45 results about "Sheep red blood cell" patented technology

A mycoplasma capricolum subsp. pneumonia polysaccharide antigen of goats is characterized by extracting polysaccharides secreted by thalli from the supernatant of a mycoplasma capricolum subsp. pneumonia culture of goats. The agent is prepared by the following processing steps: a. culturing mycoplasma and b. extracting mycoplasma polysaccharide antigen. The invention has the following advantages:(1) the antigen in the invention is the polysaccharide antigen extracted from the supernatant of the mycoplasma capricolum subsp. pneumonia culture of goats and is polysaccharide secreted by thalli, has strong specificity and can truly detect the mycoplasma capricolum subsp. pneumonia antibody of goats; (2) the aldehyde-tanned sheep red cells sensitized with polysaccharide are used, thus avoidingthe defects of short preservation time and low sensitization titer of the fresh sheep red cells, being easily preserved for long time and having high hemagglutination titer;(3) the method is simple, convenient and fast in operation and dispenses with special equipment and apparatuses, thus being suitable for popularization and application at the grassroots level; (4) the invention is the unique diagnostic reagent for detecting the mycoplasma capricolum pneumonia antibodies of goats at home at present.

The invention discloses a non-blood red cell dependent type Miltenberger blood group antibody detecting method. The detecting method comprises the following steps of: coupling sheep red blood cell subjected to hydroformylation and magnetization with polypeptide antigen containing a connecting chain to form an artificial screening cell; and uniformly mixing the artificial screening cell with a detecting sample for cultivating and washing, and measuring the result after adding fluorescence-labeled secondary antibody for cultivating and washing. By virtue of the way, the non-blood red cell dependent type Miltenberger blood group antibody detecting method provided by the invention is free of centrifuging for the washing due to the application of magnetic force, simple and convenient to operate, capable of realizing simultaneously detecting complete antibody and incomplete antibody, short in detecting process time consumption, easy to automate, accurate in detected result and capable of realizing semi-quantitative or quantitative detection by combining with a detecting apparatus, and the result is easy to judge. Besides, the detecting method can guarantee safe, efficient and scientific blood transfusion in clinic.

The sperm antibody testing reagent includes sensitized sheep erythrocyte suspension of 2-3 % concentration and rabbit antihuamn IgG in the concentration of 1 to 2 to the (8-10)-th power. The sensitized sheep erythrocyte suspension of 2-3% concentration is prepared through taking sheep blood, washing, hydroformylation, tanning, sensitizing and other steps. The preparation of rabbit antihuman IgG in the concentration of 1 to 2 to the (8-10)-th power includes taking rabbit blood, separating blood serum and other steps. The reagent has strong specificity, high detection sensitivity, no false positive and no false negative phenomenon, high stability, and storage life as long as one year. Using the reagent can obtain easy-to-judge detection result sipmle and fast.

The invention discloses a method for preparing a magnetized and hydroformyled sheep red blood cell. The method comprises the following steps of: preparing nano magnetic beads; preparing a hydroformyled sheep red blood cell; preparing a magnetized and hydroformyled sheep red blood cell; coating protein on the magnetized and hydroformyled sheep red blood cell; detecting a sample; and observing the result. The prepared magnetized and hydroformyled sheep red blood cell can be used as an indicator cell of an agglutination test, a carrier, enzyme and a chemiluminescence matter of an antigen antibody agglutination test, or a carrier detected in a fluorescent material marking test. The magnetized and hydroformyled sheep red blood cell prepared by the method is long in retention period, simultaneously has paramagnetism, can achieve rapid aggregation and redispersion, avoids complicated centrifuge processes in the traditional agglutination test, and can be used for preparing artificial granular antigen antibodies with uniform sizes; coating and detection reaction are carried out in a uniform phase after a solid-phase carrier is replaced; and the method is full in action, high in sensitivity, low in cost than an elisa plate, and is mainly applied to a screening or quantitative detection test of a special red blood cell antibody and other soluble antigen antibodies.

The invention relates to application of verbascoside in preparation of a pneumonia treatment drug. The treatment effect of the verbascoside to streptococcus pneumoniae infection is proved through sheep red blood cell hemolysis test, protection experiment of the human pulmonary epithelial cell (A549) damage and a mouse streptococcus pneumoniae pneumonia pneumonia model. Compared with antibiotic treatment, when the verbascoside is used for treatment, so that the pneumonia treatment drug has the advantages that no drug resistance exists and the curing rate is high.

The invention provides a staphylococcus Warner indirect blood coagulation detection kit and application thereof. The kit is characterized by comprising a staphylococcus Warner indirect blood coagulation antigen, wherein the staphylococcus Warner indirect blood coagulation antigen is composed of whole bacterial protein of staphylococcus Warner CCTCC M2016048 and hydroformylated-tanned sheep red blood cells. The kit provided by the invention is simple to operate and is convenient and rapid, and special equipment and instruments are not needed; and a whole process is finished within 2-3 hours, so that the kit is suitable for being popularized and applied to a basic level and can be widely applied to investigation and study of staphylococcus Warner disease epidemiology. A detection result has good stability and is stable and reliable, and an antibody of staphylococcus Warner can be accurately detected.

