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45 results about "Sheep red blood cell" patented technology
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Kit for combined detection of 2019 novel coronavirus IgM and IgG antibodies and preparation method thereof
ActiveCN111426831AImprove structural stabilityHigh sensitivityBiological material analysisAgainst vector-borne diseasesIgm antibodyErythroid cell
The invention relates to the technical field of in-vitro diagnostic reagents, in particular to a kit for combined detection of novel 2019 coronavirus IgM and IgG antibodies and a preparation method ofthe kit. The kit comprises a duplex detection card and a detection buffer solution; a 2019 novel coronavirus IgM antibody detection test strip and a 2019 novel coronavirus IgG antibody detection teststrip are arranged in the duplex detection card; the 2019 novel coronavirus IgM antibody detection test strip and the 2019 novel coronavirus IgG antibody detection test strip respectively comprise asample pad, a nitrocellulose membrane and a water absorption pad, wherein the sample pad is coated with an anti-sheep erythrocyte antibody, and the nitrocellulose membrane is provided with a fluorescent antibody solidus, a detection line and a quality control line. By using the kit, the detection rate can be improved, and the operation is simple.
Owner:山东康华生物医疗科技股份有限公司 +1
Mycoplasma capricolum subsp. pneumonia antigen of goats and preparation method thereof
ActiveCN101712971AAvoid timeAvoid the disadvantage of low sensitization potencyBiological testingFermentationRed blood cellSubspecies
A mycoplasma capricolum subsp. pneumonia polysaccharide antigen of goats is characterized by extracting polysaccharides secreted by thalli from the supernatant of a mycoplasma capricolum subsp. pneumonia culture of goats. The agent is prepared by the following processing steps: a. culturing mycoplasma and b. extracting mycoplasma polysaccharide antigen. The invention has the following advantages:(1) the antigen in the invention is the polysaccharide antigen extracted from the supernatant of the mycoplasma capricolum subsp. pneumonia culture of goats and is polysaccharide secreted by thalli, has strong specificity and can truly detect the mycoplasma capricolum subsp. pneumonia antibody of goats; (2) the aldehyde-tanned sheep red cells sensitized with polysaccharide are used, thus avoidingthe defects of short preservation time and low sensitization titer of the fresh sheep red cells, being easily preserved for long time and having high hemagglutination titer;(3) the method is simple, convenient and fast in operation and dispenses with special equipment and apparatuses, thus being suitable for popularization and application at the grassroots level; (4) the invention is the unique diagnostic reagent for detecting the mycoplasma capricolum pneumonia antibodies of goats at home at present.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Novel Miltenberger blood group antibody detecting method
ActiveCN103344772ASolve the problem of shortage of sourcesSolve Simultaneous DetectionBiological testingFluorescenceMagnetization
The invention discloses a non-blood red cell dependent type Miltenberger blood group antibody detecting method. The detecting method comprises the following steps of: coupling sheep red blood cell subjected to hydroformylation and magnetization with polypeptide antigen containing a connecting chain to form an artificial screening cell; and uniformly mixing the artificial screening cell with a detecting sample for cultivating and washing, and measuring the result after adding fluorescence-labeled secondary antibody for cultivating and washing. By virtue of the way, the non-blood red cell dependent type Miltenberger blood group antibody detecting method provided by the invention is free of centrifuging for the washing due to the application of magnetic force, simple and convenient to operate, capable of realizing simultaneously detecting complete antibody and incomplete antibody, short in detecting process time consumption, easy to automate, accurate in detected result and capable of realizing semi-quantitative or quantitative detection by combining with a detecting apparatus, and the result is easy to judge. Besides, the detecting method can guarantee safe, efficient and scientific blood transfusion in clinic.
