The invention discloses a recombinant
plasmid of a GSDMD-N (gasdmermin D-N)
gene, an expression method in
mammary gland and an application, and belongs to the field of
genetic engineering. An expression recombinant
plasmid of the GSDMD-N
gene is constructed and enabled to be specifically expressed in
breast tissue and breast cells, so that
mastitis caused by
staphylococcus aureus infection is prevented and treated. GSDMD-N
protein is a polypeptide compound found in recent years, which can specifically recognize
biofilm component
cardiolipin of
bacteria and can be combined with
cardiolipin to form a plurality of
honeycomb-shaped pores, so that
bacteria are disintegrated and die. According to the technology, the exogenous
gene GSDMD-N is efficiently expressed in animal mammary glands througha specific
promoter WAP (whey acidic
protein) of
breast tissue by a mammary
bioreactor,
staphylococcus aureus causing cow
mastitis is killed, and accordingly, the cow
mastitis caused by
staphylococcus aureus infection is prevented and treated. The recombinant
plasmid and the recombinant attenuated
salmonella have the advantages of being efficient, safe, low in cost and the like, provide a a new approach for prevention and control of the cow mastitis caused by
staphylococcus aureus infection, and have good application prospect.