127results about How to "Tolerated" patented technology

The invention discloses a soil heavy metal activating microbial inoculum and a preparation method and application thereof. Trichoderma viride, urease bacillus and bacillus mucilaginosus are high in tolerance to various heavy metals, and owing to a synergistic effect of bacterial strains, activation of the heavy metals in soil, promotion of plant absorption of the heavy metals and improvement of heavy metal pollution remediation efficiency of plants are realized. By adoption of organic wastewater as a raw material, heavy metal migration activity is further improved. Moreover, the soil heavy metal activating microbial inoculum is high in stability of application effects, the preparation method is simple and low in cost, and integration of heavy metal remediation, pest control and growth promotion for yield increase are realized.

The invention discloses bacillus amyloliquefaciens A18 with a preservation number of CCTCC NO: M 2018135 and a 16S rDNA sequence as shown in SEQ ID NO: 1. The invention also discloses a preparation method of the bacillus amyloliquefaciens, a liquid microbial inoculum and a solid microbial inoculum thereof, and application of the bacillus amyloliquefaciens in treating kitchen wastes. The bacillus amyloliquefaciens provided by the invention can rapidly degrade starch and protein with high contents in kitchen wastes and inhibit odor generation, can be used for rapid treatment of the kitchen wastes, and realizes rapid biological reduction and stabilization of the kitchen wastes.

The invention discloses bacillus subtilis JYM841 with antibacterial activity. The bacillus subtilis is preserved in a China center for type culture collection in January 10, 2014, and the preservation number is CCTCC NO: M 2014012. The bacillus subtilis JYM841 has a strong inhibitory effect on staphylococcus aureus and candida albicans, is high in antibacterial activity, wide in antibacterial spectrum, high in enzyme production, sensitive to bacterial antibiotic, drug-resistant to fungal antibiotic, high in heat-resisting ability and tolerant to acid and alkaline, has high antibacterial activity after treated at the temperature of 100 DEG C for 20min, and has tolerance to various enzymes such as trypsin, papain and protease K. An acid and alkali resistant pH (potential of hydrogen) value ranges from 6 to 10, and metabolite is stable, so that the bacillus subtilis can be applied to preparation of medicines for treating genital tract infection.

The invention discloses an application method of immobilized microorganisms in livestock breeding wastewater treatment. The technical flow comprises the following steps: (1) preparation of microbial fermentation broth; (2) preparation of the immobilized microorganisms; (3) induced culture of the immobilized microorganisms; (4) habituated culture; and (5) breeding wastewater treatment. According tothe invention, various microorganism symbiotic systems are constructed, and microorganisms can cooperate to effectively degrade organic substances, nitrogen, phosphorus and heavy metal pollutants inthe livestock breeding wastewater; a composite embedding material is adopted to immobilize the microorganisms, and a unique micro environment is formed to have a good shielding effect on outside invasion; resistance and tolerance of the immobilized microorganisms on the breeding wastewater are further improved through induced culture and habituated culture; and when the immobilized microorganismsare applied to a wastewater treatment system, stability is relatively high within a service period, and a good degradation effect is achieved on the livestock breeding wastewater, and therefore, a foundation is laid for practical application of the immobilized microorganisms in wastewater treatment.

The invention discloses a production method for lipid through synchronous mixed culture of microbes. The method comprises the following steps: (1) culturing facultative anaerobic bacteria seed liquid; (2) culturing autotrophic microalgae seed liquid; and (3) mixing the facultative anaerobic bacteria seed liquid and the autotrophic microalgae seed liquid and carrying out synchronous mixed culture in an illuminated bioreactor, wherein during synchronous mixed culture, an organic carbon source needed in growth and metabolism of the facultative anaerobic bacteria is supplemented. The method provided by the invention has the advantages of increased harvest yield of autotrophic microalgae, an improved utilization rate of an inorganic carbon source (CO2), increased content of microbial lipid, a simplified culture apparatus and the like, and exerts small influence on the fermentation process of the facultative anaerobic bacteria, thereby realizing synchronous culture of the facultative anaerobic bacteria and the autotrophic microalgae.