Owner:SUZHOU GUOKE MEDICAL TECH DEV CO LTD
Preparation of duck hepatitis I type virus indirect hemagglutination diagnostic antigen and kit
InactiveCN101423548AQuick responseEasy to useVirus peptidesMaterial analysisDuck hepatitis A virusType antigen
The invention relates to a method for preparing Duck hepatitis virus I type indirect blood coagulation diagnosing antigen, in particular to a method for preparing indirect blood coagulation diagnosing antigen by Duck hepatitis virus I type antigen sensibilization double hydroformylation mutton red cell. The Duck hepatitis virus I type indirect blood coagulation diagnosing antigen produced by the method has long storage time up to half a year; moreover, the diagnosing time is shortened and only half an hour is needed, and the use is convenient and simple; therefore, the method is suitable to be popularized and applied to the practical production.
Owner:陶海静
Sperm antibody testing reagent
The sperm antibody testing reagent includes sensitized sheep erythrocyte suspension of 2-3 % concentration and rabbit antihuamn IgG in the concentration of 1 to 2 to the (8-10)-th power. The sensitized sheep erythrocyte suspension of 2-3% concentration is prepared through taking sheep blood, washing, hydroformylation, tanning, sensitizing and other steps. The preparation of rabbit antihuman IgG in the concentration of 1 to 2 to the (8-10)-th power includes taking rabbit blood, separating blood serum and other steps. The reagent has strong specificity, high detection sensitivity, no false positive and no false negative phenomenon, high stability, and storage life as long as one year. Using the reagent can obtain easy-to-judge detection result sipmle and fast.
Owner:ANHUI ANKE BIOTECHNOLOGY (GRP) CO LTD
Method for preparing magnetized and hydroformyled sheep red blood cell
The invention discloses a method for preparing a magnetized and hydroformyled sheep red blood cell. The method comprises the following steps of: preparing nano magnetic beads; preparing a hydroformyled sheep red blood cell; preparing a magnetized and hydroformyled sheep red blood cell; coating protein on the magnetized and hydroformyled sheep red blood cell; detecting a sample; and observing the result. The prepared magnetized and hydroformyled sheep red blood cell can be used as an indicator cell of an agglutination test, a carrier, enzyme and a chemiluminescence matter of an antigen antibody agglutination test, or a carrier detected in a fluorescent material marking test. The magnetized and hydroformyled sheep red blood cell prepared by the method is long in retention period, simultaneously has paramagnetism, can achieve rapid aggregation and redispersion, avoids complicated centrifuge processes in the traditional agglutination test, and can be used for preparing artificial granular antigen antibodies with uniform sizes; coating and detection reaction are carried out in a uniform phase after a solid-phase carrier is replaced; and the method is full in action, high in sensitivity, low in cost than an elisa plate, and is mainly applied to a screening or quantitative detection test of a special red blood cell antibody and other soluble antigen antibodies.
Owner:SUZHOU GUOKE MEDICAL TECH DEV CO LTD
Application of verbascoside in preparation of pneumonia treatment drug
The invention relates to application of verbascoside in preparation of a pneumonia treatment drug. The treatment effect of the verbascoside to streptococcus pneumoniae infection is proved through sheep red blood cell hemolysis test, protection experiment of the human pulmonary epithelial cell (A549) damage and a mouse streptococcus pneumoniae pneumonia pneumonia model. Compared with antibiotic treatment, when the verbascoside is used for treatment, so that the pneumonia treatment drug has the advantages that no drug resistance exists and the curing rate is high.
Owner:JILIN UNIV
Application of three kinds of oroxin in preparing drug for resisting staphylococcus aureus infection
The invention relates to application of three kinds of oroxin in preparing a drug for resisting staphylococcus aureus infection. The staphylococcus aureus infection resistance of three kinds of oroxin can be proved by virtue of a sheep red blood cell haemolysis test, molecular dynamics simulation and a human pulmonary epithelial cell (A549) injury protective test. Compared with treatment by antibiotics, treatment by using three kinds of oroxin has the characteristics of no drug resistance and high cure rate, so that the three kinds of oroxin can be used in development of new drugs, and has an important meaning to drug target confirmation.
