Surface modified lipidosome as well as preparation method and application thereof

A technology of surface modification and liposome, which is applied in the biological field to achieve the effect of improving stability and easy storage

Inactive Publication Date: 2017-06-13
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Using layer-by-layer self-assembly technology to deposit polyelectrolytes (such as polylysine, sodium alginate and chitosan, etc.) on the surface of liposomes to form a protective film can improve its storage stability and digestion stability, but it has not yet been established. There are related reports about Lactobacillus acidophilus S-layer protein modified liposomes

Method used

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  • Surface modified lipidosome as well as preparation method and application thereof
  • Surface modified lipidosome as well as preparation method and application thereof
  • Surface modified lipidosome as well as preparation method and application thereof

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Embodiment 1

[0031] A kind of surface modification liposome, described liposome is soybean lecithin and cholesterol mix by mass ratio 5:1, and is modified by Lactobacillus acidophilus S-layer protein; Wherein, Lactobacillus acidophilus S-layer protein and The mass ratio of liposomes was 1:1.44.

[0032] A method for preparing surface-modified liposomes, comprising the following steps:

[0033] (1) Crude extraction of Lactobacillus acidophilus S-layer protein

[0034] Lactobacillus acidophilus (Lactobacillus acidophilus) CICC6074 (China Industrial Microbial Culture Collection Management Center) was cultured with liquid MRS medium to the logarithmic phase of growth, and the bacterial precipitate was collected by centrifugation. The bacterial cell was adjusted to OD600 of 1.0 with normal saline, and the LiCl solution is mixed, the concentration of acidic LiCl is 5mol / L, pH=2.0, the volume ratio of the bacterial suspension to the acidic LiCl solution is 35:1, the incubation temperature of the...

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Abstract

The invention discloses a surface modified lipidosome as well as a preparation method and application thereof. The lipidosome is prepared by mixing soya bean lecithin with cholesterol at mass ratio of (7.5-2.5) to 1 and is modified by lactobacillus acidophilus S-albumen. (1) The lactobacillus acidophilus S-albumen is adopted for modifying the lipidosome surface, so that the stability and storage of the lipidosome can be promoted. (2) The lipidosome has tolerance to gastrointestinal tract protease and the wrapped effective constituents can smoothly reach target tissues so as to effectively exert drug effects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a liposome and its preparation method and application, in particular to a liposome modified with Lactobacillus acidophilus S-layer protein and its application in the fields of functional food and medicine. Background technique [0002] As a new type of drug dosage form, liposome has become a research hotspot due to its unique targeting, excellent controllable release and less drug dosage. However, although liposomes can protect the encapsulated drug, they are easily oxidized in vitro, causing liposome membrane rupture, content leakage, poor stability, difficult storage, and easy degradation by some enzymes in the body. The intestinal tract is easily eroded by gastrointestinal fluid, and cannot reach the target tissue to effectively exert the drug effect. In recent years, improving the stability of liposomes has become a hot topic in development. Using layer-by-layer self-assembly tec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K47/64A23L33/135A23L33/115
Inventor 郭宇星潘道东薛逸秋
Owner NANJING NORMAL UNIVERSITY
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