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Method for treating phenol-containing wastewater using wood chip immobilized Alcaligenes faecalis

A technology of Alcaligenes faecalis and sawdust, which is applied in the field of microbial catalytic degradation of phenol-containing wastewater, can solve the problems of low microbial concentration, large floor area, and slow degradation speed, and achieve high microbial concentration, low operating costs, and fast degradation speed Effect

Inactive Publication Date: 2010-09-15
XIANGTAN UNIV
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  • Summary
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  • Application Information

AI Technical Summary

Problems solved by technology

However, there are disadvantages such as easy loss of bacteria, low microbial concentration, slow degradation rate, secondary pollution caused by a large amount of sludge, and large floor area.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Put 5.0kg of 40-mesh sawdust into 15% sodium sulfite solution, adjust the pH to 4.0, soak at 60°C for 48h, and rinse with water. Neutralize with 20% sodium hydroxide solution, wash with water, and dry.

[0017] A 100L container was connected to Alcaligenes faecalis JH1013 for routine culture, the sawdust was soaked in the bacteria liquid, the temperature of the bacteria liquid was 30 ℃, pH 7.0, and the bacteria liquid was immobilized for 48 hours, and the bacteria liquid was filtered out. Sawdust immobilized Alcaligenes faecalis.

[0018] The sawdust-immobilized Alcaligenes faecalis was filled into the cylindrical reactor in the tower biological filter, fresh culture solution was injected, and the sawdust-immobilized Alcaligenes faecalis was activated for 18 hours, and the residual culture solution was washed with water. Into a chemical plant containing phenol wastewater (the average COD of the inlet is 4000mg·L -1 , The phenol concentration at the inlet is 990mg·L -1 ), th...

Embodiment 2

[0020] Put 5.0kg of 80 mesh sawdust into 20% sodium sulfite solution, adjust the pH to 4.0, soak at 60°C for 48h, rinse with water, neutralize with 15% sodium hydroxide solution, wash with water, and dry.

[0021] A 100L container was connected to Alcaligenes faecalis JH1013 for routine culture, the sawdust was soaked in the bacteria liquid, the temperature of the bacteria liquid was 35 ℃, pH 7.5, and the bacteria liquid was immobilized for 72 hours, and the bacteria liquid was filtered out. Sawdust immobilizes Alcaligenes faecalis.

[0022] The immobilized Alcaligenes faecalis is filled into the cylindrical reactor in the tower biological filter, fresh culture solution is injected, the wood chips are activated to immobilize Alcaligenes faecalis for 48 hours, and the residual culture solution is washed with water. Enter phenol-containing wastewater from a coking plant (average COD at the inlet is 5000 mg·L -1 , The phenol concentration at the inlet is 1500mg·L -1 ), the wastewater ...

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Abstract

The invention discloses a method for treating phenol-containing waste water by sawdust immobilized alcaligenes faecalis. The method has the advantages that (1) the immobilized alcaligenes faecalis is easy to culture and has the advantages of simple immobilization process, excellent catalytic degradation effect, resistance to high-concentration phenol-containing waste water with the concentration of phenol up to 3000mg.milligram<-1>, strong phenol decomposition capability and repeated use; (2) the carrier of the immobilized alcaligenes faecalis is sawdust, and modified utilization of the sawdust is realized by a chemical method, so the method improves the treatment efficiency for the phenol-containing waste water by means of microbial degradation, and has a broad development prospect; (3) the immobilized alcaligenes faecalis prepared through taking sawdust as the carrier has excellent elasticity, strong resistance to temperature and pH value, stable degradation property and long half life period; and (4) when used for treating the phenol-containing waste water, the sawdust immobilized alcaligenes faecalis has the advantages of simple process, low running cost, moderate conditions, environmental protection, uneasy loss of thalli, high concentration of microorganism inside the carrier, quick degradation speed, high degradation efficiency and no secondary pollution, and can realize continuous industrial degradation of the phenol-containing waste water at the same time.

Description

Technical field [0001] The invention relates to a method for catalytically degrading phenol-containing wastewater by microorganisms. Background technique [0002] Phenol-containing wastewater is a source of water pollution with wide sources, large quantities, high concentrations, and great environmental hazards. It is also one of the key difficulties that my country's water pollution control needs to solve urgently. Biodegradation of phenol-containing wastewater has the advantages of simple process, mild conditions, environmental friendliness, low treatment cost, and low secondary pollution. Generally 5-500mg·L -1 The phenol-containing wastewater is suitable for biological treatment, but the treatment effect for higher-content phenol-containing wastewater is not ideal [Sun Min et al., Chinese Journal of Medical Engineering. 2004, 12(3): 93-94; Zhang Jie et al., Sichuan Environment. 2006, 25(1): 8-10]. Free microorganisms can degrade high-concentration phenol-containing wastewat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C12N11/00
Inventor 曾虹燕姜和夏葵廖凯波黄炎王亚举
Owner XIANGTAN UNIV
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