31results about How to "Good pH tolerance" patented technology

The invention discloses a method for treating phenol-containing waste water by sawdust immobilized alcaligenes faecalis. The method has the advantages that (1) the immobilized alcaligenes faecalis is easy to culture and has the advantages of simple immobilization process, excellent catalytic degradation effect, resistance to high-concentration phenol-containing waste water with the concentration of phenol up to 3000mg.milligram<-1>, strong phenol decomposition capability and repeated use; (2) the carrier of the immobilized alcaligenes faecalis is sawdust, and modified utilization of the sawdust is realized by a chemical method, so the method improves the treatment efficiency for the phenol-containing waste water by means of microbial degradation, and has a broad development prospect; (3) the immobilized alcaligenes faecalis prepared through taking sawdust as the carrier has excellent elasticity, strong resistance to temperature and pH value, stable degradation property and long half life period; and (4) when used for treating the phenol-containing waste water, the sawdust immobilized alcaligenes faecalis has the advantages of simple process, low running cost, moderate conditions, environmental protection, uneasy loss of thalli, high concentration of microorganism inside the carrier, quick degradation speed, high degradation efficiency and no secondary pollution, and can realize continuous industrial degradation of the phenol-containing waste water at the same time.

The invention belongs to the technical field of microorganism gene engineering, and particularly relates to deacetylase capable of producing high-yield glucosamine and a coding gene thereof. The gene CsnagA is separated from the natural strain cronobacter sakazakii 0360. Genomic DNA is separated from cronobacter sakazakii with the preservation number of CCTCC NO:M 2016162. The nucleotide sequence of the gene is shown in SEQ ID NO:1. The sequence of protein coded by the gene is shown in SEQ ID NO:2. By means of genetic recombination, the gene is transferred into escherichia coli to construct genetically engineered bacteria, so as to obtain an efficient glucosamine fermentation production capacity, and glucosamine can be produced with the enzymatic biosynthesis method. Compared with the prior art, the deacetylase has the advantages that the production efficiency is improved, and the production cost is reduced.

The invention discloses thermophilic recombinant II type pullulanase and application thereof, and belongs to the technical field of gene engineering. The thermophilic recombinant II type pullulanase is obtained through heterologous expression of II type pullulanase in escherichia coli, the most proper pH is 6.6, the thermophilic recombinant II type pullulanase has good pH tolerance under the condition that the pH value is 5.8-8.0, the most proper temperature is 95 DEG C, heat preservation is carried out for 10 h at 95 DEG C, the remaining enzyme activity is larger than 50%, and a relatively high specific activity can be shown under strong reduction conditions; if DTT is added into a culture environment, the specific activity of Sumo-PulPy can be increased by 237.2%. The invention also provides a combined truncated mutant delta28N+delta791C of the II type pullulanase Sumo-PulPy. The specific activity of the enzyme mutant is 32.18+/-0.92 U/mg and is 5.99 times that of wild enzyme, and the mutant has important industrial application value and potential.

The invention discloses an immobilization method of sucrose isomerase, and belongs to the technical field of bioengineering. According to the method, recombinant bacillus brevis is taken as production strain to produce the sucrose isomerase; then immobilization is performed; the enzyme activity is 35.2 U/g after immobilization; the enzymatic activity recovery reaches 70.3 percent; the optimum temperature of immobilized enzyme is 40 DEG C (30 DEG C for free enzyme); and the optimum pH is 4.5 (6.0 for free enzyme); the fact indicates that the immobilized enzyme has better temperature and pH tolerance. Sucrose concentration at 600g*L <1> is taken as a substrate, and the maximum product yield can reach 87.8 percent. After conversion is continuously performed for 16 times under the optimum conversion condition, the product yield is still 87.52 percent, and the fact indicates that the immobilized enzyme has good operation stability and high isomaltulose synthesis capability. The immobilization method is simple to operate and low in cost, and provides a reference for industrial application.

The invention discloses a cationic flocculant and a preparation method thereof. The cationic flocculant is polymerized from 10-15 parts by weight of melamine, 40-65 parts by weight of glyoxal, 5-10 parts by weight of catalyst, 0.1-0.5 part by weight of polymerization inhibitor and 30-60 parts by weight of water. The cationic flocculant provided by the invention has the advantages of good stability and excellent resistance to acids and bases; and the cationic flocculant is used for substituting for the melamine resin type flocculants used at present such as polyacrylamide (PAM), dimethyl diallyl ammonium chloride, melamine-formaldehyde, dicyandiamide-formaldehyde condensation polymer and the like; therefore, an excellent flocculating settling effect is achieved while the dosage is reduced.

