Burkholderia kururiensis strain and application thereof

A technique for Burkholderia spp., strains

Active Publication Date: 2015-09-16
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Burkholderia can produce a variety of metabolites with antibacterial activity, such as siderophore, phenazine, nipyrrolicin, phenylpyrrole, monoterpene alkaloid, Cepaciamide A, CepacidineA, Cepacin A, etc., which have been used in Biological control, decomposing toxic substances and other fields, but no Burkholderia bacteria with the functions of nitrogen fixation, phosphorus dissolution and potassium dissolution have been found

Method used

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  • Burkholderia kururiensis strain and application thereof
  • Burkholderia kururiensis strain and application thereof
  • Burkholderia kururiensis strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 The acquisition of Burkholderia yy01

[0029] Since medicinal wild rice has been in the wild state for a long time, it has withstood various disasters and natural selection of the environment, so that it has the characteristics of resistance to diseases and insect pests, drought resistance, and poor soil resistance. The inventor used Guangxi medicinal wild rice as a material, and separated and screened it Out of Burkholderia Kururi yy01.

[0030] Separation and purification: Take Guangxi medicinal wild rice and wash it with distilled water, cut off the roots, stems and leaves with a length of 3~5cm, put them in 3 sterilized petri dishes, soak them in 70% ethanol for 5min, and then wash them with 0.1% ethanol HgCl 2 Soak for 3 minutes, wash with sterile water 7 times, 8 minutes each time, and apply the last washing solution on the LB solid medium to test whether the surface disinfection is thorough. Cut the thoroughly sterilized roots, stems and leaves with...

Embodiment 2

[0034] Example 2 Identification of Burkholderia yy01

[0035]Strain yy01 is short rod-shaped, the optimum growth temperature is 37 ℃, the optimum growth pH is 6~8, the NaCl resistance concentration reaches 3.0%, and it secretes catalase and urease. The bacterial strain grows faster on the LB agar plate. After growing for 36 hours at 37° C., the bacterial colony is round and raised, beige and opaque, and the diameter of the bacterial colony is 3-6 mm. Through the determination of physiological and biochemical characteristics, it shows that the bacterium is facultative anaerobic, methyl red reaction is positive, V.P reaction is negative, catalase is positive, can not liquefy gelatin and hydrolyzed starch; indole reaction is positive, and can secrete auxin IAA; has the ability to produce ammonia and can secrete urease. The pH growth range of the strain is small, it can tolerate pH6~8, and the NaCl concentration can reach 3%. Tolerance; and 5μg / mL of laramycin, gentamicin, str...

Embodiment 3

[0038] Example 3 Phosphorus-dissolving and potassium-dissolving and nitrogen-fixing performance experiments of Burkholderia yy01

[0039] The strain yy01 was used to measure the ability of dissolving phosphorus and potassium. The strains were inoculated on PKO and potassium selective culture plates, and the qualitative test of dissolving phosphorus and potassium was carried out by the transparent circle method. The results are shown in figure 2 , the strain yy01 can form a very obvious transparent circle, the phosphorus-dissolving ratio is 2.92, and the potassium-dissolving ratio is 4.13. Then with Klebsiella variicola As a reference strain, the quantitative test of dissolving phosphorus and potassium was carried out by the molybdenum-antimony anti-colorimetric method and the sodium tetraborate method. The results are shown in Table 1. It shows that the amount of inorganic phosphorus dissolved by strain yy01 reaches 116.28 mg / L, which is the reference strain Klebsiella ...

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Abstract

The invention belongs to the technical field of microorganisms, and specifically discloses a Burkholderia kururiensis strain and application thereof. The strain is Burkholderia kururiensis yy01, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on March 17, 2015, and has preservation No. CGMCC No. 10631. The strain yy01 has both efficient releasing of phosphorus and potassium and high nitrogenase activity, increases the effectiveness of insoluble phosphorous and potassium in soil and convert free nitrogen in the air into ammonia for absorption and utilization by crops, and has significant growth-promoting effect on rice, tobacco and other crops.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Burkholderia kururi and its application. Background technique [0002] Nitrogen, phosphorus, and potassium are the three major nutrients necessary for plant growth, but the contents of nitrogen, phosphorus, and potassium in most soils in my country are relatively low. Phosphorus in the soil mainly exists in the form of phosphate, while potassium mainly exists in the form of minerals in potassium feldspar and mica, which are difficult for plants to absorb and utilize. For a long time, in order to ensure the high yield of agricultural products, people have used chemical fertilizers and pesticides in large quantities, which not only caused the destruction of soil structure and environmental pollution, but also was not conducive to the sustainable development of agriculture. It is one of the research directions in the field of microbial technology to convert free ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P21/00B09C1/10C12R1/01
CPCA01N63/10C05F11/08C12N1/205C12R2001/01
Inventor 谭志远阳洁彭桂香
Owner SOUTH CHINA AGRI UNIV
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