22 results about "Teichoic acid" patented technology

Provided are a postbiotic of Weizmannia coagulans, and a preparation method therefor, and a use thereof. The postbiotic comprises inactivated bacteria and / or lipoteichoic acid. The postbiotic can enhance the phagocytosis activity of macrophages, the cytokine and NO secretion ability of macrophages, and the expression levels of immune-function-related genes and proteins, and has an effect of inducing human PBMC cells to kill cancer cells.

The invention provides an exosome integrated antibacterial high polymer material and a preparation method thereof, and belongs to the field of intelligent biomimetic materials. The method comprises the following steps: introducing a benzimide bond into a molecular structure of a polylysine-chitosan copolymer to obtain a first copolymer; covalently coupling D-glutamic acid and electrostatically adsorbing lipoteichoic acid binding peptide to obtain a second copolymer; mixing the phosphate buffer solution of the aldehyde methyl cellulose with the phosphate buffer solution of the aminated chitosan to obtain a first mixture; adding a mixed solution of protocatechualdehyde and a FeCl3 solution into the first mixture to obtain a second mixture; under stirring, adding the second copolymer, the placenta mesenchymal stem cell exosome and Ag (at) rGO nanoparticles into the second mixture, and standing to obtain the exosome integrated antibacterial high polymer material. Therefore, the antibacterial property, the repairing property, the mechanical property and the environmental responsiveness of the exosome integrated antibacterial high polymer material are improved at the same time.

The invention discloses application of bacillus subtilis lipoteichoic acid in clostridium difficile infection, and relates to the technical field of biology. The invention discloses an application of bacillus subtilis lipoteichoic acid in preparation of a medicine for treating and / or preventing clostridium difficile infectious diseases. Experiments prove that the bacillus subtilis lipoteichoic acid can be combined with clostridium difficile toxins TcdA and TcdB so as to inhibit the cytotoxicity of the TcdA and TcdB, and the bacillus subtilis lipoteichoic acid can obviously reduce intestinal juice secretion induced by the clostridium difficile TcdA, so that the clostridium difficile TcdA can be used for inhibiting the clostridium difficile toxin TcdA and the clostridium difficile toxin TcdA and the clostridium difficile toxin TcdA; the bacillus subtilis lipoteichoic acid has a good effect in preventing or treating clostridium difficile infection, and a new thought is provided for developing a more effective, safer and more economical clostridium difficile treatment scheme.

The present application relates to the field of microorganism, discloses a lactobacillus paragasseri and application thereof. The lactobacillus paragasseri with the preservation number CGMCC No.36335 provided by the present application can metabolize multiple non-digestible carbohydrates, especially xylo-oligosaccharides, resistant starch, arabinose and mucin, and realize growth and reproduction by taking these substances as the only carbon source; can relieve constipation, and has the application prospect of preparing preparations for defecation; can produce high-yield lipoteichoic acid, and has the application value for industrial production of lipoteichoic acid.

This invention discloses the application of teichoic acid derived from Bifidobacterium animalis in the preparation of foods and pharmaceuticals that alleviate liver damage. The teichoic acid is extracted from Bifidobacterium animalis, including the following steps: extracting teichoic acid from Bifidobacterium animalis subsp. lactis YSJ-02. The Bifidobacterium animalis subsp. lactis YSJ-02 (… Bifidobacterium animalis subsp. lactis YSJ-02 is deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: M 20253030, address: Wuhan University, Wuhan, China. This total extract of teichoic acid can effectively alleviate liver damage caused by various factors.

The present application provides an exosome-integrated antimicrobial polymer material and its preparation method, belonging to the field of intelligent biomimetic materials. The method comprises: introducing a benzimide bond into the molecular structure of a polylysine-chitosan copolymer to obtain a first copolymer; covalently coupling D-glutamic acid and electrostatically adsorbing a lipoteichoic acid-binding peptide to obtain a second copolymer; mixing a phosphate buffer solution of aldehyded methylcellulose and a phosphate buffer solution of amino-modified chitosan to obtain a first mixture; adding a mixture of protocatechualdehyde and FeCl3 solution to the first mixture to obtain a second mixture; and adding the second copolymer, placental mesenchymal stem cell exosomes, and Ag@rGO nanoparticles to the second mixture under stirring, and allowing the mixture to stand to obtain the exosome-integrated antimicrobial polymer material. This method simultaneously improves the antimicrobial, repair, mechanical, and environmental responsiveness of the exosome-integrated antimicrobial polymer material.