The invention discloses high-efficiency phosphate-solubilizing aspergillus japonicus with heavy metal tolerance. The high-efficiency phosphate-solubilizing aspergillus japonicus with the heavy metal tolerance is classified and named as aspergillus japonicus, and is collected in China General Microbiological Culture Collection Center; the collection number is CGMCC No.7700. The invention also discloses the tolerance of the high-efficiency phosphate-solubilizing aspergillus japonicus to heavy metals. The high-efficiency phosphate-solubilizing aspergillus japonicus has an extremely good dissolving effect on insoluble phosphates, such as tricalcium phosphate, magnesium phosphate and aluminum phosphate in the situation of liquid shake flask culture; the dissolving effects on the three types of insoluble inorganic phosphorus reach 100 percent. The aspergillus japonicus has certain tolerance to the heavy metals after being incubated in liquid culture solutions which contain the heavy metals such as Pb<2+>, Zn<2+>, Cr<2+>, Mn<2+>, Cu<2+>, As<5+> and Cd<2+>; particularly, the tolerated concentrations of the Pb<2+>, the Zn<2+>, the Cr<2+> and the Mn<2+> reach 2,000 mg/L respectively. Therefore, the high-efficiency phosphate-solubilizing aspergillus japonicus with heavy metal tolerance can provide excellent strain resources for the development of heavy metal-resistant high-efficiency phosphate-solubilizing bacterium agents.

The invention discloses a preparation method for a water-repellent oil-repellent superhydrophobic fabric surface. The preparation method comprises the following steps: (1) weighing a cotton fabric, and preparing a cotton-cellulose macromolecular initiating agent under the action of the initiating agent and a catalyst; (2) grafting monomeric GMA on the prepared cotton-cellulose macromolecular initiating agent, vacuumizing a reaction system and introducing nitrogen, so as to obtain cotton cellulose grafted by the GMA, soaking the cotton cellulose grafted by the GMA in an acid solution, and hydrolyzing epoxy groups to form a cellulose surface rich in hydroxyl groups; and (3) performing acylation reaction between the cotton cellulose grafted by the GMA on the cellulose surface rich in hydroxyl groups and a monomer with low surface energy to obtain the water-repellent oil-repellent superhydrophobic surface. The preparation method is mild in reaction condition, simple, and easy to operate, can be realized at room temperature, and can guarantee the good superhydrophobicity and oil resistance without influencing of the comfort and the mechanical property of the cotton fabric.

The invention discloses a production method for lipid through coupled culture of microbes. The method comprises the following steps: (1) culturing autotrophic microalgae seed liquid and carrying out enlarged culture until the OD value of biomass is 5.0 to 10.0, wherein the pH value of microalgae seed liquid having undergone enlarged culture is 9 to 12; (2) culturing facultative anaerobic bacteria seed liquid and carrying out fed-batch fermentation; and (3) during the process of fed-batch fermentation of facultative anaerobic bacteria, controlling the pH value in the fermentation process of the facultative anaerobic bacteria to be in a range of 6 to 9 in an illuminated bioreactor by using the prepared microalgae seed liquid having undergone enlarged culture in step (1) as an alkali neutralizing agent for bacterial fermentation and carrying out coupled culture of the facultative anaerobic bacteria and autotrophic microalgae. The method provided by the invention has the advantages of increased harvest yield of the autotrophic microalgae, an improved utilization rate of an inorganic carbon source, increased content of microbial lipid, a simplified culture apparatus and the like.

A gas separation membrane is formed from a copolyimide produced by copolymerization of monomers which include o-tolidine sulfone, at least one other hydrophilic diamine and 2,2-bis(3,4-dicarboxyphenyl) hexafluoropropane dianhydride. One or more additional aromatic dianhydrides can optionally be included. The novel membrane exhibits an excellent combination of high selectivity of gases to be separated from a gas mixture, high permeability and strong solvent resistance to hydrocarbon chemicals. The membrane is therefore useful in separating gases from gas mixtures in which vapor or liquid hydrocarbon contaminants are present, for example in the separation of carbon dioxide from methane and/or nitrogen in the purification of natural gas.

