8 results about "Lipoteichoic acid" patented technology

Provided are a postbiotic of Weizmannia coagulans, and a preparation method therefor, and a use thereof. The postbiotic comprises inactivated bacteria and / or lipoteichoic acid. The postbiotic can enhance the phagocytosis activity of macrophages, the cytokine and NO secretion ability of macrophages, and the expression levels of immune-function-related genes and proteins, and has an effect of inducing human PBMC cells to kill cancer cells.

The present application relates to the field of microorganism, discloses a lactobacillus paragasseri and application thereof. The lactobacillus paragasseri with the preservation number CGMCC No.36335 provided by the present application can metabolize multiple non-digestible carbohydrates, especially xylo-oligosaccharides, resistant starch, arabinose and mucin, and realize growth and reproduction by taking these substances as the only carbon source; can relieve constipation, and has the application prospect of preparing preparations for defecation; can produce high-yield lipoteichoic acid, and has the application value for industrial production of lipoteichoic acid.

The application belongs to the technical field of biological medicine and chemical synthesis, and particularly relates to a type II lipoteichoic acid and a preparation method thereof. The type II lipoteichoic acid is selected from a compound shown in the following formula I, wherein X is independently selected from H or a fatty acyl group. The application adopts a pre-activated O-glycosyl trichloroacetimidate donor glycosylation method to sequentially construct different types of alpha-configuration glycoside bonds in the molecule, and prepares a lipoteichoic acid skeleton unit through coupling of phosphate esters. The method has the advantages of high conversion rate, low cost, large-scale preparation and the like, and therefore has good practical application value.

Described are a number of aptamers that are specific to bind with peptidoglycan (“PGN”) with specificity over counter-targets lipopolysaccharides (“LPS”) and lipoteichoic acid (“LTA”), and associated methods.

The invention relates to the field of microorganisms, and discloses lactobacillus paragasseri and application thereof. The lactobacillus paraggaricus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.36335 provided by the invention can metabolize various non-digestible carbohydrates, especially xylooligosaccharide, resistant starch, arabinose and mucoprotein, and the substances are used as a unique carbon source to realize growth and reproduction; the composition can relieve constipation and has an application prospect of preparing a preparation for relaxing the bowels; the method can realize high yield of lipoteichoic acid, and has an application value for industrial production of lipoteichoic acid.

The present application belongs to the field of medicine and health, and relates to the use of lipoteichoic acid (LTA) and / or lipopolysaccharide (LPS) positive extracellular vesicles in plasma in the detection of bacterial inflammation and / or bacteremia. Specifically, the present application provides an in vitro method for detecting bacterial inflammation and / or bacteremia, comprising the following steps: providing a sample to be tested from a subject; isolating extracellular vesicles from the sample to be tested; detecting the level of LPS positive extracellular vesicles (LPS + EVs) and / or the level of LTA positive extracellular vesicles (LTA + EVs); if the levels of LPS + EVs and / or LTA + EVs increase, it indicates that the subject has bacterial inflammation and / or bacteremia.

The present application relates to a kind of bacillus subtilis lipoteichoic acid and its extraction method and application, the extraction method of bacillus subtilis lipoteichoic acid includes: preparation chromatographic column, obtain packed chromatographic column standby;Bacillus subtilis bacterial liquid is added to n-butanol, after stirring uniformly, centrifugal, take middle lower layer water phase, freeze-drying is obtained by vacuum freeze-drying machine with lipoteichoic acid crude extract;The lipoteichoic acid crude extract is dissolved in the equilibrium buffer solution, after centrifugal processing, take supernatant, the supernatant is loaded into the packed chromatographic column, after the supernatant is all entered into the packed chromatographic column, eluent is added to the packed chromatographic column by peristaltic pump, filtrate is collected, and the bacillus subtilis lipoteichoic acid obtained after freeze-drying of the filtrate is obtained.The bacillus subtilis lipoteichoic acid is added to high soybean meal protein feed, can maintain the intestinal health of feeding high soybean meal protein feed bullfrog, improve the feeding effect, with excellent application prospect.

Disclosed are synthetic β-hairpin antimicrobial peptides (AMPs) of 13–15 amino acids and compositions thereof comprising a pathogen-recognition hairpin turn (e.g., LTA or VpPA) and β-sheet-forming side strands that self-assemble into nanonets and interwoven nanofibers upon contact with bacterial lipoteichoic acid or lipopolysaccharide, or fungal cell wall / membrane components. The peptides trap, immobilize, and kill bacteria and 0 fungi, including antibiotic-resistant strains, reduce motility, and neutralize endotoxin. Coated substrates and medical devices bearing covalently conjugated AMPs prevent or reduce biofilm formation, sustain antifouling activity, and attenuate inflammatory cytokines. Methods of treating infections and immune-related diseases, and kits comprising AMP-coated substrates or medical devices are also provided.