Bacillus amyloliquefaciens, microbial inoculum as well as application of bacillus amyloliquefaciens in treating kitchen wastes

Abstract

The invention discloses bacillus amyloliquefaciens A18 with a preservation number of CCTCC NO: M 2018135 and a 16S rDNA sequence as shown in SEQ ID NO: 1. The invention also discloses a preparation method of the bacillus amyloliquefaciens, a liquid microbial inoculum and a solid microbial inoculum thereof, and application of the bacillus amyloliquefaciens in treating kitchen wastes. The bacillus amyloliquefaciens provided by the invention can rapidly degrade starch and protein with high contents in kitchen wastes and inhibit odor generation, can be used for rapid treatment of the kitchen wastes, and realizes rapid biological reduction and stabilization of the kitchen wastes.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to bacillus amyloliquefaciens and bacterial agents and applications. Background technique [0002] In recent years, with the improvement of people's living standards and the continuous development of the catering industry, the amount of kitchen waste is also increasing year by year, accounting for nearly half of urban domestic waste. If it is not properly disposed of, kitchen waste will pollute the environment and breed mosquitoes. Flies, causing the spread of disease, so that endanger human health. Food waste mainly comes from leftover food, which has a water content of 70%, so it is not suitable to be disposed of by incineration, and sanitary landfill will occupy a large amount of land resources. The enlargement becomes increasingly severe. Biological treatment has become the first choice for food waste treatment, but food waste usually contains high salt, high protein, h...

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Problems solved by technology

There are two main directions for biological treatment, one is the energy conversion of biological treatment; for example, Jiangsu Clean Environment Technology Co., Ltd. (Zhang Longsheng et al., 2009) researched the recovery of oily components in kitchen waste into biodiesel, and the Sunkeehan research group of the Korean Academy of Sciences (2004) turned waste into ammonia gas, and Xiao Benyi, a researcher at the Chinese Academy of Sciences (2004), invented a two-phase anaerobic fermentation technology, which improved the recovery of organic biomass energy from food waste. There are also recycling food waste fermentation abroad Reports on the production of hyaluronic acid and sodium dioic acid (Doyle et al, 1982). These research works have provided many useful explorations for the energy conversion of food waste, but there are still many bottlenecks in the application of new bio-energy , so most of these studies are still at the level of scientific research

The other direction is bio-fermentation resource treatment. At present, the composting application of food waste is widely recognized in production. The rich nutrients of food waste can meet the nutritional needs of microbial growth, which is very suitable for composting raw materials. , composting technology appeared very early, but now, there are still many problems to be studied, such as how to breed and match efficient and fast degrading bacteria, if the bacteria are preserved, so that they can be commercialized, and how to remove food waste during the degradation process. pollution, how to adapt to the multiple requirements of the society for the scale of food waste composting, etc.

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