The invention discloses a surface modified lipidosome as well as a preparation method and application thereof. The lipidosome is prepared by mixing soya bean lecithin with cholesterol at mass ratio of (7.5-2.5) to 1 and is modified by lactobacillus acidophilus S-albumen. (1) The lactobacillus acidophilus S-albumen is adopted for modifying the lipidosome surface, so that the stability and storage of the lipidosome can be promoted. (2) The lipidosome has tolerance to gastrointestinal tract protease and the wrapped effective constituents can smoothly reach target tissues so as to effectively exert drug effects.

The invention discloses a fungal laccase gene Lac1. The nucleotide sequence of the fungal laccase gene Lac1 is shown in SEQ ID NO:1. The invention further discloses a construction method of a laccaserecombinant strain. The construction method comprises the following steps: connecting the fungal laccase gene Lac1 with an expression vector pPIC9K, performing linearization on the obtained recombinant plasmid pPIC9K/Lac1, converting pichia pastoris GS115, and constructing a pichia pastoris recombinant strain GS115/pPIC9K-Lac1; and performing induced fermentation on the pichia pastoris recombinantstrain, and screening the pichia pastoris recombinant strain with laccase activity as a laccase recombinant strain. The invention further provides a method for preparing recombinant laccase by usingthe laccase recombinant strain. The recombinant laccase is applied to dye decoloring.

The invention relates to the technical field of microbe application, particularly to an aspergillus niger JXJ A01 with potassium and phosphate dissolving and organophosphorus pesticide decomposing activity and application of the aspergillus niger JXJ A01 with the potassium and phosphate dissolving and organophosphorus pesticide decomposing activity to dissolution of insoluble potassium, organophosphorus and inorganic phosphorous, decomposition of organophosphorus pesticides and preparation of microbial fertilizers. The aspergillus niger JXJ A01 is stored in China General Microbiological Culture Collection Center with a preservation number of CGMCC No. 13775. The aspergillus niger JXJ A01 is high in dissolving effects on insoluble phosphorous and potassium ore powder and can convert the insoluble phosphorous and the potassium ore fertilizer into soluble phosphorous and potassium absorbable for plants and degrade the organophosphorus pesticide of omethoate into non-toxic soluble phosphate radicals to reduce pesticide concentration and increase the content of soluble phosphorous in soil. The aspergillus niger JXJ A01 with the potassium and phosphate dissolving and organophosphorus pesticide decomposing activity can be applied to increasing the content of soluble effective potassium and phosphorus in soil and repair pollution caused by the organophosphorus pesticides to soil.

The invention relates to a new Purpureocillium sp strain F1 and application thereof, in particular to the new Purpureocillium sp strain F1 separated from sludge subjected to sewage filtration in the sewage treatment plant of Yang Jiabao, Taiyuan, Shanxi Province, is preserved in China General Microbiological Culture Collection Center in December 17th, 2015, and has the classification name of Purpureocillium sp and the preservation number of CGMCC No.11813. The new Purpureocillium sp strain has wide application prospects on the aspect of heavy metal adsorption.

The invention provides a method for restoring soil polluted by heavy metal through a combination of magnetotactic bacteria and plants. The magnetotactic bacteria and the soil polluted by the heavy metal are mixed and irrigated, and magnetic fields are arranged on the soil polluted by the heavy metal to collect heavy metal polluted soil containing the magnetotactic bacteria, drip washing of the soil polluted by the heave metal is conducted and backfill is conducted; bamboo willows and crimson leafbeet are planted on the soil polluted by the heavy metal at the same time, and the crimson leafbeet is removed after 50-60 days; then crimson leafbeet is planted again, and the crimson leafbeet is removed after 50-60 days; the steps of planting and removal of the crimson leafbeet are repeated; and the bamboo willows and the crimson leafbeet are removed at the same time 1.5-2.5 years later, and the steps are executed circularly and repeatedly until the content of the heavy metal in the soil is up to the safety standard. Compared with the prior art, the magnetotactic bacteria are easy to collect, the growth cycle of the bamboo willows is short and the absorption efficiency of the heavy metal is high. The crimson leafbeet is easy to grow and has a hyperaccumulation ability of cadmium. The method is capable of restoring the soil which is polluted by various heavy metals at the same time, and free of secondary pollution to the soil.

