212 results about "Alcaligenes faecalis" patented technology

The invention discloses a microbial inoculum for decomposing lignocellulose and application thereof. The active component of the microbial inoculum consists of Alcaligenes faecalis, Bacteroides xylanisolvens, Clostridium xylanolyticum, Clostridium lentocellum, Flavobacterium aquatile, and Pseudomonas stutzeri. Experiments prove that the microbial inoculum has high degradation rate to the lignocellulose, and the degradation rate can reach between 60 and 70 percent. The microbial inoculum has low cost and simple preparation, breaks through the limitation that a purification bacterium and a clastic enzyme thereof cannot decompose the lignocellulose efficiently, provides a key technique for decomposing, saccharifying biomass containing cellulose and converting the biomass into an energy source, and has extensive application prospect in the field of decomposing the lignocellulose.

The invention relates to a method for preparing a composite heterotrophic nitrobacteria water quality modifying solid powder or granule, and belongs to the technical field of aquiculture technology and ecological environmental protection. The water quality modifying solid powder and granule is prepared by the following steps: mixing Bacillus foecalis alkaligenes CGMCC NO.1.1786 and arthrobacterium CGMCC NO.1282 into mixed bacteria; performing fermentation on the mixed bacteria for 24 to 36 hours in a culture medium in a fermenter at the fermentation temperature of between 28 and 32 DEG C; processing the obtained product into a solid powder or granule after fermentation culture; and preparing the composite heterotrophic nitrobacteria water quality modifying solid powder or granule with the functions of reducing ammonia nitrogen and nitrite nitrogen in a water body. The preparation is convenient to use, is slightly influenced by environmental conditions, has small dosage, can obviously reduce the content of the ammonia nitrogen and the nitrite nitrogen in the pool water body after use, maintains the effect for 10 to 15 days, and can effectively prevent diseases and paroxysmal death of aquaculture organisms and improve the yield and the quality of the aquaculture organisms when simultaneously improving the environmental quality of the pool water quality and the survival conditions of the aquaculture organisms.

The invention relates to a heterotrophic nitrification-aerobic denitrification compound bacterial agent for low-temperature high ammonia-nitrogen removal and application. The compound bacterial agentis formed by compounding of 10-20% of cupriavidus SWA1, 5-20% of alcaligenes faecalis, 10-30% of acinetobacter and 20-50% of ochrobacterum TAC-2. The compound bacterial agent has advantages of high ammonia-nitrogen resistance, low-temperature resistance, heavy metal resistance and synchronous nitrification and denitrification; by synergistic effects of four microorganisms, efficient denitrification of high ammonia-nitrogen wastewater can be realized in an aerobic environment under low-temperature conditions.

The invention discloses an alcaligenes faecalis and application thereof. The alcaligenes faecalis is named alcaligenes faecalis (Alcaligenesfaecalis) NR and is stored in the China General Microbiological Culture Collection Center (CGMCC) with CGMCCNo.7543 on April 28, 2013. The alcaligenes faecalis is applicable to the treatment of nitrogen-containing organic wastewater and can be used for synchronously removing nitrogen and organic carbon in the wastewater. The alcaligenesfaecalis NR has both functions of denitriding and removing the organic carbon synchronously, only needs one aerobic stage to realize the synchronous removal of the organic carbon and nitrogen, and has the removal rate of about 80 to 90% for the organic ratio, and the removal rate of about 78 to 88% for TN (Total Nitrogen).

The invention provides a composite microbial biogen capable of degrading organic waste efficiently and a preparation method and applications thereof. The composite microbial biogen contains Bacillus circulans, Bacillus subtilis, Alcaligenes faecalis and Penicillium diversum. The composite microbial biogen can be used with activated sludge for the existing sewage treatment equipment; under the synergetic effect, the yield of excess sludge can be greatly reduced, the aims of emission reduction and energy saving can be realized and the sludge problem which is difficult to overcome for a long time in sludge treatment is effectively solved; and the biogen has importanct economic importance and environmental importance and is suitable for each sewage treatment plant. The composite microbial biogen can also be used to prepare organic wastewater purifying agents, activated sludge enhancers of urban domestic wastewater and deodorants used in tipping sites, public places, agricultural product market, kitchens, bathrooms and the like.