The invention discloses a goat pox reverse indirect blood coagulation diagnostic reagent and a preparation method thereof. The content of sensitized erythrocyte in the blood coagulation reagent is 0.8 to 1.0 percent; and the purified goat pox IgG content is 1.549mg/ml. Compared with the conventional common agar diffusion experiment, the preparation method has the advantages that: the goat pox reverse indirect blood coagulation diagnostic reagent is prepared by sensitizing hydroformylation sheep red blood cells by using the purified goat pox IgG, so that a goat poxvirus antibody can be detected quickly, accurately and simply; and the goat pox reverse indirect blood coagulation diagnostic reagent has higher specificity, sensitivity and repeatability and can be widely applied to quick veterinary clinical detection of the goat poxvirus antibody and provide a basis for disease prevention, diagnosis and control.

The invention relates to the technical field of medicines, discovers an application of juglone for adjusting immune states of mice and oxidizing reactions, and mainly researches medicinal composition juglone. A chemical name of the juglone is 5-hydroxy-1,4-naphthoquinone and 5-hydroxy-1,4-naphthalene diketone. An English name of the juglone is Juglone, walnuts quinine, 5-hydroxy-1,4-naphthoquinone and 5-hydroxynaphthalene, regianin, Nucin. A molecular formula of the juglone is C10H6O3. A relative molecular weight is 174.15. An immunocompromised model is built through systematic pharmacological tests and by using KM mice and adopting dexamethasone sodium phosphate injecta, a mouse immunostimulation model is built by using sheep red blood cells (SRBC) to induce the mice to produce SRBC immune bodies and cyclophosphamide, a fact that the juglone can recover low immunologic functions to a normal level is proved, the immunostimulation states are improved, self stability of an organism is maintained, and both-way immunoregulation functions are displayed. The juglone displays functions of adjusting organism immune system functions by adjusting oxidative stress reactions in the organism.

The invention relates to microbiology, and more particularly to nutrient media used for cultivating bacteria for the subsequent study thereof. A nutrient medium comprising a pancreatic digest of casein, a peptic digest of meat, a heart pancreatic digest, yeast extract, starch and water is characterized in that it additionally contains violuric acid and beef infusion, wherein the ratio of ingredients is (wt %):0.3-1.0 pancreatic digest of casein; 0.1-1.5 peptic digest of meat; 0.1-0.9 heart pancreatic digest; 0.1-2.0 yeast extract, 0.3-0.8 starch; 0.001-0.05 violuric acid; 2.0-15 beef infusion; the remainder water. The nutrient medium can additionally contain 0.3-2.5 wt % agar-agar and/or 1-20 wt % whole or hemolyzed sheep red blood cells and/or 1-20 wt % whole or hemolyzed human red blood cells and/or 1-15 wt % horse blood serum. This provides for the simultaneous growth of the maximum possible number of bacteria present in an inoculate.

The invention provides a flavanones group glycoside compounds having the structural formula disclosed in the specification, the compound has both appreciable inhibitory action to mixed lymphocyte reaction, apoptosis inducement activity to splenocyte activated by 2,4,6-trinitrochlorobenzene and sheep red blood cells, and evident inhibitory action for the splenocyte NO secretion by PC1-CS model mouse. The invention also discloses the process of preparation.

The invention discloses a goat pox indirect hemagglutination diagnosis reagent as well as a preparation method and a detecting method thereof. The hemagglutination diagnosis reagent comprises the following components by weight percent: 0.6-1.2% of goat pox virus antigen sensitized red blood cells, and 20-200 mu g/ml of purified goat pox virus antigen. Compared with the commonly used agar diffusion experiment, the goat pox indirect hemagglutination diagnosis reagent is prepared from purified goat pox virus antigen sensitized hydroformylated sheep red blood cells in the invention, thus, the goat pox virus antibody can be detected fast, accurately and simply, the result can be determined in 50 minutes, and the antibody with low valence can be detected; moreover, the goat pox indirect hemagglutination diagnosis reagent has good specificity, sensitivity and repeatability, and can be widely used for fast and substantive detection of goat pox virus antibodies in clinical vet, and can provide the basis for prevention, diagnosis and prevention and control of a disease.

The invention relates to a preparation method of Gandakang capsules, and belongs to the preparation method of traditional Chinese medicines. According to the invention, after a new process is determined, through study and dynamic detection of three batches of pilot-scale products, the character identification of the product and thin layer identification of four medical materials are determined, and an HPLC (high-performance liquid chromatography) measuring method for peoniflorin content is also built; and according to the above quality standards, the feeding process, and processing steps from the intermediate processing to finished product processing are effectively controlled in the improved production process of the Gandakang capsules, thus the product quality is guaranteed. The Gandakang capsules has the effects of obviously resisting aminotransferase increase caused by CCl4 and D-galactosamine, relieving the degree of liver injury caused by chemical substances, and protecting liver cell membrane; and the Gandakang capsules also has the effects of improving phagocytosis function of mice and the generation of antibody for mouse hemolysin caused by sheep erythrocyte, thus the product can better improve mouse immune function, and the new dosage form of Gandakang capsules has good liver protection effect and has better treatment effect if applied clinically.