The invention discloses a preparation method of raw starch-digesting glucoamylase and application of the raw starch-digesting glucoamylase to raw starch hydrolysis and preparation of ethanol by simultaneous saccharification and fermentation of raw starch. The raw starch-digesting glucoamylase is obtained after codon optimization, and a nucleotide sequence of the raw starch-digesting glucoamylase is shown as any of from A1) to A5): A1), sequence 3 in a sequence table; A2), nucleotides from 1st position to 1878th position in the sequence 3 in the sequence table; A3), DNA molecules shown by nucleotides in 19th position to 1878th position in the sequence 3 in the sequence table; A4), DNA molecules shown by nucleotides in 19th position to 1857th position in the sequence 3 in the sequence table; A5), a protein sequence shown in sequence 4 in a coding sequence table and showing 98% identity with the sequence limited by A1), A2), A3) or A4). Through experiments, it is proven that raw starch-digesting glucoamylase genes can be effectively expressed in a heterologous expression system to obtain the raw starch-digesting glucoamylase rPoGA15A, and the raw starch-digesting glucoamylase rPoGA15A and alpha-amylase act synergistically to hydrolyze the raw starch rapidly and efficiently and prepare the ethanol by simultaneous saccharification and fermentation of the raw starch.

The invention belongs to the technical field of biological engineering, and relates to a new saccharomyces cerevisiae strain mutant with high yield of glutathione and the class name of Saccharomyces cerevisiae Y518, and application of Gamma-glutamyl cysteine synthetase gene and glutathione synthetase gene that are generated by the strain, and the strain application for glutathione preparation. An original NTG saccharomyces cerevisiae strain with continuous induction is inductively processed for 4 times, thus obtaining the saccharomyces cerevisiae strain mutant Y518. As Gamma-glutamyl cysteine synthetase and glutathione synthetase that are generated by the saccharomyces cerevisiae strain Y518 experience point mutation, compared with an original strain, the saccharomyces cerevisiae Y518 has stronger acid resistance, stronger pH tolerance, good stability and remarkably raised yield of glutathione. The glutathione preparation that utilizes the saccharomyces cerevisiae Y518 is characterized by simple operation, stability, good repeatability, high yield and the like, generates stable and cheap glutathione, and is favorable for scaled glutathione production.

The invention discloses a preparation method of lanthanum hydroxide modified biochar, belongs to the field of functional materials and environmental water treatment, and is used for removing phosphatein municipal sewage. According to the method, sycamore fruit balls are used as a biochar raw material, and after lanthanum modification, the efficient, economical and environment-friendly adsorbent La-TC is prepared. Aiming at the concentration of phosphorus in municipal sewage of 4-10mg/L, when the using amount of La-TC is 0.3-0.5 g/L, the pH value of the solution is 3-9 and the temperature is 25-40 DEG C, 100% removal of phosphate can be realized within 15-20 minutes. Meanwhile, high competitive anion interference resistance is achieved, and the adsorption rate of La-TC can still reach 100%after three times of adsorption and desorption cycles. In an experiment of removing municipal sewage by using a fixed bed column, the maximum bed volume of 0.5-1.0 g of La-TC component treatment is 420BV-480BV at a flow rate of 1mL/min, which indicates that La-TC has a wide prospect in practical engineering application.

The invention discloses a novel aerogel heat insulation film. The heat insulation film comprises a heat-insulating film body, a base layer is arranged in the middle of the heat insulation film body, aphase change layer, an outer aerogel layer and a protective layer are sequentially arranged on the upper side of the base layer, an inner aerogel layer and a release film layer are sequentially arranged on the lower side of the base layer, the outer aerogel layer, the phase change layer, the base layer inner aerogel layer and the release film layer are sequentially bonded through bonding layers,silicon dioxide aerogel serves as a heat insulation main body material, polydimethylsiloxane serves as a protection material, a novel heat insulation material which has certain mechanical strength, flexibility, corrosion resistance, moisture resistance and environmental protection is formed, and through a composite phase change material, heat storage is achieved, and constant-temperature environment simulation is achieved. Compared with a traditional aerogel heat insulation film, the novel heat insulation film is better in tolerance to humidity and pH value, and the powder falling condition does not exist. Therefore, the novel heat insulation film provided by the invention has a great prospect in the field of heat insulation.

