Method used for producing acidic cellulase via solid fermentation of forestry and agricultural residues with inonotus obliquus

Abstract

The invention discloses a method used for producing acidic cellulase via solid fermentation of forestry and agricultural residues with inonotus obliquus. The method comprises steps of 1, bacterial strain activation, 2, liquid strain culturing; 3, solid fermentation; and 4, acidic cellulase separation. The method is low in equipment investment, simple in post-treatment, and low in cost; almost no pollution is caused; and produced cellulase possesses excellent pH tolerance and temperature stability.

Description

technical field [0001] The invention relates to the technical field of fermentation engineering, in particular to a process for producing acid cellulase by using Inonotus obliquus for solid fermentation of agricultural and forestry waste. Background technique [0002] Plant fibers are ubiquitous in nature, and most plant fibers are discarded because they cannot be decomposed and utilized. If these celluloses can be used rationally, they can not only benefit human beings as new resources and new energy sources, but also alleviate or solve the problems of crop resources. The problem of environmental pollution is of great strategic significance. [0003] Cellulose molecules are chain polymers formed by β-2D glucose molecules combined with β-1,4 glycosidic bonds, so it is difficult to decompose. Cellulase can finally hydrolyze cellulose into glucose. However, cellulase is generally only produced in microorganisms, and a small part of animals can also produce cellulase. At pre...

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Problems solved by technology

[0004] In the process of microbial fermentation, the low enzyme production ability and unstable quality of cellulase-producing bacteria are the main bottlenecks in the research and practical application of cellulase at present. How to improve the technology of cellulase activity in a targeted manner needs to be improved: Current research channels show that cellulase can be expressed in other engineered bacteria, but how to develop a set that can express cellulase with good thermostability, low end product inhibition regulation and high yield The research plan is the problem that researchers are now discussing: especially now that my country's cellulase still relies heavily on foreign imports, its price is very expensive, which greatly limits its application range, the production of localized cellulase and the reduction of cellulase The production cost is still an urgent problem to be solved

So far, there is no report about the production of cellulase by solid-state fermentation of the white-rot fungus Inonotus obliquus

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