48results about How to "Increase nitrogenase activity" patented technology

The invention discloses a brevibacterium halotolerans with relatively high azotase activity and application thereof in ecological restoration. The brevibacterium halotolerans GDSD21 is collected in the China General Microbiological Culture Collection Center with the collection number of CGMCC No.12402. Nitrogen-fixing bacteria are separated from rhizosphere soils of field-crop roots, further strains with relatively high azotase activity are screened, and finally the efficient nitrogen-fixing bacterium brevibacterium halotolerans GDSD21 is screened. The azotase activity of the brevibacterium halotolerans GDSD21 is obviously higher than that of common production strain azotobacter chroococcum ACCC11103 for microbial fertilizers, and the brevibacterium halotolerans GDSD21 has extensive application prospects in soil improvement, increasing of the soil fertility, ecological restoration of a virgin land, and production of industrialized seedling-rearing inoculation agents, nitrogen-fixing microbial agents and bio-organic fertilizers for vegetables.

The invention provides a microbial soil improving agent for restoring petroleum polluted soil. A product is prepared from a degradation bacterial agent, an additive and a biosurfactant, wherein the degradation bacterial agent is prepared from pseudomonas, bacillus, Acinetobacter, microzyme, phosphate-solubilizing bacteria, azotobacterial, nitrobacteria and photosynthetic bacteria; the additive isprepared from the following ingredients in parts by weight: 20 to 35 parts of wormcast, 80 to 120 parts of bentonite, 1 to 10 parts of chitosan, 10 to 20 parts of kaolin, 5 to 9 parts of borax, 25 to35 parts of crop stalks, 10 to 15 parts of rice hull powder, 10 to 15 parts of dry seaweed, 1 to 5 parts of organic acid, 5 to 10 parts of vermiculite, 10 to 20 parts of shell powder and 20 to 25 parts of compound amino acid. The microbial soil improving agent for restoring petroleum polluted soil has the advantages that the soil fertility is improved; the petroleum in the soil can be degraded; the goal of governing the petroleum polluted soil is achieved.

The invention discloses a RY2 strain of a root nodule nitrogen-fixing bacterial strain system and application thereof. The strain (Bradyrhizobium yuanmingense RY2) is preserved in the China Center for Type Culture Collection (CCTCC) on 13th November in 2009. The preservation number is CCTCC NO:M 209266. The RY2 strain of the root module nitrogen-fixing bacterial strain system can exclusively greatly improve the nitrogenase activity of TPRC2, so that the nitrogenase activity of the TPRC2 is over three times higher than that of TPRC5 and can achieve higher nitrogenase activity by using a few root nodules. The yield of stylosanthes guianensis can be improved by over 30 to 40 percent by inoculating the root nodule bacteria, and the root nodule bacteria can be suitable for intercropping of stylosanthes guianensis TPRC2 and provides a large number of nitrogen for the stylosanthes guianensis, crops and soil. Meanwhile, the RY2 has strong salt tolerance, can grow in the environment that the NaCl concentration is higher than 0.25mol/L, can be planted in the salinized soil and has better effects of improving the yield of the stylosanthes guianensis and utilizing and improving the salinized soil.

The invention discloses a cysteine protease gene for regulating and controlling root nodule senescence, and a preparation method and application thereof. According to the preparation method disclosed by the invention, a Japan lotaustralin genomic sequence library is compared by utilizing the known cysteine protease gene of astragalus smicus; after the Japan lotaustralin genomic sequence library is analyzed and compared on the internet in a detailed manner, a primer having strong specificity is designed to carry out gene cloning by taking initiation codon as the starting point and termination codon as the finishing point; in addition, due to sequencing analysis and comparing analysis, a cloned fragment proves that the cysteine protease gene for regulating and controlling root nodule senescence is successfully cloned from the lotaustralin genomic sequence and cDNA (complementary deoxyribonucleic acid). The invention discloses application of the cysteine protease gene on leguminous plants; the expression mode of a gene promoter and the functions of the gene in the root nodule senescence process are researched by utilizing a histochemical localization technology. Compared with contrast plants, the results show that the root nodule of an RNAi (ribonucleic acid interference) plant is enlarged; the nitrogen fixation enzyme activity is increased; senescence of the root nodule is delayed; the effective nitrogen fixation time of the root nodule is prolonged; growth of the plant is more vigorous; the cysteine protease gene disclosed by the invention has application prospect on leguminous crops.