The invention discloses a microbial preparation which comprises pseudomonas stutzeri, bacillus subtilis, lactobacillus, alcaligenes faecalis and aspergillus niger. The invention further discloses a preparation method of the microbial preparation and a method of strengthening the biochemical water treatment effects by the microbial preparation. The pseudomonas stutzeri, the bacillus subtilis, the lactobacillus, the alcaligenes faecalis and the aspergillus niger in the microbial preparation can form dominant bacterial communities in a sewage treatment system, strengthen the treatment effects of COD (chemical oxygen demand) and the like in sewage, and particularly can form advantages in a printing and dyeing sewage treatment system after matching with aniline-degrading bacteria and aerobic denitrifying bacteria, so that printing and dyeing sewage COD, aniline, chroma and total nitrogen are rapidly degraded in a targeted manner, and the printing and dyeing sewage treatment effects are strengthened.

The invention discloses a lignocellulose degrading complex bacterium system. The bacterium system consists of Alcaligenesfaecalis, Bacillusamyloliquefaciens,Bacillussubtilis and Bacillussp. The strain is preserved in China Center for Type Culture Collection, and the preservation No. is respectively CCTCC M2013168, CCTCC M2013169, CCTCC M2013170 and CCTCC M2013171. The complex bacterium system, through a synergistic effect, can comprehensively degrade cellulose, hemicellulose and other organic substances in straw, and a total degrading rate of the lignocellulose in the straw through liquid fermentation for 3 days is nearly 70%; the bacterium system is short in fermentation period which is just 3-6 days.

The invention discloses high-concentration chemical sewage composite inocula. The high-concentration chemical sewage composite inocula comprise, by weight, 180-220 parts of bacillus cereus, 150-160 parts of bacillus circulans, 75-85 parts of Exiguobacterium acetylicum, 90-120 parts of bacillus coagulans, 150-170 parts of pseudomonas aeruginosa, 20-25 parts of micrococcus luteus, 120-140 parts of thiobacillus thiooxidans, 180-230 parts of rhodococci, 20-30 parts of lactobacillus brevis, 50-65 parts of paracoccus denitrificans, 95-112 parts of alcaligenes faecalis, 110-125 parts of pseudomonas putida, 100-130 parts of enterobacter aerogenes, 280-320 parts of beer yeast and 140-160 parts of lactobacillus fermenti. All strains of the composite inocula reasonably cooperate with each other, produce synergistic effects, do not produce antagonism, have a high survival rate, is suitable for one step addition and fast forms dominant bacterial community. The high-concentration chemical sewage composite inocula have unique treatment effects on high-concentration chemical sewage, have a COD clearance rate of 94.17%, reduce an operation cost and promote qualified discharge.

The invention relates to a method for preparing a water quality modifying microecological preparation for reducing ammonia nitrogen and nitrite nitrogen, and belongs to the technical field of aquiculture technology and ecological environmental protection. The microecological is prepared by the following steps: mixing Bacillus foecalis alkaligenes CGMCC NO.1.1786 and arthrobacterium CGMCC NO.1282 into mixed bacteria; performing fermentation on the mixed bacteria for 24 to 36 hours in a culture medium in a fermenter at the fermentation temperature of between 28 and 32 DEG C; processing the obtained product into a liquid preparation through filling or continuous dilution or concentration after fermentation culture; and preparing the water quality modifying microecological preparation with the functions of reducing the ammonia nitrogen and the nitrite nitrogen in a water body. The preparation is convenient to use, is slightly influenced by environmental conditions, has small dosage, can obviously reduce the content of the ammonia nitrogen and the nitrite nitrogen in a pool water body after use, maintains the effect for 10 to 15 days, can effectively prevent diseases and paroxysmal death of aquaculture organisms and improve the yield and the quality of the aquaculture organisms when simultaneously improving the environmental quality of the pool water quality and the survival conditions of the aquaculture organisms.

