48results about How to "Increase nitrogenase activity" patented technology

The invention relates to a compound inoculant for improvement of saline-alkali soil and particularly provides a novel compound inoculant for improvement of saline-alkali soil, and preparation and applications of same. Bacillus amyloliquefaciens, bacillus megatherium, bacillus mucilaginosus and paenibacillus azotofixans are fermented respectively to obtain fermentation liquids, which are then mixed with a carrier respectively according to certain volume-weight ratio (V / kg); the mixtures are dried at low temperature and finally are compounded according to ratio. The compound inoculant is a powder agent, wherein the microorganisms therein are functional bacteria, and the inoculant is high in living bacteria number and especially contains high-activity metabolic products, so that the compound inoculant is suitable for being mixed with a base fertilizer, especially high-quality farmyard manure, for use. When being applied in soil, high environment adaption capability and high reproduction speed are achieved. The compound inoculant can increase beneficial microbial flora in rhizosphere and rapidly decompose organic substances for absorption and utilization by plants, can reduce the alkalinity of soil, improve fertility of soil and improve the water permeability and retention and breathability properties of the soil, and can accelerate growth of root system and prevent spreading of soil-borne diseases, thereby maintaining ecological balance, reducing cost and improving the saline-alkali soil.

The invention discloses a brevibacterium halotolerans with relatively high azotase activity and application thereof in ecological restoration. The brevibacterium halotolerans GDSD21 is collected in the China General Microbiological Culture Collection Center with the collection number of CGMCC No.12402. Nitrogen-fixing bacteria are separated from rhizosphere soils of field-crop roots, further strains with relatively high azotase activity are screened, and finally the efficient nitrogen-fixing bacterium brevibacterium halotolerans GDSD21 is screened. The azotase activity of the brevibacterium halotolerans GDSD21 is obviously higher than that of common production strain azotobacter chroococcum ACCC11103 for microbial fertilizers, and the brevibacterium halotolerans GDSD21 has extensive application prospects in soil improvement, increasing of the soil fertility, ecological restoration of a virgin land, and production of industrialized seedling-rearing inoculation agents, nitrogen-fixing microbial agents and bio-organic fertilizers for vegetables.

The invention provides a microbial soil improving agent for restoring petroleum polluted soil. A product is prepared from a degradation bacterial agent, an additive and a biosurfactant, wherein the degradation bacterial agent is prepared from pseudomonas, bacillus, Acinetobacter, microzyme, phosphate-solubilizing bacteria, azotobacterial, nitrobacteria and photosynthetic bacteria; the additive isprepared from the following ingredients in parts by weight: 20 to 35 parts of wormcast, 80 to 120 parts of bentonite, 1 to 10 parts of chitosan, 10 to 20 parts of kaolin, 5 to 9 parts of borax, 25 to35 parts of crop stalks, 10 to 15 parts of rice hull powder, 10 to 15 parts of dry seaweed, 1 to 5 parts of organic acid, 5 to 10 parts of vermiculite, 10 to 20 parts of shell powder and 20 to 25 parts of compound amino acid. The microbial soil improving agent for restoring petroleum polluted soil has the advantages that the soil fertility is improved; the petroleum in the soil can be degraded; the goal of governing the petroleum polluted soil is achieved.

The invention discloses a RY2 strain of a root nodule nitrogen-fixing bacterial strain system and application thereof. The strain (Bradyrhizobium yuanmingense RY2) is preserved in the China Center for Type Culture Collection (CCTCC) on 13th November in 2009. The preservation number is CCTCC NO:M 209266. The RY2 strain of the root module nitrogen-fixing bacterial strain system can exclusively greatly improve the nitrogenase activity of TPRC2, so that the nitrogenase activity of the TPRC2 is over three times higher than that of TPRC5 and can achieve higher nitrogenase activity by using a few root nodules. The yield of stylosanthes guianensis can be improved by over 30 to 40 percent by inoculating the root nodule bacteria, and the root nodule bacteria can be suitable for intercropping of stylosanthes guianensis TPRC2 and provides a large number of nitrogen for the stylosanthes guianensis, crops and soil. Meanwhile, the RY2 has strong salt tolerance, can grow in the environment that the NaCl concentration is higher than 0.25mol / L, can be planted in the salinized soil and has better effects of improving the yield of the stylosanthes guianensis and utilizing and improving the salinized soil.