Owner:JILIN UNIV
Staphylococcus Warner indirect blood coagulation detection kit and application thereof
The invention provides a staphylococcus Warner indirect blood coagulation detection kit and application thereof. The kit is characterized by comprising a staphylococcus Warner indirect blood coagulation antigen, wherein the staphylococcus Warner indirect blood coagulation antigen is composed of whole bacterial protein of staphylococcus Warner CCTCC M2016048 and hydroformylated-tanned sheep red blood cells. The kit provided by the invention is simple to operate and is convenient and rapid, and special equipment and instruments are not needed; and a whole process is finished within 2-3 hours, so that the kit is suitable for being popularized and applied to a basic level and can be widely applied to investigation and study of staphylococcus Warner disease epidemiology. A detection result has good stability and is stable and reliable, and an antibody of staphylococcus Warner can be accurately detected.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
CD4+T cell derived exosomes and application thereof
ActiveCN107365742APromote proliferationPromote productionViral antigen ingredientsDigestive systemImmune effectsAdjuvant
The invention realtes to CD4+T cell derived exosomes and application thereof, and belongs to the field of cytobiology, molecular biology and clinical application. The invention particularly discloses CD4+T cell derived exosomes (named as CD4+ T exo) and application thereof in preparation an adjuvant for enhancing a vaccine immunity effect. It discovers that CD4+ T exo can effectively promote proliferation and activation of B cells in vitro, and can promote the B cells to produce more antibodies. Meanwhile, CD4+ T exo can serve as an adjuvant to enhance the immune effect of sheep red blood cell (SRBC) immunized mice in vivo, can also have obvious enhancing effect on hepatitis B vaccine immunized Babl / c mice, and promotes peripheral blood to produce more hepatitis B surface antibodies (HbsAb). The application of the CD4+T cell derived exosomes can be used for preparing a novel adjuvant for enhancing the antigen / vaccine immunization effect.
Owner:JIANGSU UNIV
Kit for detecting indirect hemagglutination of streptococcus suis and application thereof
ActiveCN106093439AAvoid timeAvoid the disadvantage of low sensitization potencyBiological testingMicrobiologyStreptococcus mitis
The invention provides a kit for detecting indirect hemagglutination of streptococcus suis. The kit comprises indirect hemagglutination antigen of streptococcus suis, and the indirect hemagglutination antigen of streptococcus suis is composed of whole bacterial protein of streptococcus suis CCTCC M 2016028 and aldehydated-tanned sheep red blood cell. The kit has the advantages that the operation is simple, convenient and rapid, no special equipment and apparatus is required, a whole course can be completed in 2-3 hours, the kit is suitable for basic popularization and application, and can be widely used for investigation and research of epidemiology of swine streptococcosis. The results have the advantages of good repeatability, stabilization, and reliablility, and can accurately detect the antibody of streptococcus suis.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Haemophilus parasuis indirect hamagglutination detection reagent
The invention relates to the field of veterinary detection reagents, and discloses a haemophilus parasuis indirect hamagglutination detection reagent. The haemophilus parasuis indirect hamagglutination detection reagent is prepared by taking multiple serotype haemophilus parasuis whole protein mixtures as antigens, and is capable of rapidly and sensitively detecting serum antibodies of all serotype haemophilus parasuis diseases. The production technology is optimized through balanced mixing of each whole protein antigen, and then sensibilization and double hydroformylation of mutton red cells; and the prepared haemophilus parasuis indirect hamagglutination detection reagent is stable, sensitive, good in specificity, long in storage life, simple in operation, and applicable to a large amount of clinical sample detection on the haemophilus parasuis diseases, immune level detection and epidemiology investigation.
