus, and methods for preparing and using the kit. The kit comprises (1) the indirecthemagglutination antigen of Staphylococcus saprophyticus, (2) standard positive serum, (3) standard negative serum, and (4) a diluent. The invention also provides methods for preparing and using thekit. The invention provides the detection kit for the indirect coagulation antibody against Staphylococcus saprophyticus and application thereof. The kit is simple and convenient to operate, and is extensively applicable to various veterinary-related units and farms; the kit in the invention only needs simple instrument consumables during detection of samples, and the whole detection process can be completed in 2-3 hours; results are intuitive and reproducible; and the kit can realize fast, stable and accurate detection of antibodies against Staphylococcus saprophyticus.

The invention discloses an establishment method of a mycoplasma ovipneumoniae indirect hemagglutination detection method. According to the method, recombinant protein obtained by cloning and expressing a C terminal of a mycoplasma ovipneumoniae adhesin gene P113 is used as an antigen, healthy sheep erythrocytes are sensitized by the recombinant protein antigen, the optimal sensitization concentration and the optimal reaction conditions are screened for IHA titers of the same batch of serum, and the mycoplasma ovipneumoniae indirect hemagglutination detection method which is high in specificity, sensitivity and coincidence rate and easy and convenient to operate is established. The detection method can be applied to rapid detection of mycoplasma ovipneumoniae in the veterinary field, and provides a powerful tool for disease prevention, diagnosis, prevention and control.

The invention discloses a goat pox positive indirect hemagglutination diagnostic reagent capable of detecting goat pox antibody and a preparation method and detection method thereof. The preparation method comprises the following steps: preparing red blood cell suspension by mixing glutaraldehyde and sheep red blood cells; mixing the red blood cell suspension with equivalent purified goat poxvirus, and stirring, centrifuging and washing; and preparing 1% sensitized red blood cells from PBS containing 1% of newly born calf serum to obtain the goat pox positive indirect hemagglutination diagnostic reagent. The optimized preparation conditions are that: the red blood cells are fixed by a glutaraldehyde single-hydroformylation method so that the concentration of sensitized red blood cells is 0.8-1.0%, and the antigen content is controlled between 20 ug.mL<-1> and 200 ug.mL<-1> so as to achieve relatively good sensitization effect; and the detection method is performed according to a conventional positive indirect hemagglutination test method, the reaction temperature is 20-25 DEG C, and the reaction time is 50 minutes. The method has relatively good specificity, sensitivity and repeatability, and can be applied to veterinarian clinical rapid detection of goat pox antibody.

The invention disclose an indirect hemagglutination kit for detecting enterococcus hirae serum antibodies and a preparation method for preparing and using the kit. The kit comprises (1), enterococcushirae indirect hemagglutination antigens; (2), standard positive serums; (3), standard negative serums; (4), a diluting solution. The invention further provides a preparation and application method ofthe kit. The invention provides the enterococcus hirae indirect hemagglutination antibody detection kit and the application therefore. The kit has the advantages that the operation of the kit is simple and convenient, and the kit is extensively popularized and used in each base-level veterinarian related units and farms; the kit only needs simple instrument and consumable materials when a sampleis detected, the whole process can be completed within 2-3 hours, the result judgement is visualized, the repeatability is good, and the enterococcus hirae antibodies can be rapidly, stably and accurately detected.