Saccharomyces cerevisiae mutant bacterial strain and use thereof in glutathion production by fermentation

A mutant strain, Saccharomyces cerevisiae technology, applied in fermentation, microbial-based methods, enzymes, etc., can solve the problems of limited biomass accumulation, reduced microbial fermentation capacity, and unstable yeast quality, achieving reduction in bacterial contamination, Good pH tolerance, beneficial to large-scale production
CN101407768AInactive Publication Date: 2009-04-15北京赫芙德食品科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
北京赫芙德食品科技有限公司
Publication Date
2009-04-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of biological engineering, and relates to a new saccharomyces cerevisiae strain mutant with high yield of glutathione and the class name of Saccharomyces cerevisiae Y518, and application of Gamma-glutamyl cysteine synthetase gene and glutathione synthetase gene that are generated by the strain, and the strain application for glutathione preparation. An original NTG saccharomyces cerevisiae strain with continuous induction is inductively processed for 4 times, thus obtaining the saccharomyces cerevisiae strain mutant Y518. As Gamma-glutamyl cysteine synthetase and glutathione synthetase that are generated by the saccharomyces cerevisiae strain Y518 experience point mutation, compared with an original strain, the saccharomyces cerevisiae Y518 has stronger acid resistance, stronger pH tolerance, good stability and remarkably raised yield of glutathione. The glutathione preparation that utilizes the saccharomyces cerevisiae Y518 is characterized by simple operation, stability, good repeatability, high yield and the like, generates stable and cheap glutathione, and is favorable for scaled glutathione production.
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Description

technical field

[0001] The invention belongs to the technical field of bioengineering, in particular to a mutant strain Saccharomycescerevisiae Y518 of Saccharomyces cerevisiae, which simultaneously produces gamma-glutamylcysteine ​​synthetase and glutathione synthetase, and the genes of the two enzymes All have point mutations, and the present invention also relates to the application of the mutant strain Saccharomyces cerevisiae Y518 in the production of glutathione by fermentation. Background technique

[0002] Glutathione (GSH) is a biologically active tripeptide compound formed by condensation of L-glutamic acid, L-cysteine ​​and glycine through peptide bonds, and is a natural antioxidant. GSH was first isolated in 1888 and officially named in 1921. GSH widely exists in organisms such as animals, plants and microorganisms. There are many documents (Tateishi N., 1974.J.B.75:93~103; Issels R., 1988.Biochem Pharmacol.37:881~888; Meister.A., 1983.Ann.Rev.Biochem.52:711 ~...

Claims

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