The invention discloses a soil conditioner for degrading pesticides in soil with microorganisms. The soil conditioner is prepared from, by mass, 100-200 parts of organic fermented manure, 3-5 parts of trace fertilizer, 2-5 parts of bentonite, 5-7 parts of phosphate rock powder, 3-5 parts of amino acid and 0.1-1 part of microbial flora, wherein the microbial flora is composed of pseudomonas fluorescens, rhodopseudomonas palustris, alcaligenes faecalis, pseudomonas putida, bacillus licheniformis, spherical lysine bacillus, bacillus subtilis and bacillus cereus. The soil conditioner is prepared by compounding various microbial strains, the organic fermented manure, the trace fertilizer, amino acid, the bentonite and the phosphate rock powder, and can degrade the chemical pesticides in the soil, such as organic phosphorus pesticides, heterocyclic pesticides, phenol pesticides and aromatic pesticides, and remedy the pesticide-contaminated soil.

The invention provides an Alcaligenes faecalis strain M50-B1, which has a preservation number of CGMCC 5219. The invention also provides application of the bacterium in a biological desulfurization deodorant. The bacterium is prepared into a liquid bacterial agent and a solid bacterial agent. During oxidation of hydrogen sulfide, no acid is produced. At the same time, a weak acidic bacterial liquid can absorb the ammonia in the air in the spraying process, thus achieving a good deodorization effect. The activated carbon in solid bacterial agents makes up the delay defect of biological deodorization, and the deodorizing cost is reduced. The Alcaligenes faecalis provided by the invention and the biological desulfurization deodorant prepared from the same have a good deodorization effect. The desulfurization deodorant has the advantages of simple preparation method, low cost, and no secondary pollution, thus being suitable for popularization and application in deodorization of household garbage and livestock and poultry production.

The invention discloses a method for treating phenol-containing waste water by sawdust immobilized alcaligenes faecalis. The method has the advantages that (1) the immobilized alcaligenes faecalis is easy to culture and has the advantages of simple immobilization process, excellent catalytic degradation effect, resistance to high-concentration phenol-containing waste water with the concentration of phenol up to 3000mg.milligram<-1>, strong phenol decomposition capability and repeated use; (2) the carrier of the immobilized alcaligenes faecalis is sawdust, and modified utilization of the sawdust is realized by a chemical method, so the method improves the treatment efficiency for the phenol-containing waste water by means of microbial degradation, and has a broad development prospect; (3) the immobilized alcaligenes faecalis prepared through taking sawdust as the carrier has excellent elasticity, strong resistance to temperature and pH value, stable degradation property and long half life period; and (4) when used for treating the phenol-containing waste water, the sawdust immobilized alcaligenes faecalis has the advantages of simple process, low running cost, moderate conditions, environmental protection, uneasy loss of thalli, high concentration of microorganism inside the carrier, quick degradation speed, high degradation efficiency and no secondary pollution, and can realize continuous industrial degradation of the phenol-containing waste water at the same time.

The invention relates to a preparation for trinitrotoluene-contained sewage treatment and a using method of the preparation. The preparation is formed of raw materials, by weight, 20% of nitrosobacteria, 20% of bacillus subtilis, 15% of alcaligenes faecalis, 10% of pseudomonas aeruginosa, 10% of brewer's yeast, 10% of rhodococcus fascians, 10% of bacillus megatherium and 5% of aspergillus niger. Various raw materials of the preparation are reasonably prepared and symbiotically coordinated without antagonism, and accordingly, the preparation is applicable to industrial sewage treatment, treatment quality and treatment water quality can be improved, running cost can be reduced and discharge up to standards is promoted.

The embodiments of the present invention provide a composite microbial agent for treating black and odor water bodies, wherein the composite microbial agent is mainly used for treating black and odorwater bodies of urban river courses and town pit ponds, and comprises Pseudomonas sp. AW0H4, Bacillus megaterium AW0F5, Alcaligenes faecalis, Bacillus pumilus and Geobacillus stearothermophilus. According to the present invention, the single strain is obtained through a fixed screening method, wherein the microbial biomass achieves 1*10<9> cfu/mL after the single strain is activated and concentrated, and the composite microbial agent is prepared by mixing according to a ratio of 2:2:2:1:1 or 2:2:1:1:1; the storage method comprises: adding a glycerol protection agent to the single strain concentrated bacterial liquid or the composite microbial agent, and freezing; and the composite microbial agent has high specificity for black and odor water bodies, and the metabolic functions of the strains in the composite microbial agent are highly complementary to each other.