The present disclosure relates to an antifungal composition using lipoteichoic acid derived from various Gram-positive bacteria to inhibit the adhesion and aggregation of Candida fungi, a pharmaceutical composition for preventing and treating candidiasis infection and the use thereof. Using this approach, since the effect of inhibiting adhesion and cohesion, which are the representative virulence factors of Candida fungal infection and candidiasis, has been confirmed, it can be used for antifungal use against Candida fungi and for prevention or treatment related to candidiasis infection.

The application belongs to the technical field of biological medicine and chemical synthesis, and particularly relates to a type II lipoteichoic acid and a preparation method thereof. The type II lipoteichoic acid is selected from a compound shown in the following formula I, wherein X is independently selected from H or a fatty acyl group. The application adopts a pre-activated O-glycosyl trichloroacetimidate donor glycosylation method to sequentially construct different types of alpha-configuration glycoside bonds in the molecule, and prepares a lipoteichoic acid skeleton unit through coupling of phosphate esters. The method has the advantages of high conversion rate, low cost, large-scale preparation and the like, and therefore has good practical application value.

Described are a number of aptamers that are specific to bind with peptidoglycan (“PGN”) with specificity over counter-targets lipopolysaccharides (“LPS”) and lipoteichoic acid (“LTA”), and associated methods.

The invention provides a non-ribosome polypeptide compound as well as a synthetic gene cluster BNP37 and application thereof, the structural sequence of the polypeptide compound is X0-X1-X2-X3-X4-X5-X6-X7-X8-X9-X10-X11, the polypeptide compound is a linear peptide or a cyclic peptide, and the cyclic peptide is a lactam cyclic peptide formed by connecting any one of X1, X3 and X5 with X11, or a lactam cyclic peptide or a lactone cyclic peptide formed by connecting X2 with X11. The compound disclosed by the invention can cause discharge of important ions in bacterial cells by targeting Gram-negative bacterium outer membrane lipid A and Gram-positive bacterium teichoic acid, so that bacterial death is caused. The compound disclosed by the invention has strong in-vivo and in-vitro antibacterial activity on a plurality of multi-drug-resistant gram-negative pathogenic bacteria such as escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, enterobacter cloacae and neisseria gonorrhoeae and a plurality of multi-drug-resistant gram-positive pathogenic bacteria such as staphylococcus aureus and enterococcus faecium.

The invention relates to the field of microorganisms, and discloses lactobacillus paragasseri and application thereof. The lactobacillus paraggaricus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.36335 provided by the invention can metabolize various non-digestible carbohydrates, especially xylooligosaccharide, resistant starch, arabinose and mucoprotein, and the substances are used as a unique carbon source to realize growth and reproduction; the composition can relieve constipation and has an application prospect of preparing a preparation for relaxing the bowels; the method can realize high yield of lipoteichoic acid, and has an application value for industrial production of lipoteichoic acid.

The present application belongs to the field of medicine and health, and relates to the use of lipoteichoic acid (LTA) and / or lipopolysaccharide (LPS) positive extracellular vesicles in plasma in the detection of bacterial inflammation and / or bacteremia. Specifically, the present application provides an in vitro method for detecting bacterial inflammation and / or bacteremia, comprising the following steps: providing a sample to be tested from a subject; isolating extracellular vesicles from the sample to be tested; detecting the level of LPS positive extracellular vesicles (LPS + EVs) and / or the level of LTA positive extracellular vesicles (LTA + EVs); if the levels of LPS + EVs and / or LTA + EVs increase, it indicates that the subject has bacterial inflammation and / or bacteremia.