The invention discloses a pyrazinamide hydrolase, and an encoding gene and an application thereof. The hydrolase has an amino acid sequence represented as the SEQ ID No.1; and the encoding gene is a DNA molecule which has a nucleotide sequence represented as the SEQ ID No.2. The pyrazinamide hydrolase is high in enzyme activity and has good pH tolerance. The invention demonstrates a direction of therapy on patients, which has pyrazinamide resistance in vivo, by combining pyrazinamide with the pyrazinamide hydrolase.

The invention discloses immobilized lipase and a preparation method thereof. The preparation method comprises the following steps: dissolving lipase enzyme powder by utilizing a potassium phosphate buffering solution with the concentration of 1.0mol/L and the pH (Potential of Hydrogen) of 6.0, so as to prepare an enzyme solution with the concentration of 2.0mg/mL; after centrifuging at the speed of 10000r/min, obtaining a clarified enzyme solution; putting epoxy resin with the model of LXEP-120 into the enzyme solution according to the ratio of 5.0g to 4mL; immobilizing for 12h under the conditions of 150rpm and 22 DEG C; then washing for 3 times by utilizing the potassium phosphate buffering solution with the concentration of 1.0mol/L and the pH of 6.0; filtering and drying at 40 DEG C toobtain the immobilized lipase, wherein the enzyme activity is 400U/g and the enzyme activity recovery rate reaches 92.79 percent. The immobilized lipase has good pH tolerance, temperature tolerance,operation stability and storage stability and meets the requirements of industrial application.

The invention belongs to the technical field of microorganisms, and discloses an enterococcus faecium DK-01 and an application thereof. The enterococcus faecium can increase the number of lactic acid bacteria in intestinal flora, has a positive regulation effect on the structure of the intestinal flora, improves the immune function of the organism, reduces the incidence rate of diseases, meanwhile, can improve the digestion and absorption efficiency of female livestock on nutrient substances in feed, improves the pregnancy rate, pregnancy rate and lactation ability of the female livestock, and improves the reproductive performance of the female livestock.

The invention discloses a method for catalytically treating organic matters in high-salinity wastewater by using waste warmer pastes, which comprises the following steps: taking out powder substances in the waste warmer pastes, washing with water, and drying at 50-70 DEG C to obtain a heterogeneous photo-Fenton catalyst; adding the catalyst obtained in the step 1 into to-be-treated organic matter-containing high-salinity wastewater, and uniformly stirring and dispersing to obtain dispersion liquid; adding an oxidizing agent into the obtained dispersion liquid, and starting a degradation reaction by taking a 20-300W xenon lamp as a light source; according to the invention, the waste warmer paste is prepared into the heterogeneous photo-Fenton catalyst and is applied to environmental pollutant treatment, so that the effect of treating waste with waste is achieved; and meanwhile, refractory organic matters in the high-salinity wastewater can be efficiently treated, good pH tolerance and excellent cyclic catalysis performance are achieved, and a new thought is provided for actual treatment of organic pollutants in the high-salinity wastewater.

The invention discloses a salmonella phage (salmonella sp. Phage) JNwz02 and application of the salmonella phage JNwz02. The strain is preserved in Guangdong Microbial Culture Collection Center on May 12, 2021, and the preservation number is GDMCC No: 61662-B1. The bacteriophage is a virulent bacteriophage, can be used for cracking salmonella of eight serotypes such as stetank vil, hirgania and typhoid, including multiple strains of drug-resistant salmonella, and also can be used for cracking enterohemorrhagic Escherichia coli O157: H7. The invention also discloses an application of the bacteriophage JNwz02 as a bacteriostatic agent in food storage.

The invention discloses a method used for producing acidic cellulase via solid fermentation of forestry and agricultural residues with inonotus obliquus. The method comprises steps of 1, bacterial strain activation, 2, liquid strain culturing; 3, solid fermentation; and 4, acidic cellulase separation. The method is low in equipment investment, simple in post-treatment, and low in cost; almost no pollution is caused; and produced cellulase possesses excellent pH tolerance and temperature stability.