The invention discloses an efficient sewage treatment microbial agent. The efficient sewage treatment microbial agent is prepared from, by weight, 2-4 parts of nitrosobacteria, 10-20 parts of bacillus subtilis, 8-15 parts of alcaligenes faecalis, 5-10 parts of bacillus megatherium, 2-8 parts of pseudomonas aeruginosa, 2-10 parts of candida lipolytica, 2-8 parts of deoxidized paracoccus, 2-6 parts of pseudomonas stutzeri, 2-10 parts of saccharomyces cerevisiae, 1-4 parts of aspergillus niger, 0.1-0.6 part of yeast extract, 2-6 parts of peptone, 0.1-0.5 part of glucose, 8-15 parts of kaolin, 2-5 parts of aluminum polychlorid, 0.1-0.5 part of FMEE and a proper amount of water. The efficient sewage treatment microbial agent is suitable for treating industrial waste water, breeding waste water and the like, all strains are in cooperation, symbiotic coordination is achieved, high activity is achieved, and reproduction is fast. The efficient sewage treatment microbial agent is put in the waste water to be treated, the degradation effect can be effectively accelerated, the treatment water quality can be greatly improved, operating cost can be reduced, the treated water reaches the standard to be drained, and damage to environment is reduced.

The invention belongs to the technical field of wastewater treatment and particularly relates to a microbial agent suitable for treating urban wastewater and a preparation method thereof. The microbial agent comprises Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, saccharomycetes, lactic acid bacteria, acetic acid bacteria, photosynthetic bacteria, Actinobacteria, indigenous bacteria, nitrifying bacteria, denitrifying bacteria, phosphorus-accumulating bacteria, Rhodospirillum, Thiobacillus denitrificans, Sphingobacteria, Alcaligenes faecalis, Bifidobacterium, brown rot fungi,Geotrichum candidum, Mucor, Aspergillus oryzae, and Penicillium diversum. The preparation method includes: mixing well Actinobacteria, indigenous bacteria, nitrifying bacteria, denitrifying bacteria,phosphorus-accumulating bacteria and Bifidobacterium, adding the mixture into clean soil, farming earthworms with the soil for 12-14 days, and isolating the earthworms and the soil to obtain earthwormcast containing the mixed bacteria; mixing the earthworm cast containing the mixed bacteria and other bacteria, and immobilizing to obtain the microbial agent.

The invention belongs to the field of microbial application, and particularly relates to alcaligenes faecalis and application thereof. According to the specific technical scheme, the alcaligenes faecalis is preserved in China General Microbiological Culture Collection Center on June 11, 2018, the strain name is alcaligenes faecalis DFN2, and the preservation number is CGMCC No.15926. The alcaligenes faecalis has an excellent degradation effect on common malodorous substances such as ammonia gas and hydrogen sulfide, and the maximum degradation rate of the ammonia gas and the hydrogen sulfide can reach 100%; and the degradation rate of an ammonia nitrogen substance in high-ammonia-nitrogen wastewater can reach up to 99.5%. The alcaligenes faecalis can also carry out biological desulfurization treatment on thiophene sulfide, and the desulfurization effect is obviously superior to a universal hydrodesulfurization effect.

The invention discloses a method for producing diglycolamidic acid, namely, the key intermediate synthesized by glyphosate through biological catalysis and a new strain used in the process. The method takes iminodiacetonitrile as a raw material and a microbial enzyme with nitrilase activity obtained from the fermentation culture of Alcaligenes faecalis ZJUTB10 as a catalyst and obtains the diglycolamidic acid as a product by going through hydrolysis reaction under certain condition. The beneficial effects of the method are mainly reflected by little waste water produced during the reaction process, little pollution, mild reaction conditions, low energy consumption, high conversion rate and high yield and being easy for industrialized production.