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Application of three kinds of oroxin in preparing drug for resisting staphylococcus aureus infection

A technology of staphylococcus aureus and three kinds of wood, applied in the field of medicine, can solve the problems of no cure for staphylococcus aureus disease and increased bacterial resistance rate

Inactive Publication Date: 2014-01-01
JILIN UNIV
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AI Technical Summary

Problems solved by technology

In recent years, the extensive use of antibiotics has led to an increase in the rate of bacterial drug resistance. It has been found that a variety of clinical isolates are highly resistant to antibiotics such as tetracycline and ciprofloxacin, resulting in the treatment of staph.

Method used

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  • Application of three kinds of oroxin in preparing drug for resisting staphylococcus aureus infection
  • Application of three kinds of oroxin in preparing drug for resisting staphylococcus aureus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0009] Hemolysis test

[0010] The purified Staphylococcus aureus α-hemolysin was serially diluted twice in a 96-well plate, and 10 7 Sheep red blood cells were incubated at 37°C for 30 h and then centrifuged. The highest dilution factor that lyses red blood cells is regarded as the hemolysis titre, such as figure 1 shown.

[0011] Confirmation of the active site of α-hemolysin of oleoside against Staphylococcus aureus by molecular dynamics simulation

[0012] 2.1 Confirmation of active sites by molecular dynamics simulation

[0013] (1) Molecular docking

[0014] In order to preliminarily determine the binding site of oreoside and α-hemolysin, as well as the strength of the intermolecular binding between oreoside and α-hemolysin, the molecular docking of oreoside and α-hemolysin was firstly carried out, and the Structure of complex with α-hemolysin.

[0015] (2) Molecular dynamics simulations

[0016] Using molecular dynamics simulation to study the change of bindi...

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Abstract

The invention relates to application of three kinds of oroxin in preparing a drug for resisting staphylococcus aureus infection. The staphylococcus aureus infection resistance of three kinds of oroxin can be proved by virtue of a sheep red blood cell haemolysis test, molecular dynamics simulation and a human pulmonary epithelial cell (A549) injury protective test. Compared with treatment by antibiotics, treatment by using three kinds of oroxin has the characteristics of no drug resistance and high cure rate, so that the three kinds of oroxin can be used in development of new drugs, and has an important meaning to drug target confirmation.

Description

technical field [0001] The invention relates to the application of three kinds of pterosides in the preparation of anti-staphylococcus aureus infection drugs, and belongs to the field of medicine. Background technique [0002] These three kinds of oroxin A, Oroxin B, Oroxylin A 7-O-glucuronide are three natural flavonoids isolated from the dry mature seeds of the Bignoniaceae plant Oroxinus, which are found in strawberries, grapes, apples It also exists in vegetables and fruits such as onions and onions. It has obvious anti-oxidation and anti-inflammatory properties, and has a certain regulatory effect on the immune system, but so far there have been no reports of fisetin being used to resist Staphylococcus aureus infection at home and abroad. [0003] Staphylococcus aureus is one of the most common opportunistic pathogens in clinical practice. More than 50% of adults carry Staphylococcus aureus in their bodies, and 10-20% of them are permanently colonized. In addition to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P31/04
Inventor 邓旭明牛效迪邱家章
Owner JILIN UNIV
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