A biological fertilizer for bamboo forest is prepared from Paenibacillus polymyxa WG-7 and/or Bacillus licheniformis WG-8, and matrix.

The invention discloses a Sclerotinia sclerotiorum-antagonizing nitrogen-fixing spore bacterium and its application. The nitrogen-fixing spore bacterium is Bacillus sp. GDSD212, which is preserved in General microbiological center of China Committee for Culture Collection of Microorganisms with a preservation number of CGMCC No.5035. The Bacillus sp. GDSD212 CGMCC No.5035 provided in the invention has high nitrogen-fixing activity, can effectively antagonize Sclerotinia sclerotiorum, and has a broad application prospect in production of nitrogen-fixing microorganism bacterial agents and bio-organic fertilizers.

The invention belongs to the technical field of microorganisms, and relates to a mutant strain as well as a construction method and application thereof. The pseudomonas stutzeri mutant strain is pseudomonas stutzeri DSM4166-TP (nifR), is preserved in China General Microbiological Culture Collection Center on December 20, 2019, and has a preservation number of CGMCC No.19081. The pseudomonas stutzeri mutant strain has a preservation number of CGMCC No.19081. The pseudomonas stutzeri mutant strain has the beneficial effects that compared with the existing wild type DSM4166 strain, the pseudomonas stutzeri mutant strain has the advantages that the nitrogenase synthesis gene expression level is greatly improved, and the activity of the produced nitrogenase is also greatly higher than that of the wild type DSM4166 strain. The pseudomonas stutzeri mutant strain disclosed by the invention can be industrially produced on a large scale and has a wide commercial value.

The invention discloses a wheat rhizosphere azotobacter for antagonism of various pathogenic fungi and application of the wheat rhizosphere azotobacter. The wheat rhizosphere azotobacter is Paenibacillus sp. GD641, and has the collection number of CGMCC No.5042 in China General Microbiological Culture Collection Center. The Paenibacillus sp. GD641 with the collection number of CGMCC No.5042 has effective antagonism on Gibberella zeae, Sclerotinia sclerotiorum and Verticillium dahlia of crops, and has wide application prospect in microbial azotobacter and biological organic fertilizer production.

The invention is related to a symbiotic system comprising a plant of the Leguminosae family, and a bacterium of the rhizobium family, wherein in said bacterium a gene coding for a flavohemoglobin protein is over expressed. The present invention is also related to a method for delaying the senescence of symbiotic nodules, comprising inoculating a plant of the Leguminosae family with a bacterium of the rhizobium family, wherein said rhizobium overexpresses a gene coding for a flavohemoglobin protein. A method for cultivating a plant of the Leguminosae family is also disclosed.

The invention relates to the technical field of microorganisms, and particularly relates to Sphingobium abikonense HP10 and application thereof. The Sphingobium abikonense HP10 is preserved in the China General Microbiological Culture Collection Center on January 13, 2021, and the preservation number is CGMCC No. 21618. The strain has high nitrogenase activity, can degrade spirodiclofen, can inhibit bacteria and secrete indoleacetic acid. The Sphingobium abikonense HP10 can be used for microbial nitrogen fertilizer, can also serve as a soil residual pesticide degrading agent, can promote plant growth and has great significance in planting hybrid paper mulberry in northwest regions.

