Preparation method and detection method of goat pox reverse indirect hemagglutination diagnostic reagent

Abstract

The invention discloses a preparation method and a detection method of a goat pox reverse indirect hemagglutination diagnostic reagent capable of detecting goat pox antigen. The preparation method is: mix glutaraldehyde and sheep red blood cells to prepare red blood cell suspension, mix with the same amount of purified goat pox IgG, stir, centrifuge, wash, and use PBS containing 1% newborn calf serum to make 1% sensitization Red blood cells, this is the diagnostic reagent for goat pox reverse indirect hemagglutination. The optimized preparation conditions are: use glutaraldehyde monoformylation method to fix red blood cells, the concentration of sensitized red blood cells is 0.8%-1.0%, and the IgG content is controlled at 1.5-1.55mg.ml-1, which can achieve a better labeling effect; The reagent is used to detect goat pox virus antigen according to the conventional reverse indirect hemagglutination test method, the reaction temperature is 20-25°C, and the reaction time is 50min. The method has good specificity, sensitivity and repeatability, and can be used for rapid detection of goat pox antigen in veterinary clinic.

Description

technical field [0001] The invention relates to a preparation method and detection method of a goat pox reverse indirect hemagglutination diagnostic reagent. Background technique [0002] Goat pox is an endemic highly contagious disease caused by goat poxvirus (GTPV). It can occur throughout the year, common in spring and autumn, regardless of gender, age, or variety. The World Organization for Animal Health (OIE) listed goat pox as a major infectious disease of category A, and in 1999, the Ministry of Agriculture of the People's Republic of China announced goat pox as a national first-class animal disease. In recent years, more than 20 provinces and cities in my country have successively reported the occurrence of goat pox epidemic, which has caused great economic losses and posed a serious threat to the booming goat breeding industry in my country. [0003] The commonly used detection method of agar diffusion test in the past has the disadvantages of low sensitivity, slo...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a preparation method and detection method of a goat pox reverse indirect hemagglutination diagnostic reagent. The goat pox reverse indirect hemagglutination diagnostic reagent can quickly and accurately detect goat pox antigen, The indirect hemagglutination test has higher sensitivity and faster detection speed. It is used for the rapid detection of goat pox virus antigen in veterinary clinics, and provides a reference for the clinical diagnosis, prevention and control of goat pox, so as to overcome the low sensitivity and The detection speed is slow, the specificity is not strong, etc.

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