Multi-crosslinking injectable hydrogel as well as preparation method and application thereof

A technology of multiple cross-linking and water injection, which is applied in the fields of pharmaceutical formula, medical science, prosthesis, etc., and can solve problems such as chronic inflammation and affecting the long-term success of implants

Active Publication Date: 2021-08-31
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]However, due to the rapid regeneration of soft tissues in the oral cavity, especially the mucosa, concerns about antibiotic resistance, and the relatively short (less than 60 years) history of implantation, people After the implant is placed, chronic inflammation may be caused by the inability to form an immediate seal around the implant, thereby affecting the long-term success of the implant. This issue has not attracted enough attention, and no corresponding intervention measures have been taken clinically

Method used

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  • Multi-crosslinking injectable hydrogel as well as preparation method and application thereof
  • Multi-crosslinking injectable hydrogel as well as preparation method and application thereof
  • Multi-crosslinking injectable hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] (1) Synthesis of CG

[0080] The synthesis scheme of CG is shown in the following formula:

[0081]

[0082] The specific steps are:

[0083] Weigh 1g chitosan (95% degree of deacetylation, MW=10W) and add 50ml 0.1mol L -1 Dilute hydrochloric acid, stir until completely dissolved, add NaOH dropwise to adjust the pH to 5.5, make up deionized water to make the CS concentration 1%.

[0084] Accurately weigh 3 g of gallic acid and dissolve in 25 mL of ethanol, weigh 3.39 g of EDC and 2.04 g of NHS and dissolve in 25 mL of ethanol. After the solution was completely dissolved, the mixed solution of GA solution, EDC and NHS was slowly poured dropwise into the stirring chitosan solution, stirred and reacted at room temperature for 24 hours, and kept at pH=5.0 during the reaction.

[0085] The reaction solution was dialyzed in a dialysis bag with a molecular weight cut-off of 3500 in DDW with pH=5 for 48 hours, and the dialyzed solution was collected and filtered to remove...

Embodiment 2

[0096] Specifically, the scheme in Example 1 is used to prepare hydrogels with different CG contents and different aldehyde-amine ratios, and to study the superiority and feasibility of the physical and chemical properties of the hydrogel for its application by characterizing various properties.

[0097] (1) Gelation time

[0098] Hydrogel Preparation Protocol Example 1.

[0099] Prepare CG-Gel mixed solution, mix MASA-CuSO 4 Add the mixed solution dropwise to the CG-Gel mixed solution under stirring at 500r / s. After mixing evenly, adjust the stirring speed to 10r / s. Observe that the rotation of the stirring bar is restricted or record the gelation time when stirring is stopped.

[0100] The result is as figure 1 As shown, the gelation time of the hydrogel is negatively correlated with the ratio of aldehyde groups to amino groups in the system. When the aldehyde-amine ratio is controlled to be 2:1 or above, the gelation time of the hydrogel can be controlled within 8 minutes...

Embodiment 3

[0136] (1) Antioxidant properties of antibacterial active hydrogels

[0137] The DPPH and ABTS free radical scavenging ability of the hydrogel in Example 1 was explored by DPPH and ABTS free radical scavenging experiments, and the influence of its thickness on the scavenging ability was explored to evaluate its antioxidant performance.

[0138] The result is as Figure 11 It shows that the free radical scavenging ability of the hydrogel is related to the thickness, and the thickness of the hydrogel gradually decreases in the same volume of the 96-well plate, 48-well plate, and 24-well plate, and the scavenging efficiency gradually increases. When it was prepared in 96-well plate and 48-well plate, it was found that the free radical scavenging efficiency increased with the increase of CG concentration. The efficiency does not change significantly with the increase of CG concentration, and the highest clearance rate can reach about 46.6%.

[0139] In the ABTS experiment, such ...

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Abstract

The invention discloses a preparation method of multi-crosslinking injectable hydrogel. The preparation method comprises the following steps of (1) preparing chitosan-gallic acid (CG); (2) preparing multiple aldehyde sodium alginate (MASA); and (3) preparing the multi-crosslinking injectable hydrogel. According to the method, gelatin, oxidized sodium alginate MASA, (chitosan-gallic acid) CG and copper ions are used as raw materials, and the process is simple and easy to control. The invention also discloses the multi-crosslinking injectable hydrogel prepared by adopting the preparation method of the multi-crosslinking injectable hydrogel. The invention further discloses the application of the multi-crosslinking injectable hydrogel in preparation of implant postoperative soft tissue sealing materials with the effects of being injectable, wet-bonding, antibacterial, anti-inflammatory, anti-oxidation, self-healing, degradable, suitable for mechanical properties and biocompatibility, promoting tissue regeneration and the like.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a preparation method of multiple cross-linked injectable hydrogel and its preparation method and application, especially a hydrogel with the functions of adhesive sealing, antibacterial, anti-inflammation, and promoting tissue regeneration. Multiple cross-linked injectable hydrogel and its preparation method and application. Background technique [0002] Tooth loss is so common that it is considered a chronic disability. Not only does it significantly reduce the quality of life related to oral health, both physically and psychologically, but it also increases the risk of many diseases such as cardiovascular and dementia. Oral implants are an ideal means of restoring missing teeth. They can restore and reconstruct tooth function and aesthetics, so they have become the choice of more and more patients. After the implant is implanted, the continuity of the soft ti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/26A61L27/54A61L27/58A61L27/56C08J3/075C08J3/24C08L5/08C08L5/04C08L89/00
CPCA61L27/52A61L27/26A61L27/54A61L27/58A61L27/56C08J3/075C08J3/246C08J2305/08C08J2305/04C08J2389/00A61L2400/06A61L2430/34A61L2300/104A61L2300/404A61L2300/41C08L5/08C08L5/04C08L89/00
Inventor 王琴梅洪育冰
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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