The invention relates to the technical field of sugarcane planting, and particularly discloses a sugarcane weight-losing and efficiency-improving planting method which comprises the following steps: (1) soil preparation; (2) seed stem treatment; (3) sugarcane planting; (4) field management; (5) harvesting. According to the planting method, a proper amount of nitrogen fertilizer is intensively applied in the early growth stage of sugarcane, phosphorus-potassium fertilizer and microelement fertilizer are cooperatively applied, the PH value of soil and water supply are adjusted, meanwhile, nitrogen-fixing bacteria liquid is adopted for soaking seeds, compound microorganism fertilizer is adopted as base fertilizer, early-stage growth of sugarcane can be accelerated, sugarcane plants can form strong root systems and robust overground stem leaves, and the yield of sugarcane is increased. Fertilization is not needed in the large growth period (elongation period) after the sugarcane is closed, biological nitrogen fixation of the sugarcane is promoted, nitrogen nutrition needed by growth of the sugarcane is provided, and compared with a traditional'three-attack and one-supplement 'fertilization method, implementation of the fertilizer can be effectively saved, the nitrogen fertilizer utilization rate is increased, environmental pollution is reduced, and sustainable development of the sugarcane industry is facilitated.

The invention discloses new application of a florigen gene GmFT2a to improvement of biological nitrogen fixation efficiency of leguminous plants. The florigen gene GmFT2a is mainly expressed in laterdevelopment period of root nodules and is positioned in cell nucleus and cytoplasm of neoplastic cortical cells, so that the number and weight of the soybean root nodule are significantly increased, the activity of dinitrogenase is significantly improved, the nitrogen content and the phosphorus content of plants are increased, and the biomass of transgenic plants is finally increased. Efficient utilization of nitrogen and phosphorus nutrients of leguminous crops can be realized, gene resources are provided for high-yield molecular breeding as well as genetic improvement of photoperiodicity, and important guidance and practical significance in developing fertilize-reducing and effect-improving sustainable ecological agriculture are achieved.

The invention discloses a compound microorganism fertilizer suitable for leguminous plants. The compound microorganism fertilizer is prepared from, by mass, humic acid, yeast powder, purple non-sulfurphotosynthetic bacteria, organophosphorus bacteria, potassium sulphate and calcium superphosphate. According to the compound microorganism fertilizer, the soil fertility can be improved, and throughthe synergistic effect between nitrogen-fixing bacteria and purple non-sulfur photosynthetic bacteria in the leguminous plants, the dinitrogenase activity of root nodules and the soil nitrogen fixingstrength can be improved; besides, the nitrogen-fixing bacteria can promote the phosphorus-dissolving capability of the organophosphorus bacteria, and formation of water-soluble phosphorus in soil isfacilitated; long-term improvement of the soil can be achieved, through the synergistic effect between the nitrogen-fixing bacteria and purple non-sulfur photosynthetic bacteria in the leguminous plants, increase of other soil microorganisms can be promoted, the microorganism environment in the soil is improved, and durable fertilizer efficiency is generated; the infection of pest diseases can belowered, and through the synergistic effect between the nitrogen-fixing bacteria and purple non-sulfur photosynthetic bacteria in the leguminous plants, the infection of soybean cyst nematode diseasesis alleviated.

The invention relates to an azotobacter chroococcum mutant with good nitrogen fixation capability and an application thereof. The azotobacter chroococcum mutant is azotobacter chroococcum A2820, is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 24, 2020, and has a preservation number of CGMCC No.20443; and the nitrogenase activity of the azotobacter chroococcum mutant is 210-280 nmol.mg<-1>.h<-1>. The azotobacter chroococcum mutant disclosed by the invention is a high-enzyme-activity mutant strain which is mutated by ARTP for 28 seconds and is determined and screened by an acetylene reduction method, and has good nitrogen fixation capability; and the genetic stability of the azotobacter chroococcum mutant is determined by multiple subculture.