46 results about "Alcaligenes faecalis" patented technology

The invention relates to the technical field of microbial deodorants, and discloses a microbial compound bacterium deodorant and a preparation method thereof.The microbial compound bacterium deodorant comprises a bacterium powder mixture, the bacterium powder mixture is embedded into a loading material, and the loading material is wrapped with a protective agent; the bacterial powder mixture comprises pediococcus acidilactici, bacillus megaterium, alcaligenes faecalis, bacillus subtilis and azotobacter chroococcum, wherein the mass ratio of the pediococcus acidilactici to the bacillus megaterium to the alcaligenes faecalis to the bacillus subtilis to the azotobacter chroococcum is 1: (1-3): (1-2): (0.5-1): (1-2). According to the microbial compound bacterium deodorant and the preparation method thereof, the prepared microbial compound bacterium deodorant can remarkably remove malodorous gases such as NH3 and H2S in biological excrement, the action time is long, the effect is remarkable, meanwhile, the raw materials of the microbial compound bacterium deodorant are green and safe, and the health problem caused by mistaken eating of livestock is solved.

The present invention relates to the technical field of microbial deodorants, and discloses a microbial composite deodorant and a preparation method thereof. The microbial composite deodorant comprises a bacterial powder mixture, which is embedded in a load material, and the load material is externally wrapped with a protective agent. The bacterial powder mixture comprises lactic acid Pediococcus, Bacillus megaterium, Alcaligenes faecalis, Bacillus subtilis, and Azotobacter chrysogenum, wherein the mass ratio of lactic acid Pediococcus, Bacillus megaterium, Alcaligenes faecalis, Bacillus subtilis, and Azotobacter chrysogenum is 1:1-3:1-2:0.5-1:1-2. The present invention adopts the above-mentioned microbial composite deodorant and a preparation method thereof. The prepared microbial composite deodorant can significantly remove malodorous gases such as NH3 and H2S in biological feces, has a long action time, and has a significant effect. At the same time, the raw materials of the microbial composite deodorant are green and safe, reducing health problems caused by accidental ingestion by livestock.

The invention discloses an efficient cellulose degrading bacterial agent and a preparation method and application thereof, and belongs to the technical field of microorganisms, the degrading bacterial agent comprises brevundimonas sp., alcaligenes faecalis AFBII and Achromobacter AFBIII, a genome of the brevundimonas AFBI comprises a nucleotide sequence as shown in SEQ ID NO.1, a genome of the AFBI comprises a nucleotide sequence as shown in SEQ ID NO.2, a genome of the AFBII comprises a nucleotide sequence as shown in SEQ ID NO.3, and a genome of the AFBIII comprises a nucleotide sequence as shown in SEQ ID NO.4. The genome of the alcaligenes faecalis AFBII comprises a nucleotide sequence as shown in SEQ ID NO.2, and the genome of the achromobacter AFBIII comprises a nucleotide sequence as shown in SEQ ID NO.3. The alcaligenes faecalis AFBII and the achromobacter AFBIII are used for preparing the alcaligenes faecalis According to the high-efficiency cellulose degrading bacterial agent provided by the invention, all strains have a synergistic effect, and cellulose can be efficiently and stably degraded.

The invention relates to the technical field of kitchen waste treatment, in particular to a solid fungicide, a preparation method and application. The solid microbial inoculum comprises a microbial inoculum and a carrier; the microbial agent is prepared from pseudoochrobactrum saccharum, alcaligenes faecalis, achromobacter xylosoxidans, paenibacillus amyloliquefaciens, bacillus cereus, sphingobium nicotianae, serratia marcescens, stenotrophomonas maltophilia, sphingobium humus, sphingobium aquaticum and bacillus subtilis; the carrier for carrying the liquid compound bacteria is bagasse, and the maximum number of released bacteria is 2.2 * 10 < 9 > CFU / g. When the solid microbial inoculum is added into a 18 L kitchen waste reactor, the waste decrement rate is 80% within 7 days, and the COD removal rate can reach 79.1% at most. According to the solid microbial agent disclosed by the invention, organic substances in the kitchen waste can be degraded while the kitchen waste is converted from a solid state to a liquid state, so that volume reduction and weight reduction of the kitchen waste are realized; and the solid microbial inoculum is convenient to transport and operate and low in cost, has positive significance on difficulties faced by kitchen waste treatment, and has a good application prospect.

