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Toxoplasma indirect hemagg lutination diagnostic reagent and producing process thereof

A diagnostic reagent, the technology of Toxoplasma gondii, which is applied in the field of animal husbandry diagnostic reagents, can solve the problems of low antigen content and inability to make early diagnosis, etc.

Inactive Publication Date: 2007-03-21
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the shortcoming that the amount of antigen obtained from parasites is small and cannot be used for early diagnosis when preparing indirect hemagglutination reagents with parasite antigens and membrane antigens, and to provide a kind of T. Indirect hemagglutination diagnostic reagent and preparation method thereof, the indirect hemagglutination diagnostic reagent can be used for the detection of toxoplasmosis and the general survey of toxoplasmosis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] On June 5, 2004, 20 mice with a body weight of 20-25 grams were inoculated intraperitoneally with 1×10^6 Toxoplasma gondii tachyzoites. On June 8, the mice showed symptoms such as depression and rough coat , put the mice to death by taking off their necks, and immerse them in 70% alcohol for surface disinfection. Each mouse was washed with 2 ml of PH7.2 PBS buffer solution to collect worms. A total of 45ml of peritoneal fluid was collected, and the collected peritoneal fluid was centrifuged at a speed of 3,000 rpm for 40 minutes to collect the supernatant, which was placed in a -20°C refrigerator. On June 10, the supernatant was taken out from the refrigerator and melted before Centrifuge at a speed of 4000 rpm for 3 times, each time for 30 minutes, collect the supernatant, and detect that the protein content of the supernatant is 21.37 mg / ml. The supernatant is the antigen secreted by Toxoplasma gondii metabolism. On June 11, the Toxoplasma gondii metabolism and secr...

Embodiment 2

[0017] On November 13, 2004, 20 mice weighing 20-25 grams were inoculated intraperitoneally with 1 × 10^5 Toxoplasma gondii tachyzoites. On November 17, the mice showed symptoms such as depression and rough coat , the mice were killed by decapitation, soaked in 75% alcohol for body surface disinfection. Each mouse was washed with 3 milliliters of PH7.2 PBS buffer solution to collect worms, and a total of 45 ml of peritoneal fluid was collected. The collected peritoneal fluid was centrifuged at a speed of 4000 rpm for 30 minutes, and the supernatant was collected and placed at -15 In the refrigerator at ℃, the supernatant was taken out from the refrigerator on November 18, melted and then centrifuged twice at 3000 rpm for 40 minutes each time, and the supernatant was collected. The protein content of the supernatant was detected to be 18.26mg / ml. The supernatant is the antigen secreted by Toxoplasma gondii metabolism. On November 19, the Toxoplasma gondii metabolism and secret...

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Abstract

The insect indirect blood diagnosis regent and its making method are made by the antigen secreted by the bow form insect. It inoculates the 1*10-5-1*10-6 bow form insect into the small rat belly cavity, then to soak in the alcohol to disinfect the body surface after making the small white rat death to collect a belly cavity liquid by the centrifugation; the red cell treated by the secreted antigen which is the bow insect indirect blood diagnosis antigen; the 5% red cell treated by the secreted antigen are suspended in the water bath box, then to centrifugate and wash for 3-5 times; then to wash by the 2% NRS of 0.15M PBS with the pH7.2 for 2-3 times. the sediments is to matched to the 1% sensitized red cell suspension with the 0.15M PBS of pH7.2 containing 2% NRS. It is dried by the freeze drier and vacuumize to get the indirect blood diagnosis regent with the IHA secreted by the bow form insect. The invention can be used for examining the bow form insect disease and the census aspect of bow form insect disease.

Description

technical field [0001] The invention relates to the field of animal husbandry diagnostic reagents, in particular to a toxoplasma gondii indirect hemagglutination diagnostic reagent, and also relates to a preparation method of the reagent. Background technique [0002] Toxoplasma gondii IHA indirect hemagglutination diagnostic reagent, as a means of detecting toxoplasmosis, has been widely used in my country and has made a significant contribution to the development of animal husbandry in my country. Currently available diagnostic reagents for toxoplasma gondii indirect hemagglutination can be divided into: (1) toxoplasma gondii antigen IHA indirect hemagglutination diagnostic reagent; (2) toxoplasma gondii membrane antigen IHA indirect hemagglutination diagnostic reagent. When the indirect hemagglutination reagent is prepared with insect body antigen and membrane antigen, the amount of antigen obtained from the insect body is small, and it cannot be used for early diagnosis....

Claims

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Application Information

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IPC IPC(8): G01N33/531G01N33/569
Inventor 张德林李学瑞王艳华李惠萍杜重波
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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