The invention discloses a cysteine protease gene for regulating and controlling root nodule senescence, and a preparation method and application thereof. According to the preparation method disclosed by the invention, a Japan lotaustralin genomic sequence library is compared by utilizing the known cysteine protease gene of astragalus smicus; after the Japan lotaustralin genomic sequence library is analyzed and compared on the internet in a detailed manner, a primer having strong specificity is designed to carry out gene cloning by taking initiation codon as the starting point and termination codon as the finishing point; in addition, due to sequencing analysis and comparing analysis, a cloned fragment proves that the cysteine protease gene for regulating and controlling root nodule senescence is successfully cloned from the lotaustralin genomic sequence and cDNA (complementary deoxyribonucleic acid). The invention discloses application of the cysteine protease gene on leguminous plants; the expression mode of a gene promoter and the functions of the gene in the root nodule senescence process are researched by utilizing a histochemical localization technology. Compared with contrast plants, the results show that the root nodule of an RNAi (ribonucleic acid interference) plant is enlarged; the nitrogen fixation enzyme activity is increased; senescence of the root nodule is delayed; the effective nitrogen fixation time of the root nodule is prolonged; growth of the plant is more vigorous; the cysteine protease gene disclosed by the invention has application prospect on leguminous crops.

The invention relates to the technical field of sugarcane planting, and particularly discloses a planting method for promoting nitrogen fixation of sugarcane. The method comprises the following stepsof detoxifying single-bud seed stems of the sugarcane; carrying out sand culture on the single-bud seed stems of the sugarcane, and carrying out nitrogen fixation bacterium inoculation treatment on sugarcane seedlings when each sugarcane seedling grows out 3-4 leaves; scientifically and cooperatively applying nitrogen-phosphorus-potassium base fertilizer, nitrogen fixation microbial fertilizer andorganic fertilizer in a soil preparation trench for providing nutrition required by sugarcane growth, and planting the sugarcane seedlings subjected to inoculation treatment; and after the sugarcaneseedlings grow for 2-3 months, spraying a nitrogen fixation synergist solution to the leaf surfaces of the sugarcane. According to the planting method, the nitrogen fixation capacity of the sugarcaneis improved, the application frequency and the application amount of nitrogen fertilizer are reduced, the yield and quality of the sugarcane are improved, and the economic benefits of the sugarcane are improved.

The invention belongs to the technical field of microorganisms, and specifically discloses a Burkholderia kururiensis strain and application thereof. The strain is Burkholderia kururiensis yy01, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on March 17, 2015, and has preservation No. CGMCC No. 10631. The strain yy01 has both efficient releasing of phosphorus and potassium and high nitrogenase activity, increases the effectiveness of insoluble phosphorous and potassium in soil and convert free nitrogen in the air into ammonia for absorption and utilization by crops, and has significant growth-promoting effect on rice, tobacco and other crops.

The invention discloses a biological nitrogen fixation synergist. The biological nitrogen fixation synergist is prepared from in percentage by weight: 0.05-5% of charcoal, 0.01-5% of graphene sol, 0.01-1% of iron nutrient, 0.01-1% of molybdenum nutrient, 0.00001-0.0005% of brassinolide active substance, 0.1-1% of surfactant and the balance stopping. The biological nitrogen fixation synergist provides rich uncombined carbon, can promote colonization of nitrogen-fixing microorganisms, improves the dinitrogenase activity, promotes nitrogen absorption and utilization of plants, and improves the yield. The invention further provides a use method of the biological nitrogen fixation synergist.

The invention relates to the technical field of nitrogen-fixing microorganism bacterium agents and in particular relates to a nitrogen-fixing microorganism G96 as well as a bacterium agent preparation method and application thereof. The nitrogen-fixing microorganism G96 is preserved in China Center for Type Culture Collection, with the preservation number of CCTCCM 2015752. The bacterium agent preparation method of the nitrogen-fixing microorganism comprises the following steps: (1) separating and screening; (2) purifying and preserving; (3) carrying out solid fermentation culture; (4) preparing the bacterium agent to obtain a bacillus megaterium G96 bacterium agent. The nitrogen-fixing microorganism G96 not only has relatively high nitrogenase activity, but also has a very high IAA (Indole Acetic Acid) secretion capability; the nitrogenase activity is 1000nmol / (mL h) to 1100nmol / (Ml h); the secretion amount of the IAA is 300mg / L to 400mg / L in a growth and anabolism process of the nitrogen-fixing microorganism.