The present disclosure relates to rifamycin analogs and antibody-drug conjugates thereof. The present disclosure relates to rifamycin analogue compounds, intermediates and precursors thereof, and pharmaceutical compositions capable of inhibiting bacterial growth (e.g., Staphylococcus aureus growth) and treating bacterial infections (e.g., Staphylococcus aureus infections). The present disclosure also relates to antibody-drug conjugates of rifamycin analogue compounds and antibodies specific for example to targets associated with infectious diseases, such as membrane glycoprotein receptor (MSR1), wall teichoic acid (WTA) or Protein A, and methods of using the antibody-drug conjugates to inhibit bacterial growth and treat bacterial infections.

The disclosure relates to rifamycin analog compounds, intermediates and precursors thereof, and pharmaceutical compositions capable of inhibiting bacterial growth (e.g., S. aureus growth) and treating bacterial infections (e.g., S. aureus infections). The disclosure further relates to antibody-drug conjugates of rifamycin analog compounds and antibodies, for example, antibodies specific for infectious disease-related targets such as membrane glycoprotein receptor (MSR1), wall teichoic acids (WTA) or Protein A, and methods of use thereof to inhibit bacterial growth and treat bacterial infections.

The present application relates to a kind of bacillus subtilis lipoteichoic acid and its extraction method and application, the extraction method of bacillus subtilis lipoteichoic acid includes: preparation chromatographic column, obtain packed chromatographic column standby;Bacillus subtilis bacterial liquid is added to n-butanol, after stirring uniformly, centrifugal, take middle lower layer water phase, freeze-drying is obtained by vacuum freeze-drying machine with lipoteichoic acid crude extract;The lipoteichoic acid crude extract is dissolved in the equilibrium buffer solution, after centrifugal processing, take supernatant, the supernatant is loaded into the packed chromatographic column, after the supernatant is all entered into the packed chromatographic column, eluent is added to the packed chromatographic column by peristaltic pump, filtrate is collected, and the bacillus subtilis lipoteichoic acid obtained after freeze-drying of the filtrate is obtained.The bacillus subtilis lipoteichoic acid is added to high soybean meal protein feed, can maintain the intestinal health of feeding high soybean meal protein feed bullfrog, improve the feeding effect, with excellent application prospect.

Virucidal and Microbicidal, Self-Disinfecting Resistive Hot Air Filter, and Methods of Manufacturing and Using the Same. The air filter includes Laser-Induced Graphene (LIG), which is a porous conductive graphene foam formed by photothermal conversion of a polyimide film (or a source of polymer or other LIG precursor material or another source) by a laser source. The LIG in the air filter can capture particulates and bacteria. Bacteria cannot proliferate even when immersed in a culture medium. Through a periodic Joule heating mechanism, the filter easily reaches temperatures above 300 °C. This destroys any microorganisms, including bacteria, and molecules that can cause adverse biological reactions and diseases, such as viruses, pyrogens, allergens, exotoxins, endotoxins, teichoic acids, mycotoxins, nucleic acids, and prions.

Disclosed are synthetic β-hairpin antimicrobial peptides (AMPs) of 13–15 amino acids and compositions thereof comprising a pathogen-recognition hairpin turn (e.g., LTA or VpPA) and β-sheet-forming side strands that self-assemble into nanonets and interwoven nanofibers upon contact with bacterial lipoteichoic acid or lipopolysaccharide, or fungal cell wall / membrane components. The peptides trap, immobilize, and kill bacteria and 0 fungi, including antibiotic-resistant strains, reduce motility, and neutralize endotoxin. Coated substrates and medical devices bearing covalently conjugated AMPs prevent or reduce biofilm formation, sustain antifouling activity, and attenuate inflammatory cytokines. Methods of treating infections and immune-related diseases, and kits comprising AMP-coated substrates or medical devices are also provided.