Owner:JIANGSU ACAD OF AGRI SCI
Detection kit for enterococcus faecium indirect blood clotting and application thereof
The invention provides a detection kit for enterococcus faecium indirect blood clotting. The detection kit is characterized by comprising an enterococcus faecium indirect blood clotting antigen, wherein the enterococcus faecium indirect blood clotting antigen is composed of enterococcus faecium CCTCC M 2016047 whole bacterial proteins and hydroformylation-tannin sheep red blood cells. The kit provided by the invention is simple, convenient and quick in operation; special equipment and instrument are not required; the whole process can be completed within 2-3 hours; the detection kit is fit for popularization and application in basic level; the detection kit can be widely applied to the investigation of enterococcus faecium epidemiology; the repeatability of the result is excellent; the result is stable and reliable; the antibody of the enterococcus faecium can be accurately detected.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Use of winged euony twigs in preparing anti-IV type allergy medicine
The invention relates to the medical technique field and discloses an application for preparing IV-type allergic response resistance medicament of an extract of ramulus euonymi which is a celastraceae plant. The animal models (sheep red blood cell causing mice delayed type pedal swelling, olive oil causing mice dermatitis contacta, 5-HT causing rat mastocyte in abdominal cavity releasing histamine, 5-HT inducing guinea pig ileum shrinking) which are broadly used for screening IV-type allergic response resistance in at home and abroad at present, verifies that the extract of the ramulus euonymi can obviously inhibit the mice delayed type pedal swelling caused by sheep red blood cells, the mice dermatitis contacta caused by olive oil, the rat mastocyte in abdominal cavity releasing histamine caused by 5-HT and the guinea pig ileum shrinking caused by 5-HT, thereby proving that the ramulus euonymi has exact effect in resisting IV-type allergic response, and therefore the ramulus euonymi can be used for preparing IV-type allergic response resistance medicament.
Owner:黄德斌 +2
Goat pox reverse indirect blood coagulation diagnostic reagent, and preparation method, and detection method thereof
InactiveCN102253197AQuick checkAccurate detectionMaterial analysisSheep red blood cellSensitized red cell
The invention discloses a goat pox reverse indirect blood coagulation diagnostic reagent and a preparation method thereof. The content of sensitized erythrocyte in the blood coagulation reagent is 0.8 to 1.0 percent; and the purified goat pox IgG content is 1.549mg / ml. Compared with the conventional common agar diffusion experiment, the preparation method has the advantages that: the goat pox reverse indirect blood coagulation diagnostic reagent is prepared by sensitizing hydroformylation sheep red blood cells by using the purified goat pox IgG, so that a goat poxvirus antibody can be detected quickly, accurately and simply; and the goat pox reverse indirect blood coagulation diagnostic reagent has higher specificity, sensitivity and repeatability and can be widely applied to quick veterinary clinical detection of the goat poxvirus antibody and provide a basis for disease prevention, diagnosis and control.
Owner:GUIZHOU UNIV
Application of juglone for adjusting immune states of mice and oxidizing reactions
InactiveCN102824335AEnhance humoral immunityRegulate immune system functionOrganic active ingredientsAntinoxious agentsOrganismImmunologic function
The invention relates to the technical field of medicines, discovers an application of juglone for adjusting immune states of mice and oxidizing reactions, and mainly researches medicinal composition juglone. A chemical name of the juglone is 5-hydroxy-1,4-naphthoquinone and 5-hydroxy-1,4-naphthalene diketone. An English name of the juglone is Juglone, walnuts quinine, 5-hydroxy-1,4-naphthoquinone and 5-hydroxynaphthalene, regianin, Nucin. A molecular formula of the juglone is C10H6O3. A relative molecular weight is 174.15. An immunocompromised model is built through systematic pharmacological tests and by using KM mice and adopting dexamethasone sodium phosphate injecta, a mouse immunostimulation model is built by using sheep red blood cells (SRBC) to induce the mice to produce SRBC immune bodies and cyclophosphamide, a fact that the juglone can recover low immunologic functions to a normal level is proved, the immunostimulation states are improved, self stability of an organism is maintained, and both-way immunoregulation functions are displayed. The juglone displays functions of adjusting organism immune system functions by adjusting oxidative stress reactions in the organism.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY
Preparation method and detection method of goat pox reverse indirect hemagglutination diagnostic reagent
InactiveCN102288756AThe detection process is fastIncreased sensitivityMaterial analysisRed blood cellReaction temperature
The invention discloses a preparation method and a detection method of a goat pox reverse indirect hemagglutination diagnostic reagent capable of detecting goat pox antigen. The preparation method is: mix glutaraldehyde and sheep red blood cells to prepare red blood cell suspension, mix with the same amount of purified goat pox IgG, stir, centrifuge, wash, and use PBS containing 1% newborn calf serum to make 1% sensitization Red blood cells, this is the diagnostic reagent for goat pox reverse indirect hemagglutination. The optimized preparation conditions are: use glutaraldehyde monoformylation method to fix red blood cells, the concentration of sensitized red blood cells is 0.8%-1.0%, and the IgG content is controlled at 1.5-1.55mg.ml-1, which can achieve a better labeling effect; The reagent is used to detect goat pox virus antigen according to the conventional reverse indirect hemagglutination test method, the reaction temperature is 20-25°C, and the reaction time is 50min. The method has good specificity, sensitivity and repeatability, and can be used for rapid detection of goat pox antigen in veterinary clinic.
