High-efficiency phosphate-solubilizing aspergillus japonicus with heavy metal tolerance

A technology of Aspergillus and heavy metals, applied in the field of environmental biology

Inactive Publication Date: 2014-03-05
BEIJING FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no relevant report on the research on high-efficiency phospho

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  • High-efficiency phosphate-solubilizing aspergillus japonicus with heavy metal tolerance
  • High-efficiency phosphate-solubilizing aspergillus japonicus with heavy metal tolerance

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Embodiment 1

[0015] Example 1: Screening and Identification of Phosphorus Solubilizing Aspergillus japonicus

[0016] The Aspergillus japonicus strain of this embodiment is isolated and screened from the surface soil of a lead-zinc mine in Huayuan County, Xiangxi Prefecture, Hunan Province. The screening is carried out as follows: the collected soil is immediately brought back to the laboratory, air-dried, ground, and sieved, and 10.0 g of the sieved soil is placed in 100 ml of sterile water with glass beads to make a soil suspension , the concentration of this suspension is 10 -1 ,. Place the suspension on a constant temperature oscillator at 150 rpm for 30 minutes, take it out and let it stand for a while, take 10ml from the sample suspension and add it to 90ml of sterile water, and the concentration of the suspension is 10 -2 , and then perform 10-fold serial dilution in this way until the concentration of the diluent is 10 -4 until. take 10 -4 Concentration of bacterial suspensio...

Embodiment 2

[0017] Embodiment 2: Phosphorus-solubilizing test of phosphorus-solubilizing Aspergillus japonicus to insoluble phosphate

[0018] Select five insoluble phosphates and make the following medium

[0019] Phosphate-dissolving medium 1: glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, MgSO 4 ·7H 2 O 0.3g, NaCl 0.3g, KCl 0.3g, FeSO 4 0.03g, MnSO 4 ·H 2 O 0.03g, yeast powder 0.5g, Ca 3 (PO 4 ) 2 10g, distilled water l000ml, pH7.0.

[0020] Phosphate-dissolving medium 2: Magnesium hydrogen phosphate (MgHPO 4 ) instead of Ca in phosphate-solubilizing medium 1 3 (PO 4 ) 2 , other components and contents remain unchanged.

[0021] Phosphate-dissolving medium 3: zinc phosphate (Zn 3 (PO 4 ) 2 Instead of Ca in Phosphorus Solubilizing Medium 1 3 (PO 4 ) 2 , other components and contents remain unchanged.

[0022] Phospholytic medium 4: with aluminum phosphate (AlPO 4 ) instead of Ca in phosphate-solubilizing medium 1 3 (PO 4 ) 2 , other components and contents remain uncha...

Embodiment 3

[0029] Embodiment 3: the dynamic change of phosphorus solubilizing property of Aspergillus japonicus

[0030] The above strains were inoculated on a PDA plate, and after culturing for 5 days, a spore suspension was prepared with sterile water, and the concentration of the spore suspension was determined to be 1.6×10 by hemocytometer. 7 spores / mL, inoculated into 50ml modified Montina liquid medium according to the inoculation amount of 1mL / bottle, and set as blank control group with no inoculation at the same time, add 1ml sterile water in each bottle of blank group, each treatment Repeat for three bottles. Place in a shaker at 28° C., shake culture at 150 rpm, detect the content of soluble phosphorus in the supernatant and the pH value of the medium every 24 hours, and measure continuously for 14 days. see results figure 1 shown.

[0031] Depend on figure 1 It can be seen that the phosphorus-dissolving ability of the above-mentioned strains has been tested for 14 days. ...

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Abstract

The invention discloses high-efficiency phosphate-solubilizing aspergillus japonicus with heavy metal tolerance. The high-efficiency phosphate-solubilizing aspergillus japonicus with the heavy metal tolerance is classified and named as aspergillus japonicus, and is collected in China General Microbiological Culture Collection Center; the collection number is CGMCC No.7700. The invention also discloses the tolerance of the high-efficiency phosphate-solubilizing aspergillus japonicus to heavy metals. The high-efficiency phosphate-solubilizing aspergillus japonicus has an extremely good dissolving effect on insoluble phosphates, such as tricalcium phosphate, magnesium phosphate and aluminum phosphate in the situation of liquid shake flask culture; the dissolving effects on the three types of insoluble inorganic phosphorus reach 100 percent. The aspergillus japonicus has certain tolerance to the heavy metals after being incubated in liquid culture solutions which contain the heavy metals such as Pb<2+>, Zn<2+>, Cr<2+>, Mn<2+>, Cu<2+>, As<5+> and Cd<2+>; particularly, the tolerated concentrations of the Pb<2+>, the Zn<2+>, the Cr<2+> and the Mn<2+> reach 2,000 mg/L respectively. Therefore, the high-efficiency phosphate-solubilizing aspergillus japonicus with heavy metal tolerance can provide excellent strain resources for the development of heavy metal-resistant high-efficiency phosphate-solubilizing bacterium agents.

Description

technical field [0001] The invention belongs to the field of environmental biotechnology, and in particular relates to a strain of Aspergillus japonicus with the characteristics of high-efficiency phosphorus-dissolving and heavy-metal tolerance. Background technique [0002] Phosphorus is one of the essential nutrients for plants, and 74% of the cultivated land in my country is deficient in phosphorus. More than 95% of the phosphorus in the soil is in an invalid form, and it is difficult for plants to directly absorb and utilize it. The crop utilization rate of the applied phosphorus fertilizer is 5% to 25%, most of the phosphorus and Ca in the soil 2+ , Fe 3+ , Fe 2+ 、Al 3+ Combined to form insoluble phosphate. Improving phosphorus utilization has always been a concern of agronomists. Many factors affect the use efficiency of soil phosphorus, among which microorganisms have a great influence on the transformation and availability of soil phosphorus. A large number of...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/66
Inventor 不公告发明人
Owner BEIJING FORESTRY UNIVERSITY
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