The invention discloses an alcaligenes faecalis UA strain, a product and application thereof, and a method for repairing an acid mine environment by acid-resistant urease-producing bacteria, and belongs to the technical fields of industrial microorganisms, microorganism application materials and environment repairing. The UA strain CGMCC No.33976 is found for the first time to produce the acid-resistant urease, the urease is stably produced in the environment with the optimum acidity of pH 4.5-pH 6.5 and the initial pH of 3.5-pH 7.5, and the enzyme production activity is the highest in the environment with the initial pH of 5.5-pH 6.5. The UA strain can effectively reduce acidity and remove metal ions in water in an acid mine wastewater real water sample, the removal rate of aluminum, manganese, zinc and cadmium exceeds 99%, and the removal rate of calcium, iron and lead is 92%-98%. The UA strain is screened from the acid mine environment, has biological safety, has industrial purposes of AMD in-situ direct treatment and acid mine environment in-situ remediation, does not need to add chemical agents to adjust the pH value of the environment, and has ecological safety and environmental sustainability.

The invention discloses a complex microbial inoculant for promoting lignocellulose degradation and a preparation method and application thereof, and relates to the technical field of microorganisms, the complex microbial inoculant comprises brevundimonas sp. AFBI (Brevundimonas sp.), alcaligenes faecalis AFBII (Alcaligenes faecalis), Achromobacter AFBIII (Achromobacter sp.), Staphylococcus aureus GBSI (Staphylococcus sp.), Glutamic Bacter GBS II (Glutamic Bacter sp.) and Bacillus (Bacillus sp.). According to the composite microbial agent provided by the invention, all strains have no antagonistic action and are mutually cooperated, and lignocellulose can be efficiently and stably degraded.

The invention discloses alcaligenes faecalis X4 and application thereof, the strain is preserved in China Center for Type Culture Collection (CCTCC) on May 8, 2025, the address is Wuhan University, Wuhan, China, and the preservation number is CCTCC M 2025998. The degradation rate of the alcaligenes faecalis X4 to flumorph with the concentration of 50 mg / L exceeds 70% within 72 h, and the maximum tolerance concentration to cadmium is 120 mg / L. The strain provides powerful technical support for treatment of combined pollution of flumorph and cadmium, and has important significance for solving the problem of complex environmental pollution.

The invention discloses an agricultural soil improvement agent and a preparation method of the agricultural soil improvement agent, and belongs to the technical field of soil improvement agents. Comprising the following raw materials: 30-40 parts of soybean meal, 20-25 parts of bagasse, 15-20 parts of rice hull ash, 5-15 parts of brewer's grains, 6-12 parts of tea residues, 5-15 parts of lotus root starch, 8-10 parts of a compound microbial agent, 2-5 parts of trehalose, 15-25 parts of bentonite, 6-8 parts of turmeric powder and 5-10 parts of an inorganic chelating agent, and the compound microbial agent comprises bacillus, xylose oxidation alcaligenes denitrifying subspecies and alcaligenes faecalis. According to the invention, wastes in multiple industries are used as raw materials, the cost is reduced, neutralization of acidity is synchronously realized through biological and chemical dual effects, accumulation of nitrate and organic acids in soil is reduced through cooperation of different strains, and the synergistic effects of rapid improvement and long-acting maintenance of soil acidity and alkalinity are achieved through a dual bacteriostatic system of curcuma powder bacteriostasis and microbial bacteriostasis.

The invention provides a compound microbial agent and application thereof in regulating aquaculture water quality, and the compound microbial agent is prepared by the following steps: firstly, embedding a bacterial liquid composed of alcaligenes faecalis, pseudomonas, ochrobactrum and sphingosine box bacteria through self-assembly by adopting gallic acyl quinic acid, and then modifying lignin by using ferrocene for composite loading. And immersing into a bacterial liquid consisting of photosynthetic bacteria, bacillus and nitrifying bacteria, and drying. According to the invention, ferrocene modified lignin and galloylquinic acid construct a multi-layer load structure to fix microorganisms in a layered manner, so that the microorganisms can stably survive in a complex water body environment, and meanwhile, a plurality of florae act synergistically to degrade organic matters, nitrogen, phosphorus, antibiotics and the like in aquaculture water, so that the degradation efficiency of water pollutants can be remarkably improved; effective adjustment of aquaculture water quality is facilitated, and good data support and theoretical basis are provided for water body improvement in the field of aquatic products.