The invention discloses a Sclerotinia sclerotiorum-antagonizing nitrogen-fixing spore bacterium and its application. The nitrogen-fixing spore bacterium is Bacillus sp. GDSD212, which is preserved in General microbiological center of China Committee for Culture Collection of Microorganisms with a preservation number of CGMCC No.5035. The Bacillus sp. GDSD212 CGMCC No.5035 provided in the invention has high nitrogen-fixing activity, can effectively antagonize Sclerotinia sclerotiorum, and has a broad application prospect in production of nitrogen-fixing microorganism bacterial agents and bio-organic fertilizers.

The invention relates to a construction method of genetic engineering strains for improving quantity of soybean nodules. The construction method aims at solving the problems that when the conventional rhizobium japonicum genetic engineering strains are inoculated to soybean seedlings, the root nodule quantity of soybean plants and the yield increase of soybeans are small compared with those of the original starting strains. The construction method comprises the following steps of: performing activation and culture expansion on sinorhizobium fredii to extract genomic DNA (deoxyribonucleic acid); performing PCR (Polymerase Chain Reaction) amplification by virtue of a homologous sequence cloning method to obtain target gene dct A; performing sequencing identification on the target gene; constructing a prokaryotes expression vector pTR-Plac-dct A; and converting the prokaryotes expression vector into the sinorhizobium fredii to obtain the gene engineering strains. The quantity of the root nodules on the soybean plant with the transformed gene engineering strains is increased by 53.73%, and the yield of the soybeans is increased by 62.02%.

The invention belongs to the technical field of microorganisms, and relates to a mutant strain as well as a construction method and application thereof. The pseudomonas stutzeri mutant strain is pseudomonas stutzeri DSM4166-TP (nifR), is preserved in China General Microbiological Culture Collection Center on December 20, 2019, and has a preservation number of CGMCC No.19081. The pseudomonas stutzeri mutant strain has a preservation number of CGMCC No.19081. The pseudomonas stutzeri mutant strain has the beneficial effects that compared with the existing wild type DSM4166 strain, the pseudomonas stutzeri mutant strain has the advantages that the nitrogenase synthesis gene expression level is greatly improved, and the activity of the produced nitrogenase is also greatly higher than that of the wild type DSM4166 strain. The pseudomonas stutzeri mutant strain disclosed by the invention can be industrially produced on a large scale and has a wide commercial value.

The invention discloses a wheat rhizosphere azotobacter for antagonism of various pathogenic fungi and application of the wheat rhizosphere azotobacter. The wheat rhizosphere azotobacter is Paenibacillus sp. GD641, and has the collection number of CGMCC No.5042 in China General Microbiological Culture Collection Center. The Paenibacillus sp. GD641 with the collection number of CGMCC No.5042 has effective antagonism on Gibberella zeae, Sclerotinia sclerotiorum and Verticillium dahlia of crops, and has wide application prospect in microbial azotobacter and biological organic fertilizer production.

The invention relates to the technical field of functional microorganism screening and application, and specifically provides a new methylotrophic bacillus ( Bacillus methylotrophicus ), and its application in agricultural production is provided. The methylotrophic bacillus is screened from the soil in the Minsk area of ​​Belarus, and the preservation number is CCCTCC NO: M2019439. It has a significant inhibitory effect on various pathogenic bacteria, can effectively prevent and control common crop diseases, and increase crop yield, with broad application prospects.

The invention relates to the field of microorganisms, and particularly discloses an ammonium-resistant nitrogen-fixing microorganism of which the gene glnA is lost and a constructing method and application thereof. The ammonium-resistant nitrogen-fixing microorganism of which the gene glnA is lost can be subjected to biological nitrogen fixation under both an ammonium-free condition and an ammonium condition, the activity of dinitrogenase of paenibacillus microorganisms under a high-ammonium condition is improved, the inhibiting effect of biological nitrogen fixing under a high-ammonium condition is broken through, and the ammonium-resistant nitrogen-fixing microorganism has a wide application prospect in agricultural production.

