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Postbiotics as well as preparation method and application thereof

A technology of epigenetics and strains, applied in biochemical equipment and methods, methods based on microorganisms, bacteria used in food preparation, etc., can solve the problems of unclear functions of postbiotics, poor stability and dispersion, unstable product performance, etc. problem, to achieve the effect of shortening the production cycle, good thermal stability, and shortening the cycle

Pending Publication Date: 2022-05-13
SICHUAN GAOFUJI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, at present, domestic epigenetics mainly rely on imports from Japan, Europe and the United States, and the functions of existing postbiotics are not clear, lack of effective components, unstable product quality, darker color, peculiar smell, especially poor stability and dispersion, and cannot be used on a large scale. Therefore, there is an urgent need to develop postbiotics with clear components and functions that can be produced on an industrial scale, and combining the characteristics of postbiotics to explore industrial preparation methods has an urgent technical demand
[0004] In addition, most of the existing postbiotics are mixed with various strains or other components, such as a compound postbiotic solid drink disclosed in CN113854461A and its preparation method, and the postbiotic milk tablet disclosed in CN112544721A and its preparation method. The preparation of postbiotics involving a variety of probiotics is complex, and the product performance is unstable. In addition, there are few reports in the prior art of postbiotics prepared by fermentation of a single strain and capable of enhancing immunity. Therefore, it is urgent to develop new probiotics. epigenetic

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  • Postbiotics as well as preparation method and application thereof
  • Postbiotics as well as preparation method and application thereof
  • Postbiotics as well as preparation method and application thereof

Examples

Experimental program
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Embodiment 1

[0064] refer to Figure 1-10 , a postbiotic of the present embodiment, said postbiotic includes inactivated Lactobacillus plantarum LP220 and its fermentation metabolites, and the preservation number of said Lactobacillus plantarum LP220 is CCTCC NO: M2018465.

[0065] The composition of the postbiotics includes: Lactobacillus plantarum LP220 is 1.2*10 11 CFU / g (number of bacteria ≥ 1*10 11 CFU / g), the content of lipoteichoic acid is 36.2ng / kg, the total content of short-chain fatty acids is 99.97μg / g, the content of polypeptide is 6.0%, and the small molecule peptide with molecular weight <1000DA accounts for 89.1% of the total peptide content , the organic acid content is 127mg / g.

[0066] The preparation method of a kind of postbiotic of the present embodiment comprises the following steps:

[0067] S1, strain activation

[0068] Sterilization of all used utensils: need to be placed in a pressure steam sterilizer, sterilized at 121°C for 25-30 minutes before use.

[00...

Embodiment 2

[0167] A postbiotic of this embodiment, said postbiotic includes inactivated Lactobacillus plantarum LP220 and its fermentation metabolites, and the preservation number of said Lactobacillus plantarum LP220 is CCTCC NO: M2018465. The composition of the postbiotics includes: the number of Lactobacillus plantarum LP220 is 2*10 11 CFU / g, the content of lipoteichoic acid is 35.7ng / kg, the content of short-chain fatty acid is 96.12μg / g, the content of polypeptide is 6.25%, and the small molecule peptide with molecular weight <1000DA accounts for 88.4% of the total peptide content, organic acid The content is 116mg / g.

[0168] The preparation method of a kind of postbiotic of this embodiment, and the preparation method of embodiment 1, there is following difference:

[0169] S2, fermentation and inactivation

[0170]Transfer about 15,000ml of the third-generation activated culture strain to a cleaned and sterilized fermenter according to the inoculum size of 6.0% (V / V). The temper...

Embodiment 3

[0174] A postbiotic of this embodiment, said postbiotic includes inactivated Lactobacillus plantarum LP220 and its fermentation metabolites, and the preservation number of said Lactobacillus plantarum LP220 is CCTCC NO: M2018465. The composition of the postbiotics includes: the number of Lactobacillus plantarum LP220 is 1.5*10 11 CFU / g, the content of lipoteichoic acid is 35.1ng / kg, the content of short-chain fatty acid is 93.12μg / g, the content of polypeptide is 6.62%, and the small molecule peptide with molecular weight <1000DA accounts for 85.8% of the total peptide content, organic acid The content is 110mg / g.

[0175] The preparation method of a kind of postbiotic of this embodiment, and the preparation method of embodiment 1, there is following difference:

[0176] S2, fermentation and inactivation

[0177] Transfer about 20,000ml of the third-generation activated culture strain to a cleaned and sterilized fermenter according to the inoculum size of 5% (V / V). In the c...

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Abstract

The invention relates to a metagen and a preparation method and application thereof, the metagen comprises inactivated lactobacillus plantarum LP220 and a fermentation metabolite thereof, and the preservation number of the lactobacillus plantarum LP220 is CCTCC (China Center For Type Culture Collection) NO: M2018465. The metagen comprises the following components: lactobacillus plantarum LP220 greater than or equal to 1 * 10 < 11 > CFU / g, lipoteichoic acid greater than or equal to 35 ng / kg, short-chain fatty acid greater than or equal to 90 [mu] g / g, polypeptide content greater than or equal to 6%, and molecular weight 1t; 1000DA small molecule peptide accounts for more than 80% of the total peptide content, and the organic acid content is more than or equal to 100mg / g. The invention further discloses a preparation method of the postbiotics and application of the postbiotics in preparation of dietary supplements, health care products, medicines, foods or solid beverages for enhancing immunity. The metagen contains lactobacillus plantarum thalli above 1 * 10 < 11 > CFU / g, is rich in functional components such as small molecule peptides, short-chain fatty acids, organic acids, amino acids and the like, and improves and regulates the organ index, cellular immune function and NK cell activity of a body to remarkably enhance the immune function of the body.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to a postbiotic and its preparation method and application. Background technique [0002] Postbiotics refer to preparations of non-living microorganisms and / or their components that are beneficial to the health of the host. Postbiotics are artificially inactivated microbial cells. Metabolites or cell components that have been proven to be beneficial to health can be added, or not. According to classification, postbiotics mainly include metabolites and bacterial components. Metabolites include: Organic acids, short-chain fatty acids, intracellular polysaccharides, vitamins, proteins, enzymes, lipids; bacteria components include: lipoteichoic acid, teichoic acid, peptidoglycan, cell surface proteins, polysaccharides, cell membrane proteins, extracellular polysaccharides. Compared with probiotic powder, postbiotics are more convenient to transport and store, and the use ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02A23L33/135A23L2/52A61K35/747A61P37/04C12R1/25
CPCC12N1/20C12N1/02C12N1/005A23L33/135A23L2/52A61K35/747A61P37/04A23V2002/00A23V2400/169A23V2200/324Y02A50/30
Inventor 蒲小平舒梨邓燕钟综谢建将景晓青卫娟
Owner SICHUAN GAOFUJI BIOLOGICAL TECH
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