The invention relates to the technical field of water treatment agents, in particular to a biological conditioner with water quality purification capacity and a preparation method and application thereof, and the biological conditioner is composed of a modified porous aluminosilicate inorganic material, an active microbial agent, fermentation liquor of the active microbial agent and an embedding material. The modified porous aluminosilicate inorganic material is diatomite, modified zeolite or perlite; the active microbial agent comprises bacillus, photosynthetic bacteria, alcaligenes faecalis, pseudomonas and nitrifying bacteria. The biological conditioner contains multiple beneficial microorganisms such as photosynthetic bacteria, nitrifying bacteria and bacillus, the microorganisms and multiple enzymes generated by the microorganisms have a synergistic effect, the respective purification effect can be achieved in a water body, harmful substances in the water body are synergistically degraded, and the self-purification capacity of the water body is improved.

The invention relates to the technical field of straw microbiological treatment, and discloses a composite microbial preparation for straw treatment and a preparation method. The composite microbial preparation for straw treatment is prepared by mixing a microbial group formed by pseudomonas aeruginosa, aspergillus niger, trichoderma reesei, alcaligenes faecalis and medium and low temperature pectinase with probiotics and a nutrient solution in proportion, the volume ratio of the pseudomonas aeruginosa to the aspergillus niger to the trichoderma reesei to the alcaligenes faecalis to the medium and low temperature pectinase is (1-1.5): (1-2): (0.5-1): (1.5-2): (0.5-2.5), the probiotics account for 2%-6% of the microbial group, and the mass ratio of the probiotics to the nutrient solution is 1: (20-30). The microbial preparation disclosed by the invention can well adapt to decomposition treatment of straws in cold regions, induces enrichment of aerobic bacteria, improves soil conditions, is more beneficial to survival and reproduction of the composite microbial preparation, and optimizes the decomposition treatment effect on the straws.

The invention discloses alcaligenes faecalis and an application thereof in disease prevention and growth promotion, the strain is preserved in the China General Microbiological Culture Collection Center on November 1, 2023, the preservation number is CGMCC No.28835, and the preservation name is alcaligenes faecalis WYH-571. The alcaligenes faecalis has the advantages that the preservation number is CGMCC No.28835, and the alcaligenes faecalis WYH-571; when being applied to the field of agricultural biological prevention and control, the strain can form a biological membrane to be stably colonized at the rhizosphere of a plant, and can generate substances such as IAA and siderophores to promote the growth of the plant.

The invention relates to the technical field of microorganisms and compositions thereof, and particularly discloses a preparation method and application of a saline-alkaline tolerant complex microbial inoculant. The saline-alkaline tolerant complex microbial inoculant is prepared from the following raw materials in parts by weight: 15-20 parts of an arbuscular mycorrhizal fungal spore mixture; 20 to 25 parts of a growth-promoting bacterium solution; 60-70 parts of a matrix; 4-5 parts of a nutrient substance; a strain in the growth-promoting bacterium liquid comprises one or more of enterobacter luviveilis, serratia marcescens and alcaligenes faecalis, and the matrix comprises common biochar and diatomite, and the microelement comprises one or more of the enterobacter luviveilis, the serratia marcescens and the alcaligenes faecalis. The preparation method comprises the following steps: S1, preparing a bacterial liquid; s2, carrier preparation; and S3, mixed fermentation. The product can be used for improving saline-alkali soil, the application amount is 2-4 kg / hm < 2 >, the product has the advantages of being high in survival rate and activity, and the organic matter content of soil and the survival rate of plants in saline-alkali soil can be effectively increased.

The present invention discloses a method for preparing β-glucose oligosaccharides, and relates to the field of fermentation technology. The method comprises the following steps: (1) preparing a Bacillus faecalis seed solution; (2) preparing a Trichoderma LE02 seed solution; (3) fermenting: inoculating the Bacillus faecalis seed solution into a fermentation medium, and inoculating the Trichoderma LE02 seed solution once at 8-12 hours, 45-55 hours, and 85-95 hours after the inoculation of the Bacillus faecalis seed solution, respectively. The method is ventilated and fermented for 120 hours to obtain a β-1,3-glucose oligosaccharide fermentation liquid; and (4) purifying. The present invention, by extending the fermentation time or adding the Trichoderma LE02 seed solution in batches, ultimately obtains a thermogelling oligosaccharide β-1,3-glucose oligosaccharide with a lower degree of polymerization and a higher concentration, with a yield of up to 89-93%, which can be industrialized.

