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Ginkgo episperm extract with antitumor and immunostimulating activity and preparation method of effective part thereof

A Ginkgo biloba exotesta and immune-promoting technology, which is applied in the direction of anti-tumor drugs, medical raw materials derived from Ginkgo biloba, drug combinations, etc., can solve the problem of unseparated effective parts, affecting the process of new drug development and application, and poor water solubility and other problems, to achieve the effect of simple and feasible preparation process, increased potency and good water solubility

Inactive Publication Date: 2010-12-15
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although the polysaccharides prepared by the previous process also have certain biological activities, due to their unsatisfactory water solubility, they can only be made into solid dosage forms, which limits the clinical application of some cancer diseases, and has not separated its effective Parts and further related research, thus affecting the process of new drug development and application

Method used

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  • Ginkgo episperm extract with antitumor and immunostimulating activity and preparation method of effective part thereof
  • Ginkgo episperm extract with antitumor and immunostimulating activity and preparation method of effective part thereof
  • Ginkgo episperm extract with antitumor and immunostimulating activity and preparation method of effective part thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Put 100kg of fresh testa in the cooking pot, add water to make the ratio of material to liquid about 1:8 (V / W). Keep the temperature in the cooking pot at 90-99°C for 1.5 hours, then pour out the decoction, repeat once, and decoct twice in total. After filtering the decoction liquid respectively, merge the filtrate, concentrate the filtrate under reduced pressure at 70°C to a density of 1.02, and then add a concentration of 25% (W / V) made of sodium chloride, sodium carbonate and magnesium sulfate. 8:1:1 combined salt solution, make the pH value of the concentrated filtrate 7.0, stir well and let it stand for 2 hours, filter again or centrifuge at 800 rpm for 5 minutes to remove water-insoluble matter, and remove water-insoluble The filtrate of the product was dialyzed with 50 times the volume of distilled water for 24 hours using a dialysis membrane with a cut-off value of 10,000. After concentrating the dialyzed liquid under reduced pressure to a density of 1.09, ethan...

Embodiment 2

[0046] Put 200kg of fresh testa in the cooking pot, add water to make the ratio of material to liquid about 1:6 (V / W). Keep the temperature in the cooking pot at 90-99°C for 1 hour, pour out the decocting liquid, repeat 2 times, and decoct 3 times in total. After filtering the decoction liquid respectively, merge the filtrate, concentrate the filtrate under reduced pressure at 70°C to a density of 1.04, and then add a concentration of 25% (W / V) made of sodium chloride, sodium carbonate and magnesium sulfate. 8:1:1 combined salt solution, so that the pH value of the concentrated filtrate is 7.2, after fully stirring, let it stand for 3 hours, filter again or centrifuge at 1000 rpm for 4 minutes to remove water-insoluble matter, and remove water-insoluble The filtrate of the product was dialyzed with 80 times the volume of distilled water for 12 hours using a dialysis membrane with a cut-off value of 8000. After the dialyzed liquid was concentrated under reduced pressure to a de...

Embodiment 3

[0048] Put 500kg of fresh testa in the cooking pot, add water to make the ratio of material to liquid about 1:12 (V / W). Keep the temperature in the cooking pot at 90-99°C for 2 hours, then pour out the decocting liquid, repeat 2 times, and decoct 3 times in total. After filtering the decoction liquid respectively, merge the filtrate, concentrate the filtrate under reduced pressure at 70°C to a density of 1.08, and then add a concentration of 25% (W / V) made of sodium chloride, sodium carbonate and magnesium sulfate. 8:1:1 combined salt solution, so that the pH value of the concentrated filtrate is 7.4, after fully stirring, let it stand for 4 hours, filter again or press 1200 rpm / centrifuge for 3 minutes to remove water-insoluble matter, and remove water-insoluble The filtrate of the product was dialyzed with 100 times the volume of distilled water for 18 hours using a dialysis membrane with a cut-off value of 7000. After the dialysis liquid was concentrated under reduced press...

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Abstract

The invention relates to a ginkgo episperm extract with antitumor and immunostimulating activity and a preparation method of an effective part thereof and belongs to the technical field of traditional Chinese pharmaceutics. Ginkgo episperm is decocted for 2-3 times in hot water, decocted fluid is filtered respectively, a combined salt solution is added to filtrate after vacuum concentration, and after water insoluble matters are filtered or removed by centrifugation, dialysis is carried out by distilled water; after dialysis internal fluid is concentrated, precipitation is carried out by ethanol, and the precipitate is dried, thus obtaining the ginkgo episperm extract; the ginkgo episperm extract is dissolved in water and is then pumped and filtered, continued filtrate flows through a macroporous resin column, is eluted by distilled water and is then eluted by the ethanol when the sugar test result of the eluent is negative, and then ethanol eluent is collected, vacuum-concentrated and dried, thus obtaining the effective part of the ginkgo episperm extract. The content sums of polysaccharide and protein in the extract and the effective part are greater than 60 percent and 90 percent respectively, the water solubility is good, the antitumor and immunostimulating activity is high, solid formulation and fluid formulation can be prepared, and the preparation process is simple, convenient and feasible and is suitable for industrial mass production.

Description

technical field [0001] The invention relates to a ginkgo biloba exotesta extract with anti-tumor and immune-promoting activities and a preparation method of the effective part of the extract, which belongs to the technical field of traditional Chinese medicine pharmacy. Background technique [0002] Ginkgo is a precious "living fossil" plant in the world, and China is the hometown of Ginkgo. The fruit of Ginkgo biloba, also known as ginkgo, is not only rich in nutrition, but also has been used as medicine for thousands of years. It is a world-renowned treasure of medicine and food. Ginkgo biloba contains compounds such as flavonoids and terpene lactones, and Schwabe Company in Germany took the lead in establishing a Ginkgo biloba extract (EGb) processing plant. After nearly 40 years of continuous research at home and abroad, Ginkgo biloba preparations have been widely used to treat cardiovascular and cerebrovascular diseases, which has laid a foundation for the comprehensiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/16A61P35/00A61P37/04A61K131/00
Inventor 许爱华陈华圣吴倩杨茜李俊
Owner YANGZHOU UNIV
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