Application of amentoflavone in research and development of medicines for treating pneumonia
A technology of biflavonoids and pneumonia, which is applied in the field of medicine and can solve the problems of treatment failure and the like
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[0008] Hemolysis test
[0009] The purified pneumolysin ply was serially diluted twice in a 96-well plate, and 10 7 Sheep red blood cells were incubated at 37°C for 10 min and then centrifuged. The highest dilution factor that lyses red blood cells is regarded as the hemolysis titre (Hemolysis titre)
[0010] The hemolytic titers of hemolysin ply without adding abiflavone and adding different concentrations of abiflavone are as follows (Table 1):
[0011] Table 1. Inhibitory effect of biflavonoids of Suicha chinensis on the hemolytic activity of pneumolysin
[0012]
[0013] Protective Test of Injured Human Lung Epithelial Cells (A549)
[0014] A549 cells were cultured in DEMED medium (containing 10% fetal bovine serum) at 37°C with 5% CO 2 Cultured to 1.5 x 10 4 Cells / well were plated in a 96-well cell culture plate, 100 μl per well. After the cells adhered to the wall, add 0.5 μl of purified pneumolysin protein, and add different concentrations of abiflavone, and pl...
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