This invention discloses a creatine amidoside hydrolase mutant for creatinine detection, along with methods for selecting, purifying, and detecting the mutant site. The mutant is a single-point mutation of the wild-type full-length amino acid sequence of the creatine amidoside hydrolase protein (Af-CRE) from Alcaligenes faecalis. This invention utilizes machine learning-assisted site-directed mutagenesis to create a creatine amidoside hydrolase mutant with enhanced activity, obtaining a mutant enzyme with improved activity and certain thermostability. Furthermore, it provides methods for purifying and detecting the mutant, overcoming the shortcomings of existing creatine amidoside hydrolases with poor activity that cannot meet the requirements for reagent applications. This lays the foundation for further expanding the industrial applications of creatine amidoside hydrolases.

The present application relates to the technical field of fertilizer, in particular to a kind of microbial compound inoculant, method and application for promoting the fast maturation of organic fertilizer;The method of the present application comprises inoculating microbial compound inoculant formed by Brevundimonas AFB I, Alcaligenes faecalis AFB II and achromobacter AFB III to compost containing cow dung and corn straw, and carrying out fermentation under natural conditions, the effective bacteria number ratio of Brevundimonas AFB I, Alcaligenes faecalis AFB II and achromobacter AFB III in microbial compound inoculant is 1:1:1, and cow dung and straw are mixed according to dry weight 3:1;The microbial compound inoculant developed by the present application can significantly improve the degradation efficiency of organic waste, accelerate the composting process, shorten the composting period, and improve the stability and fertility of compost products, providing new technical support for the resource utilization of agricultural waste.

The present invention discloses the UA strain of Alcaligenes faecalis, its products and applications, and a method for remediating acidic mine environments using acid-resistant urease-producing bacteria. These strains belong to the fields of industrial microorganisms, microbial application materials, and environmental remediation. The UA strain, CGMCC No. 33976, is the first acid-resistant urease-producing bacteria discovered. Its optimal acidity lies between pH 4.5 and pH 6.5, with stable urease production at an initial pH of 3.5 to pH 7.5 and highest enzyme activity at an initial pH of 5.5 to pH 6.5. In real samples of acidic mine wastewater, the UA strain effectively reduces acidity and removes metal ions, achieving removal rates exceeding 99% for aluminum, manganese, zinc, and cadmium, and 92% to 98% for calcium, iron, and lead. The UA strain, screened from acidic mine environments, exhibits biosafety and is suitable for in-situ direct treatment of AMD and in-situ remediation of acidic mine environments. It eliminates the need for chemical pH adjustment, ensuring ecological safety and environmental sustainability.

The invention discloses alcaligenes faecalis NRM3 and application of the alcaligenes faecalis NRM3 in prevention and treatment of tobacco root rot, and belongs to the technical field of biological prevention and treatment of tobacco diseases. The alcaligenes faecalis NRM3 is separated from tobacco, is a tobacco endophytic bacterium and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20251578. The alcaligenes faecalis NRM3 can effectively inhibit the growth of pathogenic bacteria of the tobacco root rot, can effectively prevent and treat the tobacco root rot, can remarkably reduce the morbidity and the disease index of diseased plants, also has a positive promotion effect on the germination and growth of tobacco seeds, and has good application potential in the tobacco industry.

This invention belongs to the field of microbial control technology, specifically relating to a biocontrol bacterium for soybean root rot, a microbial agent, its preparation method, and its application, which can improve soybean yield and oil content. The biocontrol bacterium is *Alcaligenes faecalis* (Factophytes). Alcaligenes faecalis A new strain, named GY16, was deposited at the China Center for Type Culture Collection (CCTCC) on November 12, 2025, with accession number CCTCC M 20252536. This alkali-producing bacterium, GY16, exhibits a specific affinity for soybean, enabling it to colonize soybean roots and effectively control soybean root rot. GY16 also promotes soybean plant growth, increases soybean yield, and improves seed oil content. Inoculants prepared using GY16 can replace pesticides, offering a green, environmentally friendly, and pollution-free alternative that positively contributes to building a sustainable farmland ecosystem.