The invention discloses an immunohistochemical detection kit for bacteria in tumor cells as well as a use method and application of the immunohistochemical detection kit, and belongs to the technical field of tumor detection. According to the invention, lipoteichoic acid (LTA) and lipopolysaccharide (LPS) are taken as entry points, immunohistochemical detection is combined, and anti-LTA and anti-LPS antibodies are utilized to respectively detect the expression of gram-positive bacteria and gram-negative bacteria in tumor cells, so that the expression levels of LTA and LPS in breast cancer tissues are proved to be increased along with the increase of tumor malignancy degree, staging and metastatic potential for the first time; the potential risk of lymph node metastasis of tumor tissues with high expression of LTA and LPS is increased, and compared with negative lymph nodes, the expression of LTA / LPS is higher in positive metastatic lymph nodes; more importantly, the expression of LTA and LPS in breast cancer tumor tissues is related to OS (Overall Survival) and RFS (Reoccurrence-free Survival) of patients, so that LTA and LPS can be used as a marker for malignant evaluation of breast cancer and prediction of tumor metastasis, and is beneficial to stratification of high-risk and poor-prognosis patients.

The application belongs to the technical field of polymer material modification, and provides a chitosan grafted modified bio-based nylon material, a preparation method and application thereof.The preparation method comprises the following steps: mixing bio-based nylon salt, water, a catalyst and chitosan quaternary ammonium salt under a protective atmosphere, sequentially performing pre-polymerization and final polymerization to obtain the chitosan grafted modified bio-based nylon material.The chitosan quaternary ammonium salt is selected to react with the bio-based nylon salt, so that the number of positive charges on the surface of the original polyamide is increased, a large number of positive charges can promote the flocculation of the polyamide and the proteins, teichoic acid, lipopolysaccharide and the like with negative charges on the surface of the bacterial cell, and then the osmotic barrier effect of the cytoplasmic membrane is destroyed, the integrity of the bacterial cell is destroyed, and the nutrients are lost, so that the intrinsic antibacterial effect is achieved.The chitosan grafted modified bio-based nylon material has excellent antibacterial performance, mechanical properties and high-temperature resistance.

The present invention relates to a peptide that inhibits the activity of toll-like receptors (TLRs). The peptide according to the present invention can significantly reduce the expression of TNF-α in macrophages treated with lipoteichoic acid (LTA) or lipopolysaccharide (LPS), and thus has an excellent effect in preventing or treating inflammatory diseases, immune diseases, and cancer by effectively inhibiting the signaling pathway of TLRs, and significantly improves a skin antioxidant effect, moisture retention, elasticity, and thickness, thereby exhibiting an excellent skin improvement effect.

The invention relates to the technical field of medical instruments and microbiological detection, in particular to a blood culture bottle for enriching bacteria, which comprises a bottle body, a bottle cap, a culture medium, a magnetic bead and an anti-interference component, the bottle cap is detachably connected with the bottle body; the culture medium is a liquid culture medium, is positioned in the bottle body and is used for providing necessary nutritional ingredients for the growth of a biological material; the magnetic beads are magnetic particles, are arranged in the culture medium and are used for adsorbing and separating target strains; and the anti-interference assembly is arranged in the culture medium and is used for separating and adsorbing impurities in the culture medium. According to the method, negative charge components (such as lipopolysaccharide and teichoic acid) on the surfaces of bacteria are actively and directionally captured in the culture process through the electrostatic adsorption characteristic of the cationic magnetic beads, and an enrichment path independent of natural proliferation of the bacteria is constructed, so that the detection efficiency of bacteremia is improved, and the capturing efficiency of low-load bacteria in a complex sample is remarkably improved.

The application discloses a broad-spectrum antibacterial peptide SS-7 and application thereof, and belongs to the field of biological medicines. The amino acid sequence of the broad-spectrum antibacterial peptide SS-7 is VRRVRFVVLRRIKKWRRI, the antibacterial peptide SS-7 has a significant inhibiting effect on drug-resistant strains such as methicillin-resistant Staphylococcus aureus (ATCC43300) and various gram-positive bacteria and gram-negative bacteria, shows low toxicity and no hemolytic toxicity to eukaryotic cells and red blood cells of mammals, can combine LPS and lipophosphomannan on the surface of bacteria to achieve the effect of neutralizing bacteria, and has a good therapeutic protection effect on animals infected with bacteria. The antibacterial peptide SS-7 can be used for preparing antibacterial agents and drugs for preventing or treating bacterial infections, has the advantages of a wide antibacterial spectrum, a low drug resistance mutation frequency, low toxicity and a low production cost, can effectively cope with the current bacterial drug resistance problem, and has a wide application prospect and important clinical value.