12 results about "Lipoteichoic acid" patented technology

Provided are a postbiotic of Weizmannia coagulans, and a preparation method therefor, and a use thereof. The postbiotic comprises inactivated bacteria and / or lipoteichoic acid. The postbiotic can enhance the phagocytosis activity of macrophages, the cytokine and NO secretion ability of macrophages, and the expression levels of immune-function-related genes and proteins, and has an effect of inducing human PBMC cells to kill cancer cells.

The invention provides an exosome integrated antibacterial high polymer material and a preparation method thereof, and belongs to the field of intelligent biomimetic materials. The method comprises the following steps: introducing a benzimide bond into a molecular structure of a polylysine-chitosan copolymer to obtain a first copolymer; covalently coupling D-glutamic acid and electrostatically adsorbing lipoteichoic acid binding peptide to obtain a second copolymer; mixing the phosphate buffer solution of the aldehyde methyl cellulose with the phosphate buffer solution of the aminated chitosan to obtain a first mixture; adding a mixed solution of protocatechualdehyde and a FeCl3 solution into the first mixture to obtain a second mixture; under stirring, adding the second copolymer, the placenta mesenchymal stem cell exosome and Ag (at) rGO nanoparticles into the second mixture, and standing to obtain the exosome integrated antibacterial high polymer material. Therefore, the antibacterial property, the repairing property, the mechanical property and the environmental responsiveness of the exosome integrated antibacterial high polymer material are improved at the same time.

The present application relates to the field of microorganism, discloses a lactobacillus paragasseri and application thereof. The lactobacillus paragasseri with the preservation number CGMCC No.36335 provided by the present application can metabolize multiple non-digestible carbohydrates, especially xylo-oligosaccharides, resistant starch, arabinose and mucin, and realize growth and reproduction by taking these substances as the only carbon source; can relieve constipation, and has the application prospect of preparing preparations for defecation; can produce high-yield lipoteichoic acid, and has the application value for industrial production of lipoteichoic acid.

The present application provides an exosome-integrated antimicrobial polymer material and its preparation method, belonging to the field of intelligent biomimetic materials. The method comprises: introducing a benzimide bond into the molecular structure of a polylysine-chitosan copolymer to obtain a first copolymer; covalently coupling D-glutamic acid and electrostatically adsorbing a lipoteichoic acid-binding peptide to obtain a second copolymer; mixing a phosphate buffer solution of aldehyded methylcellulose and a phosphate buffer solution of amino-modified chitosan to obtain a first mixture; adding a mixture of protocatechualdehyde and FeCl3 solution to the first mixture to obtain a second mixture; and adding the second copolymer, placental mesenchymal stem cell exosomes, and Ag@rGO nanoparticles to the second mixture under stirring, and allowing the mixture to stand to obtain the exosome-integrated antimicrobial polymer material. This method simultaneously improves the antimicrobial, repair, mechanical, and environmental responsiveness of the exosome-integrated antimicrobial polymer material.

The present disclosure relates to an antifungal composition using lipoteichoic acid derived from various Gram-positive bacteria to inhibit the adhesion and aggregation of Candida fungi, a pharmaceutical composition for preventing and treating candidiasis infection and the use thereof. Using this approach, since the effect of inhibiting adhesion and cohesion, which are the representative virulence factors of Candida fungal infection and candidiasis, has been confirmed, it can be used for antifungal use against Candida fungi and for prevention or treatment related to candidiasis infection.

The application belongs to the technical field of biological medicine and chemical synthesis, and particularly relates to a type II lipoteichoic acid and a preparation method thereof. The type II lipoteichoic acid is selected from a compound shown in the following formula I, wherein X is independently selected from H or a fatty acyl group. The application adopts a pre-activated O-glycosyl trichloroacetimidate donor glycosylation method to sequentially construct different types of alpha-configuration glycoside bonds in the molecule, and prepares a lipoteichoic acid skeleton unit through coupling of phosphate esters. The method has the advantages of high conversion rate, low cost, large-scale preparation and the like, and therefore has good practical application value.

