A low-temperature alkaline esterase estsl3 resistant to salt and organic solvents and its gene and application

A technology resistant to organic solvents and alkalinity, applied in the field of genetic engineering, can solve problems such as inability to apply, and achieve the effects of increasing the yield of the target product, reducing energy consumption, and improving flavor
CN105176943BActive Publication Date: 2018-09-18FUZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
FUZHOU UNIV
Publication Date
2018-09-18

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Abstract

The invention relates to the field of genetic engineering, and provides a salt-tolerant and organic solvent-tolerant low-temperature alkaline esterase EstSL3, a genome coding gene estSL3 and a recombinant vector and a recombination strain comprising the gene, the esterase EstSL3 comes from halophilic and basophilic bacteria, the amino acid sequence of the esterase EstSL3 is shown as SEQ ID NO.1, and the nucleotide sequence of the genome coding gene estSL3 of the esterase is shown as SEQ ID NO.2. The esterase EstSL3 is adapted to pH9.0 and the reaction temperature of 30DEG C best, and the esterase EstSL3 still has about 70 percent of enzyme activity under 0DEG C, which indicates that the enzyme is a low-temperature esterase. Under the existence of 0.5 to 4.0M NaCl, more than 98 percent of enzyme activity remains, and moreover, under the concentration of 4M and 5M NaCl, the esterase EstSL3 is highly stable. The esterase disclosed by the invention has the characteristics of low temperature, alkalinity, salt tolerance, detergent tolerance and organic solvent tolerance, and can be applied in industrial fields such as fine chemical, pharmacy, detergents and food.
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Description

technical field

[0001] The invention relates to the field of genetic engineering, in particular, the invention relates to a low-temperature alkaline esterase EstSL3 resistant to salt and organic solvents and its gene and application. Background technique

[0002] Esterase (Esterase E.C.3.1.1.1) is a general term for enzymes that catalyze the hydrolysis and synthesis of ester bonds. During hydrolysis, it catalyzes ester bonds to produce alcohols and carboxylic acids, and during synthesis, it dehydrates and condenses carboxyl groups of acids and hydroxyl groups of alcohols to form esters and other fragrance products. During the catalytic reaction process of esterase, it has high catalytic specificity for substrate, its reaction conditions are mild, its by-products are few, and it does not require coenzyme, so it has high application value in industrial production and environmental treatment.

[0003] Esterase-producing organisms are widely distributed in nature, ranging from ...

Claims

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