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Application of lipoteichoic acid from clostridium butyricum in adjusting immune response of aquatic livestock

A technology of lipoteichoic acid and aquatic animals, which is applied in the direction of bacterial medical raw materials, drug combinations, allergic diseases, etc., can solve the problems of long-term colonization of probiotics, low survival rate, and inability to fully exert immune regulation, etc., to achieve The effect of good market prospects

Inactive Publication Date: 2014-11-26
NINGBO INST OF TECH ZHEJIANG UNIV ZHEJIANG
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  • Abstract
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Problems solved by technology

However, there are also many limitations in the use of probiotics in the production of aquatic animals: (1) probiotics have high environmental requirements, and the aquaculture water cannot meet the needs of their growth and proliferation, resulting in low survival rates and poor effects; ( 2) Probiotics cannot colonize the intestinal tract of aquatic animals for a long time, and it is difficult to exert antibacterial effect for a long time; (3) The density of probiotics in the intestinal tract of aquatic animals is too low to fully exert the immune regulation function, and the inhibitory effect on pathogenic bacteria is weak

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  • Application of lipoteichoic acid from clostridium butyricum in adjusting immune response of aquatic livestock
  • Application of lipoteichoic acid from clostridium butyricum in adjusting immune response of aquatic livestock
  • Application of lipoteichoic acid from clostridium butyricum in adjusting immune response of aquatic livestock

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Embodiment Construction

[0029] Further describe the present invention in detail below by embodiment, but the present invention is not limited only to following embodiment.

[0030] 1. Extraction and preparation method of lipoteichoic acid

[0031]Prepare 2% TX-114 with 50mmol / L, pH 6.5 Tris buffer solution plus TX-114, store in refrigerator at 4°C for later use; take 10ml of the cultured bacteria solution for 48h respectively in several plastic centrifuge tubes of known quality , centrifuge at 5000r / min at room temperature for 30min, weigh each tube after removing the upper culture solution, and calculate the mass of wet bacteria; suspend 1g of wet bacteria in pre-cooled 10ml 2% TX-114 solution, stir and lyse the bacteria with a magnetic stirrer 30min, overnight at 4°C; centrifuge at 5000r / min at 4°C for 30min, remove cell debris, and collect the upper extract; bath the extract in water at 50°C for 30min, centrifuge at 5000r / min at room temperature for 30min, separate the extract into two layers, and...

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Abstract

The invention discloses an application of lipoteichoic acid from clostridium butyricum in adjusting an immune response of aquatic livestock. The lipoteichoic acid is from clostridium butyricum and is different from cell wall lipoteichoic acid structures of other gram-positive bacteria. The lipoteichoic acid can improve intestinal immune functions of the aquatic livestock, suppress infections of pathogenic bacteria to the aquatic livestock and reduce incidence of diseases.

Description

Technical field: [0001] The present invention relates to Clostridium butyricum cell wall lipoteichoic acid for regulating the immune response of aquatic animals, specifically the use of the lipoteichoic acid from Clostridium butyricum in regulating the immune response of aquatic animals. Background technique: [0002] Bacterial disease is the main infectious disease in aquaculture industry, which seriously threatens the health of aquaculture animals. At present, the use of high doses of antibacterial drugs is still the main way to prevent and control fish bacterial diseases. High doses of antibacterial drugs will cause drug residues in aquatic products, endangering the health of consumers. At the same time, long-term use of antibacterial drugs can also cause bacterial resistance, which in turn makes bacterial diseases more difficult to prevent and control. In this context, screening probiotic strains and using the probiotic effect of bacteria to prevent and control pathoge...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P37/02
Inventor 王进波齐莉莉梅乐和周云晓
Owner NINGBO INST OF TECH ZHEJIANG UNIV ZHEJIANG
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