The invention relates to a preparation method of fast-growing frankia powder. The method comprises the following steps: S1, breeding a frankia strain; S2, treating the pure frankia strain, obtained instep S1, by using a gene mutagenesis method to obtain a fast-growing frankia strain; S3, inoculating the fast-growing frankia strain, obtained in the step S2, into sterilized fermented raw materialsin a fermentation tank to form a bacterial culture material, and carrying out fermenting cultivation on the bacterial culture material to form hyphae; S4, further differentiating the hyphae, obtainedin the step S3, into spores, and eluting with sterile water to obtain bacterial liquid; adding sterile activated carbon into the bacterial liquid for adsorbing the spores, and separating the spore-containing activated carbon from the liquid after the spore adsorption is completed so as to obtain thallus taking the activated carbon as a carrier; S5, enabling the thallus, obtained in the step S4, tobe subjected to freeze-drying treatment by using a vacuum freeze-drying method so as to obtain the bacterial powder. The preparation method, provided by the invention, of the fast-growing frankia powder is good in inoculation effect, rapid in growth speed and suitable for large-scale industrial production.

The invention is related to a symbiotic system comprising a plant of the Leguminosae family, and a bacterium of the rhizobium family, wherein in said bacterium a gene coding for a flavohemoglobin protein is over expressed. The present invention is also related to a method for delaying the senescence of symbiotic nodules, comprising inoculating a plant of the Leguminosae family with a bacterium of the rhizobium family, wherein said rhizobium overexpresses a gene coding for a flavohemoglobin protein. A method for cultivating a plant of the Leguminosae family is also disclosed.

The invention relates to the technical field of microorganisms, and particularly relates to Sphingobium abikonense HP10 and application thereof. The Sphingobium abikonense HP10 is preserved in the China General Microbiological Culture Collection Center on January 13, 2021, and the preservation number is CGMCC No. 21618. The strain has high nitrogenase activity, can degrade spirodiclofen, can inhibit bacteria and secrete indoleacetic acid. The Sphingobium abikonense HP10 can be used for microbial nitrogen fertilizer, can also serve as a soil residual pesticide degrading agent, can promote plant growth and has great significance in planting hybrid paper mulberry in northwest regions.

The invention relates to the technical field of sugarcane planting, and particularly discloses a sugarcane weight-losing and efficiency-improving planting method which comprises the following steps: (1) soil preparation; (2) seed stem treatment; (3) sugarcane planting; (4) field management; (5) harvesting. According to the planting method, a proper amount of nitrogen fertilizer is intensively applied in the early growth stage of sugarcane, phosphorus-potassium fertilizer and microelement fertilizer are cooperatively applied, the PH value of soil and water supply are adjusted, meanwhile, nitrogen-fixing bacteria liquid is adopted for soaking seeds, compound microorganism fertilizer is adopted as base fertilizer, early-stage growth of sugarcane can be accelerated, sugarcane plants can form strong root systems and robust overground stem leaves, and the yield of sugarcane is increased. Fertilization is not needed in the large growth period (elongation period) after the sugarcane is closed, biological nitrogen fixation of the sugarcane is promoted, nitrogen nutrition needed by growth of the sugarcane is provided, and compared with a traditional'three-attack and one-supplement 'fertilization method, implementation of the fertilizer can be effectively saved, the nitrogen fertilizer utilization rate is increased, environmental pollution is reduced, and sustainable development of the sugarcane industry is facilitated.

The invention discloses new application of a florigen gene GmFT2a to improvement of biological nitrogen fixation efficiency of leguminous plants. The florigen gene GmFT2a is mainly expressed in laterdevelopment period of root nodules and is positioned in cell nucleus and cytoplasm of neoplastic cortical cells, so that the number and weight of the soybean root nodule are significantly increased, the activity of dinitrogenase is significantly improved, the nitrogen content and the phosphorus content of plants are increased, and the biomass of transgenic plants is finally increased. Efficient utilization of nitrogen and phosphorus nutrients of leguminous crops can be realized, gene resources are provided for high-yield molecular breeding as well as genetic improvement of photoperiodicity, and important guidance and practical significance in developing fertilize-reducing and effect-improving sustainable ecological agriculture are achieved.

The invention relates to a summer corn and summer peanut intercropping planting method and belongs to the technical field of crop planting. According to the summer corn and summer peanut intercropping planting method, three lines of corn are intercropped with four lines of peanuts, the corn line width is 55cm, the corn planting distance is 15cm, the peanut line width is 33-35cm, the peanut hill spacing is 14cm, the peanuts are planted in a ridging mode, the ridge width is 85cm, two lines of the peanuts are sown in each ridge, and the distance between the corn lines and the peanut ridges is 35cm. Three lines of the corn and four lines of the peanuts are arranged in an alternating mode, intercropping is carried out in a wide width mode, the corn line width, the corn planting distance, the peanut line width, the peanut hill spacing, and the distance between the corn lines and the peanut ridges are strictly limited, and therefore the total output value is remarkably improved.

