6results about How to "Increase nitrogenase activity" patented technology

The invention discloses a CAPE polypeptide released by a protein coded by a soybean disease course related gene GmPR1-6 and application of the CAPE polypeptide. The nucleotide sequence of the soybean disease course related gene is as shown in SEQ ID NO.1, the amino acid sequence of protein coded by the soybean disease course related gene is as shown in SEQ ID NO.2, and the sequence of CAPE polypeptide released at the C terminal of the protein is as shown in SEQ ID NO.9. The soybean disease course related gene GmPR1-6 provided by the invention is a gene coding a core protein participating in soybean immune defense reaction, the C tail end of the GmPR1-6 protein can be sheared to release CAPE polypeptide, the CAPE polypeptide is externally applied to weaken early immune defense reaction triggered by soybean during infection of rhizobium, the weight and size of a single root nodule are increased, and the disease resistance of the soybean is improved. The symbiotic nodulation and nitrogen fixation capabilities of the soybeans are improved, and the method has potential application value in planting of leguminous plants.

The application discloses a method for improving the soil fertility of traditional Chinese medicinal materials by using nitrogen-fixing cyanobacterial biofilm. The method comprises six steps of cyanobacterial culture, biofilm substrate construction, biofilm formation, biofilm particle preparation, soil application and ecological regulation in sequence. Blue-green algae strains with high nitrogen-fixing activity such as Nostoc and Lyngbya are screened, high-activity culture solution is obtained by accurate culture, a biofilm substrate is prepared by compounding porous minerals, organic carbon sources and bio-polymer adhesives, the blue-green algae culture solution is inoculated and the environment is regulated to form a three-dimensional network blue-green algae biofilm, and 2-10mm blue-green algae biofilm particles are obtained by granulation, and the particles are applied to the 0-5cm surface layer of the soil of the traditional Chinese medicinal materials at an application amount of 200-500kg / hm 2 The application amount is 200-500kg / hm The blue-green algae form a biological crust structure. The application forms a synergistic ecological system by means of the photosynthetic nitrogen fixation of blue-green algae and the improvement of soil structure by extracellular polysaccharides and rhizosphere microorganisms.

The invention relates to a method for promoting growth, nodulation and nitrogen fixation of indigofera pseudotinctoria by utilizing rhizobium D303 and a bacterial solution thereof. The biological preservation number of the rhizobium sp. D303 is CCTCC (China Center for Type Culture Collection) NO: M20252803. Pot culture tie-back tests prove that the indigofera pseudotinctoria seedlings inoculated with the rhizobium are obviously superior to a control group in the aspects of plant height, root length, overground dry weight, underground dry weight, root nodule number, root nodule weight, root nodule diameter, nitrogenase activity, antioxidant enzyme activity and the like. The rhizobium strain provided by the invention has efficient nodulation and nitrogen fixation capability and excellent growth promoting characteristic, obviously improves the growth speed, nodulation capability and nitrogen fixation efficiency of the indigofera pseudotinctoria, reduces the dependence on chemical nitrogen fertilizer, and has a broad application prospect in development of special biological bacterial fertilizer for indigofera pseudotinctoria. The method has important ecological and economic benefits for promoting the application of the indigofera pseudotinctoria in ecological restoration and feed production, and is suitable for slope greening, water and soil conservation and barren mountain ecological restoration projects.

The application discloses a plant growth regulator. By externally applying the plant growth regulator to leguminous plants, the leguminous plants can maintain high tolerance under weak light conditions such as dense planting, strip complex planting, under forest, facility greenhouse or cloudy and rainy days, and the implementation is simple, labor is saved, and the plant growth regulator can be used for mechanical operation.

The invention provides a foliar fertiliser that includes an extract of methylotrophic microorganisms obtained from a first initial process of fermenting live methylotrophic microorganisms using methanol as the only source of carbon or energy and a second process that includes the stages of cell disruption by physical, chemical or enzymatic methods, separating the soluble parts from the insoluble parts by means of tangential filtration or centrifugation and, optionally, concentrating by means of filtration or atomisation.

The present invention discloses the application of energy receptor SnRK1α4 protein and related biological materials in regulating symbiotic nitrogen fixation in plants. The present invention provides a protein derived from Medicago truncatula, named SnRK1α4 protein, which is the protein shown in SEQ ID NO: 1. Nucleic acid molecules encoding SnRK1α4 protein also belong to the protection scope of the present invention. The nucleic acid molecule can specifically be the SnRK1α4 gene. The present invention also protects the application of SnRK1α4 protein or nucleic acid molecules encoding SnRK1α4 protein: (c1) positively regulating the symbiotic nitrogen fixation ability of plants; (c2) positively regulating the symbiotic nitrogen fixation ability of plants and nitrogen-fixing bacteria; (c3) positively regulating the growth performance of plants in low-nitrogen environments. The present invention provides valuable evidence for the study of nitrogen fixation mechanism of leguminous plants, provides important gene resources for the cultivation of new varieties of efficient nitrogen-fixing plants, and has important application prospects in agricultural production.