Described are a number of aptamers that are specific to bind with peptidoglycan (“PGN”) with specificity over counter-targets lipopolysaccharides (“LPS”) and lipoteichoic acid (“LTA”), and associated methods.

The invention relates to the field of microorganisms, and discloses lactobacillus paragasseri and application thereof. The lactobacillus paraggaricus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.36335 provided by the invention can metabolize various non-digestible carbohydrates, especially xylooligosaccharide, resistant starch, arabinose and mucoprotein, and the substances are used as a unique carbon source to realize growth and reproduction; the composition can relieve constipation and has an application prospect of preparing a preparation for relaxing the bowels; the method can realize high yield of lipoteichoic acid, and has an application value for industrial production of lipoteichoic acid.

The present application belongs to the field of medicine and health, and relates to the use of lipoteichoic acid (LTA) and / or lipopolysaccharide (LPS) positive extracellular vesicles in plasma in the detection of bacterial inflammation and / or bacteremia. Specifically, the present application provides an in vitro method for detecting bacterial inflammation and / or bacteremia, comprising the following steps: providing a sample to be tested from a subject; isolating extracellular vesicles from the sample to be tested; detecting the level of LPS positive extracellular vesicles (LPS + EVs) and / or the level of LTA positive extracellular vesicles (LTA + EVs); if the levels of LPS + EVs and / or LTA + EVs increase, it indicates that the subject has bacterial inflammation and / or bacteremia.

The present application relates to a kind of bacillus subtilis lipoteichoic acid and its extraction method and application, the extraction method of bacillus subtilis lipoteichoic acid includes: preparation chromatographic column, obtain packed chromatographic column standby;Bacillus subtilis bacterial liquid is added to n-butanol, after stirring uniformly, centrifugal, take middle lower layer water phase, freeze-drying is obtained by vacuum freeze-drying machine with lipoteichoic acid crude extract;The lipoteichoic acid crude extract is dissolved in the equilibrium buffer solution, after centrifugal processing, take supernatant, the supernatant is loaded into the packed chromatographic column, after the supernatant is all entered into the packed chromatographic column, eluent is added to the packed chromatographic column by peristaltic pump, filtrate is collected, and the bacillus subtilis lipoteichoic acid obtained after freeze-drying of the filtrate is obtained.The bacillus subtilis lipoteichoic acid is added to high soybean meal protein feed, can maintain the intestinal health of feeding high soybean meal protein feed bullfrog, improve the feeding effect, with excellent application prospect.

Disclosed are synthetic β-hairpin antimicrobial peptides (AMPs) of 13–15 amino acids and compositions thereof comprising a pathogen-recognition hairpin turn (e.g., LTA or VpPA) and β-sheet-forming side strands that self-assemble into nanonets and interwoven nanofibers upon contact with bacterial lipoteichoic acid or lipopolysaccharide, or fungal cell wall / membrane components. The peptides trap, immobilize, and kill bacteria and 0 fungi, including antibiotic-resistant strains, reduce motility, and neutralize endotoxin. Coated substrates and medical devices bearing covalently conjugated AMPs prevent or reduce biofilm formation, sustain antifouling activity, and attenuate inflammatory cytokines. Methods of treating infections and immune-related diseases, and kits comprising AMP-coated substrates or medical devices are also provided.

The present invention relates to a peptide that inhibits the activity of toll-like receptors (TLRs). The peptide according to the present invention can significantly reduce the expression of TNF-α in macrophages treated with lipoteichoic acid (LTA) or lipopolysaccharide (LPS), and thus has an excellent effect in preventing or treating inflammatory diseases, immune diseases, and cancer by effectively inhibiting the signaling pathway of TLRs, and significantly improves a skin antioxidant effect, moisture retention, elasticity, and thickness, thereby exhibiting an excellent skin improvement effect.