The invention relates to a preparation method of a globin peptide iron chelate. The preparation method comprises main steps of preparing porcine blood corpuscle liquid, performing haemolysis, performing enzymolysis, performing centrifuging, performing ultrafiltration, performing chelating, and performing spray drying so as to obtain the globin peptide iron chelate. According to the method disclosed by the invention, porcine blood which is low in price and broad in source is used as a protein peptide source, inorganic iron salt is used as an iron source, iron elements and protein peptide are chelated, the technological process is simple, the cost is low, and the method is suitable for large-scale industrial production. The prepared product namely the globin peptide iron chelate is high in content of small and medium molecule peptide, and high in the content of chelating-state iron ions, and is a novel efficient iron supplement for feeds.