Application method of immobilized microorganisms in livestock breeding wastewater treatment

A technology for immobilizing microorganisms and livestock and poultry breeding wastewater, which is applied in animal husbandry wastewater treatment, biological water/sewage treatment, biological treatment devices, etc. It can solve the problems of insufficient removal rate of total nitrogen and total phosphorus and unsatisfactory treatment effect, etc. , to achieve good degradation performance, enhance antioxidant capacity, and improve stress resistance

Inactive Publication Date: 2019-03-12
SHANDONG YIAN BIOLOGICAL ENG CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing reports on the industrialized treatment of aquaculture wastewater, most of the organic matter and suspended matter can be removed after anaerobic treatment, but in the aerobic treatment process, the removal rate of total nitrogen and total phosphorus is not high enough, and the treatment effect is not good. ideal question

Method used

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  • Application method of immobilized microorganisms in livestock breeding wastewater treatment
  • Application method of immobilized microorganisms in livestock breeding wastewater treatment
  • Application method of immobilized microorganisms in livestock breeding wastewater treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Preparation of seed solution: Inoculate the strains of Alcaligenes faecalis, Pseudomonas stutzeri and Acinetobacter piteri on nutrient agar medium by plate coating method, culture at 30°C for 48-72 hours, pick Take a single colony that grows well on the plate, inoculate it into nutrient broth medium under aseptic conditions, and culture it on a shaking table at 30°C and 160r / min for 48 hours; inoculate the strain of Bacillus coagulans on LB solid On the culture medium, culture at a constant temperature of 35°C for 48-72h, pick a single colony that grows well on the plate, inoculate it into LB liquid medium under aseptic conditions, and shake it at 35°C and 180r / min for 24h;

[0035] In the same way, inoculate the strains of Penicillium chrysogenum on PDA medium, culture at a constant temperature of 28°C for 4-6 days, wash the spores with sterile physiological saline, shake them evenly, and form a spore suspension, and inoculate according to the inoculation amount of ...

Embodiment 2

[0037] Example 2 Effect of embedding material on the performance of immobilized microbial gel balls

[0038] Preparation of immobilized microorganisms: the microbial fermentation broth in Example 1 was centrifuged with a centrifuge at 7500r / min for 15 minutes to collect the bacteria and then resuspended in distilled water, then fully mixed with the composite embedding material at a volume ratio of 1:20, Slowly drop into saturated boric acid and 3% calcium chloride solution to cross-link to form immobilized microbial gel balls, put them in a refrigerator at 4°C for 24 hours, take out the gel balls and wash them with 9% NaCl solution, and then wash them with distilled water for 2 -3 times.

[0039] Determination of mass transfer performance: Add 100mL of basic salt medium to a 250mL conical flask, add immobilized microbial gel balls into it, then add 4-5 drops of red ink, take it out after a while, rinse it with clean water, and observe the condensation. The range of discolorat...

Embodiment 3

[0050] Using the best composite embedding material in Example 2 to prepare immobilized microbial gel balls, according to the technical scheme of the present invention, the wastewater collected by a livestock and poultry breeding wastewater treatment center in Lianyungang City, Jiangsu Province was treated in a small test. For sedimentation and anaerobic treatment, the test consisted of the following steps:

[0051] (1) Induction culture of immobilized microorganisms: put the prepared immobilized microorganism gel balls into a three-neck flask, add induction medium (composition: 14g / L sucrose, 5.3g / L peptone) accounting for 40% of the volume of the flask , urea 3.6g / L, NaCl 3.8g / L, KH 2 PO 4 1.5g / L, MgSO 4 ·7H 2 O0.75g / L, glutathione 16mg / L, sodium citrate 7.0mg / L, sodium nitroprusside 50μmol / L, betaine 1.8mg / L, fulvic acid 0.26g / L, kandol 0.15mg / L L, pH value is natural), feed purified air and cultivate at a temperature of 30°C for 48 hours, and feed high-purity hydrogen ...

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Abstract

The invention discloses an application method of immobilized microorganisms in livestock breeding wastewater treatment. The technical flow comprises the following steps: (1) preparation of microbial fermentation broth; (2) preparation of the immobilized microorganisms; (3) induced culture of the immobilized microorganisms; (4) habituated culture; and (5) breeding wastewater treatment. According tothe invention, various microorganism symbiotic systems are constructed, and microorganisms can cooperate to effectively degrade organic substances, nitrogen, phosphorus and heavy metal pollutants inthe livestock breeding wastewater; a composite embedding material is adopted to immobilize the microorganisms, and a unique micro environment is formed to have a good shielding effect on outside invasion; resistance and tolerance of the immobilized microorganisms on the breeding wastewater are further improved through induced culture and habituated culture; and when the immobilized microorganismsare applied to a wastewater treatment system, stability is relatively high within a service period, and a good degradation effect is achieved on the livestock breeding wastewater, and therefore, a foundation is laid for practical application of the immobilized microorganisms in wastewater treatment.

Description

technical field [0001] The invention belongs to the technical field of environmental protection wastewater treatment, and in particular relates to an application method of immobilized microorganisms in livestock and poultry breeding wastewater treatment. Background technique [0002] In recent years, with the development of the economy and the improvement of people's living standards, people's demand for livestock and poultry products such as meat, eggs, milk, etc. has gradually increased, making my country's large-scale and intensive livestock and poultry breeding industry flourish. The production level of livestock and poultry has also been greatly improved, but it has also caused serious environmental pollution problems. According to the survey, the chemical oxygen demand (COD) emitted by the livestock and poultry breeding industry reached 12.6826 million tons, accounting for 41.9% of the country's total COD emissions, and the emissions of nitrogen and phosphorus were 1.02...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F3/12C02F103/20C02F101/30C02F101/16C02F101/20C02F101/10
CPCC02F3/1263C02F3/34C02F2101/105C02F2101/16C02F2101/20C02F2101/30C02F2103/20C02F2203/004C02F2203/006Y02W10/10
Inventor 杨鸿羽卢波
Owner SHANDONG YIAN BIOLOGICAL ENG CO LTD
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