The invention relates to a
ginkgo episperm extract with antitumor and immunostimulating activity and a preparation method of an effective part thereof and belongs to the technical field of traditional Chinese
pharmaceutics.
Ginkgo episperm is decocted for 2-3 times in hot water, decocted fluid is filtered respectively, a combined
salt solution is added to filtrate after vacuum concentration, and after
water insoluble matters are filtered or removed by
centrifugation,
dialysis is carried out by
distilled water; after
dialysis internal fluid is concentrated,
precipitation is carried out by
ethanol, and the precipitate is dried, thus obtaining the
ginkgo episperm extract; the
ginkgo episperm extract is dissolved in water and is then pumped and filtered, continued filtrate flows through a
macroporous resin column, is eluted by
distilled water and is then eluted by the
ethanol when the
sugar test result of the eluent is negative, and then
ethanol eluent is collected, vacuum-concentrated and dried, thus obtaining the effective part of the ginkgo episperm extract. The content sums of
polysaccharide and
protein in the extract and the effective part are greater than 60 percent and 90 percent respectively, the water
solubility is good, the antitumor and immunostimulating activity is high,
solid formulation and fluid formulation can be prepared, and the preparation process is simple, convenient and feasible and is suitable for industrial
mass production.