The invention relates to a method for improving the transformation efficiency of soybean. A method for selecting transformed plant cells comprises the following steps: transforming the plant cells by using a recombinant carrier of a gene containing a target gene and a gene for encoding sulfonylurea herbicide hydrolase; carrying out screening culture on the transformed plant cells by externally applying an ALS (Acetolactate Synthetase) inhibitor, and using the gene for encoding the sulfonylurea herbicide hydrolase as a selected marker; selecting the plant cells which are not killed and/or not inhibited. According to the method disclosed by the invention, a method of adding a selective agent into an enrichment culture medium and a differential culture medium by an external application mode (in particular to dropping) in the plant transforming process is put forward for the first time, and effective concentration screening range of the selective agent is optimized, and the proportion and the transformation efficiency of positive plants obtained by an offspring are remarkably improved; meanwhile, transgenic plants obtained by transformation using the sulfonylurea herbicide hydrolase as the selected marker have the advantages of high commercial value, good resistance and genetic stability.

The invention discloses magnetic nano-chitosan microspheres and a preparation method thereof, and belongs to the technical field of preparation of magnetic nanomaterials. The preparation method comprises the following steps: (1) adding NaOH into a ferric chloride solution for adjusting to alkaline, filtering initial precipitate, mixing the initial precipitate with a dispersion solution, and removing ethanol to obtain a dispersion phase of the initial precipitate; placing the dispersion phase of the initial precipitate into a high-pressure reaction kettle for reacting to obtain a dispersion phase of secondary precipitate, wherein the secondary precipitate is magnetic nanoparticles; (2) adding the magnetic nanoparticles into a chitosan solution, then adding an emulsifier and a fixative, separating to obtain a ternary precipitate, drying the ternary precipitate to obtain the magnetic nano-chitosan microspheres. The invention intends to provide a magnetic nanocarrier which has the characteristics of high biological property, carrying of various active groups, large specific surface area, porosity, narrow particle size distribution and the like. The magnetic nano-chitosan microspheres can perform rapid movement and separation under the action of an external magnetic field, so that the microspheres have the advantages of easiness in recovery, reproducibility and the like.

The invention discloses a remediation method for improving the remediation effect of heavy metal contaminated soil. The method comprises the following steps: (1), stripping soil with the thickness of10-50cm from the surface layer of heavy metal contaminated soil, stacking the soil into a remediation pool, and inoculating magnetotactic bacteria; (2), after culturing for 15-25 days, enabling the soil in the remediation pool to be completely soaked in water, setting a magnetic field, and keeping the state for 20-25 days; (3), draining water in the remediation pool, and then leaching the soil inthe pool with clear water; (4), spraying liquid humic acid into the soil in the remediation pool; (5), adding molasses into the heavy metal contaminated soil with the surface soil stripped, spraying liquid humic acid after ploughing and standing, backfilling the soil in the (4), and planting plants capable of enriching heavy metal; and (6), regularly harvesting, removing and reseeding the plants capable of enriching the heavy metals in the (5), and regularly detecting the content of each heavy metal in the heavy metal contaminated soil until the content of the heavy metals in the heavy metal contaminated soil reaches the safety standard.

The invention belongs to the technical field of microalgae breeding, and particularly relates to a microalgae breeding method with high CO2 tolerance and fixed rate. The method specifically comprises the steps of sample collection and preculture, simulated flue gas habituated culture, flat separation, relatively pure population collection, pure population acquisition, purebred saving, cultural character testing, high-intensity habituated culture, gene mutation breeding, passage stability testing, gene cloning and sequencing, algal strain carbon content testing, target strain cultural character testing, and optimum target strain determination. Cultivated strain has the CO2 tolerance under the maximal condition. The whole method is scientific in principle, the adopted steps are clear, operation is easy, and conditions are controllable.