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Aspergillus niger JXJ A01 with potassium and phosphate dissolving and organophosphorus pesticide decomposing activity and application thereof

A JXJA01, organophosphorus pesticide technology, applied in the application, organic fertilizer, potash fertilizer and other directions, to achieve the effect of improving and repairing farmland soil and reducing organophosphorus pesticide residues

Active Publication Date: 2017-12-29
JIUJIANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many microorganisms can dissolve potassium ore and turn insoluble ineffective potassium into soluble available potassium. At present, most of the researches are potassium-dissolving bacteria, and filamentous molds are rarely reported.

Method used

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  • Aspergillus niger JXJ A01 with potassium and phosphate dissolving and organophosphorus pesticide decomposing activity and application thereof
  • Aspergillus niger JXJ A01 with potassium and phosphate dissolving and organophosphorus pesticide decomposing activity and application thereof
  • Aspergillus niger JXJ A01 with potassium and phosphate dissolving and organophosphorus pesticide decomposing activity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] 1 Screening of Aspergillus phosphorolyticus

[0026] Solid plate screening: The forest soil collected from Jiujiang City, Jiangxi Province was gradiently diluted with sterile water, and 0.2mL of the appropriate gradient dilution was applied to the phosphorus-dissolving solid culture of the industry standard NY / T 1847-2010 of the Ministry of Agriculture of China. Based on (glucose 10g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, MgSO 4 ·7H 2 O 0.3g, KCl 0.3g, FeSO 4 4H 2 O 0.036g, MnSO 4 4H 2 O 0.03g, calcium phosphate or calcium phytate 10g, agar 18g, distilled water 1000mL, pH7.0), and after culturing in a 28°C incubator for 1-7d, observe the growth of bacteria and whether there is phosphorus dissolving around each colony Circle, select the strain JXJ A01 ( figure 1 ) for follow-up research.

[0027] 2 Identification of Aspergillus niger JXJ A01

[0028] According to the culture characteristics, morphological characteristics, 18S rRNA gene sequence and ITS sequence anal...

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Abstract

The invention relates to the technical field of microbe application, particularly to an aspergillus niger JXJ A01 with potassium and phosphate dissolving and organophosphorus pesticide decomposing activity and application of the aspergillus niger JXJ A01 with the potassium and phosphate dissolving and organophosphorus pesticide decomposing activity to dissolution of insoluble potassium, organophosphorus and inorganic phosphorous, decomposition of organophosphorus pesticides and preparation of microbial fertilizers. The aspergillus niger JXJ A01 is stored in China General Microbiological Culture Collection Center with a preservation number of CGMCC No. 13775. The aspergillus niger JXJ A01 is high in dissolving effects on insoluble phosphorous and potassium ore powder and can convert the insoluble phosphorous and the potassium ore fertilizer into soluble phosphorous and potassium absorbable for plants and degrade the organophosphorus pesticide of omethoate into non-toxic soluble phosphate radicals to reduce pesticide concentration and increase the content of soluble phosphorous in soil. The aspergillus niger JXJ A01 with the potassium and phosphate dissolving and organophosphorus pesticide decomposing activity can be applied to increasing the content of soluble effective potassium and phosphorus in soil and repair pollution caused by the organophosphorus pesticides to soil.

Description

technical field [0001] The present invention relates to the technical field of microbial application, in particular to the application of Aspergillus niger JXJ A01 capable of converting poorly soluble potassium, organic phosphorus and inorganic phosphorus into soluble potassium and phosphorus in microbial fertilizers, and to the application of Aspergillus niger JXJ A01 capable of degrading organic phosphorus pesticides. Application of Aspergillus JXJ A01 in reducing organic phosphorus pesticide residues in farmland, improving and repairing farmland soil. Background technique [0002] Phosphorus is an indispensable element for the normal growth and development of plants. More than 95% of the phosphorus in the soil is insoluble phosphorus that is difficult for plants to directly absorb and utilize. About 74% of the cultivated land in my country is deficient in phosphorus. The application of organic and chemical phosphorus fertilizers is the main source of soil phosphorus. How...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05G1/00A62D3/02C09K17/14A62D101/26A62D101/45A62D101/40C09K101/00C12R1/685
CPCA62D3/02A62D2101/26A62D2101/40A62D2101/45C05B17/00C09K17/14C09K2101/00C05G5/45C12N1/145C12R2001/685C05D1/04C05D9/02C05F3/00C05F11/08C05G5/12C05F5/002C05F11/00
Inventor 张炳火李汉全
Owner JIUJIANG UNIVERSITY
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