Owner:GUIZHOU UNIV
Nutrient medium for cultivating bacteria
The invention relates to microbiology, and more particularly to nutrient media used for cultivating bacteria for the subsequent study thereof. A nutrient medium comprising a pancreatic digest of casein, a peptic digest of meat, a heart pancreatic digest, yeast extract, starch and water is characterized in that it additionally contains violuric acid and beef infusion, wherein the ratio of ingredients is (wt %):0.3-1.0 pancreatic digest of casein; 0.1-1.5 peptic digest of meat; 0.1-0.9 heart pancreatic digest; 0.1-2.0 yeast extract, 0.3-0.8 starch; 0.001-0.05 violuric acid; 2.0-15 beef infusion; the remainder water. The nutrient medium can additionally contain 0.3-2.5 wt % agar-agar and / or 1-20 wt % whole or hemolyzed sheep red blood cells and / or 1-20 wt % whole or hemolyzed human red blood cells and / or 1-15 wt % horse blood serum. This provides for the simultaneous growth of the maximum possible number of bacteria present in an inoculate.
Owner:VIKTOR VENIAMINOVICH TETAB +1
Flavanone glycoside and its preparation and use
InactiveCN1594346AGood inhibitory effectEnhanced inhibitory effectOrganic active ingredientsSugar derivativesImmunologic disordersDisease
The invention provides a flavanones group glycoside compounds having the structural formula disclosed in the specification, the compound has both appreciable inhibitory action to mixed lymphocyte reaction, apoptosis inducement activity to splenocyte activated by 2,4,6-trinitrochlorobenzene and sheep red blood cells, and evident inhibitory action for the splenocyte NO secretion by PC1-CS model mouse. The invention also discloses the process of preparation.
Owner:NANJING UNIV
Goat pox indirect hemagglutination diagnosis reagent as well as preparation method and detecting method thereof
InactiveCN102313803AQuick checkAccurate detectionMaterial analysisRed blood cellIndirect hemagglutination
The invention discloses a goat pox indirect hemagglutination diagnosis reagent as well as a preparation method and a detecting method thereof. The hemagglutination diagnosis reagent comprises the following components by weight percent: 0.6-1.2% of goat pox virus antigen sensitized red blood cells, and 20-200 mu g / ml of purified goat pox virus antigen. Compared with the commonly used agar diffusion experiment, the goat pox indirect hemagglutination diagnosis reagent is prepared from purified goat pox virus antigen sensitized hydroformylated sheep red blood cells in the invention, thus, the goat pox virus antibody can be detected fast, accurately and simply, the result can be determined in 50 minutes, and the antibody with low valence can be detected; moreover, the goat pox indirect hemagglutination diagnosis reagent has good specificity, sensitivity and repeatability, and can be widely used for fast and substantive detection of goat pox virus antibodies in clinical vet, and can provide the basis for prevention, diagnosis and prevention and control of a disease.