The invention discloses a compound microorganism fertilizer suitable for leguminous plants. The compound microorganism fertilizer is prepared from, by mass, humic acid, yeast powder, purple non-sulfurphotosynthetic bacteria, organophosphorus bacteria, potassium sulphate and calcium superphosphate. According to the compound microorganism fertilizer, the soil fertility can be improved, and throughthe synergistic effect between nitrogen-fixing bacteria and purple non-sulfur photosynthetic bacteria in the leguminous plants, the dinitrogenase activity of root nodules and the soil nitrogen fixingstrength can be improved; besides, the nitrogen-fixing bacteria can promote the phosphorus-dissolving capability of the organophosphorus bacteria, and formation of water-soluble phosphorus in soil isfacilitated; long-term improvement of the soil can be achieved, through the synergistic effect between the nitrogen-fixing bacteria and purple non-sulfur photosynthetic bacteria in the leguminous plants, increase of other soil microorganisms can be promoted, the microorganism environment in the soil is improved, and durable fertilizer efficiency is generated; the infection of pest diseases can belowered, and through the synergistic effect between the nitrogen-fixing bacteria and purple non-sulfur photosynthetic bacteria in the leguminous plants, the infection of soybean cyst nematode diseasesis alleviated.

The invention discloses a strain of Brevibacterium halotolerant with higher nitrogenase activity and its application in ecological restoration. The Brevibacterium halotolerans GDSD21 has a deposit number of CGMCC No. 12402 in the Center for General Microorganisms of the China Committee for Culture Collection of Microorganisms. The present invention isolates nitrogen-fixing bacteria from the rhizosphere soil of farmland crops, further screens strains with higher nitrogenase activity, and finally screens the high-efficiency nitrogen-fixing bacteria Brevibacterium halotolerant GDSD21, and the nitrogenase activity of the Brevibacterium halotolerant GDSD21 of the present invention is extremely high. Azotobacter rotundum ACCC11103 is commonly used in the production of microbial fertilizers. It has broad application prospects in improving soil, improving soil fertility, ecological restoration of wasteland, inoculants for vegetable factory seedlings, nitrogen-fixing microbial agents and bio-organic fertilizer production.

The invention relates to an azotobacter chroococcum mutant with good nitrogen fixation capability and an application thereof. The azotobacter chroococcum mutant is azotobacter chroococcum A2820, is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 24, 2020, and has a preservation number of CGMCC No.20443; and the nitrogenase activity of the azotobacter chroococcum mutant is 210-280 nmol.mg<-1>.h<-1>. The azotobacter chroococcum mutant disclosed by the invention is a high-enzyme-activity mutant strain which is mutated by ARTP for 28 seconds and is determined and screened by an acetylene reduction method, and has good nitrogen fixation capability; and the genetic stability of the azotobacter chroococcum mutant is determined by multiple subculture.

The invention discloses novel application of florigen gene GmFT5a in promoting biological nitrogen fixation for leguminous plants and improving nitrogen and phosphorus nutrient efficiency. The florigen gene GmFT5a starts expressing upon rapid development of root nodules and locating at nuclei and cytoplasm of cortical cells of the root nodules, thereby significantly increasing the quantity and weight of soybean root nodules and dinitrogenase activity under both long and short durations of sunshine, increasing nitrogen and phosphorus contents in plants, and finally increasing biomass quantity of transgenic plants. Nitrogen and phosphorus nutrition in leguminous crops can be efficiently utilized; candidate gene resources are provided for high-yield molecular breeding with good photoperiod eurytopicity; the florigen gene GmFT5a has significant theoretical and practical significances in the development of environment-friendly sustainable ecological agriculture.

The invention discloses a Geomonas niftrogeniifigens RF4 and its application. It belongs to the field of microbial technology. Geomonas RF4 is strictly anaerobic, gram-negative, motile, and flagella all over the body. Colonies are red and round. Contactase and oxidase reactions were negative. Strain RF4 has the ability to produce alkaline phosphatase, acid phosphatase and naphthol-AS-BI-phosphohydrolase. For the first time, Geomonas was found to have both iron-reducing and nitrogen-fixing functions. It has good application prospects in agricultural production to drive rice growth, reduce nitrogen fertilizer application, and reduce environmental pollution.