Total design scheme of automatic hemagglutination inhibition experiment workstation and application of total design scheme

A technology of hemagglutination inhibition and overall design, applied in the field of medical biology experiments

Pending Publication Date: 2017-08-25
陈凡
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is: Aiming at HI experiments, an overall design scheme of an automated HI detection workstation is proposed, which is used for large-scale sample detection, reduces manual operations, and reduces labor intensity

Method used

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  • Total design scheme of automatic hemagglutination inhibition experiment workstation and application of total design scheme
  • Total design scheme of automatic hemagglutination inhibition experiment workstation and application of total design scheme
  • Total design scheme of automatic hemagglutination inhibition experiment workstation and application of total design scheme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Determination of Antibody Levels in Serum of Avian Influenza with Hemagglutination Inhibition Experimental Workstation

[0033] (Take the hemagglutination inhibition experimental workstation equipped with 12-channel liquid distribution head as an example)

[0034] 1 Experimental materials

[0035] 1.1 HI experiment reaction solution (diluent), 1% chicken red blood cell suspension, prepared according to routine experiment procedures.

[0036] 1.2 Avian influenza hemagglutinin antigen, dissolved according to the instructions, diluted to a concentration of 4HA units for later use.

[0037] 1.3 96-well micro-hemagglutination reaction plate with a 90°V-shaped bottom.

[0038] 2 Set the working parameters of the workstation:

[0039] 2.1 Reaction solution distribution module: the volume is 25 μL per well.

[0040] 2.2 Sample ratio dilution module: pipette 6 times to mix, and the pipetting volume is 25 μL per well.

[0041] 2.3 Antigen distribution module: the v...

Embodiment 2

[0057] Example 2 Determination of avian influenza virus hemagglutination titer with hemagglutination inhibition experiment workstation

[0058] (Take a workstation equipped with a 12-channel liquid distribution head as an example)

[0059] 1 Experimental materials:

[0060] The avian influenza hemagglutinin antigen was used as the sample to be detected. Other experimental materials are the same as in Example 1.

[0061] 2 Set working parameters

[0062] Antigen distribution module, discontinued. Other operating parameters are the same as in Embodiment 1.

[0063] 3 Sample preparation:

[0064] The sample is placed in the first column of the 96-well microcoagulant plate, and the method is the same as in Example 1.

[0065] 4 Hemagglutination (HA) test

[0066] The process is similar to the hemagglutination inhibition test in Example 1. However, the two steps 4.3.3 and 4.3.4 are not required.

Embodiment 3

[0067] Example 3 Indirect hemagglutination test (IHA) of O-type foot-and-mouth disease antibody with hemagglutination inhibition experiment workstation

[0068] (Take a workstation equipped with a 12-channel liquid distribution head as an example)

[0069] 1 Experimental materials

[0070] 1.1 The IHA experimental reaction solution was prepared according to the experimental operating procedures.

[0071] 1.2 O-type foot-and-mouth disease hemagglutination antigen, positive control serum, and negative control serum were purchased from the National Foot-and-Mouth Disease Reference Laboratory and used according to the instructions.

[0072] 1.3 Serum to be tested is 0.5ml each, inactivated in a water bath at 56°C for 30 minutes.

[0073] 1.4 96-well micro-hemagglutination reaction plate, V-shaped at the bottom of 110°.

[0074] 2 Set the working parameters:

[0075] 2.1 Reaction solution distribution module: the volume is 50 μL per well.

[0076] 2.2 Sample ratio dilution mod...

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Abstract

The invention discloses a total design scheme of an automatic workstation for a large scale of hemagglutination inhibition (HI) experiments, and application of the design scheme in development of hemagglutination inhibition experiment special instruments and detection of the hemagglutination inhibition experiments. The workstation is a special instrument designed for specific steps of the HI experiments, and no similar products exist before. Compared with a PCR and an ELISA workstation, the workstation can meet the specific requirements of the HI experiments and can conduct HI detection on a large scale; compared with ordinary manual operation HI experiments, detection efficiency can be improved, labor cost can be saved, and working strength can be reduced. The workstation can detect the HI antibody titer of human and animal avian influenza, Newcastle disease, adenovirus and the like, can also be used for detecting the indirect hemagglutination (IHA) antibody titer of the foot-and-mouth disease, swine fever and the like and has good application prospects in all stages of laboratories.

Description

technical field [0001] The invention belongs to the technical field of medical biology experiments, and relates to an overall design scheme of a workstation for large-scale hemagglutination inhibition (HI) experiments. The workstation is designed for the specific steps of HI experiments, and consists of five working modules including reaction solution distribution, sample dilution, antigen distribution, red blood cell distribution, and result recording, as well as a base with a micro-coagulation plate delivery function. The workstation can detect HI antibody titers of human and animal avian influenza, Newcastle disease, adenovirus, etc. in batches, and can also be used to detect indirect hemagglutination (IHA) antibody titers of foot-and-mouth disease, swine fever, etc. Background technique [0002] Hemagglutination (HA) and hemagglutination inhibition (HI) assays are commonly used immunological assay techniques to detect antibody titers to viruses with hemagglutination acti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00G01N33/86
CPCG01N33/86G01N35/00
Inventor 陈凡
Owner 陈凡
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