Owner:GUIZHOU UNIV
Preparation method of Gandakang capsules
ActiveCN102335381AGuarantee product qualityReduce damageAnthropod material medical ingredientsDigestive systemTreatment effectRed blood cell
The invention relates to a preparation method of Gandakang capsules, and belongs to the preparation method of traditional Chinese medicines. According to the invention, after a new process is determined, through study and dynamic detection of three batches of pilot-scale products, the character identification of the product and thin layer identification of four medical materials are determined, and an HPLC (high-performance liquid chromatography) measuring method for peoniflorin content is also built; and according to the above quality standards, the feeding process, and processing steps from the intermediate processing to finished product processing are effectively controlled in the improved production process of the Gandakang capsules, thus the product quality is guaranteed. The Gandakang capsules has the effects of obviously resisting aminotransferase increase caused by CCl4 and D-galactosamine, relieving the degree of liver injury caused by chemical substances, and protecting liver cell membrane; and the Gandakang capsules also has the effects of improving phagocytosis function of mice and the generation of antibody for mouse hemolysin caused by sheep erythrocyte, thus the product can better improve mouse immune function, and the new dosage form of Gandakang capsules has good liver protection effect and has better treatment effect if applied clinically.
Owner:吉林敖东生物科技股份有限公司
A kind of Mycoplasma bovis antibody detection reagent and preparation method thereof
ActiveCN104062439BDetect trueReliable detectionDepsipeptidesBiological testingMycoplasma antibodyBlood coagulations
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Application of fisetin in the preparation of anti-listeria infection drugs
The invention relates to application of fisetin in preparation of a drug for resisting listeria infection. It is proved that the drug has the effect of resisting listeria infection by a sheep red blood cell hemolysis test, a protection test of mouse macrophage-like cell J774 and mouse peritoneal macrophages damage, and a mouse listeria infection model. Compared with treatment by antibiotic, treatment by fisetin has the characteristics of being free of drug tolerance and high in recovery rate. Therefore, fisetin can be used for developing a new drug, and has significance on confirming of drug targets.
Owner:SHANDONG GUANGYUAN PHARM SCI & TECH CO LTD +1
Flavanone glycoside and its preparation and use
InactiveCN100457767CEnhanced inhibitory effectOrganic active ingredientsSugar derivativesImmunologic disordersDisease
The invention provides a flavanones group glycoside compounds having the structural formula disclosed in the specification, the compound has both appreciable inhibitory action to mixed lymphocyte reaction, apoptosis inducement activity to splenocyte activated by 2,4,6-trinitrochlorobenzene and sheep red blood cells, and evident inhibitory action for the splenocyte NO secretion by PC1-CS model mouse. The invention also discloses the process of preparation.
Owner:NANJING UNIV
Haemophilus parasuis indirect hamagglutination detection reagent
The invention relates to the field of veterinary detection reagents, and discloses a haemophilus parasuis indirect hamagglutination detection reagent. The haemophilus parasuis indirect hamagglutination detection reagent is prepared by taking multiple serotype haemophilus parasuis whole protein mixtures as antigens, and is capable of rapidly and sensitively detecting serum antibodies of all serotype haemophilus parasuis diseases. The production technology is optimized through balanced mixing of each whole protein antigen, and then sensibilization and double hydroformylation of mutton red cells; and the prepared haemophilus parasuis indirect hamagglutination detection reagent is stable, sensitive, good in specificity, long in storage life, simple in operation, and applicable to a large amount of clinical sample detection on the haemophilus parasuis diseases, immune level detection and epidemiology investigation.
