Polyvinyl alcohol coated chromatographic stationary phase and its preparation method and application

A chromatographic stationary phase and polyvinyl alcohol technology, which is applied in the field of polyvinyl alcohol-coated chromatographic stationary phases, can solve the problems of complicated stationary phase preparation process, influence on the reproducibility of analysis results, limited pH applicable range, etc., and achieves technical application The effect of a wide range, novel design ideas, good retention and separation characteristics
CN104226283BInactive Publication Date: 2017-03-29EAST CHINA UNIV OF SCI & TECH
2 Cites 2 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
EAST CHINA UNIV OF SCI & TECH
Publication Date
2017-03-29
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a polyvinyl alcohol coating chromatography stationary phase as well as a preparation method and application thereof. The preparation method comprises the following steps: a, preparing a polyvinyl alcohol aqueous solution; b, coating the surface of each spherical particle, namely, adding the spherical particles into the polyvinyl alcohol aqueous solution obtained in the step a, stirring the spherical articles at 300 to 1500rpm so as to uniformly mix the spherical articles and the aqueous solution; and c, washing and drying, namely, filtering a reaction system in the step b, washing a solid product by utilizing water, and performing vacuum drying on the solid product to be used as packing stuffing. The polyvinyl alcohol coating chromatography stationary phase and the preparation method have the advantages that the coating-type material is prepared by utilizing a physical coating principle, and the design concept is novel and environment-friendly. The coating process is completed in the aqueous solution system, the operation process is simple and convenient, and the reaction condition is moderate. The technology of the preparation method is wide in application range, a compound of the needed functional group is added in the polyvinyl alcohol aqueous solution to perform the coating, the stationary phase of any function can be prepared, and a wide development space is provided for the polyvinyl alcohol immobilization stationary phase.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of hydrophilic chromatographic stationary phases, in particular to a polyvinyl alcohol-coated chromatographic stationary phase. Background technique

[0002] Hydrophilic interaction chromatography (HILIC) was first proposed by Alpert in 1990. Its basic feature is that it adopts a stationary phase similar to normal phase chromatography, and uses a mobile phase similar to reversed phase chromatography. It can effectively solve the shortcomings of reversed-phase chromatography that cannot effectively separate highly polar compounds, and at the same time overcome the defect of low solubility of polar compounds in normal phase chromatography mode, and is widely used for the separation of highly polar compounds. In addition, its high proportion of organic phase makes it compatible with mass spectrometry, which improves the detection sensitivity of separation and analysis.

[0003] As a key component of hydrophilic interac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More