The invention relates to a preparation method and application of an anti-Parkinson hydrogel preparation, solves the problems of oxidative stress of the brain of Parkinson and dysbacteriosis of intestinal flora, and adopts the technical scheme that the anti-Parkinson hydrogel preparation is prepared by the following steps: extracting bacterial outer membrane vesicles of alcaligenes faecalis through an ultracentrifugation method; the preparation method comprises the following steps: complexing cerium ions and polyphenol substances into a metal polyphenol network, loading the cerium metal polyphenol network into bacterial outer membrane vesicles by a mechanical extrusion method, and finally mixing the substances with inulin to obtain the anti-Parkinson hydrogel preparation. The preparation conditions are mild, the process is simple, the method is stable and reliable, the production cost is low, a new thought for treating the Parkinson's disease is developed, and the traditional Chinese medicine composition is an innovation for treating the Parkinson's disease and has huge economic and social benefits.

The application discloses a method for high-efficiency degradation of high-salinity wastewater by salt-tolerant bacteria in a composite inorganic salt system, and relates to the technical field of biological treatment of high-salinity industrial wastewater.The method realizes directional strengthening of the salt-tolerant performance of three strains of Staphylococcus xylosus, Alcaligenes faecalis and Bacillus cereus by a gradient-increasing NaCl domestication method.Under the condition of 40-50 g / L high salinity, the growth amount of the domesticated strains is increased by 30-40% compared with that of the non-domesticated strains, effectively relieving the osmotic pressure stress and metabolic enzyme activity inhibition of the microorganisms in the high-salinity environment.Meanwhile, the composite salt-tolerant bacteria agent is compounded based on functional complementation, and the agent has the abilities of efficient denitrification, phosphorus removal and organic matter degradation, and can adapt to the composite inorganic salt system of NaCl, CaCl2 and MgSO4 alone or in combination, and can realize synchronous and efficient removal of COD, ammonia nitrogen, total nitrogen and total phosphorus in a wide salinity range of 10-80 g / L.The method breaks through the technical limitation that the existing salt-tolerant bacteria only adapt to a single salt system and have single degradation function, and greatly widens the applicable scenarios of the biological method in the treatment of high-salinity wastewater.

This invention relates to the field of microbial technology, specifically disclosing a strain of *Alcaligenes faecalis* ALA-1 and its application in nitrous oxide emission reduction. This invention provides a strain of *Alcaligenes faecalis* (… Alcaligenes faecalis ALA-1, with accession number CGMCC No. 35322, can efficiently convert N... 2 O is restored to N 2 When applied to aerobic composting processes, it can significantly reduce nitrogen emissions during the composting process. 2 O2 emissions will promote the green and low-carbon development of the organic solid waste composting industry.

The invention discloses a preparation, separation and purification method of glucan oligosaccharide, and belongs to the technical field of active oligosaccharide preparation. The preparation, separation and purification method comprises the following steps: adding alcaligenes faecalis into a fermentation culture medium, fermenting to prepare beta-1, 3-glucan, adding beta-1, 3-glucanase when the viscosity of fermentation liquor reaches a certain value, and simultaneously controlling the temperature to promote enzymolysis, so that the beta-1, 3-glucan with high polymerization degree is hydrolyzed into oligosaccharide, and separating and purifying the oligosaccharide. The material liquid viscosity is reduced, and the mass transfer is improved, so that the single-batch fermentation yield is improved. Meanwhile, sugars with different polymerization degrees are separated through a graded alcohol precipitation process, part of impurities are removed, and the product purity is improved. According to the method, the enzymolysis reaction is directly carried out in the fermentation process, so that the steps of polysaccharide extraction and enzymolysis are simplified, the production energy consumption is reduced, and the yield and the production benefit of the final product are improved.

This invention belongs to the field of bio-fermentation technology, and particularly relates to fermenting bacteria, a fermentation compound agent prepared based on these fermenting bacteria, and specific preparation methods and applications of the fermentation compound agent. The fermenting bacteria are selected from *Bacillus licheniformis* CY-1 and *Alcaligenes faecalis* CY-2; the *Bacillus licheniformis* CY-1 is deposited at CGMCC with accession number CGMCC No. 21241; the *Alcaligenes faecalis* CY-2 is deposited at CGMCC with accession number CGMCC No. 21242. This invention achieves effective composting of easily perishable waste through the specific combination of *Bacillus licheniformis* CY-1 and *Alcaligenes faecalis* CY-2. During the composting process, it effectively fixes nitrogen and reduces emissions, improving the quality, economic value, and utility of the treated easily perishable waste compost product.