Owner:JIANGSU ACAD OF AGRI SCI
Detection kit, preparation method and application of hemagglutination-inhibiting antibody of Aviobacterium gallinarum
The invention discloses a detection kit for detecting ornithobacterium rhinotracheale hemagglutinin inhibitor antibody, a preparation method, and applications thereof, and especially relates to a detection kit for detecting hemagglutinin inhibitor antibody of the ornithobacterium rhinotracheale YN12091 whose bacterial strain preservation number is CCTCC No: M2013318, being named ORT for short, a preparation method, and applications thereof. The preparation method comprises following steps: choosing ORT high hemagglutinin activity bacterial strains, culturing and washing to prepare ORT hemagglutinin antigens; collecting sheep blood, preparing sheep erythrocytes from the collected sheep blood through a washing and centrifugation method, fixing the sheep erythrocytes with glutaraldehyde and then tanning the sheep erythrocytes with tannic acid so as to prepare hydroformylated sheep erythrocytes; immunizing chickens for three times with an ORT bacterial suspension, then preparing ORT standard positive serum through centrifugation of the collected blood; assembling the prepared ORT hemagglutinin antigens, hydroformylated sheep erythrocytes, and ORT standard positive serum to establish a detection kit for detecting ornithobacterium rhinotracheale hemagglutinin inhibitor antibody. The kit not only can carry out detections for ORT antibodies in chicken serum, but also can carry out detections for yolk antibodies in chicken egg yolks.
Owner:YUNNAN ANIMAL SCI & VETERINARY INST
Determining method capable of quantitatively determining activity of bacterial secretory hemolysin
InactiveCN107036866APreparing sample for investigationColor/spectral properties measurementsExperimental methodsHemolysis
The invention belongs to the technical field of design of biological experimental methods, and particularly relates to a determining method capable of quantitatively determining activity of bacterial secretory hemolysin. The determining method comprises the following steps: first, reagent and preparation: (1) preparation of hemocyte storing liquid; (2) preparation of buffering saline and storing liquid, and preparation of buffering liquid; (3) preparation and storage of 1% sheep red blood cells; and (4) preparation and storage of hemolysin to be measured; and second, determination and operation by a test tube method: (1) diluting of the hemolysin; (2) preparation of a hemolysis standard tube; (3) hemolysis contrast; and (4) result determination; or second, determination and operation by a micro-pore method: (1) diluting of the hemolysin; (2) preparation of a hemolysis standard hole; (3) emolysis contrast; and (4) result determination. By the determining method, bacterial hemolysin can be determined well quantitatively.
Owner:新疆畜牧科学院
Novel Miltenberger blood group antibody detecting method
ActiveCN103344772BSolve the problem of shortage of sourcesSolve Simultaneous DetectionBiological testingFluorescenceMagnetization
The invention discloses a non-blood red cell dependent type Miltenberger blood group antibody detecting method. The detecting method comprises the following steps of: coupling sheep red blood cell subjected to hydroformylation and magnetization with polypeptide antigen containing a connecting chain to form an artificial screening cell; and uniformly mixing the artificial screening cell with a detecting sample for cultivating and washing, and measuring the result after adding fluorescence-labeled secondary antibody for cultivating and washing. By virtue of the way, the non-blood red cell dependent type Miltenberger blood group antibody detecting method provided by the invention is free of centrifuging for the washing due to the application of magnetic force, simple and convenient to operate, capable of realizing simultaneously detecting complete antibody and incomplete antibody, short in detecting process time consumption, easy to automate, accurate in detected result and capable of realizing semi-quantitative or quantitative detection by combining with a detecting apparatus, and the result is easy to judge. Besides, the detecting method can guarantee safe, efficient and scientific blood transfusion in clinic.
Owner:SUZHOU GUOKE MEDICAL TECH DEV CO LTD
Preparation method of liver protection capsules
ActiveCN102335381BIncrease inclusion processImprove exclusion rateAnthropod material medical ingredientsDigestive systemRed blood cellCell membrane
Owner:吉林敖东生物科技股份有限公司
Mycoplasma capricolum subsp. pneumonia antigen of goats and preparation method thereof
ActiveCN101712971BStrong specificityEasy to storeBiological testingFermentationRed blood cellSubspecies
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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