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Bacillus amyloliquefaciens, microbial inoculum as well as application of bacillus amyloliquefaciens in treating kitchen wastes

A technology for decomposing starch spores and kitchen waste, which is applied in the field of microorganisms, can solve problems such as retention, and achieve the effects of odor release, high weight loss rate, and high activity

Inactive Publication Date: 2018-11-16
SHENZHEN MICRON BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are two main directions for biological treatment, one is the energy conversion of biological treatment; for example, Jiangsu Clean Environment Technology Co., Ltd. (Zhang Longsheng et al., 2009) researched the recovery of oily components in kitchen waste into biodiesel, and the Sunkeehan research group of the Korean Academy of Sciences (2004) turned waste into ammonia gas, and Xiao Benyi, a researcher at the Chinese Academy of Sciences (2004), invented a two-phase anaerobic fermentation technology, which improved the recovery of organic biomass energy from food waste. There are also recycling food waste fermentation abroad Reports on the production of hyaluronic acid and sodium dioic acid (Doyle et al, 1982). These research works have provided many useful explorations for the energy conversion of food waste, but there are still many bottlenecks in the application of new bio-energy , so most of these studies are still at the level of scientific research
The other direction is bio-fermentation resource treatment. At present, the composting application of food waste is widely recognized in production. The rich nutrients of food waste can meet the nutritional needs of microbial growth, which is very suitable for composting raw materials. , composting technology appeared very early, but now, there are still many problems to be studied, such as how to breed and match efficient and fast degrading bacteria, if the bacteria are preserved, so that they can be commercialized, and how to remove food waste during the degradation process. pollution, how to adapt to the multiple requirements of the society for the scale of food waste composting, etc.

Method used

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  • Bacillus amyloliquefaciens, microbial inoculum as well as application of bacillus amyloliquefaciens in treating kitchen wastes
  • Bacillus amyloliquefaciens, microbial inoculum as well as application of bacillus amyloliquefaciens in treating kitchen wastes
  • Bacillus amyloliquefaciens, microbial inoculum as well as application of bacillus amyloliquefaciens in treating kitchen wastes

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preparation example Construction

[0033] The third object of the present invention is to provide a preparation method of Bacillus amyloliquefaciens liquid bacterial agent, comprising

[0034] Fermentation step: Streak the frozen-preserved Bacillus amyloliquefaciens A18 strain on the plate with plate activation medium, and cultivate overnight at 35-40°C;

[0035] Preparation steps of fermented liquid seeds: pick well-growth plate colonies in the seed culture medium, cultivate overnight at 35-40°C, 180r / min, to obtain liquid seeds;

[0036] The steps of preparing the liquid bacterial agent: the inoculum amount of the above-mentioned liquid seed fermentation medium is 5-10%, placed at 30-37° C., 150-220 r / min, and fermented for 12-24 hours to obtain the liquid bacterial agent of Bacillus amyloliquefaciens.

[0037] As a further solution, the plate activation medium of the present invention comprises beef extract 3g / L, peptone 10g / L, NaCl 15g / L, and agar 20g / L; the pH of the plate activation medium is 7.2-7.4.

...

Embodiment 1

[0047] Embodiment 1: Isolation and screening of Bacillus amyloliquefaciens

[0048] The separation process of Bacillus amyloliquefaciens of the present invention: the test samples are collected from the buckets of the kitchen garbage truck in the canteen of Nanjing University. Scrape the remaining kitchen waste from the bucket, put it in a plastic sealed bag, and store it at room temperature. Because the sample is difficult to dissolve in water and cannot be completely separated in water, the number of degrading bacteria in the obtained dilution is small, so the method of direct solid separation is adopted in this paper. Take a sample with a diameter of about 1 cm from the collected food waste, connect it to the center of the starch and protein medium, place it in an incubator at 37°C, and observe the growth of the colony. If there is mold in the medium, it will cause pollution when the bacteria are separated by streaking, so nystatin is added to the medium used to inhibit th...

Embodiment 2

[0053] Embodiment 2: the preparation of bacillus amyloliquefaciens liquid bacterial agent

[0054] The bacterial agent contains Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) A18 at a concentration of 10 9 CFU / mL-10 10 CFU / mL; its preparation method, including

[0055] Fermentation step: Streak the frozen-preserved Bacillus amyloliquefaciens A18 strain on the plate with plate activation medium, and cultivate overnight at 35-40°C;

[0056] Preparation steps of fermented liquid seeds: pick well-growth plate colonies in the seed culture medium, cultivate overnight at 35-40°C, 180r / min, to obtain liquid seeds;

[0057] The steps of preparing the liquid bacterial agent: the inoculum amount of the above-mentioned liquid seed fermentation medium is 5-10%, placed at 30-37° C., 150-220 r / min, and fermented for 12-24 hours to obtain the liquid bacterial agent of Bacillus amyloliquefaciens.

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Abstract

The invention discloses bacillus amyloliquefaciens A18 with a preservation number of CCTCC NO: M 2018135 and a 16S rDNA sequence as shown in SEQ ID NO: 1. The invention also discloses a preparation method of the bacillus amyloliquefaciens, a liquid microbial inoculum and a solid microbial inoculum thereof, and application of the bacillus amyloliquefaciens in treating kitchen wastes. The bacillus amyloliquefaciens provided by the invention can rapidly degrade starch and protein with high contents in kitchen wastes and inhibit odor generation, can be used for rapid treatment of the kitchen wastes, and realizes rapid biological reduction and stabilization of the kitchen wastes.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to bacillus amyloliquefaciens and bacterial agents and applications. Background technique [0002] In recent years, with the improvement of people's living standards and the continuous development of the catering industry, the amount of kitchen waste is also increasing year by year, accounting for nearly half of urban domestic waste. If it is not properly disposed of, kitchen waste will pollute the environment and breed mosquitoes. Flies, causing the spread of disease, so that endanger human health. Food waste mainly comes from leftover food, which has a water content of 70%, so it is not suitable to be disposed of by incineration, and sanitary landfill will occupy a large amount of land resources. The enlargement becomes increasingly severe. Biological treatment has become the first choice for food waste treatment, but food waste usually contains high salt, high protein, h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09B3/00C12R1/07
CPCB09B3/00C12N1/20C12N1/205C12R2001/07
Inventor 邓涛陈学明
Owner SHENZHEN MICRON BIOTECH CO LTD
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