The invention relates to a preparation method and application of an oral vaccine based on bacterial outer membrane vesicles, which can be effectively applied to preparation of drugs for preventing or treating intestinal mucosa pathogen infection and overcome the problems of poor compliance and low mucosa immune response of traditional injection vaccines. The preparation method comprises the following steps: firstly preparing bacterial outer membrane vesicles of alcaligenes faecalis, then preparing DSPE-PEG-antigens, mixing the bacterial outer membrane vesicles and the DSPE-PEG-antigens, stirring to prepare OMV-DSPE-PEG-antigens, dissolving inulin in PBS, heating, oscillating, cooling to room temperature, adding the OMV-DSPE-PEG-antigens, stirring, and standing to obtain the finished oral vaccine. The preparation process is simple, the method is stable and reliable, operation is easy, the production cost is low, the prepared oral vaccine based on the bacterial outer membrane capsule can effectively stimulate immune response of intestinal mucosa and prevent infection of mucosa pathogens, and economic and social benefits are remarkable.

The invention discloses alcaligenes faecalis capable of degrading ammonia nitrogen under complex conditions and application of the alcaligenes faecalis, and belongs to the field of microorganisms and fermentation engineering. According to the invention, a strain of Alcaligenes faecalis BL-WDN1 with excellent ammonia nitrogen reduction performance is separated from a sewage sample of an aerobic tank of a pig farm. The alcaligenes faecalis BL-WDN1 disclosed by the invention can still have a remarkable ammonia nitrogen reducing effect under the conditions of high COD (Chemical Oxygen Demand) (the content is greater than or equal to 4000 mg / L) and high ammonia nitrogen (the content is greater than or equal to 650 mg / L); more prominently, under the complex condition of simulating actual sewage degradation and jointly formed with a low-temperature and low-external carbon source, the composite material still has obvious ammonia nitrogen and COD (Chemical Oxygen Demand) degradation effect. The invention provides a brand new technical thought for the field of aquaculture sewage treatment, and the restriction of complex conditions such as high COD, low temperature, low external carbon source and the like on the ammonia nitrogen degradation effect is expected to be broken through by reasonable addition in proper links.

This invention belongs to the field of microbial technology, specifically relating to a strain of direct ammonia-oxidizing fecal alkalogen AP3-14 and its applications. The fecal alkalogen provided by this invention (… Alcaligenes faecalis AP3-14 was deposited on November 17, 2025, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 36650, located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. This strain not only possesses direct ammonia oxidation capabilities, achieving efficient denitrification under aerobic conditions through this pathway, but also simultaneously degrades ammonia nitrogen and phenol in wastewater or sewage. Furthermore, this strain can tolerate a wide range of ammonia nitrogen concentrations, phenol concentrations, and pH levels.

The application provides a method for removing hexavalent chromium by using the interaction between microorganisms and copper ions, comprising the following steps: S1, providing microorganisms, wherein the microorganisms comprise Alcaligenes faecalis; S2, obtaining a treatment liquid with a combined system; the treatment liquid contains hexavalent chromium and divalent copper ions; wherein the initial concentration of the hexavalent chromium in the treatment liquid is 50-500 mg / L, and the initial concentration of the divalent copper ions in the treatment liquid is 10-100 mg / L; and S3, culturing the microorganisms in the treatment liquid to remove the hexavalent chromium in the treatment liquid. The application can quickly and efficiently remove the hexavalent chromium in water bodies.

The present invention discloses a creatine amidinohydrolase mutant with higher thermal stability and its application, relating to the field of biotechnology, including mutants V283L, H193Y, E170T, S19L, Y310L, Q151V and / or mutant A180K; their amino acid sequences are shown in SEQ ID NO.2-SEQ ID NO.8 respectively. A method for selecting mutation sites, using deep learning to transform target scoring for the wild type of Creatniase protein of Alcaligenes faecalis; and selecting target sites by structural co-evolution analysis assisted by the crystal structure of the enzyme or homology modeling. Application of the mutant in creatinine detection reagents. The present invention produces a creatine amidinohydrolase mutant with improved thermal stability by site-directed mutagenesis assisted by machine learning.

The invention belongs to the technical field of microorganisms, and discloses alcaligenes faecalis and application thereof in prevention and treatment of root rot of schisandra chinensis. The invention provides a strain of alcaligenes faecalis, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.32518. The alcaligenes faecalis provided by the invention has a relatively good prevention and treatment effect on schisandra chinensis root rot.