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High-efficiency phosphate-solubilizing penicillium oxalicum with heavy metal tolerance characteristic

A technology of Penicillium oxalicum and Penicillium, applied in the field of environmental biology

Inactive Publication Date: 2014-03-05
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no relevant report on the research on the high-efficiency Penicillium oxalicum and its resistance to heavy metals in China

Method used

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  • High-efficiency phosphate-solubilizing penicillium oxalicum with heavy metal tolerance characteristic
  • High-efficiency phosphate-solubilizing penicillium oxalicum with heavy metal tolerance characteristic
  • High-efficiency phosphate-solubilizing penicillium oxalicum with heavy metal tolerance characteristic

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Experimental program
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Effect test

Embodiment 1

[0014] Embodiment 1: Screening and identification of Penicillium oxalicum phospholyticum

[0015] The Penicillium oxalicum strain of this embodiment is isolated and screened from the surface soil of a lead-zinc mine in Huayuan County, Xiangxi Prefecture, Hunan Province. The screening is carried out as follows: the collected soil is immediately brought back to the laboratory, air-dried, ground, and sieved, and 10.0 g of the sieved soil is placed in 100 ml of sterile water with glass beads to make a soil suspension , the concentration of this suspension is 10 -1 ,. Place the suspension on a constant temperature oscillator at 150 rpm for 30 minutes, take it out and let it stand for a while, take 10ml from the sample suspension and add it to 90ml of sterile water, and the concentration of the suspension is 10 -2 , and then perform 10-fold serial dilution in this way until the concentration of the diluent is 10 -4 until. take 10 -4 Concentration of bacterial suspension 0.2ml,...

Embodiment 2

[0016] Embodiment 2: Phosphorus-solubilizing test of Penicillium oxalicum to insoluble phosphate

[0017] Select five insoluble phosphates and make the following medium

[0018] Phosphate-dissolving medium 1: glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, MgSO 4 ·7H 2 O 0.3g, NaCl 0.3g, KCl 0.3g, FeSO 4 0.03g, MnSO 4 ·H 2 O 0.03g, yeast powder 0.5g, Ca 3 (PO 4 ) 2 10g, distilled water l000ml, pH7.0.

[0019] Phosphate-dissolving medium 2: Magnesium hydrogen phosphate (MgHPO 4 ) instead of Ca in phosphate-solubilizing medium 1 3 (PO 4 ) 2 , other components and contents remain unchanged.

[0020] Phosphate-dissolving medium 3: zinc phosphate (Zn 3 (PO 4 ) 2 Instead of Ca in Phosphorus Solubilizing Medium 1 3 (PO 4 ) 2 , other components and contents remain unchanged.

[0021] Phospholytic medium 4: with aluminum phosphate (AlPO 4 ) instead of Ca in phosphate-solubilizing medium 1 3 (PO 4 ) 2 , other components and contents remain unchanged.

[0022] Phosphate...

Embodiment 3

[0028] Embodiment 3: the dynamic change of Penicillium oxalicum phosphorus solubilizing property

[0029] The above strains were inoculated on a PDA plate, and after culturing for 5 days, a spore suspension was prepared with sterile water, and the concentration of the spore suspension was determined to be 1.6×10 by hemocytometer. 7 spores / mL, inoculated into 50ml modified Montina liquid medium according to the inoculation amount of 1mL / bottle, and set as blank control group with no inoculation at the same time, add 1ml sterile water in each bottle of blank group, each treatment Repeat for three bottles. Place in a shaker at 28° C., shake culture at 150 rpm, detect the content of soluble phosphorus in the supernatant and the pH value of the medium every 24 hours, and measure continuously for 14 days. see results figure 1 shown.

[0030] Depend on figure 1 It can be seen that the phosphorus-dissolving ability of the above-mentioned strains has been tested for 14 days. Exce...

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Abstract

The invention discloses a strain penicillium oxalicum with high-efficiency phosphate solubilizing characteristic, which is classified and named as Penicillium oxalicum. The penicillium oxalicum has been preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number: CGMCC NO.7699. The invention further discloses tolerance of high-efficiency phosphate-solubilizing penicillium oxalicum to heavy metals. The strain has strong dissolving effect on insoluble phosphate tricalcium phosphate, magnesium phosphate and aluminum phosphate under the condition of shake-flask liquid culture condition, the dissolving effect on the tricalcium phosphate and magnesium phosphate can reach 100%, and the dissolving effect on the aluminum phosphate can reach 95.5%. The penicillium oxalicum is respectively inoculated into liquid culture liquor containing heavy metals such as Pb<2+>, Zn<2+>, Cr<2+>, Mn<2+>, Cu<2+>, As<5+> and Cd<2+>, has strong resistance to the heavy metals, especially, the concentration of tolerance to the Pb<2+>, Zn<2+>, Cr<2+> and Mn<2+> can respectively reach 2000mg / L. Therefore, the penicillium oxalicum can provide excellent strain resources for developing heavy metal-resistant high-efficiency phosphate-solubilizing bacteria agents.

Description

technical field [0001] The invention belongs to the technical field of environmental biology, and in particular relates to a Penicillium oxalicum with high-efficiency phosphorus-dissolving and heavy-metal-tolerant characteristics. Background technique [0002] Phosphorus is one of the essential nutrients for plants, and 74% of the cultivated land in my country is deficient in phosphorus. More than 95% of the phosphorus in the soil is in an invalid form, and it is difficult for plants to directly absorb and utilize it. The crop utilization rate of the applied phosphorus fertilizer is 5% to 25%, most of the phosphorus and Ca in the soil 2+ , Fe 3+ , Fe 2+ 、Al 3+ Combined to form insoluble phosphate. Improving phosphorus utilization has always been a concern of agronomists. Many factors affect the use efficiency of soil phosphorus, among which microorganisms have a great influence on the transformation and availability of soil phosphorus. A large number of research resul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14B09C1/10C12R1/80
Inventor 不公告发明人
Owner BEIJING FORESTRY UNIVERSITY
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